Objective:To investigate the effects of calf spleen extract(CSE)on immune function,serum ferritin levels,and adverse reactions in patients with esophageal squamous cell carcinoma undergoing adjuvant chemotherapy after surgery,and to verify its effects on esophageal cancer cell proliferation and apoptosis in vitro.Methods:A total of 96 postoperative chemotherapy patients with esophageal squamous cell carcinoma at Tianjin Cancer Hospital Airport Hospital were selected and randomly divided into control group and treatment group.They were treated with TP regimen as routine,and then treated with CSE in the treatment group.After the treatment was completed,adverse reactions and clinical reactions were observed in the two groups,and immune related indicators and ferritin levels were compared before and after treatment.Compared the disease free survival(DFS)differences between two groups of pa-tients during a follow-up period of 1 and 3 years.Using MTT assay to determine the IC50 value of CSE in esophageal cancer cells.Plate cloning method was used to detect the changes in the proliferation ability of esophageal squamous cell carcinoma cells induced by CSE.Comet assay detected DNA strand damage in esophageal cancer cells caused by CSE.Flow cytometry was used to detect the effect of CSE on apoptosis of esophageal cancer cells.Results:Comparison between the two groups of patients before and after treatment showed that CSE had a significant effect on CD3+T,CD4+T,CD8+T,CD4+T/CD8+T,Iron protein levels had an impact.CSE could alleviate the adverse reactions of postoperative chemotherapy in patients with esophageal cancer,and improve their quality of life and clinical symp-toms.CSE treatment could improve short-term DFS in postoperative patients with esophageal cancer.The IC50 concentration of CSE in esophageal cancer cells was 3.018 mg/ml;CSE could inhibit the proliferation of esophageal cancer cells and increase DNA strand dam-age in esophageal cancer cells.CSE can increase apoptosis of esophageal cancer cells.Conclusion:CSE can inhibit the proliferation of esophageal cancer cells,promote their apoptosis,and improve the quality of life and short-term DFS of esophageal cancer patients after treatment by regulating immune function and reducing adverse reactions.

Objective:To investigate the effects of calf spleen extract(CSE)on immune function,serum ferritin levels,and adverse reactions in patients with esophageal squamous cell carcinoma undergoing adjuvant chemotherapy after surgery,and to verify its effects on esophageal cancer cell proliferation and apoptosis in vitro.Methods:A total of 96 postoperative chemotherapy patients with esophageal squamous cell carcinoma at Tianjin Cancer Hospital Airport Hospital were selected and randomly divided into control group and treatment group.They were treated with TP regimen as routine,and then treated with CSE in the treatment group.After the treatment was completed,adverse reactions and clinical reactions were observed in the two groups,and immune related indicators and ferritin levels were compared before and after treatment.Compared the disease free survival(DFS)differences between two groups of pa-tients during a follow-up period of 1 and 3 years.Using MTT assay to determine the IC50 value of CSE in esophageal cancer cells.Plate cloning method was used to detect the changes in the proliferation ability of esophageal squamous cell carcinoma cells induced by CSE.Comet assay detected DNA strand damage in esophageal cancer cells caused by CSE.Flow cytometry was used to detect the effect of CSE on apoptosis of esophageal cancer cells.Results:Comparison between the two groups of patients before and after treatment showed that CSE had a significant effect on CD3+T,CD4+T,CD8+T,CD4+T/CD8+T,Iron protein levels had an impact.CSE could alleviate the adverse reactions of postoperative chemotherapy in patients with esophageal cancer,and improve their quality of life and clinical symp-toms.CSE treatment could improve short-term DFS in postoperative patients with esophageal cancer.The IC50 concentration of CSE in esophageal cancer cells was 3.018 mg/ml;CSE could inhibit the proliferation of esophageal cancer cells and increase DNA strand dam-age in esophageal cancer cells.CSE can increase apoptosis of esophageal cancer cells.Conclusion:CSE can inhibit the proliferation of esophageal cancer cells,promote their apoptosis,and improve the quality of life and short-term DFS of esophageal cancer patients after treatment by regulating immune function and reducing adverse reactions.

