Objective To analyze the level of occupational health literacy (OHL) and mental health status in key populations in Jiangsu Province, and explore the impact of OHL on occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms. Methods A total of 11 181 workers from Jiangsu Province from April to December 2023 were selected as the research subjects using a combination of stratified cluster random sampling and probability proportional sampling method. The OHL levels of the workers, as well as their occupational stress, depression symptoms, anxiety symptoms and insomnia symptoms, were investigated using the Occupational Health Literacy Questionnaire of National Key Populations (including Occupational Health Literacy Scale, Core Occupational Stress Scale, Patient Health Questionaire-9, Generalized Anxiety Disorder 7-item Scale, and Self-Sleep Management Questionnaire). Results The OHL level of the research subjects was 41.7%, and the literacy levels of the four dimensions of occupational health legal knowledge, basic knowledge of occupational health protection, basic skills of occupational health protection, and healthy working style and behavior were 40.6%, 76.4%, 28.0% and 54.4%, respectively. The detection rates of occupational stress, depression symptoms, anxiety symptoms and insomnia symptoms were 21.8%, 17.7%, 26.4% and 29.6%, respectively. The result of multivariable logistic analysis showed that participants with adequate OHL had a lower risk of occupational stress, depressive symptoms, anxiety symptoms, and insomnia symptoms than those without adequate OHL (all P<0.01) after controlling individual and occupational confounding factors. The odds ratios and their 95% confidence intervals were 0.448(0.402-0.499), 0.664(0.593-0.742), 0.705(0.639-0.777) and 0.885(0.809-0.969), respectively. Conclusion OHL is an independent influencing factor for occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms in key populations from Jiangsu Province. Effective measures should be taken to improve the OHL level of workers, to reduce the risks of occupational stress, depressive symptoms, anxiety symptoms and insomnia symptoms.

Abstract: Objective To express Mycobacterium tuberculosis Rv2626c protein in Escherichia coli (E. coli) and study the antigenicity of the purified recombinant Rv2626c protein. Methods The amino acid sequence of Rv2626c protein from Mycobacterium tuberculosis H37Rv strain (accession number: CCP45424.1) in GenBank was retrieved and converted into the corresponding DNA sequence according to the codon preference of E. coli. This DNA sequence was synthesized and cloned into pET24a(+) plasmid to construct pET24a(+)-Rv2626c recombinant plasmid. This plasmid was transformed into E. coli BL21(DE3) cells, and the expression of Rv2626c protein was induced under various conditions of isopropyl β-D-thiogalactopyranoside (IPTG) concentrations, temperature, and period. The recombinant Rv2626c protein was identified by SDS-PAGE and Western Blot. The recombinant Rv2626c protein was purified by nickel chelate affinity chromatography and used to immunize violet blue rabbits to prepare anti-Rv2626c anti-serum. The specificity and titer of the serum were respectively detected by Western Blot and enzyme-linked immunosorbent assay (ELISA). Results The recombinant plasmid pET24a(+)-Rv2626c was successfully constructed. SDS-PAGE analysis showed that recombinant Rv2626c was expressed in the recombinant plasmid transformed E. coli with IPTG induction, with a molecular weight of about 14 500, and the size was consistent with the expectation. The optimal expression condition for recombinant Rv2626c protein was at 31 ℃ with 1.0 mmol/L IPTG for 6 hours. The target protein was mainly present in a soluble form, which was consistent with the results of Western blot. The hyperimmunized serum with recombinant Rv2626c protein vaccination showed good specificity, with a titer of 1∶ 256 000 detected by ELISA. Conclusions Mycobacterium tuberculosis Rv2626c protein is successfully expressed in E. coli, and the purified protein has good purity and antigenic activity, laying the foundation for further reveals of its biological functions.

