Background: Bioactive compounds derived from plants have been extensively studied and widely applied in cosmetic and dermatological fields. In particular, plant-derived polyphenols and anthraquinones are known for their antioxidant properties as well as their ability to inhibit elastase enzyme activity. Elastase is a key proteolytic enzyme responsible for degrading elastin, a structural protein essential for maintaining skin elasticity. Excessive elastase activity contributes to skin aging and wrinkle formation. Therefore, the identification of plant-derived compounds capable of interacting with elastase is of significant importance for improving the composition and efficacy of cosmetic products. Aim: To identify bioactive compounds in the extract of Rheum undulatum L. and to investigate their interaction with elastase enzyme activity. Materials and Methods: The root of Rheum undulatum L. was used as the study material. Sample preparation was performed by grinding the plant root followed by extraction using 50% ethanol assisted by an ultrasonic water bath. The obtained extract was evaporated to dryness using a vacuum centrifugation system to remove the solvent completely. A total of 10 mg of the dried extract was dissolved in 100 µL methanol and 900 µL water, then filtered through a 0.1 µm membrane filter. Subsequently, two sample sets were prepared: a control sample and an elastase-incubated sample at a 1:1 ratio. Both were analyzed using liquid chromatography–mass spectrometry (LC-MS). Data processing was performed using Agilent MassHunter Qualitative Analysis software (version 10.0). Results: LC-MS analysis revealed a total of 16 bioactive compounds in the root extract of Rheum undulatum L. Comparative analysis between the control and elastase-treated samples demonstrated a decrease in the peak areas of gluconic acid and citric acid, while an increase in the peak area of emodin was observed. These changes indicate compositional differences between the two sample conditions. Conclusion The LC-MS analysis of Rheum undulatum L. root extract identified multiple bioactive compounds belonging to anthraquinone, phenolic, and flavonoid classes. Variations in peak intensities between control and elastase-treated samples suggest potential interactions between certain compounds and the enzyme. These findings indicate that the root extract contains biologically active constituents capable of modulating elastase activity, supporting its potential application in anti-aging and cosmetic formulations.

Background: Histopathological examination of skin tissue plays an important role in the diagnosis of dermatological diseases; however, data on surgically excised skin specimens remain limited in Mongolia. Aim: To classify benign and malignant tumors based on histopathological findings of surgically excised skin tissue specimens, determine surgical margin status, and evaluate the age and sex distribution of these conditions. Materials and Methods: This retrospective observational study was conducted using data on surgically excised tissue specimens collected in the outpatient department of the National Dermatology Center between January 2023 and March 2026. A total of 361 patients were identified. Four cases with indeterminate histopathological diagnoses were excluded, leaving 357 patients for final analysis. Data on age, sex, diagnosis, and surgical margin status were collected and analyzed on Microsoft Excel programm. Histopathological diagnoses were classified as benign or malignant tumors. Associations between categorical variables were assessed using the chi-square test, with statistical significance set at p < 0.05. Results: Among 357 patients, 135 (37.8%) were male and 222 (62.2%) were female, with a mean age of 41.8 years. The 20–39-year age group accounted for the largest proportion of cases (39.5%). Histopathological examination showed that 326 cases (91.32%) were benign and 31 cases (8.68%) were malignant. Among benign tumors, melanocytic nevus was the most common diagnosis, while basal cell carcinoma was the most common malignant tumor. The frequency of malignant tumors increased significantly with age (p < 0.001). Patients aged ≥60 years had significantly higher odds of malignancy compared with younger patients (OR = 8.19, 95% CI: 3.79–17.69). Malignant lesions were significantly more likely to have positive (involved) surgical margins than benign lesions. Conclusion Most surgically excised skin tissue specimens were benign; however, the likelihood of malignancy increased significantly with age. Surgical margin assessment is clinically important for improving prognosis, reducing the risk of recurrence, and guiding treatment management.

