OBJECTIVE To provide a reference for anticoagulant therapy and vaginal bleeding management during medical abortion in patients with missed abortion after aortic mechanical valve replacement surgery. METHODS Clinical pharmacists conducted pharmaceutical monitoring on a patient with missed abortion after aortic mechanical valve replacement surgery， and analyzed the correlation between warfarin use and abortion. In response to gingival bleeding and an increase in the international normalized ratio （INR） after taking mifepristone， clinical pharmacists recommended discontinuing warfarin based on bleeding risk and INR changes； for the selection of sex hormones after missed abortion， clinical pharmacists suggested that estradiol gel combined with progesterone should be used in sequential treatment， supplemented by Xian yimucao capsules， to accelerate the elimination of residual tissue and congestion in uterine cavity. RESULTS The physician followed the advice of the clinical pharmacist and did not prolong the patient’s hospital stay due to excessive vaginal bleeding during treatment. Warfarin treatment was restarted on the day of discharge， and INR remained stable within the target anticoagulation range after discharge without any adverse events such as thromboembolism. CONCLUSIONS In patients with missed abortion after aortic valve replacement surgery， mifepristone may enhance the anticoagulant effect of warfarin and increase the risk of bleeding during medical abortion. It is recommended to discontinue warfarin before medication. Clinical pharmacists play a crucial role in the development of anticoagulation plans， identification of drug-drug interactions， assessment of bleeding risks， and selection of postoperative sex hormones. Through full pharmaceutical monitoring， they ensure the safety of patients’ medication.

OBJECTIVE To provide a reference for anticoagulant therapy and vaginal bleeding management during medical abortion in patients with missed abortion after aortic mechanical valve replacement surgery. METHODS Clinical pharmacists conducted pharmaceutical monitoring on a patient with missed abortion after aortic mechanical valve replacement surgery， and analyzed the correlation between warfarin use and abortion. In response to gingival bleeding and an increase in the international normalized ratio （INR） after taking mifepristone， clinical pharmacists recommended discontinuing warfarin based on bleeding risk and INR changes； for the selection of sex hormones after missed abortion， clinical pharmacists suggested that estradiol gel combined with progesterone should be used in sequential treatment， supplemented by Xian yimucao capsules， to accelerate the elimination of residual tissue and congestion in uterine cavity. RESULTS The physician followed the advice of the clinical pharmacist and did not prolong the patient’s hospital stay due to excessive vaginal bleeding during treatment. Warfarin treatment was restarted on the day of discharge， and INR remained stable within the target anticoagulation range after discharge without any adverse events such as thromboembolism. CONCLUSIONS In patients with missed abortion after aortic valve replacement surgery， mifepristone may enhance the anticoagulant effect of warfarin and increase the risk of bleeding during medical abortion. It is recommended to discontinue warfarin before medication. Clinical pharmacists play a crucial role in the development of anticoagulation plans， identification of drug-drug interactions， assessment of bleeding risks， and selection of postoperative sex hormones. Through full pharmaceutical monitoring， they ensure the safety of patients’ medication.

Objective To explore the feasibility of confirming pre experimental conditions in Mongolian gerbils using three-dimensional time-of-flight magnetic resonance angiography(3D-TOF-MRA).Methods The cerebral arteries in Monglian gerbils were imaged using a 7.0 T magnetic resonance imaging scanner and the data were processed using RadiAnt DICOM Viewer software.The imaging result were then validated and compared using the latex perfusion method,and 3D reconstruction of the posterior communicating branch and surrounding cerebral blood vessels were performed using analytical software.Finally,we attempted to use this method to screen for abnormal vascular development in Mongolian gerbils,to verify the effectiveness of this method.Results 3D-TOF-MRA effectively demonstrated the cerebral arteries in live Mongolian gerbils.The technique showed high accuracy for observing the main cerebral arteries in Mongolian gerbils,but its ability to show finer vascular branches was not as good as the latex perfusion method.The data obtained using 3D-TOF-MRA could be used for 3D reconstruction of blood vessels,and the technology could be applied to screen for abnormal arterial structures in Mongolian gerbils.Conclusions 3D-TOF-MRA technology can be applied for the structural observation and related research of the cerebral arteries in live Mongolian gerbils.

Objective To explore the feasibility of confirming pre experimental conditions in Mongolian gerbils using three-dimensional time-of-flight magnetic resonance angiography(3D-TOF-MRA).Methods The cerebral arteries in Monglian gerbils were imaged using a 7.0 T magnetic resonance imaging scanner and the data were processed using RadiAnt DICOM Viewer software.The imaging result were then validated and compared using the latex perfusion method,and 3D reconstruction of the posterior communicating branch and surrounding cerebral blood vessels were performed using analytical software.Finally,we attempted to use this method to screen for abnormal vascular development in Mongolian gerbils,to verify the effectiveness of this method.Results 3D-TOF-MRA effectively demonstrated the cerebral arteries in live Mongolian gerbils.The technique showed high accuracy for observing the main cerebral arteries in Mongolian gerbils,but its ability to show finer vascular branches was not as good as the latex perfusion method.The data obtained using 3D-TOF-MRA could be used for 3D reconstruction of blood vessels,and the technology could be applied to screen for abnormal arterial structures in Mongolian gerbils.Conclusions 3D-TOF-MRA technology can be applied for the structural observation and related research of the cerebral arteries in live Mongolian gerbils.

Objective To investigate the effect of restraint stress on liver injury in mice induced by D-galactosamine and lipopolysaccharide (D+L).Methods Normal BALB/c (B/c) mice were randomly divided into normal control, stress control, D+L group, and D+L+stress group.The mice of normal control group were bred routinely.The stress group was giv-en stress regularly and quantitatively.Mice in the D+L group were injected intraperitoneally with mixed solution of D-galac-tosamine and lipopolysaccharide at final concentration of 30 mg/mL and 2μg/mL, respectively, once every two days.The D+L+stress group was given equal stress as stress group after injection of D-galactosamine and lipopolysaccharide mixed solution. Eight weeks later, blood samples were collected to test serum aminotransferase (ALT) and aspartate aminotransferase (AST), liver tissue samples from all animals were collected to evaluate the degree of liver fibrosis by HE and Masson staining.Results At the 8th week, the ALT and AST values in the D+L+stress group were significantly reduced( P＜0.01) and AST/ALT value was significantly increased(P＜0.01)compared with that in the D+L group.For HE and Masson staining, disordered structure of hepatic lobules, nodular hyperplasia, and necrosis of epithelial cells were present in animals of the D+L group.However, no obvious pathological changes were observewd in the D+L+stress group.For fibrosis scores, the fibrosis grade in the D+L+stress group was significantly decreased than that of the D+L group (P＜0.05).Conclusions Constraint stress presents pro-tective effect on D-galactosamine and lipopolysaccharide induced liver injury in mice.