The zebrafish is characterized by its rapid reproductive cycle,transparent embryonic body in the early developmental stages,high genetic and physiological homology with humans,and low breeding costs.These advantages make it a valuable tool that complements mammalian models of depression.The zebrafish has emerged as a promising model organism for studying mental illnesses owing to its unique biological characteristics and the diverse method available for establishing depression models using this species.This article aims to provide a comprehensive review of zebrafish depression model establishment method,evaluation criteria,advantages,and limitations.

The zebrafish is characterized by its rapid reproductive cycle,transparent embryonic body in the early developmental stages,high genetic and physiological homology with humans,and low breeding costs.These advantages make it a valuable tool that complements mammalian models of depression.The zebrafish has emerged as a promising model organism for studying mental illnesses owing to its unique biological characteristics and the diverse method available for establishing depression models using this species.This article aims to provide a comprehensive review of zebrafish depression model establishment method,evaluation criteria,advantages,and limitations.

Objective: To evaluate the safety and efficacy of enhanced recovery after surgery (ERAS) in robot-assisted laparoscopic pyeloplasty in pediatric patients. Methods: Sixty pediatric patients of both sexes with hydronephrosis, aged 3-12 yr, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, undergoing robot-assisted laparoscopic pyeloplasty from March 2018 to April 2019, were divided into 2 groups using a random number table method: control group (group C, n=28) and ERAS group (n=32). In ERAS group, preoperative ERAS education was carried out, the time of preoperative food and water deprivation was shortened, pediatric patients drank glucose water at 2 h before surgery, anesthetic regimen was optimized, lung protective ventilation and target-directed fluid therapy were performed, and intraoperative warming and multi-mode antiemetic measures were carried out during operation, and multi-mode analgesic measures were taken after operation, and pediatric patients received water and food intake early through the mouth and got out of bed as soon as possible after operation.In group C, the traditional concept was adopted for perioperative management.Immediately after tracheal intubation, at 30 min and 1 and 2 h after establishing pneumoperitoneum, at 5 min after the end of pneumoperitoneum and at 5 min after extubation, the airway peak pressure and tidal volume were recorded, and blood gas analysis was performed.The occurrence of cardiovascular events was recorded during surgery.The postoperative time of extubation, time of first intake, the first postoperative off-bed time, the first flatus time, time of pulling out the ureter and drainage tube, and length of hospital stay were recorded.The Pediatric Anesthesia Emergence Delirium scale was used to assess the agitation during the recovery period.The Faces Pain Scale-Revised scale was used to assess the degree of pain within 72 h after surgery.When Faces Pain Scale-Revised scale score ≥4, fentanyl 0.25 μg/kg was intravenously injected as rescue analgesic.The requirement for rescue analgesia was recorded.The overall complications were evaluated by using Clavin-Dindo grading, and postoperative complications included nausea and vomiting, abdominal distension, abdominal pain, incision infection, abdominal infection, anastomotic leakage, fever, etc. Results: Compared with group C, the preoperative food and water deprivation time was significantly shortened, the time of postoperative extubation was prolonged, the postoperative length of hospital stay, time of first intake, the first postoperative off-bed time, the first flatus time, and time of pulling out the ureter were shortened, airway peak pressure was decreased at 1 and 2 h of pneumoperitoneum, arterial blood lactate concentrations were decreased at each time point of pneumoperitoneum (P<0.05 or 0.01), and no significant change was found in the incidence of postoperative agitation, nausea and vomiting, incision infection, abdominal infection or fever in group ERAS (P>0.05). No intraoperative adverse cardiovascular events were found, and no pediatric patients required rescue analgesia after operation in two groups. Conclusion ERAS can be safely and effectively used for the pediatric patients undergoing robot-assisted laparoscopic pyeloplast.

Objective To explore the clinical effects of the combination of the self-made negative pressure drainage and Nano-Silver dressings on postoperative infection of incision.Methods A total of 84 patients with post-operative incision infection in the First People's Hospital of Xiangtan City from January 2013 to December 2015 were selected and divided into the observation group and the control group with 42 cases in each by random number table method.The observation group was treated with self-made negative pressure device combined with Nano-Silver dressing at the early stage,while the control group was treated with iodoform gauze dressing.When the wound bed granulation grew well,the two groups were treated with calcium alginate dressing or two stage suture until healed.The inflammatory index,incision growth and therapeutic effect of the two groups were compared.Results A week after treatment,the white blood cell,C reactive protein,incisional pain score and the change cost of the observation group were (7.62 ± 2.15) × 109/L,(19.33 ± 23.46) mg/L,(2.83 ± 0.82) and (570.13 ± 33.88) Yuan,which were all lower than those of the control group,and the differences were statistically significant (t=2.592,2.695,7.497,7.752;P ＜ 0.05).100％ coverage time of the incisional granulation tissue in the observation group was (3.86 ± 1.37) d,and the healing time of the whole incision was (12.02 ± 2.80) d in the observation group,which were both lower than those in the control group,with statistical significance (t=12.669,6.954;P ＜ 0.01).Conclusions The self-made negative pressure device combined with Nano-Silver dressing is ideal for the treatment of postoperative incision infection,while improving the therapeutic effect of the patients,and reducing the healing time of the incision and the cost of changing the medicine.

Objective To explore the effect of social medical treatment in improving the quality of life for patients with nasopharyngeal carcinoma.Methods 32 patients with nasopharyngeal carcinoma were mentioned with social medical treatment (including psychological treatment,family visit,family tend,social healing service,etc).The result was measured by using the SQOL-NPC.and the different stages of the results were compared.Results All the patients were tracked in 2 years.All the scores were mark down in a table.Conclusion After analyzing the results,it was found that social medical treatment played an important role in improving the quality of life for patients with nasopharyngeal carcinoma.