Objective To analyze the level of occupational health literacy (OHL) and mental health status in key populations in Jiangsu Province, and explore the impact of OHL on occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms. Methods A total of 11 181 workers from Jiangsu Province from April to December 2023 were selected as the research subjects using a combination of stratified cluster random sampling and probability proportional sampling method. The OHL levels of the workers, as well as their occupational stress, depression symptoms, anxiety symptoms and insomnia symptoms, were investigated using the Occupational Health Literacy Questionnaire of National Key Populations (including Occupational Health Literacy Scale, Core Occupational Stress Scale, Patient Health Questionaire-9, Generalized Anxiety Disorder 7-item Scale, and Self-Sleep Management Questionnaire). Results The OHL level of the research subjects was 41.7%, and the literacy levels of the four dimensions of occupational health legal knowledge, basic knowledge of occupational health protection, basic skills of occupational health protection, and healthy working style and behavior were 40.6%, 76.4%, 28.0% and 54.4%, respectively. The detection rates of occupational stress, depression symptoms, anxiety symptoms and insomnia symptoms were 21.8%, 17.7%, 26.4% and 29.6%, respectively. The result of multivariable logistic analysis showed that participants with adequate OHL had a lower risk of occupational stress, depressive symptoms, anxiety symptoms, and insomnia symptoms than those without adequate OHL (all P<0.01) after controlling individual and occupational confounding factors. The odds ratios and their 95% confidence intervals were 0.448(0.402-0.499), 0.664(0.593-0.742), 0.705(0.639-0.777) and 0.885(0.809-0.969), respectively. Conclusion OHL is an independent influencing factor for occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms in key populations from Jiangsu Province. Effective measures should be taken to improve the OHL level of workers, to reduce the risks of occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms.

Objective:To explore the expression and significance of integrin subunit β6 (ITGB6) in gastric cancer.Methods:The expression levels of ITGB6 were measured in 408 gastric cancer tissues and 211 adjacent normal tissues in the gene expression profiling interactive analysis (GEPIA) database. The relationship between the expression of ITGB6 and the survival rate of the patients was determined using the Kaplan-Meier method. The glass sections of gastric cancer tissues and adjacent normal tissue wax blocks from the gastric adenocarcinoma patients (78 cases) who underwent radical tumor resection in the Department of Gastrointestinal Surgery of the Second Hospital of Tianjin Medical University from October 2013 to October 2019 were collected. The expression of ITGB6 in gastric cancer tissues and adjacent normal tissues was examined by immunohistochemical staining, and the relationship between the expression of ITGB6 and clinicopathological features of gastric cancer patients was analyzed. MGC-803 and AGS cells were selected, and the expression of ITGB6 gene was inhibited by silencing ITGB6 gene by transfection of lentiviral vectors and control vectors with short hairpin RNA (shRNA). The relative expression levels of mRNA and protein of ITGB6 were detected by real-time reverse transcription-PCR (RT-qPCR) and Western blotting, respectively. The effect of ITGB6 on the proliferation of MGC-803 and AGS cells in vitro was detected by clonal formation assay and MTT assay, and the effects of ITGB6 on tumor volume in mice were further verified by animal experiments. Results:The relative expression level of ITGB6 in gastric cancer tissues was higher than that in normal tissues, and the difference was statistically significant ( P<0.05). High expression of ITGB6 was significantly associated with shorter disease-free survival of patients ( P=0.006 5). Immunohistochemical staining showed that the expression level of ITGB6 was higher in gastric cancer tissues than that in adjacent normal tissues. Compared with the low expression group, ITGB6 expression in the high expression group was positively correlated with tumor differentiation ( χ2=4.468, P=0.035) and tumor stage ( χ2=6.056, P=0.014). In MGC-803 and AGS cells, the relative expression levels of ITGB6 mRNA in transfection group (0.4±0.1, 0.5±0.1) were lower than those in control group (1.0±0.1, 1.0±0.1), and the differences were statistically significant (all P<0.05). The relative expression levels of ITGB6 protein in transfection group (0.3±0.1, 0.3±0.1) were lower than those in control group (1.0±0.1, 1.0±0.1), and the differences were statistically significant (all P<0.05). In MGC-803 and AGS cells, the numbers of cloned cells in transfection group [(26±2, 29±3) unit] were lower than those in control group [(93±9, 112±10) unit], and the differences were statistically significant (all P<0.05). The absorbance ( A) values in the transfection group (0.3±0.1, 0.4±0.1) were lower than those in the control group (1.0±0.2, 1.0±0.1), and the differences were statistically significant (all P<0.05). The tumor volume in transfection group [(184±14) cm 3] was smaller than that in the control group [(252±26) cm 3], and the difference was statistically significant ( P<0.05). The relative level of ITGB6 protein expression in the transfection group (0.3±0.1) was lower than that in the control group (1.0±0.2), and the difference was statistically significant ( P<0.05). Conclusions:The expression of ITGB6 is increased in gastric cancer tissues, and the proliferation of gastric cancer cells can be inhibited by knocking down ITGB6.

