The disease-syndrome combination animal model in traditional Chinese medicine （TCM） andrology serves as an important bridge linking TCM theory with modern medical research， providing a key experimental platform for elucidating the 'syndrome-disease' correlation mechanism in male-specific diseases and for screening effective prescriptions. This article reviews recent progress in animal model research on common TCM andrological diseases， including prostatic diseases， sexual dysfunction， and male infertility， with a focus on analyzing the application， advantages， and disadvantages of various modeling strategies， such as immune induction， hormonal intervention， and multi-factor combination across different syndrome types. However， despite breakthroughs in model construction techniques， current research still faces several challenges， including insufficient standardization of syndrome differentiation and difficulties in quantifying TCM-specific indicators. Future studies need to optimize model evaluation systems by integrating modern technologies， in order to promote the standardization and internationalization of TCM andrology research.

Glucose metabolic reprogramming is important in promoting the occurrence and development of malignant tumors and meeting the high demands of the malignant proliferation of tumor cells. An in-depth investigation of the mechanism of glucose metabolic reprogramming in lung cancer is important for the development of effective therapeutic strategies for lung cancer. Traditional Chinese medicine (TCM) is characterized by multiple components, targets, and pathways and can exert antitumor effects through multiple mechanisms. This article illustrates systematically the mechanism of action of Chinese herbal monomers and compound formulas regulating glucose metabolic reprogramming in lung cancer and explores deeply their regulatory effects on key enzymes of glycolysis and metabolism-related signaling pathways, and their potential in overcoming the drug resistance of lung cancer. It aims to provide a reference basis for the study of therapeutic targets and mechanisms of action of TCM in prevention and treatment of lung cancer and theoretical sources for the research and development of new medicines.

Objective:To explore the needs and feelings of emergency palliative care from the perspective of medical and nursing staff.Methods:Purposeful sampling was used to recruit 23 emergency medical and nursing staff with experience in end-of-life care from two Class Ⅲ Grade A hospitals in Wenzhou City between September and October 2024. Semi-structured interviews were conducted with the respondents. Results were analyzed using NVivo 12 software.Results:Emergency patients at the end of life and their families had immediate needs, including acute symptom control, pain management, and respiratory distress treatment needs, urgent decision support needs, cultural conflict needs, immediate emotional comfort needs and alexithymia. Medical and nursing staff faced numerous obstacles when providing palliative care in emergency settings, such as role conflicts and skill gaps among emergency medical and nursing staff, lack of dedicated palliative care areas and noisy environments that hindered communication due to physical space limitations in the Emergency Department, short decision-making time and difficulties in referral due to limited emergency room time window, the multidisciplinary collaboration in the emergency department lags behind and the response of the specialized team was delayed, and lack of information sharing between emergency departments and wards.Conclusions:Emergency palliative care is characterized by both urgent needs and implementation barriers. It is necessary to optimize the physical space of the Emergency Department, establish a rapid decision mechanism for medical and nursing staff, strengthen multidisciplinary collaboration, and conduct professional training to build a palliative care model suitable for emergency situations.

Objective:To explore the needs and feelings of emergency palliative care from the perspective of medical and nursing staff.Methods:Purposeful sampling was used to recruit 23 emergency medical and nursing staff with experience in end-of-life care from two Class Ⅲ Grade A hospitals in Wenzhou City between September and October 2024. Semi-structured interviews were conducted with the respondents. Results were analyzed using NVivo 12 software.Results:Emergency patients at the end of life and their families had immediate needs, including acute symptom control, pain management, and respiratory distress treatment needs, urgent decision support needs, cultural conflict needs, immediate emotional comfort needs and alexithymia. Medical and nursing staff faced numerous obstacles when providing palliative care in emergency settings, such as role conflicts and skill gaps among emergency medical and nursing staff, lack of dedicated palliative care areas and noisy environments that hindered communication due to physical space limitations in the Emergency Department, short decision-making time and difficulties in referral due to limited emergency room time window, the multidisciplinary collaboration in the emergency department lags behind and the response of the specialized team was delayed, and lack of information sharing between emergency departments and wards.Conclusions:Emergency palliative care is characterized by both urgent needs and implementation barriers. It is necessary to optimize the physical space of the Emergency Department, establish a rapid decision mechanism for medical and nursing staff, strengthen multidisciplinary collaboration, and conduct professional training to build a palliative care model suitable for emergency situations.