Objective:To investigate the differences in acoustic characteristics between older patients with dysarthria resulting from anterior and posterior circulation cerebral infarctions.Methods:A case-control study was conducted.Sixty hospitalized older patients with dysarthria were selected and divided into two groups: the anterior circulation cerebral infarction group and the posterior circulation cerebral infarction group, each comprising 30 cases.Additionally, thirty healthy individuals aged 65 and above were included as a control group.The subjective evaluation of the patients' overall phonetic function was conducted using the GRBAS scale.Objective parameters, including fundamental frequency(F0), Jitter, Shimmer, maximum phonation time(MPT), maximum sound pressure level(SPLmax), minimum sound pressure level(SPLmin), and the dysphonia severity index(DSI), were collected using the DIVAS2.5 voice analysis system.We analyzed the acoustic characteristics across the three groups: patients with dysarthria and healthy subjects.Results:The grade(G), roughness(R), breathiness(B), asthenia(A), and strain(S)scores of patients in both the anterior and posterior circulation cerebral infarction groups were significantly higher than those of the healthy control group( F=16.574, 39.793, 46.309, 52.154, 25.603; all P<0.001).Furthermore, the roughness(R)and strain(S)of the voice in the anterior circulation cerebral infarction group were significantly elevated compared to the posterior circulation cerebral infarction group, whereas the breathiness(B), asthenia(A), and grade(G)scores in the posterior circulation cerebral infarction group were significantly higher than those in the anterior circulation cerebral infarction group(all P<0.001).The fundamental frequency value(F0)of the voice in patients with anterior circulation cerebral infarction was significantly greater than that of both the posterior circulation cerebral infarction group and the healthy control group( F=39.050, P<0.001).In contrast, the fundamental frequency value(F0)of patients with posterior circulation cerebral infarction was lower than that of the healthy control group( P=0.003).Additionally, the Jitter value in the anterior circulation cerebral infarction group was higher than in both the posterior circulation cerebral infarction group and the healthy control group( F=64.976, P<0.001).The Shimmer value in the anterior circulation cerebral infarction group was lower than that in the posterior circulation cerebral infarction group but higher than that in the healthy control group(both P<0.001).Finally, the values of MPT, SPLmin and SPL max, DSI in the anterior circulation cerebral infarction group were higher than those in the posterior circulation cerebral infarction group and lower than those in the healthy control group( F=90.406, 24.003, 16.164; all P<0.001); the value of DSI in the anterior circulation cerebral infarction group was lower than in both the posterior circulation cerebral infarction group and the healthy control group( F=87.921, P<0.001). Conclusions:There are notable differences in the acoustic characteristic parameters of dysarthria resulting from injuries at various anatomical sites in older patients with cerebral infarction.In practical clinical settings, a comprehensive evaluation of dysarthria in these patients should integrate the anatomical location of the injury, subjective symptom assessment, and objective analysis of acoustic characteristics to inform precise and personalized rehabilitation strategies.

Objective:To investigate the differences in acoustic characteristics between older patients with dysarthria resulting from anterior and posterior circulation cerebral infarctions.Methods:A case-control study was conducted.Sixty hospitalized older patients with dysarthria were selected and divided into two groups: the anterior circulation cerebral infarction group and the posterior circulation cerebral infarction group, each comprising 30 cases.Additionally, thirty healthy individuals aged 65 and above were included as a control group.The subjective evaluation of the patients' overall phonetic function was conducted using the GRBAS scale.Objective parameters, including fundamental frequency(F0), Jitter, Shimmer, maximum phonation time(MPT), maximum sound pressure level(SPLmax), minimum sound pressure level(SPLmin), and the dysphonia severity index(DSI), were collected using the DIVAS2.5 voice analysis system.We analyzed the acoustic characteristics across the three groups: patients with dysarthria and healthy subjects.Results:The grade(G), roughness(R), breathiness(B), asthenia(A), and strain(S)scores of patients in both the anterior and posterior circulation cerebral infarction groups were significantly higher than those of the healthy control group( F=16.574, 39.793, 46.309, 52.154, 25.603; all P<0.001).Furthermore, the roughness(R)and strain(S)of the voice in the anterior circulation cerebral infarction group were significantly elevated compared to the posterior circulation cerebral infarction group, whereas the breathiness(B), asthenia(A), and grade(G)scores in the posterior circulation cerebral infarction group were significantly higher than those in the anterior circulation cerebral infarction group(all P<0.001).The fundamental frequency value(F0)of the voice in patients with anterior circulation cerebral infarction was significantly greater than that of both the posterior circulation cerebral infarction group and the healthy control group( F=39.050, P<0.001).In contrast, the fundamental frequency value(F0)of patients with posterior circulation cerebral infarction was lower than that of the healthy control group( P=0.003).Additionally, the Jitter value in the anterior circulation cerebral infarction group was higher than in both the posterior circulation cerebral infarction group and the healthy control group( F=64.976, P<0.001).The Shimmer value in the anterior circulation cerebral infarction group was lower than that in the posterior circulation cerebral infarction group but higher than that in the healthy control group(both P<0.001).Finally, the values of MPT, SPLmin and SPL max, DSI in the anterior circulation cerebral infarction group were higher than those in the posterior circulation cerebral infarction group and lower than those in the healthy control group( F=90.406, 24.003, 16.164; all P<0.001); the value of DSI in the anterior circulation cerebral infarction group was lower than in both the posterior circulation cerebral infarction group and the healthy control group( F=87.921, P<0.001). Conclusions:There are notable differences in the acoustic characteristic parameters of dysarthria resulting from injuries at various anatomical sites in older patients with cerebral infarction.In practical clinical settings, a comprehensive evaluation of dysarthria in these patients should integrate the anatomical location of the injury, subjective symptom assessment, and objective analysis of acoustic characteristics to inform precise and personalized rehabilitation strategies.

