1.Factors associated with anxiety symptoms among Filipino farmers in Central Luzon: An analytical cross-sectional study
Har-li T. Young ; Dina Marie Yalong ; Vinace S. Guinguing ; Van Irish S. Ventilacion ; Merimae S. Villamayor ; Peter Verona G. Villangca ; William M. Manengyao jr. ; Ma. Beatrice M. Vega ; Alina Marea C. Zañ ; o ; Maria Teresa Sanchez-tolosa
Acta Medica Philippina 2025;59(Early Access 2025):1-11
BACKGROUND AND OBJECTIVE
Filipino farmers face unique occupational challenges that increase the risk of mental health issues, particularly anxiety. This study aims to determine the different personal, environmental, socioeconomic, occupational, and psychosocial factors associated with anxiety symptoms among Filipino farmers in Central Luzon.
METHODSChain referral sampling method was used to recruit participants for the study, who underwent screening based on the eligibility criteria. Eligible participants were then asked about anxiety symptoms using the Generalized Anxiety Disorder-7 (GAD-7), while the validated, researcher-constructed Data Collection Tool for Factors Associated with Anxiety Symptoms (DCFAAS) was used to determine the farmers’ exposure to a variety of factors. Microsoft Excel was utilized in computing for frequency and percent distribution of participants, in each factor. Binary logistic regression was used to compute crude and adjusted odds ratio of each factor thru IBM SPSS Statistics®.
RESULTSAmong the 113 eligible farmers enrolled in the study, only 19 (16.8%) experienced anxiety symptoms, with excessive worrying, which was seen among 45 participants (39.8%). The mental health of Filipino farmers was significantly affected by the presence of physical illness (OR = 10.70 [95% CI 1.367, 83.773]) and having relatives affecting work completion (OR = 6.45 [95% CI 1.346, 30.896]).
CONCLUSIONDespite the low prevalence of anxiety symptoms in this study, the findings suggest government policies to improve mental health service access to farmers, to integrate psychosocial support into agricultural programs, and to address family-related work pressures. By addressing these factors, it can improve farmer productivity and promote overall well-being, putting emphasis on the mental health of the Filipino farmers.
Human ; Farmers ; Agriculture ; Generalized Anxiety Disorder ; Gad-7 ; Patient Health Questionnaire
2.Preface for special issue on Future Agriculture.
Chinese Journal of Biotechnology 2025;41(10):1-6
Agriculture, the strategic cornerstone of national long-term stability, is undergoing a fundamental shift from resource-dependent to technology-driven, driven by global food security and ecological conservation needs. Traditional agriculture can no longer sustain the growing food demand. Scientific and technological advancements are fundamental guarantees for ensuring food supply security and are the primary driver for future agricultural development. This special issue compiles the latest research advancements from diverse experts, covering fields such as microbe-driven green agriculture, pesticide technology innovation, intelligent agricultural machinery, smart manufacturing, and molecular design breeding fundamentals. It aims to inspire researchers to explore cutting-edge directions in future agriculture, promote interdisciplinary collaboration and technological integration, and thereby drive innovative breakthroughs and industrial transformation in agricultural modernization.
Agriculture/methods*
;
Crops, Agricultural/genetics*
;
Food Supply
;
Biotechnology
;
Pesticides
3.Soil carbon and nitrogen dynamics affect bacterial and fungal communities and their interactions: a review.
Xinyuan LIU ; Yue LI ; Ziyan WEI ; Zhujun WANG
Chinese Journal of Biotechnology 2025;41(10):3701-3718
The escalating pressure from global population growth, climate change, and resource consumption is intensifying the burden on traditional agricultural production. Against this backdrop, soil degradation and pollution present increasingly severe challenges, creating a vicious cycle with rising food demands. Maintaining soil health and its ecosystem services has thus become a critical prerequisite for achieving sustainable agriculture in the future. This review explores the impacts of soil carbon (C) and nitrogen (N) dynamics on soil microbial communities and their interactions. Soil C and N are key determinants of microbial diversity and community structure, intrinsically linked to soil C/N cycling, crop productivity, and ecological balance. Environmental factors such as nitrogen fertilizer application, organic matter amendment application, litter decomposition, elevated CO2 concentrations, and nitrogen deposition significantly influence soil C and N dynamics. Changes in soil C and N content regulate microbial community dynamics and the synergistic, competitive, and antagonistic interactions among microorganisms. Meanwhile, microbial communities actively respond to alterations in soil C and N availability. The resulting shifts in microbial communities and their interactions subsequently regulate soil C/N cycling and ecosystem stability, ultimately influencing ecosystem functions. By elucidating the mechanisms underlying soil carbon-nitrogen-microbial interactions, this review significantly advances our understanding of soil ecosystem responses and feedback mechanisms in the context of global change, while also providing crucial practical guidance for enhancing soil fertility and promoting sustainable agricultural development through microbial regulation.
