OBJECTIVES: To analyze the differential expression and biological functions of circRNAs in the anterior lens capsules of high myopic patients with cataract and their pathogenic roles in the development of this condition. METHODS: Anterior lens capsule specimens were collected intraoperatively from 36 patients with age-related cataract (ARC) and 36 high myopic patients with cataract. Among these, 18 specimens from each group were selected for whole transcriptome sequencing and biological analysis, and the remaining 36 specimens were used for validation of circPDGFRA, circFOXJ3, hsa_circ_0004767, hsa_circ_0007528, ciCRIM1, circMAN1A2, circSLC5A3, and circPTK2 expressions using RT-qPCR. hsa_circ_0007528 was selected for cell experiments to examine its effects on proliferation, migration, and apoptosis of lens epithelial cells (LECs). RESULTS: A total of 16 192 circRNAs were detected in the specimens from both groups, among which 62 circRNAs were differentially expressed (29 upregulated and 33 downregulated). GO and KEGG analyses revealed that the differentially expressed circRNAs were primarily localized in the cytoplasm, nucleoplasm, and endoplasmic reticulum, and were involved in signaling pathways associated with Gap junction and the PI3K-Akt, NF-κB, Jak-STAT, HIF-1, and MAPK signaling pathways. The ceRNA network predicted multiple target genes. RT-qPCR validation results were consistent with the sequencing data. In the LECs, upregulation of hsa_circ_0007528 significantly inhibited cell proliferation and migration and obviously promoted cell apoptosis. CONCLUSIONS The expression profile of circRNAs in the anterior lens capsule of high myopic patients with cataract differs from that of ARC patients. Upregulation of hsa_circ_0007528 inhibits LEC proliferation and migration and promotes cell apoptosis.
Humans ; Cataract/complications* ; RNA, Circular ; Myopia/genetics* ; Apoptosis ; Cell Proliferation ; Epithelial Cells ; Cell Movement ; Anterior Capsule of the Lens/metabolism* ; Male ; Female

PURPOSE: To report a case of recurrent uveitis associated with autoimmune liver disease. CASE SUMMARY: A 50-year-old female with severe fatigue and arthritis visited the ophthalmology department due to decreased visual acuity and discomfort in her right eye for ten days. She had intermittent injection and blurred vision in both eyes for 30 years. Slit lamp examination of her right eye showed keratic precipitates, pigment deposits on the anterior capsule of the lens and anterior chamber cells; fundus examination was normal without any sign of chorioretinitis. Inflammatory reaction was improved after steroid and cycloplegic eye drop treatment. Two months later, her left eye developed anterior uveitis. Inflammation was well controlled with steroid and cycloplegic eye drop treatment. To evaluate the cause of uveitis and associated systemic disease, serological testing was performed, and abnormal elevation of liver enzymes was detected. The patient was referred to the Gastroenterology Department and diagnosed with autoimmune liver disease. Oral ursodeoxycholic acid was prescribed. Liver function profile improved to normal range, and the patient is currently under routine follow-up with no sign of recurrent uveitis. CONCLUSIONS: When a patient with recurrent uveitis presents symptoms such as nausea, fatigue, abdominal pain, jaundice or abnormal liver profile, association with autoimmune liver disease should be considered.
Abdominal Pain ; Anterior Capsule of the Lens ; Anterior Chamber ; Arthritis ; Chorioretinitis ; Fatigue ; Female ; Follow-Up Studies ; Gastroenterology ; Hepatitis, Autoimmune ; Humans ; Inflammation ; Jaundice ; Liver ; Liver Diseases* ; Middle Aged ; Nausea ; Ophthalmology ; Reference Values ; Serologic Tests ; Ursodeoxycholic Acid ; Uveitis* ; Uveitis, Anterior ; Visual Acuity

