Selective dorsal neurotomy (SDN) is a surgical treatment for primary premature ejaculation (PE), but there is still no standard surgical procedure for selecting the branches of the dorsal penile nerves to be removed. We performed this study to explore the value of intraoperative neurophysiological monitoring (IONM) of the penile sensory-evoked potential (PSEP) for standard surgical procedures in SDN. One hundred and twenty primary PE patients undergoing SDN were selected as the PE group and 120 non-PE patients were selected as the normal group. The PSEP was monitored and compared between the two groups under both natural and general anesthesia (GA) states. In addition, patients in the PE group were randomly divided into the IONM group and the non-IONM group. During SDN surgery, PSEP parameters of the IONM group were recorded and analyzed. The differences in PE-related outcome measurements between the perioperative period and 3 months' postoperation were compared for the PE patients, and the differences in effectiveness and complications between the IONM group and the non-IONM group were compared. The results showed that the average latency of the PSEP in the PE group was shorter than that in the normal group under both natural and GA states (P < 0.001). Three months after surgery, the significant effective rates in the IONM and non-IONM groups were 63.6% and 34.0%, respectively (P < 0.01), and the difference in complications between the two groups was significant (P < 0.05). IONM might be useful in improving the short-term therapeutic effectiveness and reducing the complications of SDN.
Male ; Humans ; Premature Ejaculation/surgery* ; Intraoperative Neurophysiological Monitoring/methods* ; Prospective Studies ; Neurosurgical Procedures/methods* ; Penis/surgery* ; Retrospective Studies

OBJECTIVEThis study evaluated the influence of alkaline coating of nano-zirconium hydroxide on resin bonding of 10-methacryloxy decyldihydrogen phosphate (MDP)-conditioned zirconia.

METHODSA total of 140 yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) plates were prepared and sandblasted with alumina particles, and then subjected to different surface treatment. Resin bonding specimens were prepared with a MDP-free resin cement and tested for shear bond strength (SBS). X-ray photoelectron spectroscopy (XPS) was used to investigate the formation of chemical bond on the surface of Y-TZP treated successively with nano-zirconium hydroxide coating and MDP containing primer.

RESULTSThe two alkaline coatings increased the 24 h SBS compared to control groups, and groups using nano-zirconium hydroxide coating yielded higher SBS. After thermocycling, no statistical difference was observed between groups, but SBS decreased significantly compared to the 24 h SBS (P<0.05). XPS analysis detected -OH bond on the surface of Y-TZP treated with nano-zirconium hydroxide coating, and -P-O-Zr bond was detected on the surface of Y-TZP treated with nano-zirconium hydroxide coating and MDP-containing primer.

CONCLUSIONSAlkaline coatings improved the bonding of resin to zirconia conditioned with MDP-containing primers.

[Objective]To study the resistance reversion of rapamycin on ovarian cancer cell line SKOV3/DDP,and ex-plore its underlying molecular mechanisms.[Methods]MTT method was used to detect the cell toxicity,drug-resistant multi-ple and reversing multiple of cisplatin-resistant ovarian cancer cell line SKOV3/DDP;Western blot was used to detect the changes of Akt/mTOR Pathway induced by rapamycin.[Results]① MTT detected that when rapamycin concentration was 25,50,100,500 and 1 000 μg/L,its inhibition rates on cisplatin-resistant ovarian cancer cell line SKOV3/DDP were 4.48%,25.30%,35.86%,67.82%,81.43%.The concentration of 25 μg/L was selected to be the reversal concentration,be-cause its maximum rate was less than 5%.②The resistant index(RI)of cisplatin-resistant ovarian cancer cell line SKOV3/DDP was 2.21. ③ The reversal fold of 25 μg/L rapamycin on cisplatin-resistant ovarian cancer cell line SKOV3/DDP was 1.63.④Western blot results:After the addition of rapamycin,expression of p-mTOR and its downstream protein p-p70s6k in SKOV3 and SKOV3/DDP was significantly reduced. Meanwhile,there was a feedback increase in p-Akt.[Conclusions]Rapamycin has a reversal effect on cisplatin-resistant ovarian cancer cell line SKOV3/DDP. Its reversal mechanism may be inhibiting the cell proliferation and promoting cell apoptosis by depressing the expression of p-mTOR and its downstream pro-tein p-p70s6k in Akt/mTOR Pathway.

OBJECTIVETo investigate the molecular basis for a novel human leukocyte antigen (HLA) allele B*5827.

METHODSDNA from the proband was analyzed by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified product was sequenced bidirectionally.

RESULTSAbnormal HLA-B locus was observed and its nucleotide sequence was different from the known HLA-B allele sequences, with highest homology to HLA-B*5820 allele. It differs from HLA-B*5820 by 8 nucleotide substitutions in exon 3, i.e., nt 290 (G > C), nt 346 (T > A), nt 390 (A > C), nt 404 (G > C), nt 413 (C > G), nt 471 (A > G), nt 486 (A > G) and nt 487 (C > A), resulting in an amino acid change from ser > arg at nt 97, phe >tyr at nt 115, ser > arg at nt 130, thr > ala at nt 157 and thr > glu at nt 162. Nucleotide differences of nt 404 (G > C) and nt 413( C > G) did not change amino acid.

