Chronic limb-threatening ischemia(CLTI)is a serious peripheral arterial disease(PAD)characterized by reduced blood flow in the limbs,resulting in tissue damage and dysfunction.Mesenchymal stem cells(MSCs)have become a research hotspot in the field of CLTI treatment in recent years due to their unique regenerative ability and immunomodulatory properties.In the environment of hypoxia and tissue injury,MSCs can promote angiogenesis,reduce inflammation and promote tissue repair by secreting cytokines,cell differentiation and inter-cellular signal transduction,so as to improve the symptoms and prognosis of patients with CLTI,showing a broad clinical application prospect.

To investigate the impact of matrine(Mat)on the immune function of rats with allergic asthma by regulating the Lyn/Syk signal pathway,12 SD rats were randomly selected as control group(NC group),and the other rats were modeled by ovalbumin(OVA)with reference to previous literature.The successfully modeled allergic asthma rats were randomly grouped into model group,Mat group(100 mg/kg Mat),MLR-1023 group(30 mg/kg Lyn/Syk signal pathway activator MLR-1023),and Mat+MLR-1023 group(100 mg/kg Mat+30 mg/kg MLR-1023),with 12 rats in each group.NC group and Model group were given the same amount of physiological saline once a day for 4 weeks.Bronchoalveolar lavage fluid(BALF)were collected and eosinophils were counted;the level of cytokines in serum and BALF were detected by ELISA kit;the pathological change of lung tissue was evaluated by HE staining;the histamine level in lung tissue was measured;Western blot was applied to detect the levels of Lyn/Syk pathway related proteins.Data showed that the lung tissue in NC group was stained clearly and the structure was normal.In the model group,a large number of inflammatory cells infiltrated,the eosinophils around the bronchus increased,epithelial cells enlarged and fell off.Compared with NC group,the model group demonstrated obvious increase in the number of eosinophils,the levels of TNF-α,IL-4,IL-5,IL-1β and LTD-4 in serum,the levels of IgE and OVA sIgE in BALF,and the levels of histamine,p-Lyn/Lyn and p-Syk/Syk in lung tissue(P<0.05).Matrine could reverse these pathological changes mentioned above in model rats(P<0.05),while MLR-1023 eliminated the therapeutic effect of Mat on allergic asthma rats.Taken together,Mat may improve the immune function of rats with allergic asthma by down-regulating the Lyn/Syk signal pathway.

Objective:To study the improving effect of curcumin on cardiac function and its influence on myocardial collagen concentration in rats with myocardial infarction (MI).Methods:A total of 60 healthy adult male SD rats were randomly divided into sham operation group (n=10,survived rats n=9)and operation group [n=50,left an-terior descending artery was ligated,they were randomly divided into following four groups,on 28 d the treatment and survived rats in every group were:myocardial infarction (MI)group (n=9),control group (received intraper-itoneal injection of mixed solution,n= 10)and curcumin group (received intraperitoneal injection of curcumin, 100mg·kg-1 · d-1 n= 12)].After four weeks,M-mode echocardiography was used to measure left ventricular shortening fraction (LVFS),left ventricular ejection fraction (LVEF)and heart rate (HR).Masson staining was used to detect the myocardial fibrosis alteration after MI.Immunohistochemistry was used to measure expressions of myocardial collagen type Ⅰ and Ⅲ,and ratio of collagen Ⅰ/Ⅲ.Results:Compared with MI group and control group,after four weeks,there were significant rise in LVFS [(17.23±1.97)%,(19.34±0.83)% vs.(26.70± 1.15)%]and LVEF [(42.08±5.50)%,(41.63± 1.81)% vs.(56.76±2.49)%],significant reductions in HR [(433.16±20.05)beats/min,(433.04±24.17)beats/min vs.(403.96±7.08)beats/min],concentrations of myo-cardial collagen Ⅰ and Ⅲ,and ratio of collagen Ⅰ/Ⅲ [(13.5±0.9),(13.9±1.0)vs.(10.3±1.6)]in curcumin group,P <0.01 all.Masson staining indicated that acute myocardial infarction can cause significant left ventricular interstitial fibrosis.Conclusion:Curcumin could significantly improve cardiac function and inhibit myocardial fibro-sis level in rats with acute myocardial infarction.