Objective:To observe the effect of high-frequency repetitive transcranial magnetic stimulation (rTMS) at different frequencies on cognitive impairment due to high-altitude hypoxia.Methods:Sixty officers and soldiers displaying cognitive impairment in a hypoxic high-altitude environment were randomly divided into 15Hz, 20Hz and 25Hz groups, each of 20. They were given rTMS at those frequencies for 30 days. Before the stimulation and after 15 and 30 days, event-related potentials, latencies of mismatched negativity (MMN) and P300 signals were recorded. The participants′ cognition was also evaluated using the Montreal Cognitive Assessment Scale (MoCA). Correlation between the electrophysiological indexes and the MoCA scores was computed.Results:After 15 days, all had shorter MMN latencies, higher total MoCA scores and better memory scores. The only significant difference among the three groups was in the average memory scores. After 15 days, MMN latency was significantly negatively correlated with the memory scores in all three groups ( r=0.44 to -0.54). Conclusions:rTMS at frequencies above 15Hz can effectively relieve cognitive impairment, especially memory dysfunction, resulting from high-altitude hypoxia.

Objective To explore the practice of cost control and nursing quality improvement for obstetric and gyneco-logical inpatients based on the Diagnosis-Related Groups(DRGs)model.Methods A total of 208 inpatients admitted to the ob-stetrics and gynecology department of our hospital from June 1,2023,to December 31,2024,were selected as the study sub-jects.They were divided into a control group(104 cases,conventional payment model)and an observation group(104 cases,DRGs model)using a random number table method.The hospitalization costs(examination fees,medication fees,surgery fees,consumable costs,per capita total hospitalization costs),average length of stay,nursing quality,and inpatient satisfaction were compared between the two groups.Results In the observation group,the main cost components—examination fees,medication fees,surgery fees,consumable costs,and per capita total hospitalization costs—were all lower than those in the control group,with statistically significant differences(P＜0.05).The average length of stay in the observation group was shorter than that in the control group.The scores for safety management,nursing standards,ward management,and nursing quality in the observation group were higher than those in the control group,with statistically significant differences(P＜0.05).The inpatient nursing sat-isfaction rate in the observation group was 91.35％,significantly higher than that in the control group,with a statistically signifi-cant difference(P＜0.05).Conclusion The application of the DRGs model in obstetric and gynecological inpatients can effec-tively reduce related hospitalization costs and improve nursing quality and inpatient satisfaction.

Objective To explore the practice of cost control and nursing quality improvement for obstetric and gyneco-logical inpatients based on the Diagnosis-Related Groups(DRGs)model.Methods A total of 208 inpatients admitted to the ob-stetrics and gynecology department of our hospital from June 1,2023,to December 31,2024,were selected as the study sub-jects.They were divided into a control group(104 cases,conventional payment model)and an observation group(104 cases,DRGs model)using a random number table method.The hospitalization costs(examination fees,medication fees,surgery fees,consumable costs,per capita total hospitalization costs),average length of stay,nursing quality,and inpatient satisfaction were compared between the two groups.Results In the observation group,the main cost components—examination fees,medication fees,surgery fees,consumable costs,and per capita total hospitalization costs—were all lower than those in the control group,with statistically significant differences(P＜0.05).The average length of stay in the observation group was shorter than that in the control group.The scores for safety management,nursing standards,ward management,and nursing quality in the observation group were higher than those in the control group,with statistically significant differences(P＜0.05).The inpatient nursing sat-isfaction rate in the observation group was 91.35％,significantly higher than that in the control group,with a statistically signifi-cant difference(P＜0.05).Conclusion The application of the DRGs model in obstetric and gynecological inpatients can effec-tively reduce related hospitalization costs and improve nursing quality and inpatient satisfaction.

