ObjectiveTo explore the effects of electroacupuncture at governor vessel or Jiaji acupoints on the activation of lumbar spinal cord neurons and the recovery of motor function in paralyzed hind limbs in rats with complete transection of spinal cord injury. MethodsTwenty adult female SD rats were selected. Among them， 15 rats underwent the complete transection of the 10th thoracic spinal cord and were randomly divided into the Control group， the electroacupuncture at governor vessel acupoints （GV） group and the electroacupuncture at Jiaji acupoints （JJ） group， with 5 rats in each group. The other 5 rats served as the Normal group. Electroacupuncture treatment was initiated 3 days after surgery in the GV and JJ groups and performed every other day. Basso-Beatti-Bresnahan （BBB） behavioral score was evaluated weekly. Eight weeks after the operation， cortical motor evoked potential （CMEP） detection was performed， followed by perfusion fixation， tissue harvest， frozen sectioning， and immunofluorescence staining experiments. Results① Both electroacupuncture treatments activated the expression of the excitatory marker c-Fos in L1 spinal cord neurons. The c-Fos⁺ activated neurons were mainly distributed in lamina Ⅳ， Ⅴ and Ⅶ of the L1 spinal cord， while no c-Fos⁺ neuron was observed in the Normal group and the Control group. The percentages of c-Fos⁺ neurons activated by electroacupuncture in the GV group and the JJ group were （10.23±0.15） % and （10.77±1.12） %， respectively， and there was no significant difference between the two electroacupuncture groups. ② vGluT1⁺ proprioceptive afferent nerve fibers were mainly distributed in the dorsal horn and lamina Ⅶ of intermediate zone of L1 spinal cord gray matter. In the dorsal horn of L1 spinal cord， the relative fluorescence intensity of vGluT1⁺ nerve fibers in the GV group and the JJ group were greater than that in the Control group， and that in the JJ group was greater than that in the GV group. In the lamina Ⅶ in intermediate zone of L1 spinal cord， where the central pattern generator （CPG） for hindlimb rhythmic movement is located， the relative fluorescence intensity of vGluT1⁺ nerve fibers in the GV group and the JJ group were significantly higher than that in the Control group， and the JJ group was also higher than the GV group； The lamina Ⅶ neurons innervated by vGluT1⁺ axon terminals were activated to express the excitatory marker c-Fos in the GV group and the JJ group. ③ The immunofluorescence staining results of acetylcholinesterase （ChAT） in the ventral horn of L4 spinal cord of each group showed that the number of surviving ChAT⁺ motor neurons in the GV group （19.1±3.4， P=0.000 2） and JJ group （19.0±2.9， P=0.000 2） were higher than that in the Control group （9.9±1.9）. ④ The quantitative analysis of the vGluT2⁺ excitatory axonal terminals， vGAT⁺ inhibitory axonal terminals， and the ratio of vGluT2⁺/vGAT⁺ axonal terminals ［reflecting the excitation/inhibition （E/I） balance of motor neurons］ innervating ChAT⁺ motor neurons in the ventral horn of the L4 spinal cord revealed that all three indices were significantly higher in the GV and JJ groups than in the Control group. Moreover， the E/I ratio of motor neurons in the two electroacupuncture groups gradually reached the same level as normal and showed no statistical difference from the Normal group. ⑤ The electrophysiological and behavioral detection results showed that compared with the Control group， the latencies of CMEP in the GV group and the JJ group shortened， while their amplitudes increased. The BBB score of paralyzed hindlimbs showed that after 8 weeks of treatment， the scores of both the GV group （4.25±1.26， P=0.023 4） and the JJ group （4.75±0.96， P=0.000 2） were higher than those of the Control group （1.80±1.30）， but there was no statistical difference between the two electroacupuncture groups. ConclusionElectroacupuncture at GV or JJ acupoints may promote the afferent of vGluT1⁺ proprioceptive nerve fibers into the spinal cord， indicating the possibility to activate CPG neurons in lamina Ⅶ of L1 spinal cord； facilitate the survival of motor neurons in the L4 spinal cord and restore their excitatory/inhibitory balance， improving the motor function of the paralyzed hindlimbs in rats.

