Objective:To explore the characteristics of systemic immune microenvironment during the progression of dextran sulfate sodium(DSS)-induced acute ulcerative colitis(UC)induced in mice by Mass cytometry(CyTOF).Methods:Male C57BL/6 mice were randomly divided into control group and model group.The control group was given normal drinking water for 15 d.The mouse in the model group were given 5%DSS in drinking water,which was changed to normal drinking water after 7 days.In the model group,peripheral blood was collected on days 4,9 and 15,respectively.CyTOF was used to detect the expressions of 33 immune cell markers and changes in cell subsets in peripheral blood of mice,and the characteristics of systemic immune microenvironment in mice with acute UC were analyzed.Results:The cluster analysis of 33 kinds of immune cell markers showed that CD45+cells in peripheral blood of mice with DSS induced acute UC were divided into 23 fine subgroups,among which the proportions of B cell subgroup,T cell subgroup and neutrophil subgroup showed significant changes.A further dimensional reduction cluster analysis of T cell subsets found significant differences in the composition and proportion of the 10 identified T cell subsets.Conclusion:The systemic immune micro-environment map of mice with acute UC induced by DSS has been successfully constructed,and heterogeneity has been found in the systemic immune microenvironment of mice with acute UC.The changes and activation degree of T cell subpopulations are closely re-lated to disease progression and inflammation level.The results of this study provide theoretical basis for assisting the diagnosis,moni-toring the risk,progression,treatment and prognosis of acute UC.

Objective:To explore the characteristics of systemic immune microenvironment during the progression of dextran sulfate sodium(DSS)-induced acute ulcerative colitis(UC)induced in mice by Mass cytometry(CyTOF).Methods:Male C57BL/6 mice were randomly divided into control group and model group.The control group was given normal drinking water for 15 d.The mouse in the model group were given 5%DSS in drinking water,which was changed to normal drinking water after 7 days.In the model group,peripheral blood was collected on days 4,9 and 15,respectively.CyTOF was used to detect the expressions of 33 immune cell markers and changes in cell subsets in peripheral blood of mice,and the characteristics of systemic immune microenvironment in mice with acute UC were analyzed.Results:The cluster analysis of 33 kinds of immune cell markers showed that CD45+cells in peripheral blood of mice with DSS induced acute UC were divided into 23 fine subgroups,among which the proportions of B cell subgroup,T cell subgroup and neutrophil subgroup showed significant changes.A further dimensional reduction cluster analysis of T cell subsets found significant differences in the composition and proportion of the 10 identified T cell subsets.Conclusion:The systemic immune micro-environment map of mice with acute UC induced by DSS has been successfully constructed,and heterogeneity has been found in the systemic immune microenvironment of mice with acute UC.The changes and activation degree of T cell subpopulations are closely re-lated to disease progression and inflammation level.The results of this study provide theoretical basis for assisting the diagnosis,moni-toring the risk,progression,treatment and prognosis of acute UC.

Objectives:This study aims to investigate the impact of different Low-Density Lipoprotein cholesterol(LDL-C)levels on progression of intermediate coronary stenosis,and the associated risk factors leading to the progression of such lesions. Methods:Data were collected on 219 consecutive patients admitted at the Fuwai Central China Vascular Hospital from January 2020 to February 2021,underwent angiographic examinations and diagnosed with intermediate coronary stenosis,with at least one follow-up angiography after 11 months.Offline quantitative flow ratio(QFR)analysis was performed on these cases.Patients were divided into two groups:LDL-C controlled group(LDL-C<1.8 mmol/L,148 patients with 191 vessels)and LDL-C uncontrolled group(LDL-C≥1.8 mmol/L,71 patients with 98 vessels).Coronary artery QFR and anatomical indicators such as minimal lumen diameter,minimal lumen area,percentage diameter stenosis,percentage area stenosis were compared within and between the groups.Further analysis was performed to identify influencing factors leading to changes in coronary physiological parameters derived from QFR. Results:Within the LDL-C controlled group,there was no significant difference in the QFR values of the vessels compared to baseline(P>0.05),whereas in the LDL-C uncontrolled group(P<0.05),a notable decline in QFR was observed.Patients in the LDL-C controlled group had lower rates of maximum diameter and area stenosis and higher minimum lumen diameter and area(all P<0.05).Through multifactorial Logistic regression analysis,it was found that a body mass index＞28 kg/m2,LDL-C≥1.8 mmol/L,and a history of myocardial infarction were independent risk factors leading to the decline in QFR(all P<0.05). Conclusions:It was found that patients in the LDL-C controlled group had higher coronary artery QFR,minimum lumen diameter and area,lower rates of maximum diameter and area stenosis.

