1.Scientific characterization of medicinal amber: evidence from geological and archaeological studies.
Qi LIU ; Qing-Hui LI ; Di-Ying HUANG ; Yan LI ; Pan XIAO ; Ji-Qing BAI ; Hua-Sheng PENG ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2025;50(11):2905-2914
Amber and subfossil resins are subjects of interdisciplinary research across multiple fields. However, due to their diverse origins and complex compositions, different disciplines vary in their definitions and functional interpretations. In traditional Chinese medicine(TCM), amber has been utilized as a medicinal material since ancient time, with extensive historical documentation. However, its classification, provenance, and nomenclature remain ambiguous, and authentic medicinal amber artifacts are exceedingly rare. This study employed Fourier-transform infrared spectroscopy(FTIR) to characterize amber and subfossil resins from various geological sources and commercially "medicinal amber". Additionally, historical literature and market surveys were analyzed to explore their provenance, composition, and functional attributes. The results indicate that amber and subfossil resins from different sources and with different compositions exhibit distinct fingerprint characteristics in the FTIR spectral range of 1 800-700 cm~(-1). "Medicinal amber" available in the market primarily consists of subfossil or modern resins, significantly differing in composition and structure from geological amber. This study highlights the importance of interdisciplinary research on amber identification and resource management. It is essential to establish a systematic database of amber and subfossil resin characteristics and integrate modern analytical techniques to enhance research on their composition, pharmacological mechanisms, and potential therapeutic effects, thereby promoting the standardized utilization of amber resources and advancing the modernization of TCM.
Amber/history*
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Archaeology
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Spectroscopy, Fourier Transform Infrared
;
Medicine, Chinese Traditional
2.Scientific analysis and usage reassessment of suspected medicinal cinnabar unearthed from Mawangdui Tomb No.3 of the Han Dynasty.
Ning-Ning XU ; Ting-Yan REN ; Ming-Jie LI ; Pan XIAO ; Guo-Hui SHEN ; Ji-Qing BAI ; Qi LIU
China Journal of Chinese Materia Medica 2025;50(11):2915-2923
Cinnabar(HgS) was widely used in ancient times for medicinal purposes, religious rituals, and pigments. A group of bright red powdery clumps was excavated from Mawangdui Tomb No.3 of the Han Dynasty. Early studies considered the clumps as evidence of cinnabar's medicinal use during the Qin-Han period. This study employed a range of archaeometric techniques, including extended-depth-of-field stereo imaging, micro-CT, scanning electron microscopy-energy dispersive spectroscopy, Raman spectroscopy, and Fourier transform infrared spectrometry FTIR, to systematically analyze the material composition and structural characteristics of these remains. The results revealed that the cinnabar particles were granular, finely ground, and tightly bound to silk matrix, with no detectable excipients typically associated with medicinal formulations. Micro-CT imaging indicated a well-preserved textile structure, with clear signs of sedimentary accumulation and mechanical damage. Based on historical and archaeological studies, this study suggested that these remains were more likely degraded accumulations of cinnabar-colored silk textiles rather than medicinal cinnabar. By clarifying the diversity of ancient cinnabar applications and preservation states, this study provides new insights for the archaeological identification of mineral medicinal materials and contributes to the standardized study of Chinese medicinal materials and understanding of the historical use of cinnabar.
History, Ancient
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China
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Humans
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Medicine, Chinese Traditional/history*
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Archaeology
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Drugs, Chinese Herbal/history*
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Spectroscopy, Fourier Transform Infrared
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Spectrum Analysis, Raman
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Mercury Compounds
3.Pinworm Infection at Salmon Ruins and Aztec Ruins: Relation to Pueblo III Regional Violence
Karl J REINHARD ; Morgana CAMACHO
The Korean Journal of Parasitology 2019;57(6):627-633
The study of coprolites has been a theme of archaeology in the American Southwest. A feature of archaeoparasitology on the Colorado Plateau is the ubiquity of pinworm infection. As a crowd parasite, this ubiquity signals varying concentrations of populations. Our recent analysis of coprolite deposits from 2 sites revealed the highest prevalence of infection ever recorded for the region. For Salmon Ruins, the deposits date from AD 1140 to 1280. For Aztec Ruins, the samples can be dated by artifact association between AD 1182–1253. Both sites can be placed in the Ancestral Pueblo III occupation (AD 1100–1300), which included a period of cultural stress associated with warfare. Although neither of these sites show evidence of warfare, they are typical of large, defensible towns that survived this time of threat by virtue of large populations in stonewalled villages with easily accessible water. We hypothesize that the concentration of large numbers of people promoted pinworm infection and, therefore, explains the phenomenal levels of infection at these sites.