Soil Microbiology
;
Nitrogen/metabolism*
;
Carbon/metabolism*
;
Soil/chemistry*
;
Bacteria/growth & development*
;
Fungi/metabolism*
;
Ecosystem
;
Fertilizers
;
Agriculture
4.Research progress and development trend of nanopesticides and RNA pesticides.
Heng QIAO ; Jingyi CHEN ; Qinhong JIANG ; Xiangge DU ; Jie SHEN ; Shuo YAN
Chinese Journal of Biotechnology 2025;41(10):3774-3789
The production of healthy agricultural products has increased the demand for innovative and sustainable plant protection technologies, and the rapid advancement of nanotechnology has brought revolutionary breakthroughs to traditional agriculture. Nanocarrier-based drug delivery systems can not only significantly improve the utilization efficiency of pesticides, achieving enhanced efficacy and reduced application, but also decrease the pesticide residues and environmental pollution. Additionally, they have made breakthrough progress in the stability and persistence of RNA pesticides. This review summarized the research progress on nanopesticides and RNA pesticides, focusing on the mechanisms of nanocarriers in improving pesticide bioactivity and RNA interference (RNAi) efficiency. It also systematically summarized the types of nanomaterials and their applications in pest and disease management and provided an in-depth outlook for the future development of nanopesticides and RNA pesticides, which provided technical support for the high-quality development of agriculture in the future.
Pesticides/chemistry*
;
Nanotechnology
;
Nanostructures
;
RNA
;
Agriculture/methods*
;
RNA Interference
;
Drug Delivery Systems
5.An intelligent recognition method for crop density based on Faster R-CNN.
Xiuhua LI ; Qian LI ; Hanwen ZHANG ; Lu DING ; Zeping WANG
Chinese Journal of Biotechnology 2025;41(10):3828-3839
Accurately obtaining the crop quantity and density is not only crucial for the demand-based input of water and fertilizer in the field but also vital for ensuring the yield and quality of crops. Aerial photography by unmanned aerial vehicles (UAVs) can quickly acquire the distribution image information of crops over a large area. However, the accurate recognition of a single type of dense targets is a huge challenge for most recognition algorithms. Taking banana seedlings as an example in this study, we captured the images of banana plantations by UAVs from high altitudes to explore an efficient recognition method for dense targets. We proposed a strategy of "cut-recognition-stitch" and constructed a counting method based on the improved Faster R-CNN algorithm. First, the images containing highly dense targets were cropped into a large number of image tiles according to different sizes (simulating different flight altitudes), and the Contrast Limited Adaptive Histogram Equalization (CLAHE) algorithm was adopted to improve the image quality. A banana seedling dataset containing 36 000 image tiles was constructed. Then, the Faster R-CNN network with optimized parameters was used to train the banana seedling recognition model. Finally, the recognition results were reversely stitched together, and a boundary deduplication algorithm was designed to correct the final counting results to reduce the repeated recognition caused by image cropping. The results show that the recognition accuracy of the Faster R-CNN with optimized parameters for banana image datasets of different sizes can reach up to 0.99 at most. The deduplication algorithm can reduce the average counting error for the original aerial images from 1.60% to 0.60%, and the average counting accuracy of banana seedlings reaches 99.4%. The proposed method effectively addresses the challenge of recognizing dense small objects in high-resolution aerial images, providing an efficient and reliable technical solution for intelligent crop density monitoring in precision agriculture.
Musa/growth & development*
;
Crops, Agricultural/growth & development*
;
Algorithms
;
Neural Networks, Computer
;
Unmanned Aerial Devices
;
Seedlings/growth & development*
;
Image Processing, Computer-Assisted/methods*
;
Photography
;
Agriculture/methods*
6.Application and prospects of synthetic biology in the genetic improvement of rice.
