Obejective To explore whether it is possible to detect the 123I-prostate-specific membrane antigen(PSMA)radiation value of the puncture tissue during prostate biopsy to achieve real-time,rapid,and accurate identification of benign and malignant prostate tissues,so as to improve the current clinical biopsy strategy and achieve accurate diagnosis of prostate cancer during operation with fewer puncture needles.Method In this prospective,diagnostic trial,we included 29 patients with suspected prostate cancer.All patients underwent transperineal biopsy guided by ultrasound within 24 hours after injection of 123I-PSMA,a total of 435 punctures were performed.The radiation value of punctured tissue was measured in real-time with a gamma counter.Pearson test is used to correlate radiation value with histopathology.Result The median radiation value of prostate cancer tissue(1 906.50 cpm)was significantly higher than that of benign prostate tissue(415.00 cpm).The optimal cut-off value for distinguishing benign and malignant prostate tissues was 828.50 cpm.The median radiation value of clinically significant prostate cancer tissue(2 652.50 cpm)was significantly higher than that of clinically insignificant prostate cancer(1 386.00 cpm).The optimal cut-off value for distinguishing clinically significant and clinically insignificant prostate cancer tissues was 1 767.00 cpm.In additional,there was a significant positive correlation between the radiation value of puncture tissue and ISUP pathological grade(r=0.834).Conclusion It is preliminarily confirmed that detection of 123I-PSMA radiation value of prostate puncture tissue can realize real-time,rapid and accurate identification of benign and malignant prostate tissues during operation.

Objective:To compare the lung shunt fraction (LSF) of 90Y imaging after 90Y-selective internal radiation therapy (SIRT) and preoperative 99Tc m-macroaggregated albumin (MAA) imaging in patients with liver malignant tumors, and compare the volume and visual score of intrahepatic distribution of both nucleins on SPECT/CT images. Methods:A total of 91 patients with liver malignant tumors (78 males, 13 females; age (56.7±13.7)years; 99 cases) who underwent 90Y-SIRT in the Second Affiliated Hospital of Guangzhou Medical University from November 2022 to June 2024 were retrospectively collected. All patients underwent preoperative 99Tc m-MAA simulation and postoperative 90Y distribution verification by whole-body planar scintigraphy and hepatic SPECT/CT imaging. ROIs of the liver and lungs under the anterior-posterior position were delineated on the planar scintigraphy and LSF of 99Tc m-MAA and 90Y were calculated. The volume of interest (VOI) was drawn on the SPECT/CT images to calculate the nuclide distribution volume of both 99Tc m-MAA and 90Y within the liver. Wilcoxon signed rank test was used to compare the difference between two groups. In addition, the liver was divided into five lobes, namely left lateral lobe, left medial lobe, caudate lobe, right anterior lobe and right posterior lobe. Visual assessment of 90Y and 99Tc m-MAA radioactive distribution was performed ( 90Y and 99Tc m-MAA uptakes were graded on a scale of 0-3, where 0 indicated no nuclide accumulation and 3 indicated heavy accumulation). Kappa consistency test was used to analyze the scores of the corresponding lobes between two groups. Results:LSF for 99Tc m-MAA was 11.60%(4.27%, 15.03%), and LSF for 90Y was 11.80%(9.70%, 13.30%), without significant difference ( Z=-1.50, P=0.134). The distribution volume of 99Tc m-MAA within the liver was 542.63(204.00, 818.00)ml, which was significantly different from that of 90Y (688.69(287.00, 954.00)ml; Z=-7.37, P<0.001). Kappa values of the score of each lobe between 99Tc m-MAA imaging and 90Y imaging were 0.469-0.740 (all P<0.001). Conclusions:99Tc m-MAA simulation is reliable for assessing LSF for 90Y-SIRT. The distribution volume of 99Tc m-MAA is generally smaller than that of 90Y, but the consistency of the visual score of radioactive distribution is high. Overall, 99Tc m-MAA may well simulate the distribution pattern of 90Y-SIRT.