Imaging mass cytometry(IMC)is a new technology integrating mass spectrometry,high-resolution laser ablation and immunohistochemistry/cytochemistry.With a unique high-dimensional perspective to comprehensively and accurately depict the complex phenotypes,signaling pathways,and tumor immune interactions in the tissue and tumor microenvironment,is widely used in gastrointestinal tumors.This article reviews the application of IMC in depicting the panorama of tumor microenvironment,revealing tu-mor spatial heterogeneity,clarifying tumor pharmacological mechanism,assisting new drug development,and evaluating the efficacy of immunotherapy dynamically in digestive system tumors.

Objective To investigate the clinical efficacy of different stents placement under endoscopic retrograde cholangiopancreatography(ERCP)in patients with malignant biliary obstruction(MBO)and the effect on patient survival time.Methods Clinical data of MBO patients treated with ERCP stent placement between January 2020 and March 2024 were collected,divided into recyclable stent group(33 cases),metal stent group(42 cases),and ordinary stent group(34 cases).Comparation of the three groups of preoperative and postoperative changes in liver function,complications of long-term cholangitis and pancreatitis,stent patency time,success rate of stent removal with a single clamping,survival time,monitoring follow-up situation.Results There was no statistically significant difference in the liver function of the three groups of patients before stent placement(P>0.05);One week after stent placement,the difference compared with preoperative between direct bilirubin(DBiL)and total bilirubin(TBiL)in the recyclable stent group and the metal stent group was significantly higher than that in the ordinary stent group,and the difference between the ordinary stent group and other two groups was statistically significant(P<0.05).The incidence of cholangitis in the recyclable stent group was the lowest,followed by the ordinary plastic biliary stent,and the metal biliary stent had the highest incidence of cholangitis,the incidence of cholangitis in the long term after stent placement was compared among the three groups of patients with a statistically significant difference(P<0.05).The incidence of postoperative pancreatitis in the three groups was not statistically significant(P>0.05).The success rate of stent removal with a single clamping was higher in the recyclable stent group than the ordinary stent group.Comparison of median stent patency time among the three groups,the difference was statistically significant(P<0.05).The metal stent group had the longest median patency time of 194.0 d,recyclable plastic stent had the second longest median patency time of 126.0 d,and ordinary plastic biliary stent had the shortest median patency time of 92.0 d.Median survival time among the three groups was statistically significant(P<0.05).The recyclable plastic biliary stent had the longest median survival time of 590.0 d,metal biliary stent had the second longest median survival time of 476.0 d,and ordinary plastic biliary stent had the shortest median survival time of 453.0 d.Conclusion Recyclable plastic biliary stent has a faster decrease in bilirubin index compared with the ordinary stent group after operation.And the recyclable plastic stent group has lower incidence of long-term cholangitis,higher success rate of one-time clamping of the stent,and more advantages in time to stent patency and survival time compared with ordinary plastic biliary stent,which is an effective choice of stenting modality for ERCP stent placement in patients with MBO.

