Objective To investigate the correlations of serum dual-specific phosphatase 1(DUSP1)and soluble cluster of differentiation 93(sCD93)with severity of disease,lung function and airway inflammation in children with acute-phase bronchial asthma(BA).Methods A total of 100 children with acute-phase BA(acute-phase group),100 children with remission-phase BA(remis-sion-phase group),and 100 healthy children(control group)in the Xingyuan Hospital of Yulin from January 2021 to January 2023 were selected,and the acute-phase group was further divided into mild subgroup(n=47),moderate subgroup(n=36)and severe subgroup(n=17)according to the se-verity of the disease.Serum DUSP1,sCD93 levels and lung function indexes[peak expiratory flow(PEF),percentage of forced expiratory volume in the first second compared to the expected value(FEV1％pred)],and airway inflammation indexes[eosinophil(EOS)count,fractional exhaled nitric oxide(FeNO)]were measured.Pearson's method was used to analyze the correlations of serum DUSP1 and sCD93 levels with PEF,FEV1％pred,EOS and FeNO in children with acute-phase BA.Results The levels of serum DUSP1,PEF,and FEV1％pred in the acute-phase group were signifi-cantly lower than those in the remission-phase group and the control group,while the levels of sCD93,EOS count and FeNO were significantly higher than those in the remission-phase group and the control group(P＜0.01).The levels of serum DUSP1,PEF and FEV1％pred in the severe subgroup were significantly lower than those in the moderate and mild subgroups,while the levels of sCD93,EOS count and FeNO were significantly higher than those in the moderate and mild sub-groups(P＜0.01).PEF and FEV1％pred were positively correlated with serum DUSP1 level,but negatively correlated with sCD93 level in children with acute-phase BA(P＜0.01).EOS count and FeNO were negatively correlated with serum DUSP1 level,but positively correlated with sCD93 level(P＜0.01).Conclusions The decreased serum DUSP1 level and increased sCD93 level in chil-dren with acute-phase BA are closely related to the aggravation of the disease,the decrease of lung function,and airway inflammation,which may become new targets for the treatment of children with acute-phase BA.

This paper summarized the experience of Professor FANG Dingya in staged treatment of Sjögren's syndrome from the perspective of dryness toxin. It is believed that the cause of Sjögren's syndrome is externally-contracted dryness， consumption of essence and fluid， congenital and acquired essence deficiency， depleted essence and insufficient blood， and the core mechanism is internal accumulation of dryness toxin. The treatment can be divided into three stages， that is dryness toxin transforming into fire-heat， damp-heat and phlegm-stasis， from the perspective of dryness metal qi transformation. It is emphasized to dispel pathogen mainly， to clear and moisten with yin-nourishing medicinals in supplementation， and to treat by stages based on syndrome differentiation. For dryness toxin with fire-heat， it is suggested to moisten dryness， resolve toxins and subdue fire， with self-made Runzao Jiedu Decoction （润燥解毒汤） in modification. For dryness toxin with damp-heat， the method of nourishing yin， clearing heat and draining dampness should be used， and Chunze Decoction （春泽汤） in modification is suggested. For dryness toxin with phlegm-stasis， it is recommended to unblock collaterals， disperse phlegm and dissipate stasis， with self-made Sanyu Xiaotan Decoction （散瘀消痰汤） in modification.

Purpose To explore the influence of different spin-locking frequencies on T1ρ values based on a 3.0T MR system.Materials and Methods Thirty-eight healthy adult volunteers underwent cardiac magnetic resonance imaging at the First Affiliated Hospital of Anhui Medical University from July to September 2023.T1ρ mapping and short-axis cine imaging with steady-state free precession sequences were performed with 3.0T MR system.T1ρ mapping sequence in three short-axis slices with three spin-lock frequencies at the amplitude of 5 Hz,300 Hz,400 Hz,and 500 Hz was scanned,respectively.T1ρ relaxation times and myocardial fibrosis index were quantified for each slice and each myocardial segment,the difference in T1ρ of different spin-locking frequencies and myocardial fibrosis index was analyzed using one-way repeated-measures analysis of variance method.Results T1ρ of 5 Hz,300 Hz,400 Hz,and 500 Hz were(33.9±2.8)ms,(43.4±2.1)ms,(45.4±2.6)ms and(46.5±2.4)ms,respectively;and T1ρ values showed a significant progressive increase from the low spin-lock frequency to the high spin-lock frequency of the heart(300 Hz vs.400 Hz:P<0.001;300 Hz vs.500 Hz:P<0.001;400 Hz vs.500 Hz:P=0.043).In addition,the measured myocardial fibrosis index at 300 Hz,400 Hz and 500 Hz were(9.4±2.2)ms,(11.3±2.9)ms and(12.6±2.7)ms,respectively.Statistical analysis underscored significant variations among these measurements(300 Hz vs.400 Hz:P<0.001;300 Hz vs.500 Hz:P<0.001;400 Hz vs.500 Hz:P=0.033).Conclusion In this prospective study,myocardial T1ρ values for the specific cardiac magnetic resonance setting are provided,and we found that spin-lock frequency can affect the T1ρ values.