Obejective To explore whether it is possible to detect the 123I-prostate-specific membrane antigen(PSMA)radiation value of the puncture tissue during prostate biopsy to achieve real-time,rapid,and accurate identification of benign and malignant prostate tissues,so as to improve the current clinical biopsy strategy and achieve accurate diagnosis of prostate cancer during operation with fewer puncture needles.Method In this prospective,diagnostic trial,we included 29 patients with suspected prostate cancer.All patients underwent transperineal biopsy guided by ultrasound within 24 hours after injection of 123I-PSMA,a total of 435 punctures were performed.The radiation value of punctured tissue was measured in real-time with a gamma counter.Pearson test is used to correlate radiation value with histopathology.Result The median radiation value of prostate cancer tissue(1 906.50 cpm)was significantly higher than that of benign prostate tissue(415.00 cpm).The optimal cut-off value for distinguishing benign and malignant prostate tissues was 828.50 cpm.The median radiation value of clinically significant prostate cancer tissue(2 652.50 cpm)was significantly higher than that of clinically insignificant prostate cancer(1 386.00 cpm).The optimal cut-off value for distinguishing clinically significant and clinically insignificant prostate cancer tissues was 1 767.00 cpm.In additional,there was a significant positive correlation between the radiation value of puncture tissue and ISUP pathological grade(r=0.834).Conclusion It is preliminarily confirmed that detection of 123I-PSMA radiation value of prostate puncture tissue can realize real-time,rapid and accurate identification of benign and malignant prostate tissues during operation.

Objective:To compare the lung shunt fraction (LSF) of 90Y imaging after 90Y-selective internal radiation therapy (SIRT) and preoperative 99Tc m-macroaggregated albumin (MAA) imaging in patients with liver malignant tumors, and compare the volume and visual score of intrahepatic distribution of both nucleins on SPECT/CT images. Methods:A total of 91 patients with liver malignant tumors (78 males, 13 females; age (56.7±13.7)years; 99 cases) who underwent 90Y-SIRT in the Second Affiliated Hospital of Guangzhou Medical University from November 2022 to June 2024 were retrospectively collected. All patients underwent preoperative 99Tc m-MAA simulation and postoperative 90Y distribution verification by whole-body planar scintigraphy and hepatic SPECT/CT imaging. ROIs of the liver and lungs under the anterior-posterior position were delineated on the planar scintigraphy and LSF of 99Tc m-MAA and 90Y were calculated. The volume of interest (VOI) was drawn on the SPECT/CT images to calculate the nuclide distribution volume of both 99Tc m-MAA and 90Y within the liver. Wilcoxon signed rank test was used to compare the difference between two groups. In addition, the liver was divided into five lobes, namely left lateral lobe, left medial lobe, caudate lobe, right anterior lobe and right posterior lobe. Visual assessment of 90Y and 99Tc m-MAA radioactive distribution was performed ( 90Y and 99Tc m-MAA uptakes were graded on a scale of 0-3, where 0 indicated no nuclide accumulation and 3 indicated heavy accumulation). Kappa consistency test was used to analyze the scores of the corresponding lobes between two groups. Results:LSF for 99Tc m-MAA was 11.60%(4.27%, 15.03%), and LSF for 90Y was 11.80%(9.70%, 13.30%), without significant difference ( Z=-1.50, P=0.134). The distribution volume of 99Tc m-MAA within the liver was 542.63(204.00, 818.00)ml, which was significantly different from that of 90Y (688.69(287.00, 954.00)ml; Z=-7.37, P<0.001). Kappa values of the score of each lobe between 99Tc m-MAA imaging and 90Y imaging were 0.469-0.740 (all P<0.001). Conclusions:99Tc m-MAA simulation is reliable for assessing LSF for 90Y-SIRT. The distribution volume of 99Tc m-MAA is generally smaller than that of 90Y, but the consistency of the visual score of radioactive distribution is high. Overall, 99Tc m-MAA may well simulate the distribution pattern of 90Y-SIRT.

Posttraumatic stress disorder(PTSD)is a delayed and persistent mental disorder caused by severe psychological trauma,and the prevalence of PTSD is gradually increasing.This article summarizes the epidemiology,causes and pathogenesis,diagnosis,treatment,future and prospect of PTSD,in order to provide reference for the prevention,diagnosis and treatment of PTSD.

Professor ZHOU Peng has deeply discussed the pathological characteristics of psoriasis vulgaris,emphasizing that the disease is usually manifested deficiency interweaved with excess,leading to frequent recurrence and persistent refractory,which may lead to psychological and emotional problems of patients.This paper further expounds the effect of blood stasis on the pathogenesis,progression and prognosis of psoriasis,and puts forward a new method of combining Lingnan fire needling and filiform needling acupuncture technique to treat psoriasis vulgaris with blood stasis syndrome.Professor ZHOU Peng believes that the treatment principle of this disease is"regulating the mind first,rectifying blood as a base,syndrome differentiating and eliminating pathogenic factors",aiming at comprehensively considering the etiology and symptoms,in order to achieve more effective treatment results.Combined with the analysis of dermoscopic signs,it provides a possible improvement direction for the treatment of psoriasis vulgaris from a new perspective.

