Objective To explore the effect of total saponins of diaphragma juglandis fructus(DJF)on testicular function in rats and its possible mechanism.Methods Fifty SD male rats were randomly divided into NC group,MG model group(MG group),DJF low-dose group(LDJF group),DJF medium-dose group(MDJF group)and DJF high-dose group(HDJF group),with 10 rats in each group.The NC group was given daily subcutaneous injection of normal saline 500 mg/kg,and the other groups were given daily subcutaneous injection of D-galactose 500 mg/kg for 8 weeks to establish the subacute aging rat model.After 4 weeks of subcutaneous injection,rats of the LDJF group,MDJF group and HDJF group were given 100 mg/kg,200 mg/kg and 400 mg/kg DJF total saponin extract by gavage,respectively,and rats of the NC group and MG group were given 100 mg/kg normal saline by gavage,respectively,for 28 consecutive days.The body weight of rats in each group was measured,the testicular index and sperm parameters of rats in each group were analyzed,and the pathological changes of testicular tissue were observed.The serum levels of testosterone,oxidation factor superoxide dismutase(SOD)and malondialdehyde(MDA)in each group were detected.The expression of related proteins was detected by Western blot.Results Compared with the NC group,testicular weight,testicular index,serum testosterone and SOD levels,sperm density,sperm survival rate and sperm activity of rats in MG group were decreased,and MDA level of rats in MG group was increased;the protein expressions of Nrf2 and HO-1 in testicular tissue of rats in MG group were down-regulated,and the protein expressions of p53 and p21 in testicular tissue of rats in MG group were up-regulated,with statistically significant differences(P<0.05).Compared with the MG group,testicular weight,testicular index,serum testosterone and SOD levels,sperm density,sperm survival rate and sperm activity of rats in HDJF group were increased,and MDA level of rats in HDJF group was decreased;the protein expressions of Nrf2 and HO-1 in testicular tissue of rats in HDJF group were up-regulated,and the protein expressions of p53 and p21 in testicular tissue of rats in HDJF group were down-regulated,with statistically significant differences(P<0.05).There was no significant difference in the above indexes between LDJF group,MDJF group and MG group(P>0.05).Compared with the NC group,the arrangement of spermatogenic cells in testicular tissue was disordered,the number of spermatogenic cells was reduced,and the number of spermatozoa in seminiferous tubule was reduced in the MG group.Compared with the MG group,the testicular morphology was improved and the number of spermatozoa was increased after DJF total saponins intervention,and the improvement was most obvious in the HDJF group.Conclusion DJF total saponins can effectively ameliorate the decline of testicular functionin in rats,which may be related to activation of Nrf2/HO-1 pathway,enhancement of antioxidant stress level and inhibition of aging-related protein expression.

Objective To explore the effect of total saponins of diaphragma juglandis fructus(DJF)on testicular function in rats and its possible mechanism.Methods Fifty SD male rats were randomly divided into NC group,MG model group(MG group),DJF low-dose group(LDJF group),DJF medium-dose group(MDJF group)and DJF high-dose group(HDJF group),with 10 rats in each group.The NC group was given daily subcutaneous injection of normal saline 500 mg/kg,and the other groups were given daily subcutaneous injection of D-galactose 500 mg/kg for 8 weeks to establish the subacute aging rat model.After 4 weeks of subcutaneous injection,rats of the LDJF group,MDJF group and HDJF group were given 100 mg/kg,200 mg/kg and 400 mg/kg DJF total saponin extract by gavage,respectively,and rats of the NC group and MG group were given 100 mg/kg normal saline by gavage,respectively,for 28 consecutive days.The body weight of rats in each group was measured,the testicular index and sperm parameters of rats in each group were analyzed,and the pathological changes of testicular tissue were observed.The serum levels of testosterone,oxidation factor superoxide dismutase(SOD)and malondialdehyde(MDA)in each group were detected.The expression of related proteins was detected by Western blot.Results Compared with the NC group,testicular weight,testicular index,serum testosterone and SOD levels,sperm density,sperm survival rate and sperm activity of rats in MG group were decreased,and MDA level of rats in MG group was increased;the protein expressions of Nrf2 and HO-1 in testicular tissue of rats in MG group were down-regulated,and the protein expressions of p53 and p21 in testicular tissue of rats in MG group were up-regulated,with statistically significant differences(P<0.05).Compared with the MG group,testicular weight,testicular index,serum testosterone and SOD levels,sperm density,sperm survival rate and sperm activity of rats in HDJF group were increased,and MDA level of rats in HDJF group was decreased;the protein expressions of Nrf2 and HO-1 in testicular tissue of rats in HDJF group were up-regulated,and the protein expressions of p53 and p21 in testicular tissue of rats in HDJF group were down-regulated,with statistically significant differences(P<0.05).There was no significant difference in the above indexes between LDJF group,MDJF group and MG group(P>0.05).Compared with the NC group,the arrangement of spermatogenic cells in testicular tissue was disordered,the number of spermatogenic cells was reduced,and the number of spermatozoa in seminiferous tubule was reduced in the MG group.Compared with the MG group,the testicular morphology was improved and the number of spermatozoa was increased after DJF total saponins intervention,and the improvement was most obvious in the HDJF group.Conclusion DJF total saponins can effectively ameliorate the decline of testicular functionin in rats,which may be related to activation of Nrf2/HO-1 pathway,enhancement of antioxidant stress level and inhibition of aging-related protein expression.