Objective:To observe the effect of high-frequency repetitive transcranial magnetic stimulation (rTMS) at different frequencies on cognitive impairment due to high-altitude hypoxia.Methods:Sixty officers and soldiers displaying cognitive impairment in a hypoxic high-altitude environment were randomly divided into 15Hz, 20Hz and 25Hz groups, each of 20. They were given rTMS at those frequencies for 30 days. Before the stimulation and after 15 and 30 days, event-related potentials, latencies of mismatched negativity (MMN) and P300 signals were recorded. The participants′ cognition was also evaluated using the Montreal Cognitive Assessment Scale (MoCA). Correlation between the electrophysiological indexes and the MoCA scores was computed.Results:After 15 days, all had shorter MMN latencies, higher total MoCA scores and better memory scores. The only significant difference among the three groups was in the average memory scores. After 15 days, MMN latency was significantly negatively correlated with the memory scores in all three groups ( r=0.44 to -0.54). Conclusions:rTMS at frequencies above 15Hz can effectively relieve cognitive impairment, especially memory dysfunction, resulting from high-altitude hypoxia.

BACKGROUND: Long-term remote ischemic conditioning (RIC) has been proven to be beneficial in multiple diseases, such as cerebral and cardiovascular diseases. However, the hyperacute and acute effects of a single RIC stimulus are still not clear. Quantitative proteomic analyses of plasma proteins following RIC application have been conducted in preclinical and clinical studies but exhibit high heterogeneity in results due to wide variations in experimental setups and sampling procedures. Hence, this study aimed to explore the immediate effects of RIC on plasma proteome in healthy young adults to exclude confounding factors of disease entity, such as medications and gender. METHODS: Young healthy male participants were enrolled after a systematic physical examination and 6-month lifestyle observation. Individual RIC sessions included five cycles of alternative ischemia and reperfusion, each lasting for 5 min in bilateral forearms. Blood samples were collected at baseline, 5 min after RIC, and 2 h after RIC, and then samples were processed for proteomic analysis using liquid chromatography-tandem mass spectrometry method. RESULTS: Proteins related to lipid metabolism (e.g., Apolipoprotein F), coagulation factors (hepatocyte growth factor activator preproprotein), members of complement cascades (mannan-binding lectin serine protease 1 isoform 2 precursor), and inflammatory responses (carboxypeptidase N catalytic chain precursor) were differentially altered at their serum levels following the RIC intervention. The most enriched pathways were protein glycosylation and complement/coagulation cascades. CONCLUSIONS One-time RIC stimulus may induce instant cellular responses like anti-inflammation, coagulation, and fibrinolysis balancing, and lipid metabolism regulation which are protective in different perspectives. Protective effects of single RIC in hyperacute and acute phases may be exploited in clinical emergency settings due to apparently beneficial alterations in plasma proteome profile. Furthermore, the beneficial effects of long-term (repeated) RIC interventions in preventing chronic cardiovascular diseases among general populations can also be expected based on our study findings.
Young Adult ; Humans ; Male ; Proteome ; Cardiovascular Diseases ; Proteomics ; Ischemia ; Blood Coagulation

Objective To study efficacy and safety of large dose of pamidronate disodium in treatment for patients with painful bone metastasis of prostate cancer. Methods A total of 100 patients with painful bone metastasis of prostate cancer were randomized into large dose group and conventional dose group, with 50 cases each. Pamidronate disodium was administered by intravenous infusion, 90 mg on the first day and 60 mg on the second day for large dose group, and 60 mg on the first day and 30 mg on the second day for conventional dose group, respectively, every 4 weeks for two courses. Changes of pain,mobility, quality of life and adverse effect in patients before and after treatment were observed. Results After treatment, pain was relieved in 43 of 50 patients ( 86% ) in large dose group, significantly more than that in conventional dose group (21/50, 42% ) (χ2 = 22.79, P ＜ 0. 01 ). Both mobility and quality of life were improved in 39 and 33 patients ( 87% and 66% ), respectively in large dose group and 21 and 18 (46% and 36% ), respectively in conventional dose group (χ2 = 17.04 and 9. 00, P ＜0. 01 ). No severe adverse effect in both groups was observed. Conclusions Pain in patients with bone metastasis of prostate cancer can be significantly relieved with large dose of pamidronate disodium, as well as their quality of life improved.