Objective To observe the value of traditional pelvic floor ultrasound parameters combined with middle urethral sphincter elasticity parameters for diagnosing female stress urinary incontinence(SUI).Methods Fifty two female SUI patients(SUI group)and 45 healthy women(control group)were prospectively enrolled.Traditional pelvic floor ultrasound parameters and middle urethral sphincter elasticity parameters were compared between groups,and logistic regression analysis was performed,the efficacy of each parameter alone and their combination for diagnosing SUI was analyzed.Results Significant differences of bladder neck descent(BND),urethral rotation angle(URA),posterior urethrovesical angle(PUA),shear modulus of the middle urethral anterior wall sphincter at rest state(Q1),shear modulus of the middle urethral anterior wall sphincter under maximum Valsalva maneuver(Q2),and shear modulus of the middle urethral posterior wall sphincter at resting-state(H1)were found between groups(all P＜0.05).BND,PUA,Q1 and Q2 were all influencing factors of female SUI(all P＜0.05),with the area under the curve(AUC)for diagnosing SUI of 0.721,0.718,0.659 and 0.288,respectively.Then traditional ultrasound model,elasticity ultrasound model and combined model were constructed based on traditional pelvic floor ultrasound parameters(BND,PUA),middle urethral sphincter elasticity parameters(Q1,Q2)and their combination,respectively,with AUC for diagnosing SUI of 0.837,0.754 and 0.908,respectively.The AUC of combined model was higher than that of traditional ultrasound model,elasticity ultrasound model and each ultrasound parameter alone(all P＜0.05).Conclusion Traditional pelvic floor ultrasound parameters combined with middle urethral sphincter elasticity parameters had high value for diagnosing female SUI.

Objective To observe the value of middle urethral motion and sphincter elasticity for diagnosing female stress urinary incontinence(SUI).Methods Totally 97 female patients,including 52 with SUI(SUI group)and 45 without SUI(control group)were prospectively enrolled.Pelvic floor ultrasound was performed under resting state and the maximum Valsalva maneuver,respectively,and bladder neck mobility(BND),upper-lower mobility of middle urethra(UMupper-lower),anterior-posterior mobility of middle urethra(UM anterior-posterior),elasticity parameter of the anterior wall of middle urethral sphincter(ΔEanterior wall),as well as elasticity parameter of the posterior wall of middle urethral sphincter(ΔEposterior wall)were measured.Patients'general data and the above ultrasound parameters were compared between groups,and the efficacy of them for diagnosing SUI was analyzed.Results Significant differences of BND,UM upper-lower,ΔEanterior wall and ΔEposterior wall,of also the proportion of lateral episiotomy history were found between groups(all P＜0.05).Among them,UMupper-lower,ΔEanterior wall and ΔEposterior wall were all correlated with female SUI(rs=0.231,-0.533,-0.428,all P＜0.05).The area under the curve(AUC)of UMupper-lower,ΔEanterior wall,ΔEposterior wall and their combination for diagnosing SUI was 0.634,0.820,0.748 and 0.867,respectively.The AUC of the combination was significantly higher than that of each parameter alone(all P＜0.001).Conclusion The combination of middle urethral motion and sphincter elasticity was helpful for diagnosing female SUI.

Objective To observe the value of traditional pelvic floor ultrasound parameters combined with middle urethral sphincter elasticity parameters for diagnosing female stress urinary incontinence(SUI).Methods Fifty two female SUI patients(SUI group)and 45 healthy women(control group)were prospectively enrolled.Traditional pelvic floor ultrasound parameters and middle urethral sphincter elasticity parameters were compared between groups,and logistic regression analysis was performed,the efficacy of each parameter alone and their combination for diagnosing SUI was analyzed.Results Significant differences of bladder neck descent(BND),urethral rotation angle(URA),posterior urethrovesical angle(PUA),shear modulus of the middle urethral anterior wall sphincter at rest state(Q1),shear modulus of the middle urethral anterior wall sphincter under maximum Valsalva maneuver(Q2),and shear modulus of the middle urethral posterior wall sphincter at resting-state(H1)were found between groups(all P＜0.05).BND,PUA,Q1 and Q2 were all influencing factors of female SUI(all P＜0.05),with the area under the curve(AUC)for diagnosing SUI of 0.721,0.718,0.659 and 0.288,respectively.Then traditional ultrasound model,elasticity ultrasound model and combined model were constructed based on traditional pelvic floor ultrasound parameters(BND,PUA),middle urethral sphincter elasticity parameters(Q1,Q2)and their combination,respectively,with AUC for diagnosing SUI of 0.837,0.754 and 0.908,respectively.The AUC of combined model was higher than that of traditional ultrasound model,elasticity ultrasound model and each ultrasound parameter alone(all P＜0.05).Conclusion Traditional pelvic floor ultrasound parameters combined with middle urethral sphincter elasticity parameters had high value for diagnosing female SUI.