Purpose To explore the changes in biventricular volume and mass in chronic kidney disease(CKD)patients with preserved ejection fraction using cardiac magnetic resonance(CMR),and evaluate cardiac function using tissue-tracking strain analysis.Materials and Methods The retrospective analysis of 40 CKD patients without symptoms of cardiovascular disease,diabetes and the estimated glomerular filtration rate<60 ml/(min·1.73 m2)who were treated at the First Affiliated Hospital of Anhui Medical University from October 2020 to June 2023 and 25 age-and gender-matched healthy volunteers.All participants underwent 1.5T CMR scans to calculate left and right ventricular function,myocardial strain and native T1 and T2 values,and differences in various imaging parameters between the two groups were compared.Results The native T1 value[(1 109.6±35.9)ms]and T2 value[(58.1±2.9)ms]of CKD patients were significantly higher than those in control group[(1 046.3±15.9)ms,(53.3±2.3)ms](t=8.296,6.916,both P<0.001).The left ventricular end-diastolic volume index,left ventricular end-systolic volume index,left ventricular mass index,right ventricular end-diastolic volume index,right ventricular end-systolic volume index and right ventricular mass index of CKD patients were significantly increased compared with the control group(t=3.233,3.350,5.751,7.746,5.937,2.363,all P<0.05),while the left and right ventricular strain parameters,left ventricular global longitudinal strain,left ventricular global circumferential strain,left ventricular global radial strain,right ventricular global longitudinal strain,right ventricular global circumferential strain and right ventricular global radial strain were significantly decreased(t=9.506,9.072,-10.603,10.496,11.574,-4.018,all P<0.001),and the estimated glomerular filtration rate exhibited significant correlations with left ventricular global longitudinal strain(r=-0.636),left ventricular global radial strain(r=0.688),right ventricular global longitudinal strain(r=-0.513),right ventricular global circumferential strain(r=-0.827)and right ventricular global radial strain(r=0.514)(all P<0.001).Conclusion CMR can quantitatively assess myocardial fibrosis and edema in patients with CKD,and tissue tracking strain analysis can detect myocardial motion abnormalities before changes in ventricular ejection fraction,offering value in the early detection of cardiac involvement in CKD patients..

Objective:To analyze the evaluation of the application effect and deficiency of nurses acting as standardized parents in the graduation examination of standardized residency training of pediatrics and further improve and promote the level of standardized parents.Methods:A questionnaire survey was used to collect the scores of nurse standardized parents by students and examiners who took part in the graduation examination of standardized residency training of pediatrics in 2021. And the self-evaluation scores of standardized parents were collected. Counting data were represented by the number of cases and composition ratio. A Chi-square test was used to compare the rates.Results:A total of 125 questionnaires from students and 37 questionnaires from nurse standardized parents were collected, and the overall satisfaction (very satisfied + satisfied) of standardized parents reached 121 (96.80%). In the three dimensions of simulation ability, compliance with question-and-answer rules, and simulated attitude, students believed that the consistency between standardized parents and actual parents in simulated altitude was lower than that in the simulation ability and compliance with question-and-answer rules ( P=0.007, P=0.001). The overall satisfaction of standardized parents (very satisfied + satisfied) reached 87.38% (388/444). There were 26 (70.27%) nurse standardized parents who had the lowest satisfaction with their own performance ability, followed by 28 (75.68%) cases of imitation ability and 30 (81.08%) cases of adaptability. Conclusions:It is feasible to adopt nurse standardized parents in the assessment of standardized residency training of pediatrics, and both students and examiners have higher satisfaction. The next step is to improve the training of nurses standardized parents in the attitude of simulation and, at the same time, enhance the training of imitation ability and adaptability, so as to further expand the construction of standardized parents.