Archaeology
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Artifacts
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Colorado
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Enterobius
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Occupations
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Parasites
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Prevalence
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Salmon
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Violence
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Virtues
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Warfare
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Water
4.Confusing a Pollen Grain with a Parasite Egg: an Appraisal of “Paleoparasitological Evidence of Pinworm (Enterobius Vermicularis) Infection in a Female Adolescent Residing in Ancient Tehran”
Morgana CAMACHO ; Karl J REINHARD
The Korean Journal of Parasitology 2019;57(6):621-625
There is often the risk of confusing pollen grains with helminth eggs from archaeological sites. Thousands to millions of pollen grains can be recovered from archaeological burial sediments that represent past ritual, medication and environment. Some pollen grain types can be similar to parasite eggs. Such a confusion is represented by the diagnosis of enterobiasis in ancient Iran. The authors of this study confused a joint-pine (Ephedra spp.) pollen grain with a pinworm egg. This paper describes the specific Ephedra pollen morphology that can be confused with pinworm eggs.
Adolescent
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Archaeology
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Burial
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Diagnosis
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Eggs
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Enterobiasis
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Enterobius
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Ephedra
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Female
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Helminths
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Humans
;
Iran
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Ovum
;
Parasites
;
Pollen
5.Archaeoparasitological Analysis of Samples from the Cultural Layer of Nadym Gorodok dated Back to the 14th-Late 18th Centuries
Sergey Mikhailovich SLEPCHENKO ; Oleg Viktorovich KARDASH ; Vyacheslav Sergeyevich SLAVINSKY ; Sergey Nikolaevich IVANOV ; Rakultseva Daria SERGEYEVNA ; Alexander Alekseevich TSYBANKOV ; Dong Hoon SHIN
The Korean Journal of Parasitology 2019;57(6):567-573
An archaeoparasitological analysis of the soil samples from Nadym Gorodok site of Western Siberia has been carried out in this study. The archaeological site was dated as the 13 to 18th century, being characterized as permafrost region ensuring good preservation of ancient parasite eggs. Parasite eggs as Opisthorchis felineus, Alaria alata, and Diphyllobothrium sp. were found in the archaeological soil samples, which made clear about the detailed aspects of Nadym Gorodok people's life. We found the Diphyllobothrium sp. eggs throughout the 14 to 18th century specimens, allowing us to presume that raw or undercooked fish might have been commonly used for the foods of Nadym Gorodok inhabitants and their dogs for at least the past 400 years. Our study on Nadym Gorodok specimens also demonstrate that there might have been migratory interactions and strong economic ties between the people and society in Western Siberia, based on archaeoparasitological results of Opisthorchis felineus in Western Siberia.
Animals
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Archaeology
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Asia, Northern
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Diphyllobothrium
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Dogs
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Eggs
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Humans
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Middle Aged
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Opisthorchis
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Ovum
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Parasites
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Permafrost
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Siberia
;
Soil
6.Radiocarbon Dating of Skeletal Remains: Case Report
Jong Pil PARK ; Seung Gyu CHOI ; Sang Seob LEE ; Won Joon LEE ; Jeong Uk SEO ; Chang Un CHOI ; Yi Suk KIM ; U Young LEE ; Minsung CHOI ; Kyung Moo YANG
Korean Journal of Legal Medicine 2018;42(3):105-109
While radioactive isotope analysis has proved to be a useful method in disciplines such as archaeology and forensic anthropology, more recently, radiocarbon dating has allowed for a more nuanced biological profile of human skeletal remains. Radiocarbon dating has been made possible by the above ground nuclear bomb test conducted in 1963, which raised the level of atmospheric radiocarbon concentration to almost twice the natural level. Because the annually measured tropospheric ¹⁴C concentrations are integrated into the bomb peak curve, the time of birth and death of an individual can be estimated by comparing the radiocarbon content of a skeletal sample to the bomb-curve value. In July 2017, about 1,000 skeletal remains were excavated at the construction site of Sokcho. For medico-legal purposes, we conducted anthropological and odontological examinations of all the human remains. We then conducted the radiocarbon analysis on seven femora (head and body portions), five mandibular teeth, and soil from the site through a request to the Korea Institute of Geoscience and Mineral Resources. The results demonstrated that the estimated year of birth or death was prior to the 1950s. Due to the diverse distribution of results, we deduced that the human remains were from the local mass grave. This study supports and suggests the use of radiocarbon dating more frequently in the analysis of human skeletal remains.
Archaeology
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Bombs
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Earth Sciences
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Forensic Anthropology
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Gangwon-do
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Humans
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Korea
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Methods
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Miners
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Parturition
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Radiometric Dating
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Soil
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Tooth
7.Prehistoric Pathoecology as Represented by Parasites of a Mummy from the Peruaçu Valley, Brazil.