Objective:To study the variational trend in disease characteristics of patients with hepatitis B-related primary liver cancer (HBV-HCC) in the past five years.Method:A single-center retrospective cross-sectional analysis was performed to compare patients diagnosed with HBV-HCC from January 2012 to December 2016 (control group) and from January 2017 to December 2021 (observation group). The data of the study variables were extracted from the electronic medical record system of the hospital information system of the Second Hospital of Lanzhou University. The 1:2 propensity score matching was used to adjust potential confounding factors such as gender and age. Multivariate logistic regression analysis was used to study the factors affecting changes in disease characteristics of the HBV-HCC population in the observation group. GraphPad Prism 8.0 software was used to draw forest plots to intuitively display the effect size of the study variables in the logistic regression analysis.The t-test was used to compare normally distributed data between groups. The χ2 test was used for inter-group comparison. Results:A total of 1 717 eligible cases were collected, including 510 in the control group and 1 207 in the observation group. Compared with the control group, the number of newly diagnosed cases in the observation group increased by 2.36 times, and males were still the main onset population (83.3% vs. 82.7%). The median age of onset increased (51.9 vs. 53.5 years, P<0.001). 79.4% of HBV-HCC patients had not received antiviral therapy, and the proportion of HBeAg-negative patients increased (56.4%). The factors affecting HBV-HCC patients included family history of HBV ( OR=1.626, 95% CI: 1.181-2.238), family history of hepatocellular carcinoma ( OR=1.388, 95% CI: 1.013-1.901), hypoviremia ( OR=1.322, 95% CI: 1.046-1.671), abnormal alanine aminotransferase ( OR=1.545, 95% CI: 1.231-1.940), liver fibrosis ( OR=1.478, 95% CI: 1.153-1.894), liver cirrhosis ( OR=1.431, 95% CI: 1.128-1.815), and metabolic-related fatty liver disease ( OR=1.438, 95% CI: 1.116-1.815) after propensity score matching adjustment. The factors affecting HBeAg-positive patients were decreased ( OR=0.390, 95% CI: 0.389-0.617); however, the number of early HBV-HCC diagnoses was increased (12.7% vs. 19.3%, P=0.001). Conclusion:The characteristics of patient disease and occurrence of HBV-HCC are changing over the past five years. The risk of developing hepatocellular carcinoma in middle- to older male patients with chronic hepatitis B is increasing with familial history of HBV and hepatocellular carcinoma, HBeAg negativity, hypoviremia, abnormal alanine aminotransferase, liver fibrosis, cirrhosis, and metabolic-related fatty liver disease.

Objective To investigate the effect of etimicin combined with thymopeptide α1 on intestinal flora,circulatory function and renal function in patients with sepsis.Methods A total of 80 patients with sepsis treated in Wenzhou Central Hospital from March 2021 to June 2022 were selected and divided into control group and study group according to treatment methods,with 40 cases in each group.The control group was treated with thymopeptide α1 on the basis of conventional treatment,and the study group was treated with etimicin on the basis of control group treatment,and both groups were treated for 2 weeks.The intestinal flora,circulatory function,renal function,mechanical ventilation duration,hospital stay,acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)scores and clinical efficacy of two groups were compared.Results After treatment,Bifidobacterium,central venous pressure(CVP),mean arterial pressure(MAP)and urine volume of patients in both groups were significantly higher than before treatment,Enterobacter,Enterococcus,Staphylococcus,Bacterioid,Obligate anaerobe,Pseudomonas,heart rate,serum creatinine and blood urea nitrogen were significantly lower than before treatment(P<0.05).Bifidobacterium,CVP and MAP of study group were significantly higher than those of control group,and Enterobacter,Enterococcus,Staphylococcus,Bacterioid,Obligate anaerobe,Pseudomonas,heart rate,and scores of APACHE Ⅱ were significantly lower than those of control group(P<0.05).The mechanical ventilation time and hospital stay in study group were significantly shorter than those in control group(P<0.05).The total effective rate of study group was significantly higher than that of control group(χ2=6.275,P=0.012).Conclusion Etimicin combined with thymopeptide α1 in the treatment of sepsis can effectively regulate the intestinal flora and circulatory function of patients,and has little effect on renal function,which is worthy of clinical promotion and application.

