Background/Aims: Cholangiocarcinoma (CCA) is a primary malignant neoplasm with an extremely poor prognosis. While combined chemoradiotherapy has been demonstrated to delay CCA progression to a certain extent, the absence of specific molecular biomarkers or targets significantly hinders the diagnosis and treatment of CCA. Methods: Through cross-analysis of proteomics and ADMA modificationomics, we identified DDX1 overexpressed in CCA with elevated R602-ADMA modifications. HPLC-MS/MS identified PRMT1 as the methyltransferase and USP10 as the deubiquitinating enzyme for DDX1. Immunofluorescence and nuclear-cytoplasmic partitioning experiments confirmed DDX1’s nuclear localization. GO and KEGG analyses clarify the biological functions of DDX1 in response to hypoxia. RNA-seq transcriptomics analyzed key pathways influenced by DDX1. A hydrodynamic in situ CCA mouse model was established to validate the chemopreventive effects of the PRMT1-specific inhibitor GSK715 on CCA development. Results: DDX1 promotes CCA progression both in vivo and in vitro and can be inhibited by GSK715. Mechanistically, PRMT1 mediates ADMA modification at position R602 of DDX1. This modification promotes DDX1 nuclear localization by recruiting USP10 to deubiquitinate DDX1, while simultaneously inhibiting PRMT1 degradation. DDX1 promotes the transcription of PRMT1 and USP10 by binding to the mRNA 3’UTR region, establishing a positive feedback regulatory pathway. This mechanism promotes the occurrence and development of CCA and can serve as a target for the inhibitor GSK715 to suppress CCA progression. Conclusions Our study identified DDX1-R602-ADMA modification as a novel ADMA modification in CCA. It further confirmed its pivotal role in CCA progression. Targeting the USP10-PRMT1-DDX1 axis may represent a significant therapeutic approach for CCA.

Objective:To investigate the effects of calf spleen extract(CSE)on immune function,serum ferritin levels,and adverse reactions in patients with esophageal squamous cell carcinoma undergoing adjuvant chemotherapy after surgery,and to verify its effects on esophageal cancer cell proliferation and apoptosis in vitro.Methods:A total of 96 postoperative chemotherapy patients with esophageal squamous cell carcinoma at Tianjin Cancer Hospital Airport Hospital were selected and randomly divided into control group and treatment group.They were treated with TP regimen as routine,and then treated with CSE in the treatment group.After the treatment was completed,adverse reactions and clinical reactions were observed in the two groups,and immune related indicators and ferritin levels were compared before and after treatment.Compared the disease free survival(DFS)differences between two groups of pa-tients during a follow-up period of 1 and 3 years.Using MTT assay to determine the IC50 value of CSE in esophageal cancer cells.Plate cloning method was used to detect the changes in the proliferation ability of esophageal squamous cell carcinoma cells induced by CSE.Comet assay detected DNA strand damage in esophageal cancer cells caused by CSE.Flow cytometry was used to detect the effect of CSE on apoptosis of esophageal cancer cells.Results:Comparison between the two groups of patients before and after treatment showed that CSE had a significant effect on CD3+T,CD4+T,CD8+T,CD4+T/CD8+T,Iron protein levels had an impact.CSE could alleviate the adverse reactions of postoperative chemotherapy in patients with esophageal cancer,and improve their quality of life and clinical symp-toms.CSE treatment could improve short-term DFS in postoperative patients with esophageal cancer.The IC50 concentration of CSE in esophageal cancer cells was 3.018 mg/ml;CSE could inhibit the proliferation of esophageal cancer cells and increase DNA strand dam-age in esophageal cancer cells.CSE can increase apoptosis of esophageal cancer cells.Conclusion:CSE can inhibit the proliferation of esophageal cancer cells,promote their apoptosis,and improve the quality of life and short-term DFS of esophageal cancer patients after treatment by regulating immune function and reducing adverse reactions.

Objective:To investigate the effects of moxibustion at Tianshu(ST25)acupoint on 5-fluorouracil(5-FU)-induced intestinal mucositis(IM)and its underlying mechanisms.Methods:Eighteen C57BL/6 male mice were randomly divided into four groups:normal control(NC),IM model(IM),moxibustion 15 min(MO 15 min),and moxibustion 30 min(MO 30 min).The IM model was established via intraperitoneal injection of 5-FU.Pathological changes in colon tissues were observed using hematoxylin and eosin(HE)staining.Protein expression levels of peroxisome proliferator-activated receptor alpha(PPARα),nuclear factor kappa-B(NF-κB),phosphorylated NF-κB(p-NF-κB),NOD-like receptor thermal protein domain associated protein 3(NLRP3),caspase-1,interleukin-1β(IL-1β),and interleukin-18(IL-18)were analyzed via Western blot,ELISA,and immunohistochemistry.Results:Compared with the NC group,the IM group showed significantly shortened colon length(P＜0.05),exhibited mucosal damage,inflammatory cell infiltration,and glandular disorder,along with upregulated protein expression of p-NF-κB,NLRP3,IL-1β,IL-18,and caspase-1(P＜0.05),and downregulated PPARα expression(P＜0.05).After moxibustion intervention,the MO 15 min group demonstrated increased intestinal length(P＜0.05),reduced pathological scores(P＜0.05),significantly downregulated expression of NLRP3,p-NF-κB,IL-1β,and IL-18(P＜0.05),and elevated PPARα expression(P＜0.05),while total NF-κB protein levels remained unchanged.Conclusion:Moxibustion at Tianshu(ST25)acupoint may alleviate 5-FU-induced intestinal mucosal inflammatory responses by activating PPARα to suppress the NF-κB/NLRP3 inflammasome signaling pathway.

