BACKGROUND: Scoliosis secondary to neurofibromatosis type 1 (NF1) in children aged <10 years is an important etiology of early-onset scoliosis (EOS). This study was performed to investigate the curve evolution of patients with EOS secondary to NF1 undergoing bracing treatment and to analyze high-risk indicators of rapid curve progression. METHODS: Children with EOS due to NF1 who underwent bracing treatment from 2010 to 2017 were retrospectively reviewed. The angle velocity (AV) at each visit was calculated, and patients with rapid curve progression (AV of >10°/year) were identified. The age at modulation and the AV before and after modulation were obtained. Patients with (n = 18) and without rapid curve progression (n = 10) were statistically compared. RESULTS: Twenty-eight patients with a mean age of 6.5 ± 1.9 years at the initial visit were reviewed. The mean Cobb angle of the main curve was 41.7° ± 2.4° at the initial visit and increased to 67.1° ± 8.6° during a mean follow-up of 44.1 ± 8.5 months. The overall AV was 6.6° ± 2.4°/year for all patients. At the last follow-up, all patients presented curve progression of >5°, and 20 (71%) patients had progressed by >20°. Rapid curve progression was observed in 18 (64%) patients and was associated with younger age at the initial visit and a higher incidence of modulation change during follow-up (t = 2.868, P = 0.008 and <0.001, respectively). The mean AV was 4.4° ± 1.2°/year before modulation and 11.8° ± 2.7°/year after modulation (t = 11.477, P < 0.010). CONCLUSIONS Curve progression of >10°/year is associated with younger age at the initial visit, and modulation change indicated the occurrence of the rapid curve progression phase.
Braces ; Child ; Child, Preschool ; Disease Progression ; Humans ; Neurofibromatosis 1/complications* ; Retrospective Studies ; Scoliosis/diagnostic imaging* ; Treatment Outcome

Objective To evaluate the association of cervical cancer and the G/C single nucleotide polymorphism at codon 72 in p53 gene exon -4 in Chinese population.Methods Literatures were searched in CNKI,VIP,CBM, PubMed,EMBase,the Cochrane Library (Issue 1,2015),WanFang Data Database (1980—2015)with computer. Results Thirteen studies involving 1 780 cervical cancer patients and 1 5 17 healthy volunteers were included.According to the situation of the HPV infection in research object,thirteen studies were divided into two subgroups.The results of meta-analyses in the subgroup of non-HPV infection showed that the Chinese women having the genotype G/G presented no increased relative risk compared to that with the genotype C/C(OR=0.980,95%CI:0.558 -1.723).The allele G showed no increased relative risk compared to the allele C (OR=1.070,95%CI:0.789-1.449)The results of meta-analyses in the subgroup of HPV infection showed that the Chinese women having the genotype G/G presented no increased relative risk compared to that with the genotype C/C(OR=0.891,95%CI:0.213 -3.722).The allele G showed no increased relative risk compared to the allele C (OR =0.981,95%CI:0.493 -1.951 ).Conclusion This meta -analysis demonstrates that G/C single nucleotide polymorphism at codon 72 in p53 gene exon-4 were not associated with cervical cancer in Chinese women.

BACKGROUNDThere were few studies on the relation between changes in libido and incidence of stroke recurrence. The aim of this study was to investigate the relationship between libido decrease at 2 weeks after stroke and recurrent stroke at 1-year.

METHODSIt is a multi-centered, prospective cohort study. The 14 th item of the Hamilton Depression Rating Scale-17 was used to evaluate changes of libido in poststroke patients at 2 weeks. Stroke recurrence was defined as an aggravation of former neurological functional deficit, new local or overall symptoms, or stroke diagnosed at re-admission.

RESULTSAmong 2341 enrolled patients, 1757 patients had completed follow-up data, 533 (30.34%) patients had decreased libido at 2 weeks, and 166 (9.45%) patients had recurrent stroke at 1-year. Multivariate logistic regression analysis showed that, compared with patients with normal libido, the odds ratio (OR) of recurrent stroke in patients with decreased libido was reduced by 41% (OR = 0.59, 95% confidence interval [CI]: 0.40-0.87). The correlation was more prominent among male patients (OR = 0.52, 95% CI: 0.31-0.85) and patients of ≥60 years of age (OR = 0.57, 95% CI: 0.35-0.93).