Objective To study the inhibitory effect and related mechanisms of Qiangxin Capsule on fi-brosis caused by Wnt(wingless)/β-catenin signaling pathway in Sprague Dawley(SD)heart failure(HF)rats.Methods Fifteen SPF-grade SD male rats aged 6-8 weeks were selected as the research subjects.The rats were randomly divided into the sham operation group,model group and medication group,5 cases in each group.The model group and medication group caused abdominal aorta partial stenosis by operation.The rats in the sham operation group and model group were gavaged by 10 mL/kg of normal saline,the rats in the medication group were gavaged by 0.8 g/kg Qiangxin Capsule,and each group was continuously administered for 20 weeks.The ventricular wall motion amplitude was used to detect the left ventricular ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVEDs)and left ventricular end-diastolic diameter(LVEDd)in each group.After 20 weeks,the rats in each group were executed,the rat myocardial tissues were taken for conducting the pathological analysis,the myocardial tissues were observed by hematoxylin-eosin(HE)staining,the fibrous tissues were observed by the Masson staining,and the protein expression levels of Wnt,β-catenin,collagen Ⅰ and collagen Ⅲ were detected by Western blot.Results LVEF in the medication group was higher than that in the model group,LVEDd and LVEDs were smaller than those in the model group,and the differences were statistically significant(P＜0.05).Compared with the model group,the Wnt/GAPDH and β-catenin/GAPDH levels in the medication group were decreased,and the difference was statisti-cally significant(P＜0.05).Compared with the model group,the collagen Ⅰ/GAPDH and collagen Ⅲ/GAP-DH in the medication group were decreased,and the difference was statistically significant(P＜0.05).Conclu-sion Qiangxin Capsule could alleviate the cardiac fibrosis degree in SD rats with heart failure after abdominal aortal coarctation,and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.

OBJECTIVE To evaluate the efficacy and safety of dydrogesterone in the treatment of dysmenorrhea. METHODS The prospective ，random-controlled，open-labeland multicenter clinical study was adopted. A total of 108 women with dysmenorrhea were randomly assigned into dydrogesterone group and control group according to the ratio of 1∶1，with 54 patients in each group. Dydrogesterone group was treated with dydrogesterone 10 mg orally ，twice a day ，on the 5th-25th day of menstrual cycle ，for 3 menstrual cycles. Control group received Guizhi fuling capsule 0.93 g orally ，three times a day，since the end of menstrual bleeding to the third day of the next menstruation ，for 3 menstrual cycles. Main results were the changes of visual analogue scale （VAS）scores in 2 groups after 3 menstrual cycles ；secondary results were the changes of COX menstrual symptom scale （CMSS），quality life of 36-item short form （SF-36），levels of carbohydrate antigen 125（CA125）and interleukin 6（IL-6）after 3 menstrual cycles ；other findings included additional benefits and drug safety. RESULTS The results of intention to analysis data set and the follow-up study protocol analysis data set showed that VAS scores of 2 groups after treatment of dysmenorrhea for 1，2 and 3 menstrual cycles were lower than those before treatment ，the longer the treatment time ，the more obvious the decrease of VAS score （P＜0.05），and VAS score decline of dydrogesterone group was better than that of control group（P＜0.05）. After 3 menstrual cycles ，both the two group showed significant reduction in the severity and duration scores of CMSS（P＜0.05）；and the decrease of the above scores in the dydrogesterone group was superior than in the control group （P＜ 0.05）. After 3 menstrual cycles ，among 8 dimensions of SF- 36 scale，the scores of 7 dimensions in dydrogesterone group were significantly higher than those before treatment ，such as the scores of physiological function ，physical role ，physical pain ， emotional function ，social function ，general health status and energy （P＜0.05）；the increase of the scores of four dimensions were higher than those in the control group ，such as physical pain ，social function ，general health status ，energy（P＜0.05）. There was no significant difference in the levels of CA 125 and IL- 6 between 2 groups before and after treatment （P＞0.05）. After 3 menstrual cycles，the menstrual cycle and menstrual period in the dydrogesterone group were shorter than those before treatment ，and the menstrual volume decreased （P＜0.05）；but there was no significant change in the above indexes of control group （P＞0.05）. After 3 menstrual cycles ，the incidence of adverse drug events and adverse reactions in dydrogesterone group was 32.69％（17/52）and 28.85％（15/52）；no serious adverse drug events or adverse reactions such as thrombosis occurred in both groups. CONCLUSIONS Dydrogesterone can effectively reduce the VAS score ，also relieve dysmenorrhea-related symptoms ，and improve the quality of life. The efficacy of dydrogesterone is superior than that of Guizhi fuling capsule in treatment for dysmenorrheal ，without serious adverse reactions. It is well tolerated.