Objective:To evaluate the efficacy of ulnar nerve decompression (cubital and ulnar tunnels) in double crush syndrome (DCS) of the ulnar nerves.Methods:A retrospective analysis was performed on the clinical data of 23 patients with DCS of the ulnar nerves in Peripheral Specialty of Neurosurgery Department, Japan-China Friendship Hospital from June 2018 to December 2022. All patients underwent both cubital tunnel decompression and ulnar tunnel decompression. Efficacy was evaluated by observing improvement of subjective symptoms and objective indicators and ulnar nerve motor function.Results:Follow-up was performed for (25.82±9.3) months, ranged 9-42 months, in these 23 patients. At the final follow-up, 20 patients (86.95%) had numbness relief, all 23 patients (100%) reported pain alleviation, 12 patients (52.17%) had improved muscle atrophy, and 18 patients (78.26%) had improved motor dysfunction;the sensory nerve conduction velocity ([42.13±2.8] m/s) after decompression was significantly increased than that before decompression ([31.74±3.9] m/s), and the motor nerve conduction velocity ([41.57±2.9] m/s) after decompression was significantly increased than that before decompression ([28.39±6.1] m/s, P<0.05); two-point discrimination threshold of the ring finger ([7.43±1.3] mm) after decompression was significantly decreased than that before decompression ([8.48±1.9] mm, P<0.05); and excellent functional recovery rate of the ulnar nerves was 82.6% (19/23). During the follow-up, no recurrence appeared. Conclusion:Clear efficacy of ulnar nerve decompression (cubital and ulnar tunnels) in DCS of the ulnar nerves is noted.

Objective:To evaluate the efficacy of ulnar nerve decompression (cubital and ulnar tunnels) in double crush syndrome (DCS) of the ulnar nerves.Methods:A retrospective analysis was performed on the clinical data of 23 patients with DCS of the ulnar nerves in Peripheral Specialty of Neurosurgery Department, Japan-China Friendship Hospital from June 2018 to December 2022. All patients underwent both cubital tunnel decompression and ulnar tunnel decompression. Efficacy was evaluated by observing improvement of subjective symptoms and objective indicators and ulnar nerve motor function.Results:Follow-up was performed for (25.82±9.3) months, ranged 9-42 months, in these 23 patients. At the final follow-up, 20 patients (86.95%) had numbness relief, all 23 patients (100%) reported pain alleviation, 12 patients (52.17%) had improved muscle atrophy, and 18 patients (78.26%) had improved motor dysfunction;the sensory nerve conduction velocity ([42.13±2.8] m/s) after decompression was significantly increased than that before decompression ([31.74±3.9] m/s), and the motor nerve conduction velocity ([41.57±2.9] m/s) after decompression was significantly increased than that before decompression ([28.39±6.1] m/s, P<0.05); two-point discrimination threshold of the ring finger ([7.43±1.3] mm) after decompression was significantly decreased than that before decompression ([8.48±1.9] mm, P<0.05); and excellent functional recovery rate of the ulnar nerves was 82.6% (19/23). During the follow-up, no recurrence appeared. Conclusion:Clear efficacy of ulnar nerve decompression (cubital and ulnar tunnels) in DCS of the ulnar nerves is noted.

【Objective】 To investigate the establishment of multi-center haemovigilance (HV) and the monitoring of adverse reactions to blood donation (ARBD), in order to provide basis for the management of blood donors. 【Methods】 The operation of HV was investigated by questionnaire. The total number of blood donations (including plateletpheresis) and ARBD cases occurred in each blood center from 2014 to 2018 were analyzed. 【Results】 Among the 24 blood centers in this survey, only nine got HV operated. The incidence of ARBD of 19 blood centers that fulfilled the questionnaire was in the range of (0.003~1.151) %. The change trend of number and incidence of ARBD cases were indeterminate. 【Conclusion】 Most blood centers did not got HV established. The incidence of ARBD varied greatly and was indeterminate. The application of HV should be further improved to strengthen ARBD management.

OBJECTIVE: To develop a self deep breathing training device which can improve lung function compliance and blood oxygen saturation. METHODS: The device consists of four parts:flow tube, measuring cylinder, mobile phone holder and meridian guidance audio-visual synthesis training software. The flow tube measures the flow rate of inhaled gas, the metering cylinder measures the total amount of inhaled gas, and the mobile phone rack is equipped with a mobile phone storing the meridian guidance audio-visual synthesis training software. RESULTS: The device is reasonable in structure and flexible in operation, which can meet the requirements of self deep inspiration training under the guidance of training module. CONCLUSIONS Deep inspiration training under the guidance of guidance training module can form "deep and slow" abdominal breathing, and then improve lung function.
Cell Phone ; Lung ; Meridians ; Software