Whether it is the long-term health maintenance of the space station in orbit,or the research on aerospace medical issues,multiple physiological indicators reflecting human physiological functions need to be monitored.With the development of wearable physiological detection technology,the requirements for comfort,low load,and human-machine friendly during the continuous collection and detection of physiological information are becoming increasingly high.This paper designs a set of wearable multi-physiological parameter detection system based on flexible electrodes using fabric electrodes as signal sensors.The system includes hardware such as intelligent clothing and physiological signal detection modules,as well as physiological signal display and storage software,which achieves synchronous detection of multiple physiological signals such as ECG,respiration,and EMG.The design ideas,implementation method,key technologies,and results for collecting,detecting,and processing physiological signals under non-steady state conditions are introduced in this paper.The experimental results show that the system can reliably detect physiological signals such as ECG,respiration,and EMG during motion in flight.

Background Cumulative fatigue without intervention will seriously threaten the physical and mental health of workers. Shift work and life satisfaction are strongly associated with fatigue accumulation. Objective To explore the effects of life satisfaction, shift work, and their interaction on cumulative fatigue in petrochemical employees, and to provide a scientific basis for preventing cumulative fatigue. Methods All staff of a petrochemical enterprise were selected by cluster sampling for a cross-sectional study from July to October 2021 in Jiangsu Province. A questionnaire designed by the project team was used to collect information on shift work; and life satisfaction and cumulative fatigue were investigated by the World Health Organization Five-item Well-Being Index and the Self-diagnosis Checklist for Assessment of Worker’s Fatigue Accumulation respectively. A logistic regression model was used to analyze the influences of life satisfaction and shift work on cumulative fatigue. Multiplicative and additive models were applied to analyze the interaction effect of life satisfaction and shift work. Results A total of 4066 questionnaires were returned, of which 3763 were valid, with an effective recovery rate of 92.5%. The percentage of cumulative fatigue in the petrochemical employees was 63.2% (2377/3763), and the percentages of low life satisfaction and shift work in the petrochemical employees were 53.6% (2016/3763) and 54.2% (2041/3763), respectively. The results of univariate analysis showed no significant difference in cumulative fatigue among different marital status groups (P=0.176), and there were statistically significant differences in cumulative fatigue among the petrochemical employees in different groups of age, gender, educational level, average monthly income, job title, length of service, working hours, night shift, smoking, drinking, physical exercise, life satisfaction, and shift work (P<0.001). After adjustment for covariates such as age, gender, educational level, average monthly income, job title, length of service, working hours, night shift, smoking, drinking, and physical activity, the unconditional logistic regression model showed that the risk of reporting cumulative fatigue in high life satisfaction participants was 0.129 (95%CI: 0.109, 0.154) times of that in participants of low life satisfaction; the risk of reporting cumulative fatigue in shift work participants was 3.792 (95%CI: 2.713, 5.300) times of that in no shift work participants; and the risk of reporting cumulative fatigue in participants with both high life satisfaction and shift work was 0.105 (95%CI: 0.081, 0.135) times of that in participants with low life satisfaction and shift work. The relative excess risk due to interaction, the attributable proportion due to interaction, and the synergy index of coexisting life satisfaction and shift work were −5.504 (95%CI: −7.247, −3.760), −4.728 (95%CI: −7.575, −1.880), and 0.029 (95%CI: 0.002, 0.351) respectively, which suggested that life satisfaction and shift work have an additive interaction effect on cumulative fatigue. A significant multiplicative interaction was also found between life satisfaction and shift work (OR=0.688, 95%CI: 0.476, 0.936). Conclusion Life satisfaction and shift work are the influencing factors of cumulative fatigue among petrochemical employees, and they interact with each other on the risk of cumulative fatigue. High life satisfaction can reduce the risk of accumulated fatigue associated with shift work.