Objective To analyze the incidence of biliary complications in patients with cholecystolithiasis and calculus of common bile duct after laparoscopic common bile duct exploration(LCBDE)with one-stage suture,and explore the high-risk factors of its occurrence,and then put forward targeted prevention strategies.Methods The clinical data of 201 patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture in our hospital from January 2020 to April 2024 were retrospectively analyzed.After 3 months of follow-up,the incidence of postoperative biliary complications was observed,and the clinical data of patients in the biliary complications group and the non-biliary complications group were analyzed.The factors with statistically significant differences were included in the multivariate Logistic regression model to analyze the independent risk factors affecting the occurrence of patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture.Results Among the 201 patients,12 had biliary tract infection,5 had common bile duct stenosis,and 13 had bile leakage.The total incidence of biliary complications was 14.93%(30/201).Multivariate Logistic regression analysis showed that diabetes mellitus((OR)=1.092,95%CI:1.040～1.147),common bile duct diameter<1 cm((OR)=1.097,95%CI:1.053～1.144),stone incarceration at the lower end of common bile duct((OR)=1.120,95%CI:1.062～1.180),Calot triangle adhesion((OR)=1.099,95%CI:1.042～1.158),bile turbidity((OR)=1.082,95%CI:1.043～1.123)and operation time≥2 h((OR)=1.090,95%CI:1.044～1.138)were independent risk factors for patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture(P<0.05).Conclusion The risk of patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture is high.The occurrence of complications is related to diabetes mellitus,common bile duct diameter<1 cm,stone incarceration at the lower end of the common bile duct,Calot triangle adhesion,bile turbidity and operation time≥2 h.Targeted prevention strategies can be formulated clinically to prevent the occurrence of biliary complications.

Objective To analyze the incidence of biliary complications in patients with cholecystolithiasis and calculus of common bile duct after laparoscopic common bile duct exploration(LCBDE)with one-stage suture,and explore the high-risk factors of its occurrence,and then put forward targeted prevention strategies.Methods The clinical data of 201 patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture in our hospital from January 2020 to April 2024 were retrospectively analyzed.After 3 months of follow-up,the incidence of postoperative biliary complications was observed,and the clinical data of patients in the biliary complications group and the non-biliary complications group were analyzed.The factors with statistically significant differences were included in the multivariate Logistic regression model to analyze the independent risk factors affecting the occurrence of patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture.Results Among the 201 patients,12 had biliary tract infection,5 had common bile duct stenosis,and 13 had bile leakage.The total incidence of biliary complications was 14.93%(30/201).Multivariate Logistic regression analysis showed that diabetes mellitus((OR)=1.092,95%CI:1.040～1.147),common bile duct diameter<1 cm((OR)=1.097,95%CI:1.053～1.144),stone incarceration at the lower end of common bile duct((OR)=1.120,95%CI:1.062～1.180),Calot triangle adhesion((OR)=1.099,95%CI:1.042～1.158),bile turbidity((OR)=1.082,95%CI:1.043～1.123)and operation time≥2 h((OR)=1.090,95%CI:1.044～1.138)were independent risk factors for patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture(P<0.05).Conclusion The risk of patients with cholecystolithiasis and calculus of common bile duct after LCBDE with one-stage suture is high.The occurrence of complications is related to diabetes mellitus,common bile duct diameter<1 cm,stone incarceration at the lower end of the common bile duct,Calot triangle adhesion,bile turbidity and operation time≥2 h.Targeted prevention strategies can be formulated clinically to prevent the occurrence of biliary complications.

