Objective To investigate the effect of Angelica sinensis polysaccharide (ASP) on intestinal injury in septic rats by regulating the adenosine-monophosphate-activated protein kinase (AMPK)/Nod-like receptor protein 3 (NLRP3) pathway-mediated pyroptosis. Methods A rat model of sepsis was created using cecal ligation and perforation surgery. The successfully modeled rats were separated into a model group;low-,medium-,and high-ASP (ASP-L,ASP-M,ASP-H,respectively) groups;and an AMPK inhibitor group,with 12 rats in each group. Twelve rats in the same period were regarded as the sham group without cecal ligation. Hematoxylin and eosin staining was used to observe the pathological changes in the small intestine of the rats and pathological scoring was performed. TUNEL staining was used to detect intestinal cell pyroptosis;immunohistochemical staining was used to detect NLRP3 expression in small intestine tissue;Enzyme-linked immunosorbent asays were used to determine the serum levels of interleukin (IL)-18 and IL-1β and the activities of D-amino acid oxidase (DAO) and intestinal fatty acid binding protein (iFABP) in the small intestine;and Western blotting was used to determine the levels of gasdermin D (GSDMD),apoptosis-associated speck-like protein containing a CARD (ASC),cleaved caspase-1,and p-AMPK/AMPK proteins in small intestine tissue. Results Compared with the sham group,the model group showed increased small intestine tissue Chiu score;apoptosis rate;and NLRP3,DAO,iFABP,GSDMD,ASC,cleaved caspase-1 and serum IL-18 and IL-1β levels and a decreased p-AMPK/AMPK ratio (P<0.05). Compared to the model group,the ASP-L,ASP-M,and ASP-H groups showed reduced small intestine tissue Chiu score;apoptosis rate;NLRP3,DAO,iFABP,GSDMD,ASC,cleaved caspase-1,and serum IL-18 and IL-1β levels and an increased p-AMPK/AMPK ratio (P<0.05). AMPK inhibitors weakened the inhibitory effect of ASP on small intestine tissue cell apoptosis,and exacerbated intestinal injury (P<0.05). Conclusion ASP inhibited the activation of NLRP3 inflammasomes by activating AMPK expression,thereby inhibiting pyroptosis and alleviating intestinal injury in septic rats.

Objective To investigate the effect of Angelica sinensis polysaccharide (ASP) on intestinal injury in septic rats by regulating the adenosine-monophosphate-activated protein kinase (AMPK)/Nod-like receptor protein 3 (NLRP3) pathway-mediated pyroptosis. Methods A rat model of sepsis was created using cecal ligation and perforation surgery. The successfully modeled rats were separated into a model group;low-,medium-,and high-ASP (ASP-L,ASP-M,ASP-H,respectively) groups;and an AMPK inhibitor group,with 12 rats in each group. Twelve rats in the same period were regarded as the sham group without cecal ligation. Hematoxylin and eosin staining was used to observe the pathological changes in the small intestine of the rats and pathological scoring was performed. TUNEL staining was used to detect intestinal cell pyroptosis;immunohistochemical staining was used to detect NLRP3 expression in small intestine tissue;Enzyme-linked immunosorbent asays were used to determine the serum levels of interleukin (IL)-18 and IL-1β and the activities of D-amino acid oxidase (DAO) and intestinal fatty acid binding protein (iFABP) in the small intestine;and Western blotting was used to determine the levels of gasdermin D (GSDMD),apoptosis-associated speck-like protein containing a CARD (ASC),cleaved caspase-1,and p-AMPK/AMPK proteins in small intestine tissue. Results Compared with the sham group,the model group showed increased small intestine tissue Chiu score;apoptosis rate;and NLRP3,DAO,iFABP,GSDMD,ASC,cleaved caspase-1 and serum IL-18 and IL-1β levels and a decreased p-AMPK/AMPK ratio (P<0.05). Compared to the model group,the ASP-L,ASP-M,and ASP-H groups showed reduced small intestine tissue Chiu score;apoptosis rate;NLRP3,DAO,iFABP,GSDMD,ASC,cleaved caspase-1,and serum IL-18 and IL-1β levels and an increased p-AMPK/AMPK ratio (P<0.05). AMPK inhibitors weakened the inhibitory effect of ASP on small intestine tissue cell apoptosis,and exacerbated intestinal injury (P<0.05). Conclusion ASP inhibited the activation of NLRP3 inflammasomes by activating AMPK expression,thereby inhibiting pyroptosis and alleviating intestinal injury in septic rats.

Objective: To investigate the hospital employee satisfaction and trustworthiness in the background of the trusteeship mode and to find out the problems after trusteeship.Methods: Minnesota satisfaction questionnaire (MSQ) and the self-made questionnaire were used to conduct the survey.Results: The overall employee satisfaction (3.80±0.86) and trustworthiness (3.95±0.77)were higher.The highest level of trustworthiness concerned the cultural connotation (84.8%) followed by the management concept (82.8%) for overall satisfaction.The lowest level of employee satisfaction concerned income and workload (53.7%), followed by the working conditions and environment (55.3%).The administrative staff satisfaction was higher compared to that of medical staff (p=0.001), which showed significant statistical differences.Conclusions: The hospital has made some achievements after the trusteeship system reform, however, it still needs improvement and further strengthening in many aspects.The hospital must always keep abreast of the demands of workforce and improve the staff satisfaction so as to promote its continuous and sustainable development.

BACKGROUND:Recent studies have found that stem cels can directly differentiate into mature myocardial cels or promote their regeneration, providing a new therapeutic strategy for the treatment of myocardial infarction. However, the low cel transplantation rate reduces the myocardial differentiation ability and myocardial repair.
OBJECTIVE: To study the role of heat shock treatment in Sca-1+ cel transplantation for treatment of myocardial infarction in mice.
METHODS:Sca-1+ cels were isolated from the bone marrow of mice using magnetic bead sorting method, and were subjected to heat shock treatment. Animal models of myocardial infarction were made in mice, and then randomized into two groups: heat shock group and non-heat shock group, which were given 1 mL heat shock-treated Sca-1+ cels and 1 mL non-heat shock-treated Sca-1+ celsvia the tail vein, respectively. After transplantation, cel survival, heart function, myocardial cel apoptosis and myocardial fibrosis were detected. Meanwhile, the expressions of heat shock factor (HSP), HSP70 and miR-34a in the left ventricle were measured.
RESULTS AND CONCLUSION:(1) The expression of sry gene in the heat shock group was significantly higher than that in the non-heat shock group. (2) The left ventricular ejection fraction and fractional shortening in the heat shock group were significantly higher than those in the non-heat shock group. The left ventricular end-diastolic diameter and systolic diameter in the heat shock group were significantly lower than those in the non-heat shock group. (3) The cardiac fibrosis and myocardial cel apoptosis in the heat shock group were significantly lower than those in the non-heat shock group. (4) The HSF and HSP70 expression in the left ventricle was significantly higher in the heat shock group than the non-heat shock group, and the miR-34a expression in the left ventricle was significantly lower in the heat shock group than the non-heat shock group. These findings indicate that heat shock-treated Sca-1+ cel transplantation can reduce myocardial apoptosis and infarct size, and improve heart function of mice with myocardial infarction.