Objective:To summarize the etiology, laboratory examinations and clinical features of rhabdomyolysis(RM)in children.Methods:The clinical data of children with RM admitted to the pediatric general ward at Third Hospital of Peking University from January 1st, 2010 to March 31st, 2021 were collected.The clinical characteristics, etiology distribution, laboratory examinations, treatments and prognosis of the children were analyzed.Results:A total of 24 children were included with 16 males and 8 females.The age ranged from 4 to 15 years old, with median age was 13years old.The etiology was exertional diseases in 14 cases(58.3%), non-exertional diseases in ten cases (41.7%, 7 cases of infection and 3 cases of other causes). The average age of exertional RM was(13.50±1.83)years, and that of non-exertional RM was(8.60±3.72)years.There was significant difference( t=3.848, P=0.002). The main clinical symptoms were muscle soreness, abnormal urine color and muscle weakness.Serum creatine kinase(CK)and serum myoglobin were significantly increased.The proportion of CK value moderate to severe increased of exercise RM children was significantly higher than that of non-exertional RM children( P=0.009). All children were treated with hydration and alkalization.Except for one case who died of critical primary disease, the other 23 children had good treatment response, and no rhabdomyolysis was found during the follow-up. Conclusion:The main causes of rhabdomyolysis in children are exercise and infection.Exertional RM is common in elder children.The increasing of CK level caused by exertional RM is more obvious.Active hydration and alkalization measures could avoid the occurrence of acute kidney injury.Most children with RM have good prognosis.

Objective:To analyze the correlation between indocyanine green retention rate at 15 minutes (ICG-R15) and modified Scheuer score in liver tissues of patients with hepatitis B e antigen-positive/negative chronic hepatitis B (CHB), and further explore the indocyanine green clearance test (ICGCT) applied value in judging the progress of CHB-related liver disease.Methods:407 HBeAg (+) / HBeAg (-) CHB inpatients with normal or slightly elevated serum alanine aminotransferase (ALT) [< 2 times the upper limit of normal (ULN)] and modified Scheuer score were collected, and divided into mild liver disease group (M group, 131 cases, modified Scheuer score < G2S2) and progressive liver disease group (A group, 276 cases, modified Scheuer score≥G2 and / or S2). Furthermore, the groups were sub-divided into HBeAg (+) - M group, HBeAg (-) - M group, HBeAg (+) - A group and HBeAg (-) - A group. The correlation between ICG-R15 and modified Scheuer score was analyzed retrospectively. The data were analyzed by SPSS 24.0 software.Results:Basic clinical characteristics: Among the 407 CHB cases with normal or mildly elevated serum ALT, 171 were HBeAg(+) CHB and 236 were HBeAg(-) CHB. The baseline mean serum HBV DNA was higher in HBeAg(+) CHB patients [(6.06 ± 1.95) log10IU/ml] than HBeAg(-) CHB patients [(3.60±1.37)log10IU/ml ( P = 0.000)]. Included patients ICG-R15 detection characteristics: (1) The baseline mean value of ICG-R15 was not statistically significant between the two groups of HBeAg(+) CHB and HBeAg(-) CHB, and was basically within the normal range (< 10%); (2) Comparison of ICG-R15 baseline mean value among the subgroups showed that the patients in the HBeAg(+)-A group/HBeAg(-)-A group were higher than the HBeAg(+)-M group/HBeAg(-)-M group patients, and the difference was statistically significant ( P = 0.013/ P = 0.000). Included patients’ correlation analysis between ICG-R15 and modified Scheuer score: (1) ICG-R15 and modified Scheuer score had shown weak positive correlation with inflammatory activity grade (g) in HBeAg (+) / HBeAg (-) CHB ( r = 0.237, P = 0.002); r = 0.244, P = 0.000); (2) There was a weak positive correlation between ICG-R15 and fibrosis stage (s) in HBeAg (+) / HBeAg (-) CHB ( r = 0. 254, P = 0; r = 0.225, P = 0.001). Included patients ICG-R15 predictive value for the severity of liver histological progression: when the cut-off value of ICG-R15 was 5.1%, the area under the receiver operating characteristic curve from M group to A group was 0.601 ( P = 0.001) for predicting HBeAg (+) / HBeAg (-) CHB patients. Conclusion:ICG-R15 is positively correlated with the modified Scheuer score of liver tissue in HBeAg (+)/HBeAg (-) CHB patients with normal or slightly elevated ALT. In addition, when the cut-off value of ICG-R15 was 10%, it could not accurately reflect the effective hepatocyte reserve function of HBeAg (+) / HBeAg (-) CHB patients with normal or slightly elevated ALT. Importantly, when the cut-off value of ICG-R15 is 4.0% ~ 5.0%, it may have predictive value for liver disease progression to modified Scheuer score ≥ G2 and / or ≥S2 in HBeAg (+) / HBeAg (-) CHB patients with normal or slightly elevated ALT.

