OBJECTIVE To conduct a survey of current status of surgical site infections(SSIs)among the orthope-dics department patients in the traditional Chinese hospitals of Guangdong Province so as to provide guidance for prevention and control of the SSIs.METHODS A retrospective questionnaire survey and field verification were car-ried out for the prevalence of SSIs among the orthopedics department patients of 61 secondary or above traditional Chinese hospitals(traditional Chinese medicine integrated with western medicine hospitals)in 2019.RESULTS To-tally 35 tertiary hospitals and 26 secondary hospitals were involved in the survey.A total of 167,051 patients who re-ceived orthopedic surgical procedures were investigated,355 of whom had SSIs,with the incidence 0.21％.The patients who had superficial surgical incision infections were dominant,accounting for 53.52％.The incidence rates of SSIs of grade 3 surgeries and SSIs of type Ⅰ incision were respectively 0.34％and 0.24％in the secondary hospitals,remarkably higher than those in the tertiary hospitals(all P＜0.05).There were no signficant differences in the incidence rate of SSIs of other grades of surgeries between the secondary hospitals and the tertiary hospitals.There were significant differences in the nutrition status,smoking history,complication with COPD,anemia,bath 1 night before surgery,preoperative pe-ripheral blood total white blood cell counts,type of surgery,title of surgeon,operating room,anesthesia mode,surgical site disinfectants,operation duration,American Society of Anesthesiologists(ASA)score and surgical risk index between the tertiary hospitals and the secondary hospitals(all P＜0.05).The etiological test was carried out for 331 patients with SSIs;totally 266 strains of pathogens were isolated,105(39.47％)of which were Staphylococcus aureus.CONCLUSIONS The incidence of SSIs is relatively low among the orthopedics department patients of the traditional Chi-nese hospitals(traditional Chinese medicine integrated with western medicine hospitals)in Guangdong Province.The inci-dence rates of SSIs of grade 3 surgeries and SSIs of type Ⅰ incision are remarkably higher in the secondary hospitals than in the tertiary hospitals.It is necessary to take comprehensive prevention and treatment measures according to the character-istics of the infections so as to reduce the incidence of SSIs among the orthopedics department patients.

OBJECTIVE To conduct a survey of current status of surgical site infections(SSIs)among the orthope-dics department patients in the traditional Chinese hospitals of Guangdong Province so as to provide guidance for prevention and control of the SSIs.METHODS A retrospective questionnaire survey and field verification were car-ried out for the prevalence of SSIs among the orthopedics department patients of 61 secondary or above traditional Chinese hospitals(traditional Chinese medicine integrated with western medicine hospitals)in 2019.RESULTS To-tally 35 tertiary hospitals and 26 secondary hospitals were involved in the survey.A total of 167,051 patients who re-ceived orthopedic surgical procedures were investigated,355 of whom had SSIs,with the incidence 0.21％.The patients who had superficial surgical incision infections were dominant,accounting for 53.52％.The incidence rates of SSIs of grade 3 surgeries and SSIs of type Ⅰ incision were respectively 0.34％and 0.24％in the secondary hospitals,remarkably higher than those in the tertiary hospitals(all P＜0.05).There were no signficant differences in the incidence rate of SSIs of other grades of surgeries between the secondary hospitals and the tertiary hospitals.There were significant differences in the nutrition status,smoking history,complication with COPD,anemia,bath 1 night before surgery,preoperative pe-ripheral blood total white blood cell counts,type of surgery,title of surgeon,operating room,anesthesia mode,surgical site disinfectants,operation duration,American Society of Anesthesiologists(ASA)score and surgical risk index between the tertiary hospitals and the secondary hospitals(all P＜0.05).The etiological test was carried out for 331 patients with SSIs;totally 266 strains of pathogens were isolated,105(39.47％)of which were Staphylococcus aureus.CONCLUSIONS The incidence of SSIs is relatively low among the orthopedics department patients of the traditional Chi-nese hospitals(traditional Chinese medicine integrated with western medicine hospitals)in Guangdong Province.The inci-dence rates of SSIs of grade 3 surgeries and SSIs of type Ⅰ incision are remarkably higher in the secondary hospitals than in the tertiary hospitals.It is necessary to take comprehensive prevention and treatment measures according to the character-istics of the infections so as to reduce the incidence of SSIs among the orthopedics department patients.

Objective:To explore the characteristics and influencing factors of blood carnitine metabolism in premature infants.Methods:A retrospective analysis of 37 037 neonates with negative results of genetic metabolic disease screening at Guangxi Newborn Disease Screening Center from 2018 to 2021, of which 34 517 normal full-term infants were the control group and 2 520 preterm infants were the research group.According to gestational age, the preterm infants were further divided into three groups: extremely preterm group( n=232), moderately preterm group( n=324)and late preterm group( n=1 964). According to birth weight, they were divided into three groups: very low birth weight group( n=188), low birth weight group( n=1 276)and normal birth weight group( n=1 056). According to blood collection time, they were divided into three groups: 3~7 days group( n=1 990), 8~14 days group( n=342) and 15~28 days group( n=188). Tandem mass spectrometry was used to detect the levels of 31 carnitines in dried blood spots and analyze the differences in the levels of metabolic indicators in each group. Results:Carnitine levels in preterm infants are most affected by gestational age.Adjusting the physiological and pathological conditions of premature infants and other related factors, grouped by gestational age, there were differences in the levels of 31 carnitines among the groups(all P<0.05), the smaller the gestational age, the greater the difference in carnitine levels; grouped by blood collection time, there were statistically significant differences in carnitine levels between preterm infants with different blood collection age groups and full-term 3~7 days groups(all P<0.05), and showing age-related; there are differences among 31 carnitines grouped by body weight(all P<0.05), the smaller the body weight, the greater the difference in carnitine levels.Combined with the analysis of gestational age, birth weight and blood collection date, 17 indicators including C0, C2, C3, C4, C6DC, C10, C10∶1, C12, C12∶1, C14, C14∶1, C14OH, C16, C16∶1, C18, C18∶1 and C18∶1OH are important biomarkers of carnitine metabolism in premature infants. Conclusion:Carnitine in premature newborns has different metabolic differences at different gestational ages, birth weights and blood collection ages, which provides a strong basis for establishing reference standards and interpretation of preterm infants in the laboratory in this region, and provides reasonable and effective early diagnosis and treatment for clinical practice.Meanwhile, it provides an optimized program for timely detection of carnitine deficiency and carnitine supplementation to improve nutrition of premature infants.