CONCLUSIONThe sequences of the novel allele have been submitted to GenBank (access No.GU071234). A novel HLA class I allele B*5827 has been officially assigned by the WHO HLA Nomenclature Committee in Jan. 2010.

Alleles ; Base Sequence ; Cloning, Molecular ; Genotype ; HLA-B Antigens ; chemistry ; genetics ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Analysis, DNA

Objective To analyze the incidence of coronary artery disease (CAD) and outcome of patients with left ventricular noncompaction (LVNC). Methods Fifty-one patients with LVNC evaluated by echocardiography and/or cardiac magnetic resonance (CMR) from January 2006 to August 2010 were retrospectively reviewed. Coronary angiography or MDCT was performed for detecting coronary artery disease. Predictors of the cardiac events were analyzed by Cox regression analysis. Results There were 31 LVNC patients without CAD and 20 LVNC patients with CAD including single vessel coronary disease in 9 cases, double vessel coronary disease in 3 cases, three vessel coronary disease in 5 cases and left main coronary disease in 3 cases. Coronary artery bypass graft and percutaneous coronary intervention (PCI) were performed in 4 patients. Compared to LVNC patients without CAD, mean age ( P = 0. 008 ), incidence of hypertension (65.0% vs. 19. 4% , P = 0. 001 ), diabetes mellitus (40. 0% vs. 12. 9% , P = 0. 026) and hyperlipidemia ( 55.0% vs. 25. 8%, P = 0. 035 ) were significantly higher while NT-proBNP level was significantly lower( P = 0. 049 ) in LVNC patients with CAD. Incidence of major cardiac events was similar in LVNC patients with or without CAD. LogNT-proBNP is the independent prognostic factor for adverse cardiac events in patients with LVNC( HR 3.993,95% CI 1. 140-13. 988 ,P =0. 030). Conclusions Coronary artery disease is common in patients with LVNC and associated with traditional risk factors for CAD. Poor prognosis is associated with increased NT-proBNP but not with CAD in this patient cohort.

Objective To investigate the glycated hemoglobin Alc level (HbAlc) and its role in diagnosing impaired fasting glucose (IFG) and type 2 diabetes mellitus (T2DM) in civil pilots.Methods One hundred and fifty-one civil pilots,who were in routine physical examination in Civil Aviation General Hospital,were chosen as pilot group and 96 common examined personnel were taken as control group.The pilot goup was further analyzed by different HbAlc and FPG level to get the correlation between them.The level of fasting plasma glucose (FPG),lipid profiles,liver and renal function markers and HbAlc were measured and analyzed.Results No difference of HbAlc was observed between pilot and control group.The HbAlc levcl was significantly higher in IFG and T2DM sub-group than that in normal glucose sub-group (P＜0.01).The multiple linear regression analysis indicated that FPG,γ-glutamyl transferase (γ-GT),alanine transaminase (ALT),and serum creatinine (SCr) were the significant and independent influencing factors against HbAlc.The receiver operating characteristic (ROC) curve showed that HbAlc at the level of 5.6％ and 6.2％ was the best cut-off value for diagnosing and screening IFG and T2DM.Conclusions HbAlc may be the key indicator in diagnosing and monitoring IFG and T2DM,as well as in evaluating cardiovascular disease incidence for civil pilot.Besides routinely monitoring FPG in physical examination,HbAlc should be the indicator in assessing glucose metabolism for the pilot with T2DM and taking hypoglycemic drugs.

Objective To investigate the correlation between the serum uric acid (SUA) level and the coronary heart disease (CHD) events in Chinese civil pilots. Methods Fasting blood samples were collected from 46 civil pilots who were likely diagnosed as CHD, and coronary arteriongraphy was conducted for final diagnosis in these subjects. The SUA levels, fasting blood glucose and blood lipid profile were measured and the correlation between CHD events and the above-mentioned biochemical results, history and age were statistically analyzed. Results Eighteen patients (39.1%) were diagnosed as CHD, while 28(60.9%) had no or minor abnormalities determined by coronary arteriongraphy, so as to be classified into CHD or non-CHD group respectively. The differences on age and the association of hypertension, low high density lipoprotein concentrations and hyperuricemia were significant between two groups (P<0.05), but on the association of diabetes, smoking, high cholesterol, high triglyceride and high low density lipoprotein concentrations (P>0.05). The mean SUA level in CHD group was significantly higher than that in non-CHD group [(430.58±89.10) μmol/L vs (341.70±78.11) μmol/L] (t=3.565, P<0.01). Furthermore, the exact Logistic regression indicated that the SUA concentration was the independent risk factor against CHD incidence. Conclusion The elevation of SUA might be an independent risk factor that induced CHD in pilot.

