Objective To explore the value of serum family with sequence similarity 19 member A5(FAM19A5)and vitronectin in evaluating the degree of coronary artery disease and major adverse cardiovas-cular events(MACE)in patients with coronary heart disease(CHD)complicated with heart failure(HF).Methods From March 2022 to March 2023,120 patients with CHD complicated with HF in this hospital were collected as the study group,and 76 healthy individuals who underwent physical examination in the hospital were as the control group.Patients in the study group were divided into mild group(41 cases),moderate group(59 cases)and severe group(20 cases)according to Gensini score and degree of coronary artery dis-ease.Meanwhile,they were divided into MACE group(36 cases)and non-MACE group(84 cases)according to whether MACE occurred 6 months after treatment.Serum FAM19A5 and vitronectin levels were detected by enzyme-linked immunosorbent assay(ELISA),and the correlation between serum FAM19A5 and vitronec-tin levels and Gensini score in CHD patients with HF was analyzed by Pearson method.Multivariate Logistic regression was used to analyze the influencing factors of MACE in CHD patients with HF,and receiver operat-ing characteristic(ROC)curve was used to evaluate the predictive value of FAM19A5 and vitronectin for MACE in CHD patients with HF.Results The serum FAM19A5 level in the study group was significantly decreased(P＜0.05),and the serum vitronectin level was significantly increased(P＜0.05).The serum FAM19A5 level in severe group was significantly lower than that in mild and moderate groups(P＜0.05),and the serum vitronectin level was significantly higher than that in mild and moderate groups(P＜0.05).The serum FAM19A5 level in MACE group was significantly lower than that in non-MACE group(P＜0.05),and the serum vitronectin level was significantly higher than that in no-MACE group(P＜0.05).Ser-um FAM19A5 level in CHD patients with HF was negatively correlated with Gensini scores(r=-0.755,P＜0.001),and vitronectin level was positively correlated with Gensini scores(r=0.697,P＜0.001).The risk factors for MACE in CHD patients with HF were cardiac function grade Ⅲ-Ⅳ,decreased FAM19A5 lev-el,increased LDL-C,vitronectin level and Gensini scores(P＜0.05).The area under the curve(AUC)of ser-um FAM19A5,vitronectin and the combination of FAM19A5 and Vitronectin were 0.882,0.877,and 0.962,respectively,which were superior to the AUC of FAM19A5 and Vitronectin alone(Z=2.612,2.188,P=0.009,0.029).Conclusion The serum levels of FAM19A5 and vitronectin are significantly correlated with the degree of coronary artery disease in CHD patients with HF,and the combination of Fam19A5 and Vitronectin has a high predictive value for the occurrence of MACE.

Objective To investigate whether polypeptide cSN50,as a transmembrane peptides,can inhibit NF-κB nuclear translocation and its downstream gene expression to play a role to protect cells by blocking the combination of NF-κB with the importinα3 during the alcohol and endotoxin-induced inflammation and apoptosis.Methods Flow cytometry method was used to observe the apoptosis rate of HepG2 by different concentration of alcohol and/or endotoxin.Spectrophotometer method was used to detect Caspase-3 activity.TNF- α in cell culture supernatant was detected by ELISA assay.p50,importinα3,and IκBαunder the stimulation of alcohol and/or endotoxin at selected optimal concentration were detected by Western blot.Indirect immunofluorescence assay was used to measure the activation of p50,IκBα,and importinα3.Results The change of TNF-α and Caspase-3 in the dose-effect course,compared with control group,was significant (P ＜0.05 ).p50 was increased in the nucleus ( P ＜ 0.05 ),and IκBα was decreased in the cytoplasm ( P ＜ 0.05 ).cSN50( 100 μmol/L) partially blocked nuclear translocation of p50 and its downstream factor( P ＜ 0.05 ).Conclusion NF-κB nuclear translocation may play an important role in acute alcoholic liver injury.cSN50 can effectively inhibit NF-κB activity and its downstream gene expression in HepG2 cells.