Objective To observe the value of middle urethral motion and sphincter elasticity for diagnosing female stress urinary incontinence(SUI).Methods Totally 97 female patients,including 52 with SUI(SUI group)and 45 without SUI(control group)were prospectively enrolled.Pelvic floor ultrasound was performed under resting state and the maximum Valsalva maneuver,respectively,and bladder neck mobility(BND),upper-lower mobility of middle urethra(UMupper-lower),anterior-posterior mobility of middle urethra(UM anterior-posterior),elasticity parameter of the anterior wall of middle urethral sphincter(ΔEanterior wall),as well as elasticity parameter of the posterior wall of middle urethral sphincter(ΔEposterior wall)were measured.Patients'general data and the above ultrasound parameters were compared between groups,and the efficacy of them for diagnosing SUI was analyzed.Results Significant differences of BND,UM upper-lower,ΔEanterior wall and ΔEposterior wall,of also the proportion of lateral episiotomy history were found between groups(all P＜0.05).Among them,UMupper-lower,ΔEanterior wall and ΔEposterior wall were all correlated with female SUI(rs=0.231,-0.533,-0.428,all P＜0.05).The area under the curve(AUC)of UMupper-lower,ΔEanterior wall,ΔEposterior wall and their combination for diagnosing SUI was 0.634,0.820,0.748 and 0.867,respectively.The AUC of the combination was significantly higher than that of each parameter alone(all P＜0.001).Conclusion The combination of middle urethral motion and sphincter elasticity was helpful for diagnosing female SUI.

Objective:To investigate the effect of electroacupuncture treatment on the integrity of myelin and the related molecules in the spinal dorsal horn of neuropathic pain model rat.Methods:Totally 32 SPF grade Sprague-Dawley rats were randomly divided into 4 groups: sham group, pain model group, electroacupuncture group and sham electroacupuncture group, with 8 rats in each group. Neuropathic pain model was established by right sciatic nerve ligation. Electroacupuncture stimulation of Huantiao point and Yanglingquan point was started from 1 day after surgery, 30 min/d, and lasted for 14 days. The paw withdraw mechanical threshold (PWMT) were tested before surgery, 3 days, 7 days and 14 days after surgery. Immunofluorescence was used to detect myelin basic protein (MBP) in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn and Western blot was used to measure the expression of β-secreatase 1(BACE1), neuregulin 1 type Ⅲ (NRG1 Ⅲ) and phosphorylated ErbB receptor tyrosine kinase 2 (p-ErbB2) at 14 days after surgery.SPSS 24.0 was used for statistical analysis.Repeated measurement ANOVA was used for behavioral analysis and one-way ANOVA followed by Bonferroni test was used for Western blot and immunofluorescence data analysis.Results:(1) The results of pain behaviors showed that the interaction effect of time and group on PWMT was significant ( F=29.817, P<0.001), and both the time main effect ( F=240.598, P<0.001) and group main effect ( F=304.291, P<0.001) were significant. There were no significant differences among 4 groups before surgery. Compared with pain model group, the PWMT of electroacupuncture group were significantly increased at 3 days ((16.87±1.82) g, (11.31±1.36) g), 7 days ((15.09±1.75) g, (10.33±0.73) g) and 14 days ((15.07±1.49) g, (9.87±0.98) g) after surgery (all P<0.01). (2)The difference of the intensity of MBP in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn staining was significant among 4 groups ( F=92.06, P<0.001). Compared with sham group, the intensity of MBP staining in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn in pain model group was significantly decreased ((13.26±1.90)%, (36.37±0.68)%) ( P<0.01) ). Compared with pain model group, the intensity of MBP staining in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn in electroacupuncture group ((28.21±3.15)%) was significantly increased after electroacupuncture treatment ( P<0.01). (3) The differences of the expressions of BACE1, NRG1 Ⅲ and p-ErbB2 in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn were significant among the 4 groups ( F=31.04, 21.20, 11.74, all P<0.01). Compared with sham group, the expressions of BACE1, NRG1 Ⅲ and p-ErbB2 in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn in pain model group were significantly downregulated (BACE1 (0.42±0.09), (1.16±0.13); NRG1 Ⅲ (0.54±0.05), (1.00±0.10); p-ErbB2 (0.73±0.06), (1.02±0.15) ) (all P<0.05). Compared with pain model group, the expression of BACE1 (0.86±0.09), NRG1 Ⅲ (0.81±0.05) and p-ErbB2 (1.12±0.04) in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn in electroacupuncture group were upregulated(all P<0.05). Conclusion:Electroacupuncture treatment could relieve neuropathic pain, improve the demyelination and upregulate the expressions of myelin-related proteins in the ipsilateral Ⅰ-Ⅲ laminae of spinal dorsal horn.

Objective To analyze the postoperative therapeutic effect of prokinetic agents in patients after tricuspid valve replacement （TVR） under cardiopulmonary bypass. Methods Patients received TVR under cardiopulmonary bypass （during June 2010 to December 2021） in the department of Cardiovascular Surgery of the First Affiliated Hospital of Naval Medical University were selected as the subjects of our study. The data of basic characteristics, first postoperative defecation time and postoperative recovery condition were collected and retrospectively analyzed in the patients taking prokinetic agents within three days after surgery （prevention group） and patients not taking prokinetic agents within three days after surgery （control group）. Results A total of 184 patients were selected, including 101 in the prevention group and 83 in the control group. The first defecation time of patients in the prevention group was significantly earlier than that in the control group （P<0.05）. The incidences of abdominal distension and pulmonary infection in the prevention group were significantly lower than that in the control group. The length of time in ICU, postoperative mechanical ventilation and nasogastric tube decompression in the prevention group were significantly shorter than that in the control group （P<0.05）. Conclusion Taking prokinetic agents within three days after TVR under cardiopulmonary bypass could effectively improve the prognosis of patients.