Objective To investigate the glycated hemoglobin Alc level (HbAlc) and its role in diagnosing impaired fasting glucose (IFG) and type 2 diabetes mellitus (T2DM) in civil pilots.Methods One hundred and fifty-one civil pilots,who were in routine physical examination in Civil Aviation General Hospital,were chosen as pilot group and 96 common examined personnel were taken as control group.The pilot goup was further analyzed by different HbAlc and FPG level to get the correlation between them.The level of fasting plasma glucose (FPG),lipid profiles,liver and renal function markers and HbAlc were measured and analyzed.Results No difference of HbAlc was observed between pilot and control group.The HbAlc levcl was significantly higher in IFG and T2DM sub-group than that in normal glucose sub-group (P＜0.01).The multiple linear regression analysis indicated that FPG,γ-glutamyl transferase (γ-GT),alanine transaminase (ALT),and serum creatinine (SCr) were the significant and independent influencing factors against HbAlc.The receiver operating characteristic (ROC) curve showed that HbAlc at the level of 5.6％ and 6.2％ was the best cut-off value for diagnosing and screening IFG and T2DM.Conclusions HbAlc may be the key indicator in diagnosing and monitoring IFG and T2DM,as well as in evaluating cardiovascular disease incidence for civil pilot.Besides routinely monitoring FPG in physical examination,HbAlc should be the indicator in assessing glucose metabolism for the pilot with T2DM and taking hypoglycemic drugs.

Objective To investigate the correlation between the serum uric acid (SUA) level and the coronary heart disease (CHD) events in Chinese civil pilots. Methods Fasting blood samples were collected from 46 civil pilots who were likely diagnosed as CHD, and coronary arteriongraphy was conducted for final diagnosis in these subjects. The SUA levels, fasting blood glucose and blood lipid profile were measured and the correlation between CHD events and the above-mentioned biochemical results, history and age were statistically analyzed. Results Eighteen patients (39.1%) were diagnosed as CHD, while 28(60.9%) had no or minor abnormalities determined by coronary arteriongraphy, so as to be classified into CHD or non-CHD group respectively. The differences on age and the association of hypertension, low high density lipoprotein concentrations and hyperuricemia were significant between two groups (P<0.05), but on the association of diabetes, smoking, high cholesterol, high triglyceride and high low density lipoprotein concentrations (P>0.05). The mean SUA level in CHD group was significantly higher than that in non-CHD group [(430.58±89.10) μmol/L vs (341.70±78.11) μmol/L] (t=3.565, P<0.01). Furthermore, the exact Logistic regression indicated that the SUA concentration was the independent risk factor against CHD incidence. Conclusion The elevation of SUA might be an independent risk factor that induced CHD in pilot.

Objective To characterize the clinical and cardiac MRI features of dilated cardiomypathy ( DCM) and left ventricular noncompaction (LVNC). Methods Compared the clinical and MRI features between 25 patients with LVNC and 21 patients with DCM. The MRI derived diastolic left ventricular wall thickness and the number and degree of noncompaction (NC) were evaluated using the 17-segment model. Results Chest distress, shortness of breath and abnormal ECG were presented in all DCM patients, abnormal ECG was evidenced in 22 LVNC patients and 21 out of 25 LVNC patients presented similar clinical symptoms as DCM patients while the rest 4 LVNC patients were asymptomatic. Left atrial and ventricular dimensions were significantly smaller in LVNC patients compared to DCM patients. The degree of left ventricular (LV) spherical remodeling was significantly greater in patients with DCM (sphericity index, SI = 0. 81 ± 0. 06) than in patients with LVNC ( SI = 0. 74 ± 0. 11, P < 0. 05). The LV ejection fraction ( LVEF) was significantly higher in patients with LVNC (32. 7% ± 14. 2% ) than that in patients with DCM (15. 0% ±5. 1% ). The number of NC segments in LVNC patients (9 ± 1) was significantly higher than the number of hypertrabeculation segment in DCM patients (5 ±2). The left ventricular apex (the 17th segment) was unexceptionally involved in all LVNC patients, while hypertrabeculation was absent in the 17th segment of DCM patients. The NC was more common in the apical and mid segments (16th, 12th and 11th segments) than in basal and mid septal segments (2nd, 3rd, 8th and 9th segments) in both LVNC and DCM patients. The thickness of compacted myocardium of the segments associated with noncompaction appeared thin in two groups. The wall thickness of noncompaction myocardium segments was thicker in LVNC patients than in DCM patients. The end-diastolic NC/C ratio was, on average, higher in patients with LVNC (3. 3 ± 0. 6) than in patients with DCM (1.9 ± 0. 3 ) . Conclusions The clinical manifestation is similar while there are significant differences in the morphology and function of left atria and left ventricle between the LVNC and DCM patients. The different distribution and degree of NC were helpful to differentiate LVNC from DCM.