Background: Rosai–Dorfman disease (RDD) is a rare, benign disorder of non-Langerhans cell histiocytic origin with an unknown etiology, which can affect the skin and other organ systems. The incidence in the United States is approximately 1:200,000. In Mongolia, four cases have been registered over the past five years under the ICD-10 code D76.0. Case presentation: A 59-year-old female patient initially presented to the Dermatology Department of the National Center for Dermatology with clustered, elevated lesions on the medial aspect of the right thigh, including papules, plaques, and nodules of round to oval shape. Additionally, multiple erythematous macules and papules were observed on both lower legs. For diagnostic purposes, a histopathological examination was performed, revealing histiocytes with abundant cytoplasm and lymphocytes within the cytoplasm (emperipolesis). The diagnosis was confirmed based on dermoscopic and immunohistochemical findings. Management: The patient was referred to the Mongolian-Japanese Hospital of MNUMS, where the remaining nodular lesions were surgically excised in the surgical department. Further follow-up was referred to a hematologist. Computed tomography (CT) imaging was recommended. Cutaneous-limited RDD may resolve spontaneously in some cases; however, when multiple organ systems are involved or when associated forms are present, referral to hematology and oncology specialists for systemic therapy is required. Conclusion It is important to differentiate this disease from other dermatological conditions presented with macules, papules, and nodules through clinical evaluation, histopathology, immunohistochemistry, and dermoscopic examination.

Background: Saponins are secondary metabolites in plants that play an important role in defense mechanisms and physiological processes. Since stomatal cells are crucial for gas exchange and water balance in plant tissues, studying the anatomical and biochemical features of stomatal cells in saponin-rich plant species provides insight into the interactions between these compounds and cellular mechanisms. This serves as the basis of our study. Aim: To determine the structure, types, and functions of stomatal cells in saponin-containing medicinal plants Materials and Methods: During June–September 2024, nine species of saponin-containing medicinal plants were collected from Bulgan, Tuv provinces, and “Gorkhi-Terelj National Park” in Mongolia. Prepared microscopic specimens were examined using macroscopic and microscopic techniques to study the structure, position, number, and epidermal features of stomatal cells. Results: The study revealed that Anemone crinita Juz. exhibited the highest stomatal density (107 stomata/mm²) and epidermal cell density (229 cells/mm²), indicating strong adaptation and protective capacity in arid conditions. Vicia baicalensis (Turcz.) B. Fedtsch. showed the highest stomatal index (39.6), highlighting its significant role in regulating transpiration. Stomatal types varied among species: • Anomocytic stomata were observed in Potentilla multifida and Vicia baicalensis. • Anisocytic stomata were found in Delphinium grandiflorum and Ranunculus borealis. • Paracytic stomata were present in Gentiana algida, Adenophora remotiflora, Helianthemum nummularium, Anemone crinita, and Ranunculus acris. Conclution In the study of the structure, form, and number of stomatal cells in saponin-containing plants growing in Mongolia, Anemone crinita Juz. was found to have the highest number of stomatal and epidermal cells, indicating its high efficiency in gas exchange, water regulation, and protection against external stress. Furthermore, Vicia baicalensis (Turcz.) B.Fedtsch. showed the highest stomatal index, confirming its strong capacity for active regulation of gas exchange. The variation in stomatal cell types among plant species was identified as playing an important role in ecological and biological adaptation as well as protective mechanisms.