Karl J REINHARD ; Adauto ARAÚJO
The Korean Journal of Parasitology 2016;54(5):585-590
Paleopathologists have begun exploring the pathoecology of parasitic diseases in relation to diet and environment. We are summarizing the parasitological findings from a mummy in the site of Lapa do Boquete, a Brazilian cave in the state of Minas Gerais. These findings in context of the archaeology of the site provided insights into the pathoecology of disease transmission in cave and rockshelter environments. We are presenting a description of the site followed by the evidence of hookworm, intestinal fluke, and Trypanosoma infection with resulting Chagas disease in the mummy discovered in the cave. These findings are used to reconstruct the transmission ecology of the site.
Ancylostomatoidea
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Archaeology
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Brazil*
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Chagas Disease
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Diet
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Echinostoma
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Ecology
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Mummies*
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Parasites*
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Parasitic Diseases
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Trematoda
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Trypanosoma
8.Ancient Human Parasites in Ethnic Chinese Populations.
Hui Yuan YEH ; Piers D MITCHELL
The Korean Journal of Parasitology 2016;54(5):565-572
Whilst archaeological evidence for many aspects of life in ancient China is well studied, there has been much less interest in ancient infectious diseases, such as intestinal parasites in past Chinese populations. Here, we bring together evidence from mummies, ancient latrines, and pelvic soil from burials, dating from the Neolithic Period to the Qing Dynasty, in order to better understand the health of the past inhabitants of China and the diseases endemic in the region. Seven species of intestinal parasite have been identified, namely roundworm, whipworm, Chinese liver fluke, oriental schistosome, pinworm, Taenia sp. tapeworm, and the intestinal fluke Fasciolopsis buski. It was found that in the past, roundworm, whipworm, and Chinese liver fluke appear to have been much more common than the other species. While roundworm and whipworm remained common into the late 20th century, Chinese liver fluke seems to have undergone a marked decline in its prevalence over time. The iconic transport route known as the Silk Road has been shown to have acted as a vector for the transmission of ancient diseases, highlighted by the discovery of Chinese liver fluke in a 2,000 year-old relay station in northwest China, 1,500 km outside its endemic range.
Archaeology
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Asian Continental Ancestry Group*
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Burial
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Cestoda
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China
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Clonorchis sinensis
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Communicable Diseases
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Enterobius
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Fasciola hepatica
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Fasciolidae
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Helminths
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Humans*
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Mummies
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Parasites*
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Prevalence
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Schistosoma japonicum
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Silk
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Soil
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Taenia
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Toilet Facilities
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Trematoda
9.Human Skeletal Remains from Ancient Burial Sites in India: With Special Reference to Harappan Civilization.
Astha DIBYOPAMA ; Yong Jun KIM ; Chang Seok OH ; Dong Hoon SHIN ; Vasant SHINDE
Korean Journal of Physical Anthropology 2015;28(1):1-9
Harappan Civilization is well known for highly sophisticated urban society, having been flourished in extensive regions of northwestern part of Pakistan and northeastern part of Afghanistan as its heyday around 4500 years ago. Most archaeologists agree on the periodization of this civilization as three different phases (Early, Mature and Late), which represent its cultural process of origin, development and decline. From the Harappan sites, one can note that there were about more than fifty burial sites discovered so far related with the civilization. In this article, we are trying to introduce the brief picture of the Harappan burials from the archaeological as well as anthropological perspectives.
Afghanistan
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Anthropology
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Archaeology
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Burial*
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Civilization*
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Humans
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India*
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Pakistan
10.V-shaped Pits in Regions of Ancient Baekje Kingdom Paleoparasitologically Confirmed as Likely Human-Waste Reservoirs.
Dong Hoon SHIN ; Sang Yuck SHIM ; Myeung Ju KIM ; Chang Seok OH ; Mi Hyun LEE ; Suk Bae JUNG ; Geon Il LEE ; Jong Yil CHAI ; Min SEO
The Korean Journal of Parasitology 2014;52(5):569-573
In a paleo-parasitological analysis of soil samples obtained from V-shaped pits dating to the ancient Baekje period in Korean history, we discovered Ascaris lumbricoides, Trichuris trichiura, and Clonorchis sinensis eggs. In light of the samples' seriously contaminated state, the V-shaped pits might have served as toilets, cesspits, or dung heaps. For a long period of time, researchers scouring archaeological sites in Korea have had difficulties locating such structures. In this context then, the present report is unique because similar kind of the ancient ruins must become an ideal resource for successful sampling in our forthcoming paleoparasitological studies.
Animals
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*Archaeology
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Helminths/classification/*isolation & purification
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Humans
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Ovum/*classification
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Parasitology
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Republic of Korea
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*Sanitary Engineering
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Soil/*parasitology

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