Animal experiments are crucial in evaluating the preclinical safety and efficacy of new dental materials, drugs, instruments, and equipment by identifying and eliminating potential health risks to humans. An international team of several dental experts formulated a guideline named Preferred Reporting Items for Animal Studiesin Endodontology (PRIASE) 2021. Consisting of 11 domains, 43 individual items, and a flowchart. PRIASE provides guidance for animal experiments in dentistry and improves the quality of experiment design and reporting. This work introduces the process and basic content of the guideline and interprets the key items of its checklist with specific examples to provide reference for the reporting of animal experiment in dentistry in China.
Animals ; Animal Experimentation/standards* ; Checklist ; China ; Endodontics ; Guidelines as Topic ; Research Design

This study aims to assess the methodological and reporting quality of animal experimental studies on the treatment of cervical radiculopathy(CR) with traditional Chinese medicine(TCM), analyze the deficiencies during the experimental process, and develop the methods to enhance the quality of such studies. The related articles were retrieved from CNKI, Wanfang, VIP, SinoMed, PubMed, EMbase, Cochrane Library, and Web of Science. The methodological quality and reporting quality of the included studies were evaluated according to the risk of bias tool of the Systematic Review Centre for Laboratory Animal Experimentation(SYRCLE) and the Animal Research: Reporting of in vivo Experiments(ARRIVE) 2.0 guidelines, respectively. A total of 4 086 articles were initially screened, in which 71 articles met the inclusion criteria. The SYRCLE's risk of bias tool revealed selection bias, performance bias, detection bias, and attrition bias of the included studies. The aspects for improvement were identified in the randomization of animal grouping, experimental implementation and outcome assessment, blinding, reporting baseline characteristics, and handling incomplete data. The essential item assessment of the ARRIVE 2.0 guidelines showed high risks in sample size determination, inclusion and exclusion criteria, randomization, blinding, outcome assessment, statistical methods, experimental procedures, and results reporting. Additionally, there were high risks in items recommended by ARRIVE 2.0 guidelines, including study background, ethical statements, animal care, interpretation/scientific implications, generalizability/translation, experimental protocol registration, data availability, and conflict of interest declaration. The existing animal experimental studies about the TCM treatment of CR exhibited methodological and reporting deficiencies. We recommend that researchers refer to the SYRCLE's risk of bias tool and the ARRIVE 2.0 guidelines to rigorously design, implement, and report experiments in a standardized manner, thereby enhancing the scientific, authentic, and reproducible properties of the experiments.
Radiculopathy/therapy* ; Animals ; Medicine, Chinese Traditional ; Drugs, Chinese Herbal/administration & dosage* ; Humans ; Animal Experimentation/standards* ; Disease Models, Animal ; Research Design/standards*

OBJECTIVETo investigate the current situation of animal experiments by analysis and evaluation of relevant articles published in the Chinese Journal of Burns during 2000 to 2004.

METHODSAll articles concerning the results of animal experiments related to the treatment and medical studies in the past 5 years were analysed according to the international standard , especially in serval aspects, such as sample size, randomization method, sample choice, comparison measure, baseline comparability, estimate index, statistics method, and so oN.

RESULTSDuring 2000 to 2004, totally 1 116 papers were published in the journal, among them 81 papers were (accounted for 7.3% ) of all articles were reports of treatment related experiments. Out of 81 papers, in 69 experiments ( accounted for 85. 2% ) there was randomized control group, and the main problems lay in calculation of the number of samples, the application of randomization method, and analysis of statistics.

CONCLUSIONApplication of the randomized control modality in animal experiment is popular in our country, but the design of some studies is not so rigorous. Therefore, we should improve the design of every research project before the experiment is carried out.

Animal Experimentation ; standards ; Animals ; Burns ; Evaluation Studies as Topic ; Periodicals as Topic ; statistics & numerical data