Objective To study the anti-inflammatory and analgesic effects of Ski protein overexpression on writing in mice induced by acetic acid.Methods Eight-week-old male ICR mice were administered 0.7%acetic acid solution(0.1 mL/10 g)to induce a writhing reaction.The mice were divided into sham,acetic acid,acetic acid+ibuprofen,acetic acid+ad-EGFP,acetic acid+ad-ski-1,acetic acid+ad-ski-2,and acetic acid+sulfasalazine groups(n=10 mice per group).The time to the first appearance of twisting and the number of twists within 15 min were recorded.Small intestine tissues were removed to identify the effect of adenovirus transfection and to detect protein expression levels of pro-inflammatory factors and pain biomarkers and protein expression of nuclear factor(NF)-κB p65 and its binding with Ski protein.Results Ski protein was successfully overexpressed in small intestine after intraperitoneal injection of Ad-ski adenovirus.Overexpressed Ski protein delayed the start and decreased the frequency of writhing,comparable to ibuprofen(P＞0.05).Groups in which ski protein was overexpressed showed significantly inhibited protein expression of pro-inflammatory factors and pain biomarkers compared with the acetic acid group(P＜0.05).Moreover,NF-κB p65 formed complexes with Ski.Conclusions Overexpression of Ski protein has anti-inflammatory and analgesic effects on acetic acid-induced inflammatory pain by inhibiting the expression of inflammatory factors and pain biomarkers,via regulation of the NF-κB signaling pathway.

The working principle of TMC BC ROBO 6 intelligent blood collection system was described in brief.The causes of three faults during daily operation of the system were analyzed,and the countermeasures were put forward accordingly.References were provided for clinical engineers to treat similar faults.[Chinese Medical Equipment Journal,2024,45(6):113-116]

Objective:To investigate the clinical and chest computed tomography (CT) findings of children with drug-resistant pulmonary tuberculosis (DR-PTB) and drug-sensitive pulmonary tuberculosis (DS-PTB) in Sichuan region.Methods:Active pulmonary tuberculosis children with positive sputum Mycobacterium tuberculosis culture were collected and divided into the drug-resistant and drug-sensitive groups according to the results of the drug susceptibility test who admitted to the Public Health Clinical Center of Chengdu from January 2020 to October 2024. The general data such as age, ethnic, gender, initial or retreatment regimens, body mass index (BMI), clinical symptoms such as cough, sputum, fever, and chest CT features were compared between the two groups. Non-parametric rank sum test, chi-square test or Fisher′s exact probability method were used for comparison between groups. The CT risk factors for the development of DR-PTB were analyzed using binary logistic regression. Results:A total of 110 children were enrolled, including 44(40.00%) males and 66(60.00%) females, with the age of 13.00(12.00, 14.00) years (range from 5.00 to 14.00 years). There were 28 cases in drug-resistant group and 82 in drug-sensitive group. Comparison of the clinical data between the two groups showed that the percentages of retreated patients (25.00%(7/28)) and ethnic minorities (78.57%(22/28)) in the drug-resistant group were higher than those in the drug-sensitive group (1.22%(1/82) and 57.32%(47/82)), which were both statistically significant (Fisher′s exact probability method and χ2=4.03, respectively, both P<0.05). The age of the children in the drug-resistant group was 13.00 (12.00, 13.50) years, which was younger than the age of 13.00 (12.00, 14.00) years in the drug-sensitive group ( U=828.00, P=0.021). There were no statistically differences of gender, BMI, history of bacillus Calmette-Guérin (BCG) vaccination, history of exposure to tuberculosis patients, positive immunological diagnostic tests, and the incidences of cough, sputum, fever, wheezing, shortness of breath and chest pain between the two groups (all P>0.05). Chest CT signs showed that lung lesions involving both right and left lobes accounted for 53.57%(15/28) in the drug-resistant group, and the incidence of solid pulmonary parenchyma (including wall-less cavities) was 92.86%(26/28), which were both higher than those in the drug-sensitive group (15.85%(13/82) and 70.73%(58/82)), and the differences were statistically significant ( χ2=6.95 and 5.66, respectively, both P<0.05). The incidence of small nodules/tree-in-bud pattern in the drug-sensitive group was higher than that in the drug-resistant group (98.78%(81/82) vs 82.14%(23/28)), and the difference was statistically significant (Fisher′s exact probability method, P=0.004). Binary logistic regression showed that bilateral lung involvement and solid pulmonary parenchyma (including wall-less cavities) were positively correlated with DR-PTB in children (odds ratio ( OR)=4.487, 95% confidence internal ( CI) 1.561 to 12.897, P=0.005 and OR=9.670, 95% CI 1.626 to 57.529, P=0.013, respectively). The occurence of small nodules/tree-in-bud was negatively correlated with DR-PTB in children ( OR=0.058, 95% CI 0.006 to 0.584, P=0.016). Conclusions:Among pulmonary tuberculosis children in Sichuan region, ethnic minorities and retreatment patients with DR-PTB are more common than thoses with DS-PTB. Chest CT findings of DR-PTB mainly show multiple solid pulmonary parenchyma with wall-less cavities, and the lesions often involve right and left lung lobes, whereas DS-PTB CT findings mainly show small nodules/tree-in-bud predominantly.