Objective:To explore the clinical effect of Huayu-Ningkun liquid enema and external application of dreg for treating chronic pelvic inflammatory disease. Methods:A total of 106 patients with chronic pelvic inflammatory disease who met the inclusion criteria in the gynecological outpatient department of Beijing Hospital of Integrated Chinese and Western Medicine from January 2018 to December 2019 were divided into 2 groups by random number table method, with 53 patients in each group. The control group was treated with conventional western medicine therapy, and the observation group was combined with Huayu-Ningkun liquid enema and external application of drug residue on the basis of the control group. TCM symptom scores were performed before and after treatment, and the Quality of Life of patients was evaluated by Generic Quality of Life Inventory-74 (GQOLI-74). Serum CRP and TNF-α levels were detected by ELISA, and adverse reactions during treatment were recorded. Results:The total effective rate was 96.2% (51/53) in the observation group and 83.0% (44/53) in the control group, and the difference between the two groups was statistically significant ( χ2=4.970, P=0.026). After treatment, the TCM symptom scores of the observation group was significantly lower than that of the control group ( t=8.205, P<0.01). The scores of physical function, mental function, material life and social function were significantly higher than those in the control group ( t value were 7.503, 8.786, 7.798, 9.353, all Ps<0.001). After treatment, the levels of serum CRP (4.52 ± 3.46 g/L vs. 8.23 ± 3.35 g/L, t=5.608) and TNF-α (1.00 ± 0.35 μg/L vs. 1.52 ± 0.28 μg/L, t=14.551) in the observation group were significantly lower than those in the control group ( P<0.01). Conclusion:The Huayu-Ningkun liquid enema and drug residue external application combined with western medicine can relieve the symptoms, reduce the level of inflammatory cytokines, improve clinical efficacy of the patients with chronic pelvic inflammatory disease.

Objective:To assess the changes of left ventricular systolic function and global synchronization and myocardial work in patients with left bundle branch pacing (LBBP) by two-dimensional multi-layered speckle tracking imaging.Methods:Forty-two patients with Ⅱ degree Ⅱ type or Ⅲ degree atrioventricular block (AVB) in the Cardiovascular Hospital of Xiamen University from April to December 2019 were selected as pacing group, which were further divided into two groups according to different pacemaker modes: twenty patients with right ventricular septal pacing (RVSP), twenty-two patients with LBBP, and twenty patients with normal ECG and cardiac structure were enrolled as control group. Echocardiography of pacing group and control group was performed and analyzed. The left ventricular subendocardial longitudinal strain peak(LSendo), the middle layer myocardial longitudinal strain peak(LSmid), subepicardial longitudinal strain peak(LSepi), global myocardial longitudinal strain peak(GLS), peak strain dispersion (PSD), global work index (GWI), global constructive work (GCW), global wasted work (GWW) and global work efficiency (GWE) were acquired. The differences among the three groups were analyzed and the high difference indexes were screened by statistical modeling.Results:LSendo in three groups had no difference ( P>0.05). LSmid, LSepi, GLS in the control group, LBBP group and RVSP groups were decreased gradually ( P<0.05). GLS in LBBP group was higher than in RVSP group ( P<0.05). Compared with the control group, the increases of QRS and PSD in LBBP group were not statistically significant ( P>0.05), while the decreases of QRS and PSD in LBBP group were statistically significant compared with the RVSP group ( P<0.05). The values of GCW among three groups had no statistical significance ( P>0.05). Compared with the control group, the decrease of GWI in LBBP group was not statistically significant ( P>0.05), while the increase of GWW and the decrease of GWE were statistically significant ( P<0.05). Compared with the RVSP group, the increases of GWI and GWE and the decrease of GWW in LBBP group were statistically significant ( P<0.05). QRS, LSendo, GLS, LSmid, left ventricular apex rotation to basal rotation peak time(ApexBase period) were the indexes with significant difference among LBBP and RVSP groups and all index characteristics showed better in LBBP than RVSP group. Conclusions:Two-dimensional multi-layered speckle tracking imaging can be used to evaluate the effect of LBBP on left ventricular systolic function and global synchronization and myocardial work. LBBP longitudinal mechanical synchronization is better than right ventricular septal pacing by improving the peak global myocardial longitudinal strain and myocardial work after pacemaker.