Objective:This study aims to analyze the current status and risk factors of suicidal ideation in inpatients with schizophrenia and develop prediction models to improve the identification of individuals at high risk of suicide attempts.Methods:A total of 252 inpatients with schizophrenia were grouped with a 7∶3 ratio into modeling group ( n=176) and validation group ( n=76). The Beck Scale for Suicide Ideation (BSI), the Hamilton Anxiety Scale (HAMA), the Hamilton Depression Scale (HAMD), the Social Support-Rating Scale (SSRS), the Brief Psychiatric Rating Scale (BPRS), the Pittsburgh Sleep Quality Index (PSQI) were used to assess suicidal ideation, anxiety symptoms, depressive symptoms, social support, psychotic symptoms, and sleep quality, respectively. According to the presence of suicidal ideation, all patients were classified into those with and without suicidal ideation. The difference in each potential risk factor between the two groups was tested using the independent sample t-test and χ 2 test as appropriate. Multivariate Logistic stepwise regression analysis was used to identify independent risk factors of suicidal ideation. The R 4.1.2 software was used to construct the risk prediction model and draw a nomogram to visualize the Logistic regression model. The receiver operating characteristic (ROC) and the area under curve (AUC) were calculated to evaluate the predictability of this model. Internal validation of the model was examined using the Boostrap validation method. Results:Of the 252 inpatients with schizophrenia, 92 patients experienced suicidal ideation, with an incidence of 36.51%. The Logistic regression model revealed that gender (β=0.70, OR=3.66, 95% CI=1.02-3.85), education (β=-0.54, OR=0.85, 95% CI=0.19-0.89), chaperonage (β=1.32, OR=6.72, 95% CI=1.38-10.16), anxiety symptoms (β=0.08, OR=3.98, 95% CI=1.01-1.18), depressive symptoms (β=0.11, OR=28.30, 95% CI=1.07-1.16) were independent risk factors of suicidal ideation in inpatients with schizophrenia (all P?0.05). The AUC of the modeling group and validation group were 0.83 and 0.82, respectively. Conclusion:The incidence of suicidal ideation was high in inpatients with schizophrenia. The Logistic risk prediction model constructed in this study has good predictive efficacy, which can provide a theoretical basis for early identification of individuals at high risk of suicide attempts.

Objective:This study aims to analyze the current status and risk factors of suicidal ideation in inpatients with schizophrenia and develop prediction models to improve the identification of individuals at high risk of suicide attempts.Methods:A total of 252 inpatients with schizophrenia were grouped with a 7∶3 ratio into modeling group ( n=176) and validation group ( n=76). The Beck Scale for Suicide Ideation (BSI), the Hamilton Anxiety Scale (HAMA), the Hamilton Depression Scale (HAMD), the Social Support-Rating Scale (SSRS), the Brief Psychiatric Rating Scale (BPRS), the Pittsburgh Sleep Quality Index (PSQI) were used to assess suicidal ideation, anxiety symptoms, depressive symptoms, social support, psychotic symptoms, and sleep quality, respectively. According to the presence of suicidal ideation, all patients were classified into those with and without suicidal ideation. The difference in each potential risk factor between the two groups was tested using the independent sample t-test and χ 2 test as appropriate. Multivariate Logistic stepwise regression analysis was used to identify independent risk factors of suicidal ideation. The R 4.1.2 software was used to construct the risk prediction model and draw a nomogram to visualize the Logistic regression model. The receiver operating characteristic (ROC) and the area under curve (AUC) were calculated to evaluate the predictability of this model. Internal validation of the model was examined using the Boostrap validation method. Results:Of the 252 inpatients with schizophrenia, 92 patients experienced suicidal ideation, with an incidence of 36.51%. The Logistic regression model revealed that gender (β=0.70, OR=3.66, 95% CI=1.02-3.85), education (β=-0.54, OR=0.85, 95% CI=0.19-0.89), chaperonage (β=1.32, OR=6.72, 95% CI=1.38-10.16), anxiety symptoms (β=0.08, OR=3.98, 95% CI=1.01-1.18), depressive symptoms (β=0.11, OR=28.30, 95% CI=1.07-1.16) were independent risk factors of suicidal ideation in inpatients with schizophrenia (all P?0.05). The AUC of the modeling group and validation group were 0.83 and 0.82, respectively. Conclusion:The incidence of suicidal ideation was high in inpatients with schizophrenia. The Logistic risk prediction model constructed in this study has good predictive efficacy, which can provide a theoretical basis for early identification of individuals at high risk of suicide attempts.

