Objective:To explore the effects three of anesthetics(tribromoethanol,isoflurane,and pentobarbital so-dium)on the outcome of mice with post-stroke dysphagia(PSD)induced by photothrombosis(PT)method,and to e-valuate which anesthetic is more suitable for the preparation of this model.Methods:Sixty-six male C57BL/6J mice were divided into Tribromoethanol group,Isoflurane group,Pentobarbital sodium group and Sham group.The post-stroke dysphagia model was established by PT.Before and 5 min after modeling,a laser speckle imager was used to measure the regional cerebral blood flow(rCBF)decrease rate of mice and record the wake-up time of mice.Forty-eight hours after modeling,the mortality rate of PSD mice in three groups was recorded and the rCBF decrease rate was meas-ured again.The neurological function of mice was evaluated using the neurological deficit score,the water intake of mice was recorded using the 4-min drinking test,the infarct volume ratio was measured using the TTC staining method,and the swallowing counts induced by water administration was recorded using the multichannel physiological recorder MP160 and the myoelectric area of the swallowing muscle was calculated.Results:There was no statistical difference in the percentage of decrease in rCBF,infarct volume ratio,neurological deficit score,water intake,swallowing counts,and myoelectric area of swallowing muscle among the three groups of PSD mice 48 h after modeling(P＞0.05).Com-pared with the Tribromoethanol group and the Pentobarbital sodium group,the rCBF of the mice in the Isoflurane group decreased rapidly within 5 min(P＜0.05),and the mortality rate of the mice was lower and the awakening time was shorter.(P＜0.05).Conclusion:The use of different anesthesia will affect the mortality rate,wake-up time and the downward trend of rCBF within 48 h after modeling of PSD mice.Among the three anesthetics,isoflurane is more suit-able as an anesthetic for the PSD mouse model.

[Objective]To explore the therapeutic approaches for meridian-tendon diseases documented in medical manuscripts of the Qin and Han Dynasties,and provide insights for modern treatment of meridian-tendon diseases.[Methods]By compiling and analyzing the meridian-tendon diseases related information extracted from medical bamboo slips and silk such as Mawangdui Medical Texts,Zhangjiashan Han Medical Bamboo,Tianhui Medical Bamboo Slips and Wuwei Han Medical Slips,which investigate the understanding of Qin-Han period physicians,regarding of the etiology,pathogenesis,symptoms and treatment methods of meridian-tendon diseases,with a focus on extracting records related to their treatment methods,systematically summarizing specific therapeutic approaches and comprehensively outlining the treatment system for meridian-tendon diseases in Qin-Han medical texts.[Results]The study revealed that Qin-Han period physicians primarily attributed the etiology and pathogenesis of meridian-tendon diseases to the invasion of wind,cold,and damp pathogens,whose clinical manifestations included limb pain,numbness,reduced function and motor impairment.Therapeutically,a comprehensive system integrating internal and external treatments was established:external treatments encompassed repositioning,moxibustion,ironing,Bian stone therapy,acupuncture and massage therapy;internal treatments included single-drug prescriptions and traditional Chinese medicine(TCM)compounds;preventive healthcare measures comprised Daoyin,dietary therapy,health supplements,cupping therapy and Zhuyoushu.These approaches collectively formed a holistic treatment and prevention system for meridian-tendon diseases in Qin-Han medical texts.[Conclusion]The treatment of meridian-tendon diseases during this period exhibited characteristics of combining internal and external therapies,using medications and diet concurrently,applying a single technique for multiple purposes,emphasizing both prevention and treatment,and widely utilizing alcohol.

Objective:To explore the effects three of anesthetics(tribromoethanol,isoflurane,and pentobarbital so-dium)on the outcome of mice with post-stroke dysphagia(PSD)induced by photothrombosis(PT)method,and to e-valuate which anesthetic is more suitable for the preparation of this model.Methods:Sixty-six male C57BL/6J mice were divided into Tribromoethanol group,Isoflurane group,Pentobarbital sodium group and Sham group.The post-stroke dysphagia model was established by PT.Before and 5 min after modeling,a laser speckle imager was used to measure the regional cerebral blood flow(rCBF)decrease rate of mice and record the wake-up time of mice.Forty-eight hours after modeling,the mortality rate of PSD mice in three groups was recorded and the rCBF decrease rate was meas-ured again.The neurological function of mice was evaluated using the neurological deficit score,the water intake of mice was recorded using the 4-min drinking test,the infarct volume ratio was measured using the TTC staining method,and the swallowing counts induced by water administration was recorded using the multichannel physiological recorder MP160 and the myoelectric area of the swallowing muscle was calculated.Results:There was no statistical difference in the percentage of decrease in rCBF,infarct volume ratio,neurological deficit score,water intake,swallowing counts,and myoelectric area of swallowing muscle among the three groups of PSD mice 48 h after modeling(P＞0.05).Com-pared with the Tribromoethanol group and the Pentobarbital sodium group,the rCBF of the mice in the Isoflurane group decreased rapidly within 5 min(P＜0.05),and the mortality rate of the mice was lower and the awakening time was shorter.(P＜0.05).Conclusion:The use of different anesthesia will affect the mortality rate,wake-up time and the downward trend of rCBF within 48 h after modeling of PSD mice.Among the three anesthetics,isoflurane is more suit-able as an anesthetic for the PSD mouse model.