OBJECTIVE: To analyze the metabolic profile and genetic variants for newborns with primary carnitine deficiency (PCD) from Guangxi, China. METHODS: From January 2014 to December 2019, 400 575 newborns from the jurisdiction of Guangxi Zhuang Autonomous Region Newborn Screening Center were subjected to tandem mass spectrometry (MS/MS) analysis. Newborns with positive results for PCD and their mothers were recalled for retesting. Those who were still positive were subjected to sequencing of the SLC22A5 gene. RESULTS: Twenty-two newborns and 9 mothers were diagnosed with PCD, which gave a prevalence rate of 1/18 208. Sequencing of 18 newborns and 4 mothers have identified 14 types of SLC22A5 gene variants, with the common ones including c.51C>G (10/44, 22.7%), c.1195C>T (9/44, 20.5%) and c.1400C>G (7/44, 15.9%), The c.517delC(p.L173Cfs*3) and c.1031C>T(p.T344I) were unreported previously and predicted to be pathogenic (PVS1+PM2_supporting+PM3+PP4) and likely pathogenic (PM1+PM2_supporting+PM3+PP3+PP4) based on the American College of Medical Genetics and Genomics standards and guidelines. CONCLUSION c.51C>G, c.1195C>T and c.1400C>G are the most common variants underlying PCD in Guangxi.
Cardiomyopathies ; Carnitine/deficiency* ; China ; Humans ; Hyperammonemia ; Infant, Newborn ; Metabolome ; Muscular Diseases ; Mutation ; Solute Carrier Family 22 Member 5/genetics* ; Tandem Mass Spectrometry

Objective To develop a HPLC method for determining the contents of lobetyolin and gallic acid in Eighteen Flavors Dangshen Pill(EFDSP) produced by different factories.Methods The HPLC analysis was performed on a VP-DOS C18 column (4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile and 0.4% glacial acetic acid(21∶79) in the determination of lobetyolin content.The detection wavelength was 267 nm and the flow velocity was 1 mL/min.the column temperature was25 ℃ and the sample size was10 μL.The mobile phase was methanol and 0.4% glacial acetic acid(1∶99) in the determination of gallic acid content.The detection wavelength was 280 nm.The column temperature was 25 ℃ and the sample size was 10μL.Results The contents of lobetyolin and gallic acid in EFDSP were 1.0835mg·g-1and 15.334 0 mg/g for Qinghai Gela Dandong Tibetan Pharmaceutical Factory;0.628 9 mg/g and 15.159 5 mg/g for Changdu Tibet Medicine Factory;0.306 5 mg/g and 8.762 7 mg/g for Tibetan Hospital of Tibet.Conclusion This method has the advantages of good reproducibility,good accuracy,simple and fast operation.The contents of lobetyolin and gallic acid in EFDSP produced by different manufacturers are significantly different.The gallic acid content has greater difference.It provides the reference for quality control of EFDSP

Objective To use the matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) technique for detecting the mutation gene of neonatal non-syndromic hereditary hearing impairment gene in Guangxi and to investigate its effectiveness and feasibility in clinical application.Methods A total of 7 100 newborns were performed the hearing preliminary screening and secondary screening by adopting AABR.The genomic DNA was extracted by the heel blood spot.Twenty mutation characteristics of 4 deaf predisposing genes were detected by MALDI-TOF-MS.Results The pass rate of hearing screening in 7 100 newborns was 97.11％ (6 895/7 100),the positive rate of neonatal gene mutation was 3.54％ (251/7 100),in which the GJB2 gene mutation was in 131 cases,the carrying rate was 1.84％,235delC heterozygous mutation was in 108 cases.SLC26A4 gene mutation was in 93 cases,which dominated by 1229C＞T heterozygous mutation and IVS7-2A＞G heterozygous mutation,mtDNA12SRNA gene mutation was in 16 cases and GJB3 gene mutation was in 11 cases.Conclusion Adopting the MALDI-TOF-MS screening technique can increase the detection rate of hot point mutation in common deaf related genes and discover neonatal genetic NSHI from molecular level and provides the corresponding geneticconsulting guidance for early finding and predicting deaf occurrence,and formulating the interventional measures.

AIM: To investigate the relationship among MCP-1 and monocyte chemoattract protein activity (MCA) and pathogenesis of lung cancer. METHODS: 173 patients were involved in the study and divided into three groups: group A: lung cancer group (60 patients); group B: benign lung disease group (55 patients) and group C: healthy control group (58 patients). MCP-1 level and MCA in bronchoalveolar lavage fluid (BALF) were measured. RESULTS: The concentration of MCP-1 and MCA in BALF in group A were much higher than those in group B and group C (P