CONCLUSIONSOne out of three stroke patients in mainland China has decreased libido at 2 weeks after stroke. Decreased libido is a protective factor for stroke recurrence at 1-year, which is more prominent among older male patients.

Aged ; Asian Continental Ancestry Group ; China ; Female ; Humans ; Incidence ; Libido ; physiology ; Male ; Middle Aged ; Prospective Studies ; Risk Factors ; Stroke ; epidemiology

Objective To investigate the effects of hyperbaric oxygen (HBO) on cerebral hemodynamics and ambulatory electroencephalogram(AEEG) of patients with moderate and severe brain trauma and to analyze its therapeutic efficiency. Methods One hundred and forty-two patients with moderate and severe brain trauma were randomly divided into the conventional therapy group (71 cases) and the HBO plus conventional therapy group (71 cases). The conventional therapy group received surgical treatment and conventional drug treatment, while the HBO group received the same treatment as the conventional therapy group and plus 3 courses of HBO treatment, following stabilization of the illness. Thirty normal healthy people who received physical check-ups were used as control. All the patients received Transcranial Doppler (TCD)detection, before treatment and on the 10th and 30th day after treatment. Ambulatory electroencephalogram (AEEG) was detected and the scores of Glasgow coma scale (GCS) were evaluated before treatment and on the 30th day after treatment. The scores of Glasgow outcome scale (GOS) were again measured in a clinical followup, following 6 months of brain trauma. Results Before treatment, Vs, Vm, Vd, PI and RI of the middle cerebral artery (MCA) in all the patients of both the conventional therapy group and HBO plus conventional therapy group were significantly higher than those of the normal control group ( P ＜ 0. 05 ) and the abnormal rates of AEEG accounted for 94. 4％ and 95.8％ respectively. On the 10th day after treatment, Vs, Vm, PI and RI in the conventional therapy group decreased significantly (P ＜ 0. 05), but were still higher than those of the normal control group ( P ＜ 0. 05 ). Vs, Vm, PI and RIin the HBO plus conventional therapy group decreased more significantly, when compared with those of the conventional therapy group ( P ＜ 0. 05 ),however, Vs, Vm and PI were still higher than those of the normal control group ( P ＜ 0. 05 ). On the 30th day after treatment, Vs and Vm in the conventional therapy group were lower than those of the normal control group (P＜0. 05), but, PI and RI were still higher than those of the normal control group (P ＜0.05). No significant differences in Vs, Vm, Vd, PI and RIcould be noted between the HBO plus conventional therapy group and the normal control group (P ＞0. 05 ). On the 30th day after treatment, abnormal rates of AEEG of both groups decreased significantly than those before treatment ( P ＜ 0. 05 ), while scores of GCS increased significantly than those before treatment ( P ＜ 0. 05 ) and more significant differences could be noticed in the HBO plus conventional therapy group, when compared with those of the conventional therapy group (P ＜ 0. 05). Six months after brain trauma, GOS scores indicated that the number of patients with good prognosis in the HBO plus conventional therapy group increased, and mortality rate decreased obviously, when compared with those of the conventional therapy group (P ＜ 0. 05 ). Conclusions HBO therapy could alleviate cerebral vascular spasms, regulate cerebral blood flow and promote the electrophysiological activity and function of neurons, thus enhancing therapeutic efficiency of the patients with moderate and severe traumatic brain injury.