Background: Plants of the family Gentianaceae in Mongolia are rich in bitter iridoid glycosides, flavonoids, and polyphenolic compounds, and have been traditionally used in Mongolian medicine to promote digestion, protect the liver, and reduce fever. However, standardized information on the micro-morphology and histochemical characteristics of the widely distributed species Gentiana decumbens L.f. is scarce, limiting the assessment of its quality as a medicinal raw material. Aim: To identify the morphological and anatomical characteristics of the aerial and underground parts of Gentiana decumbens and to localize the distribution of starch, polysaccharides, and phenolic compounds using histochemical methods. Materials and Methods: Plant materials were collected in July 2024 from Tsagaan-Uul soum, Khuvsgul province, andshade-dried. Samples were softened in a water–ethanol–glycerol solution (1:1:1) for 24 hours, and transverse and longitudinal sections of stem, leaf, root, and floral parts (anther, petal, receptacle/bract) were prepared using a hand microtome. Sections were cleared with 10% chloral hydrate. Histochemical reagents included potassium dichromate (K2Cr2O7), ferric chloride (FeCl3), Lugol’s iodine (I₂+KI), thymol + concentrated H₂SO₄, and methylene blue. Slides were mounted in glycerin and examined under an Olympus light microscope at 40× and 100× magnifications, and images were recorded using a 12 MP digital camera. Results: Leaf: The leaf exhibits a dorsiventral structure with a dense palisade parenchyma on the adaxial side and a loosely arranged spongy mesophyll on the abaxial side. Stomata are hypostomatic, and collenchyma is well developed around the vascular bundles. Histochemical reactions were negative for starch and phenolic compounds. Stem: The stem consists of an epidermis, a wide cortex, a continuous ring of sclerenchyma, 12–15 collateral vascular bundles, and a broad central parenchyma. Histochemical tests showed a brownish-yellow coloration with potassium dichromate (K2Cr2O7), indicating the presence of polyphenols and lignin, and a brown coloration with Lugol’s iodine, confirming the presence of starch. Reactions with ferric chloride (FeCl₃), methylene blue, and thymol + H2SO4 were mostly negative in cross sections. However, in longitudinal sections, Lugol’s and thymol + H2SO4 showed positive reactions, suggesting the presence of starch and polysaccharides, respectively. Root: The root displays a primary structure with radial xylem and phloem, a distinct endodermis with Casparian strips, and a pericycle layer. Positive reactions were observed with K2Cr2O7 and FeCl₃, indicating phenolic compounds and lignin. Lugol’s iodine showed abundant starch granules in parenchyma cells. Receptacle / Bract: The receptacle is covered by a thick cuticular epidermis with trichomes, and composed of outer and central parenchyma layers containing numerous small closed (amphivasal-type) vascular bundles. Positive reactions were detected with K2Cr2O7 and FeCl₃, indicating polyphenols, flavonoids, and tannins; Lugol’s iodine confirmed the presence of starch; thymol + H2SO4 showed partial positivity for polysaccharides. Stamen (longitudinal section): The stamen structure includes epidermis, endothecium, pollen sacs, and a distinct vascular bundle. Positive staining was observed with K2Cr2O7, Lugol’s iodine, and FeCl3, confirming the presence of polyphenolic compounds, lignified elements, and starch. Conclusion The anatomical features of G. decumbens indicate adaptations to dry, high-light environments through well-developed mechanical strengthening (sclerenchymatous ring), efficient photosynthetic structure (compact palisade mesophyll), and nutrient storage (starch-rich pith and cortex). Histochemical analysis revealed the localization of polyphenolic compounds and starch/polysaccharides mainly in the root, stem, and floral organs, while absent in the leaves, demonstrating organ-specific accumulation patterns. These findings scientifically support the plant’s traditional medicinal uses for digestive stimulation and hepatoprotection.

Background: Accreditation of healthcare institutions serves as a fundamental mechanism for ensuring patient safety and validating the quality of medical services provided to the population. At Intermed Hospital, a quality measurement system for healthcare services has been established since 2015, encompassing 126 quality indicators at both institutional and departmental levels. This system facilitates continuous quality improvement efforts. In this context, quality indicators specific to the endoscopy department play a pivotal role in objectively assessing the quality of endoscopic services. Aim: To assess the quality indicators in gastrointestinal endoscopy unit. Materials and Methods: A retrospective single-center study was conducted by collecting data from the Intermed hospital’s electronic information systems which included HIS and PACS and Quality and Safety Department’s Database and the results were processed using the SPSS software. Ethical approval was granted by the Intermed hospital’s Scientific research committee. The quality of endoscopic services in the Intermed hospital was assessed based on: a) the average values of four quality indicators measured monthly; b) sample survey data from five categories of quality indicators. Results : Between 2016 and 2024, the quality indicators of the endoscopy unit measured as the level of early warning score evaluations for patients was 95.97%±3.33, the level of cases where peripheral blood oxygen saturation decreased during sedation was 1.54%±3.78, the level of cases where patients experienced paradoxiical response during sedation was 5.82%±1.75, surveillance culturing level for validation of endoscopy reprocessing was 11.6%. The endoscopic documentation quality by peer review showed 95.7-100%, the colonoscopy quality indicators were followings as adenoma detection rate: 24.5% Cecal intubation rate: 99.1%, 95.2%, Colonoscope withdrawal average time: 13.28±10.62 minutes, Bowel preparation quality (Boston Scale): 89.3% 95.7%), patient discharge from the recovery room, Average discharge time post-procedure: With propofol alone: 30.92 minutes; With propofol and fentanyl combined: 31.52 minutes, The intermediate risk was 0.28% by the TROOPS evaluation during procedural sedation. Conclusion The quality benchmark levels for these endoscopic units, as determined by a single-center study, can be effectively implemented by benchmark endoscopy centers to enhance their quality and safety operations.