Objective:To observe the efficacy of transurethral electrocoagulation combined with hyperbaric oxygen in the treatment of hemorrhagic radiation cystitis.Methods:A total of 76 patients with hemorrhagic radiation cystitis admitted to Heji Hospital Affiliated to Changzhi Medical College of Shanxi Province from June 2014 to April 2020 were selected as research subjects and randomly divided into control group ( n=38) and combination group ( n=38). The patients in the control group were treated with transurethral electrocoagulation, and the patients in the combination group were treated with hyperbaric oxygen on the basis of the treatments in the control group. The clinical efficacies of the two groups were compared. The urodynamic indexes were evaluated by urethral syndrome score (USS), overactive bladder symptom score (OABSS), interstitial cystitis symptom score (ICSI), and visual analogue scale (VAS) for pain. Results:After treatment, the total clinical effective rate of the combination group (94.74%, 36/38) was significantly higher than that of the control group (81.58%, 31/38), with statistically significant difference ( P<0.05). Compared with those before treatment, in both groups, the bladder capacity and micturition volume increased significantly, while maximum micturition pressure decreased significantly; all showing statistically significant differences ( P<0.05). Moreover, the improvements of bladder capacity, micturition volume, and maximum micturition pressure in the combination group were greater than those in the control group, among which the improvement of micturition volume in the combination group had statistically significant difference from that of the control group ( P<0.05). The USS score, the frequency of urination, and the occurrence of urgent urination and urinary incontinence in both groups decreased compared with those before treatment, all showing statistically significant differences; moreover, the combination group had better improvements in USS score, the frequency of urination, and the occurrence of urgent urination and urinary incontinence than the control group, among which the USS score, the frequency of urination, and the occurrence of urinary incontinence in the combination group had statistically significant differences from those in the control group ( P<0.05). After treatment, the ICSI and VAS scores in both groups decreased, and the two scores in the combination group were significantly lower than those in the control group, with statistically significant differences ( P<0.05). Conclusion:Hyperbaric oxygen combined with transurethral electrocoagulation has a significant effect on hemorrhagic radiation cystitis, which can effectively improve the urodynamic properties of patients, reduce USS, OABSS, ICSI, and VAS scores, and promote the prognosis.

Objective:To observe the efficacy of transurethral electrocoagulation combined with hyperbaric oxygen in the treatment of hemorrhagic radiation cystitis.Methods:A total of 76 patients with hemorrhagic radiation cystitis admitted to Heji Hospital Affiliated to Changzhi Medical College of Shanxi Province from June 2014 to April 2020 were selected as research subjects and randomly divided into control group ( n=38) and combination group ( n=38). The patients in the control group were treated with transurethral electrocoagulation, and the patients in the combination group were treated with hyperbaric oxygen on the basis of the treatments in the control group. The clinical efficacies of the two groups were compared. The urodynamic indexes were evaluated by urethral syndrome score (USS), overactive bladder symptom score (OABSS), interstitial cystitis symptom score (ICSI), and visual analogue scale (VAS) for pain. Results:After treatment, the total clinical effective rate of the combination group (94.74%, 36/38) was significantly higher than that of the control group (81.58%, 31/38), with statistically significant difference ( P<0.05). Compared with those before treatment, in both groups, the bladder capacity and micturition volume increased significantly, while maximum micturition pressure decreased significantly; all showing statistically significant differences ( P<0.05). Moreover, the improvements of bladder capacity, micturition volume, and maximum micturition pressure in the combination group were greater than those in the control group, among which the improvement of micturition volume in the combination group had statistically significant difference from that of the control group ( P<0.05). The USS score, the frequency of urination, and the occurrence of urgent urination and urinary incontinence in both groups decreased compared with those before treatment, all showing statistically significant differences; moreover, the combination group had better improvements in USS score, the frequency of urination, and the occurrence of urgent urination and urinary incontinence than the control group, among which the USS score, the frequency of urination, and the occurrence of urinary incontinence in the combination group had statistically significant differences from those in the control group ( P<0.05). After treatment, the ICSI and VAS scores in both groups decreased, and the two scores in the combination group were significantly lower than those in the control group, with statistically significant differences ( P<0.05). Conclusion:Hyperbaric oxygen combined with transurethral electrocoagulation has a significant effect on hemorrhagic radiation cystitis, which can effectively improve the urodynamic properties of patients, reduce USS, OABSS, ICSI, and VAS scores, and promote the prognosis.