Objective To investigate the changes in the levels of serum long noncoding ribonucleic acid(ln-cRNA)growth arrest specific 5(GAS5)and tissue inhibitor of matrix metalloproteinases(TIMP)3 and the clinical significance in children with mycoplasma pneumoniae pneumonia(MPP).Methods A total of 150 children with MPP admitted to Department of Pediatrics of Yulin Xingyuan Hospital from January 2021 to October 2023 were selected as MPP group and were divided into a severe group(98 cases)and a mild group(52 cases)according to the severity of disease,and were divided into a poor prognosis group(31 cases)and a good prognosis group(119 cases)according to the 28 d prognosis.Another 60 healthy children during the same period were selected as the control group.Serum lncRNA GAS5 and TIMP3 levels were measured by re-al-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay respec-tively.Pearson correlation was used to analyze the correlation of serum lncRNA GAS5 and TIMP3 levels in children with MPP,multivariate Logistic regression was used to analyze the factors affecting the poor progno-sis in children with MPP,and receiver operating characteristic curve was used to analyze the predictive value of serum lncRNA GAS5 and TIMP3 levels for poor prognosis in children with MPP.Results Serum lncRNA GAS5 and TIMP3 levels in the severe group were lower than those in the mild group and the control group(P＜0.05),and serum lncRNA GAS5 and TIMP3 levels in the mild group were lower than those in the con-trol group(P＜0.05).Serum lncRNA GAS5 and TIMP3 levels were positively correlated in children with MPP(r=0.633,P＜0.001).The incidence of 28 d poor prognosis in 150 children with MPP was 20.67％(31/150).The proportion of severe cases,peak body temperature,procalcitonin,and C-reactive protein levels in the poor prognosis group were higher than those in the good prognosis group(P＜0.05),the length of hos-pital stay was longer than that in the good prognosis group(P＜0.05),and the levels of lncRNA GAS5 and TIMP3 were lower than those in the good prognosis group(P＜0.05).Critical illness and elevated procalcito-nin level were independent risk factors for poor prognosis in children with MPP,and elevated lncRNA GAS5 and TIMP3 levels were independent protective factors(P＜0.05).The area under the curve of serum lncRNA GAS5 combined with TIMP3 levels for predicting poor prognosis in children with MPP was 0.878,which was greater than 0.780 and 0.778 predicted by serum lncRNA GAS5 and TIMP3 levels alone(P＜0.05).Conclu-sion The decrease in serum lncRNA GAS5 and TIMP3 levels in children with MPP is closely related to the worsening of the condition and poor prognosis,the combination of serum lncRNA GAS5 and TIMP3 has high predictive value for poor prognosis in children with MPP.

Objective:To investigate the status quo of thriving at work, career resilience and voice behavior in organ transplantation nurses, and mediation effects of thriving at work between the latter two.Methods:From June to August 2018,180 nurses with organ transplant qualification departments in two Grade A hospitals in Fuzhou, Fujian Province were investigated by the general situation survey form, Thriving At Work Scale, Career Resilience Scale and Voice Behavior Scale.Results:The scores of thriving at work, career resilience and voice behavior were 3.68 ± 0.65, 3.56 ± 0.61 and 3.42 ± 0.62. There was a pairwise positive correlation among the three: thriving at work, career resilience and voice behavior( r values were 0.270-0.664, all P<0.05). Thriving at work played a partial mediating role between career resilience and voice behavior ( B values were 0.138-0.611, all P<0.05). Conclusions:Ii is necessary to improve thriving at work, career resilience and voice behavior in organ transplantation nurses. Thriving at work is the intermediary factor between the latter two. The improvement of thriving at work and career resilience will help to promote organ transplant specialist nurses to make positive voice behavior.

Hepatogenous diabetes (HD) is a common complication of end-stage liver disease, and many studies have confirmed its adverse effect on prognosis. In recent ten years, a great number of studies have been conducted on the pathogenesis of HD and some progress has been made. This article reviews the research advances in the pathogenesis of HD, in order to provide a reference for the diagnosis and treatment of HD by clinicians.

Both diabetes mellitus and liver cirrhosis have high incidence rate and mortality rate around the world, and in recent ten years, scholars in China and globally have conducted many studies on the association between diabetes mellitus and liver cirrhosis. This article systematically reviews the advances in the basic and clinical research on the influence of diabetes mellitus on liver cirrhosis and its complications and summarizes possible mechanisms. The results show that diabetes mellitus can accelerate the process of liver fibrosis, increase the risk of complications and progression to liver cancer in patients with liver cirrhosis, and reduce their survival rate.

Objective:To understand the characteristics of the HIV-1 molecular networks in injected drug users (IDUs) in Guangzhou from 2008 to 2017, and provide reference for the prevention and control of AIDS in this population.Methods:The serum samples of newly diagnosed HIV-1 positive IDUs in Guangzhou from 2008 to 2017 were collected, HIV-1 RNA was extracted for pol gene amplification and sequencing. The molecular cluster in the phylogenetic tree was identified by Cluster Picker 1.2.3 for cluster analysis. TN93 model in HyPhy2.2.4 was used to calculate the gene distance between the cluster sequences. Software Cytoscape3.8.2 was used to visualize the molecular network, and χ2 test or exact probability method was used for cluster analysis and centrality analysis. Results:A total of 586 sequences were successfully amplified (73.9%, 586/793), and 80 molecular clusters were produced, with a clustering rate of 46.6% (273/586). In molecular clusters, the proportions of the strains from IDUs in Han ethnic group (48.4%, 260/537), IDUs who were local residents in Guangdong (52.7%, 146/277) and IDUs whose strain sequence subtype was CRF55_01B (93.3%, 14/15) were higher. In the molecular network, the degree range was 1-7, and nodes with degree ≥3 accounted for 12.8% (24/187), which was associated with another 81 nodes in the molecular network (43.3%, 81/187). The centrality analysis showed that the proportions of housework/unemployed with high degree centrality (19.0%, 19/100), high intermediary (22.0%, 22/100), and high proximity centrality (32.0%, 32/100) were higher in IDUs infected with HIV-1.Conclusion:The risk of HIV-1 clustering in IDUs in Guangzhou was high, suggesting that IDUs who were from both Guangdong and Guangxi and were house workers or unemployed should be viewed as the key targets, and precise intervention should be implemented to reduce the HIV-1 infection rate in this population.