Objective:To investigate the effects of calf spleen extract(CSE)on immune function,serum ferritin levels,and adverse reactions in patients with esophageal squamous cell carcinoma undergoing adjuvant chemotherapy after surgery,and to verify its effects on esophageal cancer cell proliferation and apoptosis in vitro.Methods:A total of 96 postoperative chemotherapy patients with esophageal squamous cell carcinoma at Tianjin Cancer Hospital Airport Hospital were selected and randomly divided into control group and treatment group.They were treated with TP regimen as routine,and then treated with CSE in the treatment group.After the treatment was completed,adverse reactions and clinical reactions were observed in the two groups,and immune related indicators and ferritin levels were compared before and after treatment.Compared the disease free survival(DFS)differences between two groups of pa-tients during a follow-up period of 1 and 3 years.Using MTT assay to determine the IC50 value of CSE in esophageal cancer cells.Plate cloning method was used to detect the changes in the proliferation ability of esophageal squamous cell carcinoma cells induced by CSE.Comet assay detected DNA strand damage in esophageal cancer cells caused by CSE.Flow cytometry was used to detect the effect of CSE on apoptosis of esophageal cancer cells.Results:Comparison between the two groups of patients before and after treatment showed that CSE had a significant effect on CD3+T,CD4+T,CD8+T,CD4+T/CD8+T,Iron protein levels had an impact.CSE could alleviate the adverse reactions of postoperative chemotherapy in patients with esophageal cancer,and improve their quality of life and clinical symp-toms.CSE treatment could improve short-term DFS in postoperative patients with esophageal cancer.The IC50 concentration of CSE in esophageal cancer cells was 3.018 mg/ml;CSE could inhibit the proliferation of esophageal cancer cells and increase DNA strand dam-age in esophageal cancer cells.CSE can increase apoptosis of esophageal cancer cells.Conclusion:CSE can inhibit the proliferation of esophageal cancer cells,promote their apoptosis,and improve the quality of life and short-term DFS of esophageal cancer patients after treatment by regulating immune function and reducing adverse reactions.

Thoracic anesthesia with spontaneous respiration represents a form of precision anesthesia meticulously customized to individual patients.Considering the more stringent requirements this anesthesia approach imposes on the regulation of respiratory function,the writing group of the"Consensus of Experts on the Management of Thoracic Anesthesia with Spontaneous Respiration"has formulated elaborate guidelines regarding indications and contraindications,preoperative evaluation,anesthesia implementation,common complications,and treatment strategies.This was accomplished by referencing relevant domestic and international literature and integrating it with actual clinical requirements.The objective is to standardize the rational application of this anesthesia method.

Objective:To investigate the effects of moxibustion at Tianshu(ST25)acupoint on 5-fluorouracil(5-FU)-induced intestinal mucositis(IM)and its underlying mechanisms.Methods:Eighteen C57BL/6 male mice were randomly divided into four groups:normal control(NC),IM model(IM),moxibustion 15 min(MO 15 min),and moxibustion 30 min(MO 30 min).The IM model was established via intraperitoneal injection of 5-FU.Pathological changes in colon tissues were observed using hematoxylin and eosin(HE)staining.Protein expression levels of peroxisome proliferator-activated receptor alpha(PPARα),nuclear factor kappa-B(NF-κB),phosphorylated NF-κB(p-NF-κB),NOD-like receptor thermal protein domain associated protein 3(NLRP3),caspase-1,interleukin-1β(IL-1β),and interleukin-18(IL-18)were analyzed via Western blot,ELISA,and immunohistochemistry.Results:Compared with the NC group,the IM group showed significantly shortened colon length(P＜0.05),exhibited mucosal damage,inflammatory cell infiltration,and glandular disorder,along with upregulated protein expression of p-NF-κB,NLRP3,IL-1β,IL-18,and caspase-1(P＜0.05),and downregulated PPARα expression(P＜0.05).After moxibustion intervention,the MO 15 min group demonstrated increased intestinal length(P＜0.05),reduced pathological scores(P＜0.05),significantly downregulated expression of NLRP3,p-NF-κB,IL-1β,and IL-18(P＜0.05),and elevated PPARα expression(P＜0.05),while total NF-κB protein levels remained unchanged.Conclusion:Moxibustion at Tianshu(ST25)acupoint may alleviate 5-FU-induced intestinal mucosal inflammatory responses by activating PPARα to suppress the NF-κB/NLRP3 inflammasome signaling pathway.

Body weight abnormalities, including overweight, obesity, and underweight, have become a dual public health challenge in Chinese adults: overweight and obesity lead to a variety of chronic complications, while underweight increases the risks of malnutrition, sarcopenia, and organ dysfunction. To systematically address these issues, multidisciplinary experts in endocrinology, sports science, nutrition, and psychiatry from various regions have held multiple weight management seminars. Based on the latest epidemiological data and clinical evidence, they expanded the guideline to include assessment and intervention strategies for underweight, in addition to the core content of obesity management. This guideline outlines the etiological mechanisms, evaluation methods, and multidimensional management strategies for overweight and obesity, covering key areas such as diagnosis and assessment, medical nutrition therapy, exercise prescription, pharmacological intervention, and psychological support. It is intended to provide a scientific and standardized approach to weight management across the adult population, aiming to curb the rising prevalence of obesity, mitigate complications associated with abnormal body weight, and improve nutritional status and overall quality of life.