PURPOSE: In this study, we examined the stability of the lens-angle supporter (LAS) for accommodation restoration by comparing intraocular lens (IOL) location, after-cataract and ciliary body damage after cataract surgery in rabbits. METHODS: Eight rabbits were divided into experimental and control groups of four rabbits each. Phacoemulsification and irrigation and aspiration were performed in all rabbits. This was followed by an LAS and IOL insertion in the four experimental rabbits. In the four control rabbits, only an IOL insertion was performed. Six months after the surgery, the location of the IOL, the conditions of the lens capsule and ciliary body were evaluated using a slitl-amp examination and Miyake-Apple view. RESULTS: For the experimental group, the ultrasound biomicroscope results showed normal LAS and IOL positioning in all four cases. According to the slitlamp examination and Miyake-Apple view, the IOL was positioned at the center, with less after-cataract and damage to the ciliary body. For the control group, ultrasound biomicroscope results indicated a higher IOL position than normal, as well as a single case of IOL decentering. According to the slit-lamp examination and Miyake-Apple view, the IOL was decentered with more severe after-cataract and ciliary body damage. CONCLUSIONS: The LAS has the potential to maintain a stable IOL position while producing less after-cataract when used in lens-angle reconstruction for correction of presbyopia. Moreover, LAS implantation incurs less damage to the ciliary body.
Accommodation, Ocular/physiology ; Animals ; Anterior Eye Segment ; Ciliary Body/injuries ; Disease Models, Animal ; Eye Injuries/*surgery ; Lens Capsule, Crystalline/*surgery ; *Lens Implantation, Intraocular ; Microscopy, Acoustic ; *Phacoemulsification ; Rabbits ; *Reconstructive Surgical Procedures

PURPOSE: To report a case of rotational flap of Tenon's capsule for hypotony with choroidal detachment occurring after trabeculectomy and needle revision (NR) of a nonfunctioning bleb. METHODS: An 15-year-old female, suffering acute angle-closure glaucoma with lens dislocation into anterior chamber, underwent pars plana vitrectomy, lensectomy, and intraocular lens scleral fixation. Three months later, trabeculectomy with mitomycin C (MMC) and NR with MMC were performed to decrease intraocular pressure (IOP). After 5th NR, hypotony with choroidal detachment occurred. Consequently, bleb revision and rotation flap of the autologous Tenon's capsule were performed. RESULTS: One week after surgery, IOP was 18 mmHg, and a diffuse bleb was formed. Choroidal detachment improved with remaining vascular tortuisity and chorioretinal folds. At five months, best corrected visual acuity was 0.5, IOP was 20 mmHg, vascular tortuisity decreased, and chorioretinal folds disappeared in the macula. CONCLUSIONS: Hypotony with choroidal detachment occurring after needle revision was treated effectively with bleb revision and rotation at flap of autologous Tenon's capsule.
Adolescent ; Anterior Chamber ; Blister ; Choroid ; Female ; Glaucoma, Angle-Closure ; Humans ; Intraocular Pressure ; Lens Subluxation ; Lenses, Intraocular ; Mitomycin ; Needles ; Tenon Capsule* ; Trabeculectomy ; Visual Acuity ; Vitrectomy

Animal ; FOOD COLORING AGENTS/ADMINISTRATION & DOSAGE ; FOOD COLORING AGENTS/ADVERSE EFFECTS ; FOOD COLORING AGENTS/DIAGNOSTIC USE ; ANTERIOR CAPSULE OF THE LENS ; ANIMAL ; RABBITS

PURPOSE: We assessed the effect of transplantation of capsule on the change of gross and fine structures of the experimentally induced capsular rupture of porcine lens. METHODS: A rupture of 1.0, 3.0 and 5.0 mm in diameter was made on the anterior capsule of the lens and the anterior capsule was transplanted using lyophilized fibrin adhesive(Greenplast(R)) to cover the rupture. RESULTS: Gross and transmission electron microscopic examinations revealed at day 1, 3 and 7 that the progression of lens opacification was delayed and the stability of microscopic fine structure was less disturbed in the lenses with a rupture of 3.0 mm or less or receiving capsule transplantation less than 4 days after capsule rupture. In the lenses with a rupture of 5 mm or larger or receiving transplantation more than 3 days after capsule rupture, there were no significant differences in gross and microscopic findings between transplantation and control groups CONCLUSION: We applied transplantation concept on the lens. Further studies are needed for transplantation of lens capsule.
Anterior Capsule of the Lens ; Fibrin ; Rupture*

