Objective To establish a method for concentration determination of menthol in the nasal in -situ gel spray. Methods Gas chromatography (GC) was performed on the column of Agilent DB-WAX capillary column (30m ×0.25 mm ×0.25μm), with the column temperature of 120 ℃.With nitrogen gas used as carrier gas , hydrogen flame ion detector (FID) was used, the detection temperature was 260℃, injection temperature was 220℃, injection volume was 1μl and column flow rate was 1.0 ml/min. Results For menthol, there was good linear relationship within the range of mass concentration at 0.02~0.20 mg/mL (r=0.9991). Precision, reproducibility and stability test were all lower than 2%, the sample recovery rate was 95.1%~103.4%and relative stand-ard deviation (RSD) was 2.3%(n=6).Conclusion The established method had the following advantages:easy operation, high re-liability and good reproducibility , and it could be used for the content determination of menthol in the nasal in -situ gel spray .