AIM To explore the clinical effects of Jiawei Yanghe Decoction combined with Budesonide and Formoterol Fumarate Powder for Inhalation on patients with mild to moderate bronchial asthma in chronic and persistent period.METHODS One hundred and eighteen patients were randomly assigned into control group(59 cases)for 4-week administration of Budesonide and Formoterol Fumarate Powder for Inhalation,and observation group(59 cases)for 4-week administration of both Jiawei Yanghe Decoction and Budesonide and Formoterol Fumarate Powder for Inhalation.The changes in clinical effects,ACT score,bronchial asthma control rate,pulmonary function indices(FEV1,PEF,FEV1％,PEF％),inflammatory indices(EOS,EOS％,FeNO),TCM syndrome score and incidence of adverse reactions were detected.RESULTS The observation group demonstrated higher total effective rate than the control group(P＜0.05).After the treatment,the two groups displayed increased bronchial asthma control rate,ACT score,PEF(P＜0.05),and decreased TCM syndrome score(P＜0.05),especially for the observation group(P＜0.05);the observation group exhibited increased FEV1,FEV1％,PEF％(P＜0.05),among which FEV1,PEF％were higher than those in the control group(P＜0.05);the observation group showed decreased inflammatory indices(P＜0.05),among which FeNO was lower than that in the control group(P＜0.05).No significant difference in incidence of adverse reactions was found between the two groups(P＞0.05).CONCLUSION For the patients with mild to moderate bronchial asthma in chronic and persistent period,Jiawei Yanghe Decoction combined with Budesonide and Formoterol Fumarate Powder for Inhalation can safely and effectively alleviate clinical symptoms,improve pulmonary functions,airway inflammatory reactions,and enhance bronchial asthma control rate.

Scholarly articles published in the Web of Science database from January 1,2004 to November 10,2024 in the field of medical device utilization management were collected.CiteSpace-based bibliometric analysis of the included literature was performed in terms of the year of publication,region of publication,highly cited literature and highly cited journals and keywords.The Internet of Things(IoT),cloud computing and wearable devices were identified as the current research hotspots in the field of medical device utilization management.References were provided for further research related to medical device utilization management.[Chinese Medical Equipment Journal,2025,46(7):63-67]

Scholarly articles published in the Web of Science database from January 1,2004 to November 10,2024 in the field of medical device utilization management were collected.CiteSpace-based bibliometric analysis of the included literature was performed in terms of the year of publication,region of publication,highly cited literature and highly cited journals and keywords.The Internet of Things(IoT),cloud computing and wearable devices were identified as the current research hotspots in the field of medical device utilization management.References were provided for further research related to medical device utilization management.[Chinese Medical Equipment Journal,2025,46(7):63-67]

AIM To explore the clinical effects of Jiawei Yanghe Decoction combined with Budesonide and Formoterol Fumarate Powder for Inhalation on patients with mild to moderate bronchial asthma in chronic and persistent period.METHODS One hundred and eighteen patients were randomly assigned into control group(59 cases)for 4-week administration of Budesonide and Formoterol Fumarate Powder for Inhalation,and observation group(59 cases)for 4-week administration of both Jiawei Yanghe Decoction and Budesonide and Formoterol Fumarate Powder for Inhalation.The changes in clinical effects,ACT score,bronchial asthma control rate,pulmonary function indices(FEV1,PEF,FEV1％,PEF％),inflammatory indices(EOS,EOS％,FeNO),TCM syndrome score and incidence of adverse reactions were detected.RESULTS The observation group demonstrated higher total effective rate than the control group(P＜0.05).After the treatment,the two groups displayed increased bronchial asthma control rate,ACT score,PEF(P＜0.05),and decreased TCM syndrome score(P＜0.05),especially for the observation group(P＜0.05);the observation group exhibited increased FEV1,FEV1％,PEF％(P＜0.05),among which FEV1,PEF％were higher than those in the control group(P＜0.05);the observation group showed decreased inflammatory indices(P＜0.05),among which FeNO was lower than that in the control group(P＜0.05).No significant difference in incidence of adverse reactions was found between the two groups(P＞0.05).CONCLUSION For the patients with mild to moderate bronchial asthma in chronic and persistent period,Jiawei Yanghe Decoction combined with Budesonide and Formoterol Fumarate Powder for Inhalation can safely and effectively alleviate clinical symptoms,improve pulmonary functions,airway inflammatory reactions,and enhance bronchial asthma control rate.

Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control. Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake. Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors. Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which may pose a risk of infection to humans and animals.The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.