When the continuous circular capsulorhexis (CCC) is being performed, the nucleus delivery using nucleus dislocation into the anterior chamber is safer and easier than using conventional "push and pull" method to maintain an intact lens capsule. This method include such procedures that after CCC, the nucleus being freed in the capsular bag by hydrodissection and hydrodelineation, then hooked with a Sinskey hook and drawn out to the anterior chamber by rotation. It may damage the corneal endothelium because of the manipulation in the anterior chamber. To investigate an effect of this method on the corneal endothelium, we performed two months time analysis of changes of the central corneal endothelial cell density (CECD) in two groups--a group with an extracapsular cataract extraction (ECCE) using nucleus dislocation into the anterior chamber and a group with a conventional ECCE. Eighteen eyes of 18 cataract patients who were operated on with ECCE using nucleus dislocation into the anterior chamber method, and ten eyes of 10 cataract patients who were operated on with a conventional ECCE method were included. The CECD was measured by specular microscopy, preoperatively, postoperatively at 1 month and 2 months. The average endothelial cell loss at postoperative 1 month was 7.20 +/- 2.98% in the experimental group and 7.88 +/- 2.93% in the control group, and at postoperative 2 months was 9.05 +/- 2.96% in the experimental group and 9.34 +/- 2.95% in the control group. The change in CECD between two groups was not statistically significant.
Adult ; Aged ; Anterior Chamber/*surgery ; Cataract Extraction/*methods ; Cell Count ; Endothelium, Corneal/*pathology ; Female ; Humans ; Lens Capsule, Crystalline/*surgery ; Lens Nucleus, Crystalline/*surgery ; Male ; Middle Aged

We anlized CT of 29 cases of various orbital disease in pediatric age group. Diagnoses were confirmed byeither operation or direct ophthalmoscopy. The patients were examined during the period of last 3 years. Theresults were as follows: 1. Of 29 cases, 21 were male and 8 were female, and the age ranged from 20 days to 15years. 2. Intraorbital tumors were 13 cases consisting of 7 retinoblastoma, 3 pseudotumor, and 1 each ofcongenital teratoma, cavernous lymphanngioma, and optic glioma, Of remaining 16 cases, 6 had orbital fracture, 5persistent hyperplastic primary viterous(PHPV), 3 vitreous opacity, and 2 primary glaucoma. 3. The CT findings ofthe retinoblastoma were a lobulated or oval soft tissue mass denisty (40-60HU) extending into the vitreous wtihoutsignificant enhancement on postcontrast scan. Of 7 cases, 4 had calcifications within the tumors. The extraglobalextension of the tumor was shown in 2 patients demonstrating markedly thickened sclera with enhancement anddilated optic nerve. 4. There were 5 patients with PHPV having a history of white pupil since birth, and all wereaffected unilateraly. Four patients had vitreous opacity and 3 of those showed either linear or branchingtree-like densities within the vitreous on the postcontrast scan. Another one had a lobulated increased densityprotruding into the vitreous which was difficult to differenciate from the similar finding of retinoblastoma.Remaining one case demonstrated a deformed and decreased density in the lesional lens without chaneg in thedensity of the vitreous. This was confirmed to be caused by persistence of the embryonic hyaloid artery attachedto the posterior capsule of the lens. 5. There were 3 patients with vitreous opacity and 2 of them were due toretinal detachment and 1 was caused by retrolental fibroplasia. The CT findings of retinal detachement werehomogenous or heterogenous opacities in the vitreous. In a case of retrolental fibroplasia, irregular, band-likedensity was seen along the posterior wall of the globe bilaterally. 6. Two cases of primary glaucoma showed largeglobe with a widened anterior chamber. 7. Among 6 cases of orbital fractures, 5 were blow-out fracture. Theblow-out fractures showed fractures of the medial orbital wall or orbital floor with herniated intraorbitalcontents into the neighboring ethmoid or maxillary sinus.
Anterior Chamber ; Arteries ; Diagnosis ; Female ; Glaucoma ; Humans ; Male ; Maxillary Sinus ; Ophthalmoscopy ; Optic Nerve ; Optic Nerve Glioma ; Orbit ; Orbital Diseases ; Orbital Fractures ; Parturition ; Posterior Capsule of the Lens ; Pupil ; Retinaldehyde ; Retinoblastoma ; Retinopathy of Prematurity ; Sclera ; Teratoma