Objective To investigate the correlation between semen parameters with spontaneous abortion in early pregnancy after in vitro fertilization (IVF) assisted pregnancy operation .Methods 220 patients with successful pregnancy after IVF assisted pregnancy operation in the reproductive center of this hospital were selected as the research subjects .Among them ,40 cases of early spontaneous abortion served as the observa-tion group and 180 cases without early spontaneous abortion occurrence as the control group .The related pa-rameters of sperm hyaluronic acid unbinding rate ,sperm nucleoprotein immaturity rate and sperm morphology normal rate were measured and analyzed by adopting the computer assisted sperm analysis (CASA) .Results The sperm hyaluronic acid unbinding rate and sperm nucleoprotein immaturity rate in the observation group were significantly higher than those in the control group ,the difference was statistically significant ( P<0 .05);the sperm morphology normal rate in the observation group was significantly lower than that in the control group ,the difference was statistically significant (P<0 .05);the other semen parameters had no statis-tical differences between the two groups (P>0 .05) .The statistical analysis result showed that the sperm hy-aluronic acid unbinding rate ,sperm DNA fragmentation rate and sperm nucleoprotein immaturity had the posi-tive correlation between each other ,which had the negative correlation with the other indicators .After the stepwise analysis by the binary Logistic regression model ,the results showed that the high risk factors affect-ing spontaneous abortion in early pregnancy after IVF included sperm nucleoprotein immaturity rate and sperm hyaluronic acid unbinding rate .Conclusion The sperm nucleoprotein immaturity rate and sperm hyalu-ronic acid unbinding rate are two high-risk factors inducing early spontaneous abortion after IVF .

Objective To evaluate the efficacy and safety of submucosal injection using endoscopic water-jet in peroral endoscopic myotomy (POEM) for treatment of patients with achalasia of cardia (AC). Methods A retrospective analysis was performed on the data of 126 AC patients undergoing POEM in PLA General Hospital from March 2013 to February 2016. All the 126 patients were divided into two groups, 73 in the water-jet group and 53 in the needle injection group. The time to creating a submucosal tunnel, entire operation time, and incidence of complications were compared between the two groups. Results The time to creating a submucosal tunnel and the entire operation time of the water-jet group were both significantly less than those of the needle injection group (6. 38±0. 94 min VS 13. 81±1. 13 min, P<0. 05;27. 81±5. 76 min VS 70. 25±22. 67 min, P<0. 05). The hospital stay of patients after operation was less in the water-jet group than that in the needle injection group (4. 38±1. 87 days VS 5. 64±1. 83 days, P<0. 05). The incidence of bleeding [5. 5％ (4/73) VS 17. 0％ (9/53), P<0. 05] and fever [12. 3％ (9/73) VS 26. 4％ (14/53), P<0. 05] were lower in the water-jet group than those in the needle injection group. The incidences of perforation and pectoralgia were not significantly different between the two groups ( both P>0. 05 ) . Conclusion Endoscopic water-jet injection is safe and effective in POEM, which effectively shortens the time to creat a submucosal tunnel and the operation time, and reduces the incidences of complications including bleeding and fever.

Objective To explore the clinical significance of colonoscopy follow-up in Chinese Lynch syndrome mismatch repair (MMR) gene mutation carriers.Methods The results of colonoscopy follow-up was analyzed in 194 MMR gene mutation carriers of 50 Lynch syndrome families.The follow-up period was from April 2001 to November 2016.The detection rates of advanced adenomas and colorectal cancers,five-year survival rate and ten year survival rate were compared between 123 patients of regular follow-up group (colonoscopy interval less than two years) and 71 patients of irregular follow-up group (time colonoscopy interval more than two years).T test,chi-square test and Kaplan-Meier method were performed for statistically analysis.Results The incidence of colorectal cancer of irregular follow up group was significantly higher than that of regular follow-up group (57.7％,41/71 vs 22.8％,28/123);and the difference was statistically significant (x2 =24.00,P＜0.01).The average age at diagnosis for colorectal cancer in irregular follow up group was younger than that of regular follow up group ((45.3 ± 1.9) years vs (48.7±1.8) years);and the difference was statistically significant (t=4.10,P＜0.01).In regular follow-up group,28.6％ (8/28) advanced-stage colorectal cancer (TNM Ⅲ or Ⅳ) was found,while in irregular follow up group,73.2 ％ (30/41) advanced-stage colorectal cancer was found,and there was statistically significant difference in pathological stage between two groups (x2 =4.90,P =0.032).The five year and ten-year survival rates of regular follow-up group were 96.2 ％ and 85.1 ％,respectively,which were both higher than those of irregular follow-up group (46.3 ％ and 28.7 ％);and the differences were statistically significant (x2 =13.20 and 14.80,both P＜0.05).The incidence of advanced adenomas of irregular follow up group was significantly higher than that of regular follow-up group (49.3％,35/71 vs 18.7％,23/123);and the difference was statistically significant (x2 =20.10,P＜0.05).The detection rate of advanced adenomas of MMR gene mutation carriers was higher than those without MMR mutation gene (85.4％,35/41 vs 14.6％,6/41);and the difference was statistically significant (x2 =5.20,P＜ 0.05).Conclusion Regular colonoscopy surveillance may decrease the incidence and mortality of colorectal cancer in MMR mutation carriers of Lynch syndrome families,and increase five-year and tenyear survival rates.

