To investigate the function and regulation mechanism of ATP-binding cassette, subfamily G, member 2 (ABCG2) in retinoblastoma cancer stem cells (RCSCs), a long-term culture of RCSCs from WERI-Rb1 cell line was successfully established based on the high expression level of ABCG2 on the surface of RCSCs. To further explore the molecular mechanism of ABCG2 on RCSCs, a microRNA that specifically targets ABCG2 was predicted. Subsequently, miR-3163 was selected and confirmed as the ABCG2-regulating microRNA. Overexpression of miR-3163 led to a significant decrease in ABCG2 expression. Additionally, ABCG2 loss-of-function induced anti-proliferation and apoptosis-promoting functions in RCSCs, and multidrug resistance to cisplatin, carboplatin, vincristine, doxorubicin, and etoposide was greatly improved in these cells. Our data suggest that miR-3163 has a significant impact on ABCG2 expression and can influence proliferation, apoptosis, and drug resistance in RCSCs. This work may provide new therapeutic targets for retinoblastoma.
3' Untranslated Regions ; ATP Binding Cassette Transporter, Sub-Family G, Member 2/antagonists & inhibitors/genetics/*metabolism ; Antagomirs/metabolism ; Antineoplastic Agents/toxicity ; Apoptosis/drug effects ; Base Sequence ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Drug Resistance, Neoplasm/drug effects ; Gene Silencing ; Humans ; MicroRNAs/antagonists & inhibitors/genetics/*metabolism ; Neoplasm Proteins/antagonists & inhibitors/genetics/*metabolism ; Neoplastic Stem Cells/*metabolism ; Retinoblastoma/metabolism/pathology ; Sequence Alignment ; Transfection

3' Untranslated Regions ; Animals ; Antagomirs ; metabolism ; Base Sequence ; Binding Sites ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Humans ; Mice ; MicroRNAs ; antagonists & inhibitors ; genetics ; metabolism ; RNA Interference ; RNA, Messenger ; metabolism ; RNA, Small Interfering ; metabolism ; Rats ; Sequence Alignment ; Snail Family Transcription Factors ; antagonists & inhibitors ; genetics ; metabolism ; Stomach Neoplasms ; genetics ; pathology