Objective:To explore the level of tibial growth plate chondrocyte mitophagy in young rats with chronic renal failure (CRF) and its effect on chondrocyte apoptosis.Methods:Male 4-week-old Sprague-Dawley rats were randomly divided into two groups according to random number table method: normal control group ( n=20, intragastric administration with distilled water) and CRF group ( n=20, given adenine suspension 150 mg·kg -1·d -1). All the young rats were sacrificed after continuous gavage for 6 weeks. The length of tibia was measured on X ray film, the width of tibia growth plate was measured and compared on histological section, and the apoptosis rate of chondrocytes in growth plate was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. The growth plate chondrocytes of two groups were isolated and cultured to the third generation in vitro, and the apoptosis rate of chondrocytes was detected by TUNEL assay. The co-localization of mitochondria and autophagy lysosomes in chondrocytes was observed by double fluorescence staining. Western blotting was used to detect the level of mitochondrial marker protein translocate of the outer mitochondrial membrane-20 (Tom-20) and autophagy marker light chain-3 protein (LC-3). The mitophagy of growth plate chondrocytes was observed by transmission electron microscope. Results:Compared with the normal control group, the tibia length of CRF group was shorter [(27.32±5.81) mm vs (35.43±3.61) mm, t=5.226, P<0.001], and the relative width of growth plate in histological section was narrower (0.56±0.19 vs 1.00±0.21, t=6.744, P<0.001). The apoptosis rate of chondrocytes in growth plate in CRF group was higher than that in the normal control group (17.2%±4.8% vs 5.1%±3.4%, t=6.505, P<0.001). The apoptosis rate of chondrocytes cultured in vitro in CRF group was higher than that in the normal control group (11.8%±6.2% vs 3.1%±1.2%, t=4.357, P<0.001). The result of double influorescence staining showed that there was co-localization between mitochondria and autophagy lysosomes in CRF group. Western blotting results showed that the levels of LC-3 protein ( t=8.944, P<0.001) and Tom-20 protein ( t=6.708, P<0.001) in CRF group were lower than those in the normal control group. Conclusion:The level of tibial growth plate chondrocyte mitophagy in young rats with CRF increases, which will lead to a decrease in the number of mitochondria, an increase in the apoptosis and a decrease in the number of chondrocytes, and eventually lead to dysplasia of tibia.

The particularity and complexity of medical support in the United Nations peacekeeping operations bring various ethical problems to medical staff of the second-grade peacekeeping hospitals during their work.This paper analyzed the ethical dilemma in terms of the relevant provisions of the United Nations,the medical humanitarianism principle,lack of medical resources and the principle of fairness,emergency treatment and informed consent,cultural differences and the principle of optimal health,occupational protection requirements and respect to the human rights.In addition,it discussed the countermeasures from personnel selection,the laws and regulations,the training of medical norms and foreign customs,the concept of “patient-centered”,and the humanitarian treatment,providing a theoretical basis for solving the problems of medical support in peacekeeping operations.

Objective We aimed to analyze the results of physical examinations in bank employees and understand the self-health care consciousness of them in order to establish health education method for the employees.Methods We collected data of physical examinations for 904 bank employees and analyzed the results statisticaUy.Results There were 510 employees who got abnormal results,accounting for 56.4%.The first four diseases were ordered as follows:hyperlipoidemia,hepar adiposum,high blood pressure and hyperuricacidemia.Moreover,the incidence increased with the increasing ages.Besides,the incidence of illness was closely correlated with the employees'living styles. Conclusions We should strengthen the health education and behavior intervention for bank employees in order to urge them to drop off bad living habits self-consciously and establish benign living styles.

Objective To study the transfer of ropivacaine across the single cotyledon of the term human placenta and the effects of maternal hypoproteinemia and fetal acidemia on the transfer. Methods Eighteen placentas were obtained from healthy full term parturients within 5 min after vaginal or cesarean section delivery. The dual perfused human placental model was made. The placentas were randomly divided into three groups of 6 placentas : (A) control group in which 100% fresh frozen plasma was used in both maternal and fetal circulation with pH maintained at 7.4 on both sides; (B) fetal academia group in which 100% fresh frozen plasma was used in both circulations but fetal pH was reduced to 7.0; (C) maternal hypoproteinemia group in which 50% fresh frozen plasma used in maternal circulation and 100% fresh frozen plasma in fetal circulation, pH was maintained at 7.4 on both sides. Samples were taken from the perfusate in the reservoir at 15, 30, 60, 90, 120 min after ropivacaine (2?g?ml-1) and antipyrine (10 ?g?ml-1 ) were added in maternal circulation for determination of concentrations of ropivacaine, antipyrine, glucose and lactate. Glucose consumption rate, lactate generation rate and relative and absolute transfer ratio of ropivacaine were calculated. Results Absolute transfer ratio of ropivacaine was gradually increasing with perfusion time, reaching 8.7?1.0% (A) , 10.5 ?1.6% (B) and 11.8?1.1% respectively at 120 min. Relative transfer ratio of ropivacaine was relatively constant during 120 min perfusion and was significantly higher at each time point in group B and C than in group A ( P

A longitudinal study was carried out on fifty infants selected from both urban and rural areas of Beijing for their body weights, heights, head and mid-upper arm circumferences, tricep and abdomen skinfold thicknesses, and breatmilk and energy and protein intakes at the 1st, 3rd and 6th month of life. The results revealed that the breastmilk intakes of these infants were ranged from 600-800 g/day during this period of time, the breastmilk intakes of the rural infants were higher significantly than that of the urban infant throughout the study period (P