[Objective]To explore the therapeutic approaches for meridian-tendon diseases documented in medical manuscripts of the Qin and Han Dynasties,and provide insights for modern treatment of meridian-tendon diseases.[Methods]By compiling and analyzing the meridian-tendon diseases related information extracted from medical bamboo slips and silk such as Mawangdui Medical Texts,Zhangjiashan Han Medical Bamboo,Tianhui Medical Bamboo Slips and Wuwei Han Medical Slips,which investigate the understanding of Qin-Han period physicians,regarding of the etiology,pathogenesis,symptoms and treatment methods of meridian-tendon diseases,with a focus on extracting records related to their treatment methods,systematically summarizing specific therapeutic approaches and comprehensively outlining the treatment system for meridian-tendon diseases in Qin-Han medical texts.[Results]The study revealed that Qin-Han period physicians primarily attributed the etiology and pathogenesis of meridian-tendon diseases to the invasion of wind,cold,and damp pathogens,whose clinical manifestations included limb pain,numbness,reduced function and motor impairment.Therapeutically,a comprehensive system integrating internal and external treatments was established:external treatments encompassed repositioning,moxibustion,ironing,Bian stone therapy,acupuncture and massage therapy;internal treatments included single-drug prescriptions and traditional Chinese medicine(TCM)compounds;preventive healthcare measures comprised Daoyin,dietary therapy,health supplements,cupping therapy and Zhuyoushu.These approaches collectively formed a holistic treatment and prevention system for meridian-tendon diseases in Qin-Han medical texts.[Conclusion]The treatment of meridian-tendon diseases during this period exhibited characteristics of combining internal and external therapies,using medications and diet concurrently,applying a single technique for multiple purposes,emphasizing both prevention and treatment,and widely utilizing alcohol.

Objective:To establish a feasible mouse model of post-stroke dysphagia(PSD).Methods:Thirty C57BL/6 male mice were randomly divided into a sham-operated group(Sham)and a model group(PSD),and the PSD mouse model was made by the photothrombosis method(PT)method,and the sham-operated group was only injec-ted with rose bengal staining solution in the tail vein.The cerebral blood flow of the mice was measured by laser scatter imaging,the ratio of cerebral infarct area was detected by TTC staining,the electromyographic area of the in vivo pha-ryngeal muscle group of mice swallowing was recorded by a multi-conductor physiological recorder MP160,the drinking function of the mice was measured by the 4-min water drinking experiment,and the weight changes were recorded,respectively,at 1,3,and 7 d.Results:Cerebral blood flow decreased at all time points,with a sharp drop in cerebral blood flow at 1 d,gradual recovery of cerebral blood flowat 3 and 7 d,establishment of collateral circulation,and gradu-al reduction of cerebral infarction area;compared with the Sham group,the myoelectric area of the PSD group was reduced at 1 and 3 d(P＜0.05),but with a trend of gradual recovery,and there was no significant difference between the PSD group and the Sham group at 7 d,and water consumption and weight decreased at 4 min at 1,3,and 7 d(P＜0.05).Conclusion:The mice showed some degree of dysphagia symptoms and are expected to be a translational model for PSD.

This study aimed at exploring the features of the body constitution and syndrome types of TCM in patients with pSS.General information of 60 patients with pSS was collected.Their TCM syndrome types and TCM body constitution types were examed,while the distribution of pSS syndrome types in terms of their body constitution was analyzed.Among 60 pSS patients,58 of them (96.67％) were female with the average age of 52 years and an average course of 63 months.Their usual residence was Xinjiang.Their most common first symptoms included thirst,dry eyes and joint pain,and the damage of skin and mucous membranes and blood system was rare.It was found that the syndrome of both qi and yin deficiency was the most common (48.33％),followed by the impregnation syndrome of heat and dampness (26.67％) and the syndrome of the weakness of spleen qi (25％) in pSS patients.Yin deficiency (58.33％) and yang deficiency (58.33％) in the body constitution of TCM accounted for decent proportions,followed by the constitutions of qi stagnation (48.33％),qi deficiency (45.00％),blood stasis (38.33％),phlegm-dampness (36.67％),damp-heat (18.33％),allergic (13.33％) and yin-yang harmony (1.67％).Those without the health education of pSS accounted for 51.11％ in cases of palindromia.In conclusion,yin deficiency and yang deficiency constitutions were most common,at most,the body constitution of both qi and yin deficiency,in pSS patients in accordance with TCM body constitution.Most pSS patients of palindromia didn't received professional health education of pSS.All the causes above suggested that we should make individualized treatment programs according to clinical syndrome types and the characteristics of TCM body constitution in pSS patients for strengthening the health management of pSS and improving their quality of life.

This study was aimed to evaluate the clinical effectiveness of Ban-Xia Hou-Po (BXHP) decoction in the treatment of globus hystericus. Related literatures were systemically searched in the China National Knowledge Infrastructure (CNKI, 1979-2013), Weipu Database for Chinese Technical Periodicals (1989-2013), and PubMed/Medline. The methodological quality of included trials was assessed based on the Jadad scale. The available data was analyzed with RevMan software (version 5.2.0) provided by the Cochrane collaboration. The results showed that a total of 6 randomized controlled trials (RCTs), which contained 649 globus hystericus cases, were included. The meta-analysis showed that compared with modern medicine, the total therapeutic effect of BXHP decoction was RR=1.15, 95%CI (1.08, 1.23); compared with modern medicine treatment alone, the total therapeutic effect of combination between BXHP decoction and modern medicine was RR=1.43, 95%CI (1.19, 1.73). It was concluded that the current clinical evidences showed that the effectiveness of BXHP decoction alone or combined with modern medicine in the treatment of globus hystericus was higher than the treatment only with modern medicine. And there was no obvious side effect. However, due to the low-quality studies and small amount of samples, the results still required to be confirmed with more high-quality, multi-center, and large sample RCTs.