Infectious diseases caused by bacteria,viruses,and other pathogens are a significant global public health challenge.Accurate and efficient detection methods are crucial for the diagnosis and prevention of infec-tious diseases.Nucleic acid detection technologies based on the clustered regularly interspaced short palin-dromic repeats(CRISPR)and CRISPR-associated protein(Cas)systems have begun to be applied in the de-tection of pathogenic microorganisms due to their high sensitivity,high specificity,and rapid simplicity.In re-cent years,various convenient and efficient nucleic acid detection technologies and platforms for pathogenic microorganisms have been derived from the CRISPR/Cas system,further expanding the field of pathogen nu-cleic acid detection.This article summarizes the research progress of pathogen nucleic acid detection technolo-gies based on the CRISPR/Cas system,discusses the value of these detection technologies in the in vitro diag-nosis of pathogens,and provides references for the further development of CRISPR/Cas nucleic acid detection technologies.