Background: According to the International Labor Organization (ILO), 2.78 million workers are injured and killed each year as a result of occupational diseases, while 374 million are injured. Furthermore, work-related injuries cost the global economy 4% of its GDP. Construction workers are more likely than other workers to die and twice as likely to be injured. Construction accidents are frequently caused by a lack of knowledge and training, negligence, and inadequate safety equipment. Workers account for 70% of the factors influencing accidents, 49% of workplace problems, 56% of equipment shortages, 27% of material conditions, and 84% of risk management. Between 2009 and 2017, 3321 industrial accidents and acute poisoning cases were reported in Mongolia. 509 people died as a result of industrial accidents, 761 people became disabled as a result of injuries, and 2051 people temporarily lost their ability to work. As a result, determining the distribution, causes, and factors of accidents is reasonable. Purpose: To detect the prevalence and influencing factors of accidents in the construction industry in the last 5 years. Material and Method: Secondary data was collected using a quantitative research method based on a single-moment model of observational research from the "General Directorate of Professional Supervision" quantitative data on industrial accidents and acute poisoning by sampling 10 groups and 59 indicators of accident cases in the construction industry. Result: The prevalence of accidents in the construction industry was calculated for 1000 workers in Ulaanbaatar's 21 provinces and 9 districts using the ARC GS program. For the calculation of influencing factors and injury causes at www.graphpad.com, P values less than 0.05 was considered significant.
Between 2017 and 2021, 134 accidents and injuries were reported in Mongolia's construction industry. All accidents were male in 123 cases (92%), and female in 11 cases (8%).
Accidents and injuries in the construction industry are distributed. In Mongolia, Ulaanbaatar had 66 cases, Umnugovi had 10, Orkhon had 10, and Khovd province had 12, all of which had more accidents than other provinces. Calculating the distribution per 1000 workers, Sukhbaatar has 20, Dornogovi has 15, Umnugovi has 12, and Khovd has 10.9.
The most important factors influencing construction accidents are the workplace environment and human factors. In terms of accident causes, falling, tripping, or slipping caused 55 or 41% of all accidents, falling objects caused 24 or 18%, being trapped by objects caused 19 or 14%, and 20 or 15% were remaining causes of injuries. In the last 5 years, 47 or 35.07% of all accidents were caused by workplace environment, 45 or 33.5% by human factors, 23 or 17.1% by management system, and 19 or 14.1% by technical and technological factors. Conclusions
1. In the last five years, the prevalence of accidents among construction workers is 16.3 per 1000. The prevalence is 42.9% (66) highest in Ulaanbaatar City, 20.1% (27) in Khan-Uul district, and 8.9% (12) in Khovd province. The prevalence per 1000 workers is 20 in Sukhbaatar, 15.7 in Dornogovi, 12 in Umnugovi, and 10.9 in Khovd, with a high prevalence of 1.07 in Ulaanbaatar.
2. Human factors, such as falling from a height due to noncompliance with safety rules, limited space in the workplace and environment, and falling into pits, wells, ditches, and excavated pits, are the leading causes of injuries and accidents. Closed fractures and head injuries are the most common.