Tooth development is a complex process that involves precise and time-dependent orchestration of multiple genetic, molecular, and cellular interactions. Ameloblastin (AMBN, also named "amelin" or "sheathlin") is the second most abundant enamel matrix protein known to have a key role in amelogenesis. Amelogenesis imperfecta (AI [MIM: 104500]) refers to a genetically and phenotypically heterogeneous group of conditions characterized by inherited developmental enamel defects. The hereditary dentin disorders comprise a variety of autosomal-dominant genetic symptoms characterized by abnormal dentin structure affecting either the primary or both the primary and secondary teeth. The vital role of Ambn in amelogenesis has been confirmed experimentally using mouse models. Only two cases have been reported of mutations of AMBN associated with non-syndromic human AI. However, no AMBN missense mutations have been reported to be associated with both human AI and dentin disorders. We recruited one kindred with autosomal-dominant amelogenesis imperfecta (ADAI) and dentinogenesis imperfecta/dysplasia characterized by generalized severe enamel and dentin defects. Whole exome sequencing of the proband identified a novel heterozygous C-T point mutation at nucleotide position 1069 of the AMBN gene, causing a Pro to Ser mutation at the conserved amino acid position 357 of the protein. Exfoliated third molar teeth from the affected family members were found to have enamel and dentin of lower mineral density than control teeth, with thinner and easily fractured enamel, short and thick roots, and pulp obliteration. This study demonstrates, for the first time, that an AMBN missense mutation causes non-syndromic human AI and dentin disorders.
Adult ; Amelogenesis Imperfecta ; genetics ; Cells, Cultured ; China ; Codon ; Dentin ; abnormalities ; ultrastructure ; Female ; Humans ; Male ; Microsatellite Repeats ; Microscopy, Electron, Scanning ; Middle Aged ; Mutation, Missense ; Pedigree ; RNA ; analysis ; Transfection ; Whole Exome Sequencing

Objective To evaluate the efficacy of L?Arabinose for bowel preparation before colonos?copy. Methods A total of 170 patients who underwent colonoscopy were randomized into 2 groups. The ex?perimental group (n=85) used L?Arabinose for bowel preparation, while the control group (n=85) used polyethylene glycol electrolyte solution ( PEG?ELS ) . The degree of comfort, adverse effects, and the visibility during colonoscopy were observed. Results Premedication of L?Arabinose for bowel preparation yielded to more comfort ( U=-4?349,P=0?000) , less adverse effects (χ2=29?27,P=0?000) , and similar visibility during colonoscopy ( U=-0?875,P=0?381) compared with PEG?ELS. Conclusion L?Arabinose is safe, comfortable, and effective for bowel preparation before colonoscopy.

Objective To evaluate the efficacy of premedication of pronase and simethicone before upper gastrointestinal endoscopy. Methods A total of 4 690 patients undergoing upper gastrointestinal en?doscopy from January 2014 to November 2014 were recruited at gastrointestinal endoscopy center in Beijing Military General Hospital. All patients were randomized into 3 groups. The pronase plus simethicone group( n=1 602) took 40 ml mixed solution of pronase, sodium bicarbonate and simethicone orally 20 minutes before endoscopy. The simethicone group( n=1 548) took 40 ml simethicone orally 20 minutes before endoscopy. And the control group( n=1 540) took 10 ml lidocaine hydrochloride mucilage orally 5 minutes before endos?copy. The visibility during gastroscopy was observed. Results Each patient underwent gastroscopy, and no severe adverse event occurred during the procedure. The visibility of 82?3%( n=1 318) of the pronase plus simethicone group, 67?7%( n=1 048) of the simethicone group and 28?1% patients( n=432) of the control group respectively reached grade A or B. The visibility during gastroscopy in the pronase plus simethicone group was higher than that in the simethicone group(χ2=89?42, P=0?000) , while that in the simethicone group was higher than that of the control group(χ2=486?30, P=0?000). Conclusion Premedication of pronase and simethicone can improve the visibility during gastroscopy.