Objective:To investigate the clinical and chest computed tomography (CT) findings of children with drug-resistant pulmonary tuberculosis (DR-PTB) and drug-sensitive pulmonary tuberculosis (DS-PTB) in Sichuan region.Methods:Active pulmonary tuberculosis children with positive sputum Mycobacterium tuberculosis culture were collected and divided into the drug-resistant and drug-sensitive groups according to the results of the drug susceptibility test who admitted to the Public Health Clinical Center of Chengdu from January 2020 to October 2024. The general data such as age, ethnic, gender, initial or retreatment regimens, body mass index (BMI), clinical symptoms such as cough, sputum, fever, and chest CT features were compared between the two groups. Non-parametric rank sum test, chi-square test or Fisher′s exact probability method were used for comparison between groups. The CT risk factors for the development of DR-PTB were analyzed using binary logistic regression. Results:A total of 110 children were enrolled, including 44(40.00%) males and 66(60.00%) females, with the age of 13.00(12.00, 14.00) years (range from 5.00 to 14.00 years). There were 28 cases in drug-resistant group and 82 in drug-sensitive group. Comparison of the clinical data between the two groups showed that the percentages of retreated patients (25.00%(7/28)) and ethnic minorities (78.57%(22/28)) in the drug-resistant group were higher than those in the drug-sensitive group (1.22%(1/82) and 57.32%(47/82)), which were both statistically significant (Fisher′s exact probability method and χ2=4.03, respectively, both P<0.05). The age of the children in the drug-resistant group was 13.00 (12.00, 13.50) years, which was younger than the age of 13.00 (12.00, 14.00) years in the drug-sensitive group ( U=828.00, P=0.021). There were no statistically differences of gender, BMI, history of bacillus Calmette-Guérin (BCG) vaccination, history of exposure to tuberculosis patients, positive immunological diagnostic tests, and the incidences of cough, sputum, fever, wheezing, shortness of breath and chest pain between the two groups (all P>0.05). Chest CT signs showed that lung lesions involving both right and left lobes accounted for 53.57%(15/28) in the drug-resistant group, and the incidence of solid pulmonary parenchyma (including wall-less cavities) was 92.86%(26/28), which were both higher than those in the drug-sensitive group (15.85%(13/82) and 70.73%(58/82)), and the differences were statistically significant ( χ2=6.95 and 5.66, respectively, both P<0.05). The incidence of small nodules/tree-in-bud pattern in the drug-sensitive group was higher than that in the drug-resistant group (98.78%(81/82) vs 82.14%(23/28)), and the difference was statistically significant (Fisher′s exact probability method, P=0.004). Binary logistic regression showed that bilateral lung involvement and solid pulmonary parenchyma (including wall-less cavities) were positively correlated with DR-PTB in children (odds ratio ( OR)=4.487, 95% confidence internal ( CI) 1.561 to 12.897, P=0.005 and OR=9.670, 95% CI 1.626 to 57.529, P=0.013, respectively). The occurence of small nodules/tree-in-bud was negatively correlated with DR-PTB in children ( OR=0.058, 95% CI 0.006 to 0.584, P=0.016). Conclusions:Among pulmonary tuberculosis children in Sichuan region, ethnic minorities and retreatment patients with DR-PTB are more common than thoses with DS-PTB. Chest CT findings of DR-PTB mainly show multiple solid pulmonary parenchyma with wall-less cavities, and the lesions often involve right and left lung lobes, whereas DS-PTB CT findings mainly show small nodules/tree-in-bud predominantly.