Assessing children with disabilities using who international classification of functioning (ICF) Background: In 2021, according to the World Health Organization (WHO), over 1 billion people are estimated to experience disability. The number of children with disabilities globally is estimated at almost 240 million, according to a new UNICEF report. There are approximately 43 million children with disabilities in East Asia and the Pacific. In the 2020 population and housing census of Mongolia, a total of 106.4 thousand people with disabilities were counted, of which 7.6 percent or 8.1 thousand children aged 0-14 were counted. People with disabilities lose some of their ability to labor. WHO recommended that assessment of children with disabilities using both ICD and ICF. Thus, we aim to assess children with disabilities who have neurological disease using International Classification of Functioning and evaluate the validity of this classification. Materials and methods: This was a cross sectional analytical study based on NCMCH. Study materials were collected from children and guardians through standard questionnaires. The questionnaire consisted of 2 groups: general information of the participant and indicators of the scope of the D code of the "ICF" to assess the childhood disability. According to the indicators of the D code range, activity limitations and participation restriction, disabilities were evaluated. Each question in the questionnaire was measured on a 5-point Likert scale from 0 to 4. The statistical analysis was performed using R 3.5.1 program. Validity was assessed using the Rasch model for each question. Questionnaire reliability was assessed by Cronbach's alpha test. Results: The study included 32 children aged 2-15 years. Male children were 62.5% of participants, the mean age was 8±3.1 years. Correlation between questions was high (r = 0.79) and reliability was adequate (α=0.94). As a result of Rasch analysis, the mean and standard deviation of the 36 selected parameters were not significantly different from the standardized mean. 3 indicators that did not meet the analysis criteria were removed, and a total of 33 indicators were used to measure childhood disabilities. Mean infit MNSQ was 1.06, mean outfit MNSQ was 0.93. MNSQ of all participants were 1.0 – 2.0. As a result of Rasch analysis, the mean of 33 indicators of disability is -1.6, the standard deviation is 1.2, the upper limit of the mean is 3.6, and the lower limit is -3.4, and the indicator of D code was stable enough to measure disability. The mean code scores were 2.45±1.3. The mean score of disability level of children diagnosed with cerebral palsy was 2.9±1.09, and children hospitalized with seizures and meningitis was 0.5±0.3. Also, the total mean score was 2.61±1.2 in the group with disability and receiving care, and 1.8±0.21 in the group not receiving care, which was a statistically significant difference. Conclusions: Inter-indicator correlation was good and reliability of the questionnaire was adequate in field use of the 38 indicators of the activity limitations and participation restriction of the International Classification of Functioning, Children's Version (ICF-CY) code range “D”. When evaluated by Rasch analysis, 33 questions were evaluated as structural and stable. The International Classification of Functioning can be used to assess children's disabilities. Discussions: Niels Ove Illum et al. (2015) found that The World Health Organization International Classification of Functioning, Disability and Health child and youth version d code data can provide a coherent measure of severity of disability in children across various diagnoses, ages, and genders. Results were similar to our study.

Introduction : In the last years other country scientists told about not only determine infant weights, need to interest correlation between maternal weight, height and infant weight. In our country few research articles posted about anthropometry of obstetrics and gynecology. Our study aim is determine maternal weight, infant weight, placenta weight and assess factors affecting roles on maternal story of “Amgalan” Maternity Hospital in 2014-2015. Goal: The current study aimed at assessing maternal weight, infant weight, placenta weight and evaluating the effect of factors leading to it. Materials and Methods: The data was already collected from “Amgalan” Maternity Hospital using maternal history and record and it was collected measuring general physical characteristics such as body weight and height, infant weight, placenta weight and body circumferences. We used retrospective method and collected statistical data was analyzed using SPSS 21.0 software. Results: Of total 964 study participants aged 18-45. The average age of participants was 29.6 ± 5.8 years old and 49.7% (n=479) was working during pregnancy, 45.7% (n=441) hadn’t works, 4.6% (n=44) was student. The average weight of mothers was 75.4±11.5, weight of infants was 3439.5±456, weight of placenta was 685±129. The following factors affected maternal and infant weights: lower education, working, early and late pregnancy complication. Maternal weight had a low direct correlation with infant weight (r=0.267, p<0.01) and placenta weight (r=0.208, p<0.01). In our study maternal height had a low direct correlation with infant weight(r=0.173, p<0.01) and infant weight had a moderate direct correlation with placenta weight (r=0.376, p<0.01). Conclusions
1. The average maternal weight was 75.4±11.5, infant weight was 3539.5±456, placenta weight was 685±129.
2. The following factors affected maternal and infant weights: lower education, working status, early and late pregnancy complications.
3. Maternal weight had a little direct correlation with infant weight (r=0.267, p<0.01) and placenta weight (r=0.208, p<0.01).