Objective To investigate the effects of hyperbaric oxygen (HBO) on cerebral hemodynamics and ambulatory electroencephalogram(AEEG) of patients with moderate and severe brain trauma and to analyze its therapeutic efficiency. Methods One hundred and forty-two patients with moderate and severe brain trauma were randomly divided into the conventional therapy group (71 cases) and the HBO plus conventional therapy group (71 cases). The conventional therapy group received surgical treatment and conventional drug treatment, while the HBO group received the same treatment as the conventional therapy group and plus 3 courses of HBO treatment, following stabilization of the illness. Thirty normal healthy people who received physical check-ups were used as control. All the patients received Transcranial Doppler (TCD)detection, before treatment and on the 10th and 30th day after treatment. Ambulatory electroencephalogram (AEEG) was detected and the scores of Glasgow coma scale (GCS) were evaluated before treatment and on the 30th day after treatment. The scores of Glasgow outcome scale (GOS) were again measured in a clinical followup, following 6 months of brain trauma. Results Before treatment, Vs, Vm, Vd, PI and RI of the middle cerebral artery (MCA) in all the patients of both the conventional therapy group and HBO plus conventional therapy group were significantly higher than those of the normal control group ( P ＜ 0. 05 ) and the abnormal rates of AEEG accounted for 94. 4％ and 95.8％ respectively. On the 10th day after treatment, Vs, Vm, PI and RI in the conventional therapy group decreased significantly (P ＜ 0. 05), but were still higher than those of the normal control group ( P ＜ 0. 05 ). Vs, Vm, PI and RIin the HBO plus conventional therapy group decreased more significantly, when compared with those of the conventional therapy group ( P ＜ 0. 05 ),however, Vs, Vm and PI were still higher than those of the normal control group ( P ＜ 0. 05 ). On the 30th day after treatment, Vs and Vm in the conventional therapy group were lower than those of the normal control group (P＜0. 05), but, PI and RI were still higher than those of the normal control group (P ＜0.05). No significant differences in Vs, Vm, Vd, PI and RIcould be noted between the HBO plus conventional therapy group and the normal control group (P ＞0. 05 ). On the 30th day after treatment, abnormal rates of AEEG of both groups decreased significantly than those before treatment ( P ＜ 0. 05 ), while scores of GCS increased significantly than those before treatment ( P ＜ 0. 05 ) and more significant differences could be noticed in the HBO plus conventional therapy group, when compared with those of the conventional therapy group (P ＜ 0. 05). Six months after brain trauma, GOS scores indicated that the number of patients with good prognosis in the HBO plus conventional therapy group increased, and mortality rate decreased obviously, when compared with those of the conventional therapy group (P ＜ 0. 05 ). Conclusions HBO therapy could alleviate cerebral vascular spasms, regulate cerebral blood flow and promote the electrophysiological activity and function of neurons, thus enhancing therapeutic efficiency of the patients with moderate and severe traumatic brain injury.

OBJECTIVEto evaluate the application of GeneSearch(TM) breast lymph node assay in intraoperative detection of metastases in sentinel lymph node (SLN) from breast cancer patients.

METHODSa total of 225 SLN from 88 patients was prospectively studied. Each SLN was cut into 2 mm slabs which were examined by intraoperative imprint cytology (IIC) first, followed by GeneSearch assay and post-operative serial sectioning. GeneSearch used real-time fluorescence quantitative RT-PCR technology to detect the expression of CK19 and mammaglobin in SLN. The results of GeneSearch assay were correlated with those of IIC and post-operative serial sectioning.

RESULTSamongst the 88 cases studied, 225 SLNs were found, and obvious metastatic carcinoma cells were identified in 27 SLNs and micrometastasis in 9 SLNs. One hundred and eight-nine SLNs were considered as "negative" (with "isolated tumor cells" present in 5 SLNs). The turn-around time of intraoperative GeneSearch assay ranged from 35 to 45 minutes (mean = 40 minutes). The concordance rate between GeneSearch assay and post-operative serial sectioning was 95.6% (215/225), with a sensitivity of 86.1% (31/36), compared with 94.7% (213/225) and 72.2% (26/36) respectively for IIC. The size of metastatic foci correlated with the Ct value of CK19 and mammaglobin (P < 0.01).

CONCLUSIONSGeneSearch assay for intraoperative detection of metastase in SLN has a satisfactory performance and demonstrates a relatively higher sensitivity than IIC. The potential clinical application still requires further evaluation of larger number of cases.