Objective To evaluate the effects of nitrous oxide inhalation on conscious sedation of patients during colonoscopy and analyze its risk factors. Methods From October 2016 to July 2017, a total of 154 patients undergoing colonoscopy were included in the study. The comfort level was rated using Modified Gloucester Comfort Scale ( MGCS). Factors affecting comfort level were analyzed by univariate analysis and multivariate Logistic regression, and then a comfort stratification predicting model was created. Results All 154 patients finished colonoscopy. The mean time of ideal anesthesia state was 195. 15 s. Among the 154 cases, 115 ( 74. 7%) were identified as comfort ( grade of MGCS from 1 to 3) and 39 (25. 3%) were rated as discomfort ( grade of MGCS from 4 to 5). Multivariate regression indicated that age ≥60 years ( P＝0. 000, OR＝1. 074, 95%CI: 1. 036-1. 114) and mild anxiety ( P＝0. 018, OR＝3. 338, 95%CI: 1. 227-9. 079) were associated with comfort level during colonoscopy.The established model with the age (X1) and mild anxiety (X2) was P＝eY/(1+eY), Y＝－3. 812+0. 071X1+1. 205X2(no anxiety was assigned 1, mild anxiety was assigned 2), and the area under the receiver operating characteristics curve was 0. 746 (95%CI: 0. 661-0. 830), which showed moderate predictive power of the final model. The model reached the highest predictive accuracy when the Yoden Index was 0. 838 with sensitivity of 50. 4% and specificity of 89. 7% for predicting comfort level of patients in conscious sedation status induced by nitrous oxide inhalation during colonoscopy. Conclusion Nitrous oxide can ease pain during colonoscopy, especially for patients over 60 years old and with anxiety. We can use it as an option according to actual circumstance in practice.

Objective To investigate the clinical efficacy and safety of six-hole moxibustion box therapy for stable chronic obstructive pulmonary diseases(COPD).Methods Sixty patients with thoracic facet joint disorder were randomly allocated to treatment and control groups, 30 cases each. The control group inhaled tiotropium bromide inhalation powder spray and the treatment group received six-hole moxibustion box therapy in addition. The COPD Assessment Test (CAT) score and the dyspnea score were recorded and peripheral blood oxygen saturation was measured in the two groups before and after treatment. The adverse reactions were monitored in the two groups. Results There were statistically significant pre-/post-treatment differences in the CAT score and the dyspnea score in the two groups (P<0.05). There were statistically significant post-treatment differences in the CAT score and the dyspnea score between the treatment and control groups (P<0.05). There were statistically significant differences in pre-/post- treatment CAT score difference value and dyspnea score difference value between the two groups (P<0.05). There was a statistically significant pre-/post-treatment difference in peripheral blood oxygen saturation in the treatment group (P<0.05). There was a statistically significant post-treatment difference in peripheral blood oxygen saturation between the two groups (P<0.05).Conclusion Six-hole moxibustion box therapy plus tiotropium bromide inhalation powder spray is safe and effective in treating stable COPD.

Objective:To find out the prevalence and risk factors of Atopic Dermatitis(AD) of children under 1 year old in Shihua area ,Jinshan District ,Shanghal .Methods:Neonatal general conditions at birth were collected in Jinshan Hospital , Fudan University from September 2012 to August 2013 ,while questionnalre survey was conducted to understand the correlative factors .Follow‐up was done at 1 ,2 ,6 ,9 ,12 month‐old so as to understand the incidence of AD and the conditions about feeding and growth .Results:There were 2426 newborns were selected ,and 915 were followed‐up .There were 271 AD patients ,and the prevalence rate is 29 .6% .Prevalence rate of AD was 29 .9%‐37 .6% in newborns who were born in March , April ,May ,July ,August and September ,which was higher than those in other months(20 .7%‐24 .2% ) .Univariate analysis showed that the prevalence of AD was significantly correlated with parents’ allergies and increase of the eosinophil count in infant blood (P<0 .01) .It was also correlated with boy ,caesarean section ,artificial feeding or mixed feeding ,mother’s high level of education of mothers ,passive smoking ,and there were significant differences (P< 0 .05) .However ,it was not correlated with the mother’s prenatal history of infection (P>0 .05) .Multivariate logistic regression analysis showed that AD prevalence rate was significantly correlated to mother’s high level of education ,increase of eosinophil count in infant blood(P<0 .01) .It was also correlated to caesarean section ,artificial feeding or mixed feeding ,parental allergy history ,and there were significant differences(P< 0 .05) .Conclusions:The prevalence rate of infant AD in Jinshan District of Shanghal City was 29 .6% .The incidence of AD had seasonal trends .Infants ,who were born in the spring and summer ,had higher prevalence rates of AD .Parents with allergies ,mother’s high level of education ,artificial feeding or mixed feeding after birth ,caesarean section and increase of eosinophil count in infant blood are risk factors for infant AD .