Objective To identify the characteristics of HCoV-OC43genotype in recent years.Methods Nucleic acids were extracted from HCoV-OC43-positive respiratory specimens collected from 2013 to 2015.The cDNA were generated by reverse transcription.Specific primers were used to amplify the full length of spike (S),RNA dependent RNA polymerase (RdRp) and Nucleocapsid (N) genes.The sequences assembling,phylogenic analyzing and amino acids alignments were performed by using Mega 6.0 software.Results The genes were successful amplified from 16 HCoV-OC43 clinical samples.Of the 16 strains,1 from 2013,all 8 from 2014 and 3 from 2015 belonged to genotype D,and 3 from 2015 belonged to genotype B,and 1 from 2015 belonged to genotype E named by China,E (CH).The coding polymorphisms were found in S protein,with 9 sites in B and 17 sites in D genotypes were between chronologically distinct strains.Accumulations of amino acids mutations were observed during the evolution of B genotype,while reverse and successive changes were found in D genotypes.Conclusions Multiple genotypes of HCoV-OC43 co-circulated,with dominant epidemic of D genotype.The genetic drift in S gene plays an important role in the epidemic of specific genotype of HCoV-OC43.Our results provide insights to clarify the molecular epidemic characteristics of HCoV-OC43.