Adult ; Aged ; Breast Neoplasms ; metabolism ; pathology ; surgery ; Breast Neoplasms, Male ; metabolism ; pathology ; surgery ; Carcinoma, Ductal, Breast ; metabolism ; pathology ; surgery ; Carcinoma, Intraductal, Noninfiltrating ; metabolism ; pathology ; surgery ; Female ; Humans ; Intraoperative Period ; Keratin-19 ; metabolism ; Lymph Nodes ; metabolism ; pathology ; surgery ; Lymphatic Metastasis ; Male ; Mammaglobin A ; Mastectomy ; methods ; Middle Aged ; Neoplasm Proteins ; metabolism ; Sensitivity and Specificity ; Sentinel Lymph Node Biopsy ; methods ; Uteroglobin ; metabolism

Objective To investigate effects of hyperbaric oxygen (HBO) on brainstem auditory evoked potential (BAEP) and somatic evoked potential (SEP)of patients with moderate and severe trauma tic brain injury and to analyze its therapeutic efficiency. Methods One hundred and thirty-four patients with moderate and severe trauma brain injury were randomly divided into the control group (67 cases) and the HBO adjunctive therapy group (HBO group, 67 cases). The control group received essential neurosurgical treatment and conventional drug treatment, and the HBO group was given HBO therapy for three sessions in addition to the same treatment received by the control group. BAEP and SEP were detected and the scores of Glasgow coma scale (GCS) were measured before and after treatment. Glasgow outcome scale (GOS) were evaluated in follow - ups 6 months after injury. Results Abnormality of BAEP, SEP and GCS for the patients of both groups showed no statistical difference before treatment (P > 0.05). After treatment, significant improvement could be seen in BAEP of the control group(P <0. 05), and more significant improvement was noted in SEP (P <0.01), GCS also increased significantly in the control group (P <0.05). For the HBO group, BAEP and SEP all improved

Objective To investigate effects of hyperbaric oxygen (HBO) on brainstem auditory evoked potential (BAEP) and somatic evoked potential (SEP)of patients with moderate and severe trauma tic brain injury and to analyze its therapeutic efficiency. Methods One hundred and thirty-four patients with moderate and severe trauma brain injury were randomly divided into the control group (67 cases) and the HBO adjunctive therapy group (HBO group, 67 cases). The control group received essential neurosurgical treatment and conventional drug treatment, and the HBO group was given HBO therapy for three sessions in addition to the same treatment received by the control group. BAEP and SEP were detected and the scores of Glasgow coma scale (GCS) were measured before and after treatment. Glasgow outcome scale (GOS) were evaluated in follow - ups 6 months after injury. Results Abnormality of BAEP, SEP and GCS for the patients of both groups showed no statistical difference before treatment (P > 0.05). After treatment, significant improvement could be seen in BAEP of the control group(P <0. 05), and more significant improvement was noted in SEP (P <0.01), GCS also increased significantly in the control group (P <0.05). For the HBO group, BAEP and SEP all improved

OBJECTIVETo explore the prevalence of vision, mental, audibility, language, psychiatry, extremity, and influence factors in the 0 - 7 year olds.

METHODSA total number of 77,727 0 - 7 year old children living in Shenzhen city were tested with tree phase screening under the Chinese standard of evaluation in disabilities.

RESULTSThe prevalence of all disabilities was 5.59 per thousand (adjusted rate was 8.49 per thousand with a false negative of 3.1 per thousand ). The prevalence of mental disease was the highest (1.88 per thousand, with adjusted rate 3.43 per thousand ), the prevalence of language disability was 1.88 per thousand (including retarded language development, with adjusted rate 3.43 per thousand ). The prevalence rates of psychiatry, extremity and audibility disability were 1.59 per thousand, 1.56 per thousand, 1.11 per thousand respectively with of vision the lowest (0.37 per thousand ). The prevalence of all disabilities, audibility, language and mental was on the increase with age. The difference was statistically significant. Among all different age groups regarding psychiatric disease, the highest fell in the 2 - 4 year olds. The prevalence of extremity was not statistically different among age groups. The suspected agents of disease which occurred before or during pregnancy took up 45.7%.

CONCLUSIONThe prevalence of six kinds disabilities in Shenzhen was about 10 per thousand lower than that of the samples of the nation in 1989, but two times higher than that of similar studies in Japan. The prevalence rates of language and psychiatric disease were higher than that of the nation in 1989. The causation should be further studied.

Age Factors ; Child ; Child, Preschool ; China ; epidemiology ; Cross-Sectional Studies ; Disabled Children ; Female ; Humans ; Infant ; Infant, Newborn ; Language Disorders ; epidemiology ; Male ; Mental Disorders ; epidemiology ; Prevalence ; Vision Disorders ; epidemiology