Background and objective Lung cancer is the malignant tumor with the highest incidence rate and the heaviest disease burden in China.In recent years,lung cancer has shown a high incidence trend,seriously affecting the health of the population.In this paper,we analyze the characteristics of lung cancer incidence in 2019 and the trend of incidence rate from 2010-2019 in the tumor registration area of Gansu province,in order to provide a reference basis for the development of lung cancer prevention and control strategies in Gansu province.Methods By analyzing the cases of lung cancer incidence in the tumor registration area of Gansu province in 2019,we calculated the incidence rate,medium incidence rate,world in-cidence rate and other related indexes;we used Joinpoint to calculate the annual percentage change(APC)for trend analysis.Results In 2019,a total of 3757 new cases of lung cancer were reported in Gansu province,accounting for 14.96%of all new malignant tumors.The incidence rate,medium incidence rate and world incidence rate and world rate of lung cancer were 40.52/105,25.78/105,25.86/105;and the cumulative rate of 0-74 years old,and the truncation rate of 35-64 years old were 3.23%,40.03/105,respectively.The incidence of lung cancer rises with age,and is high in the age group of 40 years and above,and the incidence peaks in the male and female populations in the group of 75 years and above,and the group of 80 years and above,respectively.The crude incidence rate of lung cancer in the tumor registration area of Gansu province from 2010-2019 showed an overall increasing trend,and the rate of increase was relatively fast,with an APC 5.39%(P<0.05);Separately,accord-ing to gender,urban and rural areas,the incidence of lung cancer in all populations showed an increasing trend,and the APC of male,female,urban and rural populations were 4.98%,6.39%,6.26%,and 4.64%,respectively(all P<0.05).According to the trend analysis of lung cancer incidence rate by age group,only lung cancer incidence in the age group of 65 years and above increased at an annual average rate of 4.15%(P<0.05).Conclusion The incidence rate of lung cancer in the tumor registration area of Gansu province from 2010 to 2019 shows a rising trend year by year,and there are differences in the incidence of lung cancer in people of different genders,regions and age groups,so comprehensive prevention and control work should be carried out for the key populations of lung cancer incidence.

This study analyzed the characteristics of 118 738 seasonal migrants who were aged 65 and under management by the Health Statistics Center of Hainan Health Commission.Our study revealed that approximately 51.6％of elderly seasonal migrants were male,with around 75.2％aged below 75 years.Elderly seasonal migrants originating from the Northeast region constituted roughly 36.3％of the total,and the majority of them settled in Haikou,Chengmai and Dongfang(each representing over 10％of the total).Of the nine prevalent health conditions surveyed,about 27.6％of the elderly seasonal migrants suffered at least one condition.Haikou,Wanning,Qionghai,and Dongfang received more than 3 000 elderly seasonal migrants with health issues.Considering the availability of healthcare resources,there exists a negative correlation between the distribution of elderly seasonal migrants and the hospitalization service load of doctors in various destination areas.This study recommends Hainan Province further improving the seasonal migrants dynamic survey system,scientifically arranging healthcare resources and services,and systematically enhancing the health service experience of the seasonal migrants.

Objective To observe the value of contrast-enhanced CT radiomics combined with clinical and hematology indicators for predicting lymph node(LN)metastasis(LNM)of esophageal squamous cell carcinoma(ESCC).Methods Totally 218 ESCC patients were retrospectively enrolled.Stage pN1 and pN2 were clustering as LNM(n=90),while stage pN0 were taken as non-LNM(n=128).The patients were divided into training set(n=174)and test set(n=44)at the ratio of 8∶2.In training set,clinical and LN imaging features which could be used to independently judge LNM were screened and a clinical-imaging model was constructed.The hematological indicators that might be associated with ESCC LNM were screened,and a hematological model was constructed.Radiomics features in LN ROI and ESCC volume of interest(VOI)were extracted based on venous-phase contrast-enhanced CT images,and those might be associated with LNM were screened,and a radiomics model was constructed.Finally a combined model was constructed based on all the above features.The efficacy of each model for diagnosing LNM was evaluated with the area under the curve(AUC)of receiver operating characteristic curves,and the clinical net benefit was evaluated using decision curve analysis(DCA).Results Body mass index(BMI)and internal necrosis of target LN were both independent judging factors for ESCC LNM(both P＜0.05),and AUC of clinical-imaging model for diagnosing LNM in training and test sets was 0.747 and 0.687,respectively.Seven hematological indicators were included in hematological model,and AUC in training and test sets was 0.623 and 0.583,respectively.Ten LN radiomics features and 15 ESCC radiomics features were included in radiomics model,and AUC in training and test sets was 0.769 and 0.745,respectively.AUC of the combined model for diagnosing LNM in training and test sets was 0.822 and 0.739,respectively,better than other models in training set(all P＜0.05),but no significantly different in test set(all P＞0.05).DCA showed that combined model had higher net gain than the other models in 0.55-0.80 threshold probability interval.Conclusion Combined model based on venous-phase contrast-enhanced CT radiomics and clinical and hematology indicators could relatively effectively evaluate ESCC LNM,which might bring some promotions in clinical benefit.