Objective:To investigate the value of logistic regression model based on the features of cone-beam breast CT (CBBCT) for the identification of benign and malignant masses in dense breast.Methods:The data of 106 patients (130 masses) with dense breast who underwent contrast-enhanced CBBCT examination and obtained pathological results from May 2011 to August 2020 were retrospectively analyzed as the training set. From August 2020, the data of 49 patients (54 masses) who met the same criteria were prospectively and consecutively collected and used as the validation set. Taking pathological results as the gold standard, the training set was divided into benign and malignant groups. The t-test, χ 2 test and Fisher′s exact test were used to compare the differences in CBBCT image characteristics between the two groups in the training set. A binary logistic regression model was established by multivariate analysis. ROC curves were used to assess the diagnostic efficacy of the model as a whole in the training and validation sets and the diagnostic efficacy of each feature in the model, and the cut-off value of the intensity (ΔCT) value was determined. The H-L method was used to test the goodness of fit of the model. Decision curve analysis (DCA) was drawn to validate the clinical power of the model. Results:Univariate analysis showed that the breast parenchymal background enhancement (BPE), shape, margin, lobulation, spiculation, density, calcifications, ΔCT value, enhancement pattern, non-mass enhancement, ipsilateral increased vascularity (IIV), and peripheral vascular signs had statistical difference between benign group and malignant group ( P<0.05). BPE, margin, ΔCT value and IIV were included in the multivariate analysis, the equation was logit( P′)=-8.510+0.830×BPE+0.822×margin+1.919× ΔCT+1.896 × IIV. The are a under curve of the model in the training set was 0.879 ( P<0.001) and in the validation set was 0.851 ( P=0.001). The are a under curve of BPE, margin, ΔCT value, and IIV in the diagnosis of malignant mass were 0.645, 0.711, 0.712, 0.775 (all P<0.05); the best cut-off value of ΔCT was 50.38 HU. The fit of this model was good ( P = 0.776). The DCA curve showed that when the risk threshold was 0.05-0.97, the net benefit rate was>0, and this model had some clinical value. Conclusion:The logistic regression model based on the features of CBBCT is helpful to distinguish benign and malignant masses in dense breasts.

Objective: To explore the mediating, direct and indirect roles of self-efficacy, social support and medical coping modes with resilience in patients with chronic hepatitis B. Methods: Totally 384 inpatients and outpatients with chronic hepatitis B, without physical ailments or mental problems were selected.The Connor-Davidson resilience scale (CD-RISC), general self-efficacy scale (GSES), medical coping modes questionnaire (MCMQ) and social support rating scale (SSRS) were adopted in this research.The relationship among all variables was explored by path analysis. Results: (1)The total scores of resilience, self-efficacy, social support, confrontation, avoidance and acceptance-resignation in chronic hepatitis B patients were (61.64±15.36), (25.40±5.70), (39.82±7.39), (19.02±3.72), (16.63±2.69) and (8.45±2.94), respectively.(2)Pearson analysis showed that the scores of self-efficacy, medical coping and social support were significantly correlated with resilience(r=-0.26-0.82, P<0.05). (3)Importantly, path analysis showed that self-efficacy(β=0.53), confrontation(β=0.10), avoidance(β=0.17) and acceptance-resignation(β=-0.13) had direct prediction effects on resilience.Additionally, self-efficacy was indirectly related to resilience(β=0.05), and social support also indirectly related to resilience through self-efficacy, confrontation and acceptance-resignation(β=0.18). Conclusion Self-efficacy, social support and medical coping are the important factors which play a direct or indirect role in resilience.