Luyao TANG ; Yiting WEI ; Yuqing XU ; Yuexing WANG ; Yuchun RAO
Chinese Journal of Biotechnology 2025;41(10):3840-3862
Synthetic biology, recognized as one of the most revolutionary interdisciplinary fields in the 21st century, has established innovative strategies for the genetic improvement of rice through the integration of multidisciplinary technologies including genome editing, genetic circuit design, metabolic engineering, and artificial intelligence. This review systematically summarizes recent research advancements and breakthrough achievements in the application of synthetic biology in the genetic improvement of rice, focusing on three critical domains: yield improvement, nutritional quality fortification, and reinforcement of disease resistance and abiotic stress tolerance. It elucidates that synthetic biology enables precise genomic and metabolic pathway engineering through modular, standard, and systematic approaches, effectively overcoming the limitations of conventional breeding methods characterized by prolonged cycles and restricted trait modification capabilities. The implementation of synthetic biology has facilitated synergistic improvement of multi-traits, thereby providing critical technical references for developing elite rice cultivars with superior productivity and nutritional value. These technological breakthroughs hold significant implications for ensuring global food security and promoting green and sustainable development of agriculture.
Oryza/growth & development*
;
Synthetic Biology/methods*
;
Metabolic Engineering
;
Plant Breeding/methods*
;
Gene Editing
;
Genetic Engineering/methods*
;
Plants, Genetically Modified/genetics*
;
Disease Resistance/genetics*
7.Research progress on nitrogen use efficiency of wheat.
Luokun RONG ; Rongbin ZHAO ; Ping LIN ; Lei WANG
Chinese Journal of Biotechnology 2025;41(10):3907-3917
Nitrogen use efficiency (NUE) is a pivotal indicator for achieving high wheat yields and sustainable resource utilization. This paper reviews recent research advances in the NUE of wheat, emphasizing genotypic variations, physiological mechanisms, molecular regulation, and agronomic management practices. Furthermore, this paper analyzes the critical regulatory nodes in nitrogen uptake, transport, assimilation, and redistribution, summarizes the current research bottlenecks, and makes an outlook on the future research directions. We then propose a strategy integrating emerging biotechnologies with precision agronomic management to enhance both wheat yields and NUE. This review aims to offer a theoretical framework for breeding nitrogen-efficient wheat cultivars and promoting the eco-friendly production of wheat.
Triticum/growth & development*
;
Nitrogen/metabolism*
;
Plant Breeding
8.Epigenetics and precise crop breeding for resistance.
Chinese Journal of Biotechnology 2025;41(10):3918-3938
Epigenetics refers to a heritable phenomenon that dynamically modulates gene expression without altering the DNA sequence, through molecular mechanisms such as DNA methylation, histone modification, non-coding RNA, chromatin remodeling, and RNA modifications. In plants, these modifications are extensively involved in key biological processes, including flowering time, gametogenesis, stress responses, and immune defenses. Over the past few decades, the research on epigenetics has gradually shifted from fundamental studies primarily conducted in Arabidopsis thaliana to investigations in various crop species such as rice and tomato. This transition has revealed the multifaceted roles of epigenetic regulation in shaping agronomic traits. This review integrates current knowledge of epigenetic regulatory mechanisms and their functions in plant responses to both biotic and abiotic stresses. Epigenetic editing tools such as CRISPR-dCas9 enable targeted DNA methylation or histone acetylation. Emerging transformation technologies, including magnetic nanoparticles and virus-based delivery systems, have the potential to overcome the bottlenecks of plant regeneration, offering new possibilities for precise epigenetic editing. In future agriculture, it is essential to further elucidate multi-layered epigenetic regulatory mechanisms at the single-cell level, develop efficient delivery systems, and leverage artificial intelligence to advance the application of epigenetic breeding for sustainable agricultural development.
Epigenesis, Genetic/genetics*
;
Crops, Agricultural/genetics*
;
Plant Breeding/methods*
;
DNA Methylation/genetics*
;
Gene Editing
;
Disease Resistance/genetics*
;
CRISPR-Cas Systems
9.Effect of a capacity building on the knowledge, attitude, and self-efficacy on Nutrition-Sensitive Agriculture (NSA) of the Local Agriculture Planners in CALABARZON: A non-randomized controlled trial
Kim Leonard G. Dela luna ; Maria Theresa M. Talavera ; Leila S. Africa ; Clarissa B. Juanico ; Nancy A. Tandang ; Marison Felicidad R. Dy
Acta Medica Philippina 2025;59(11):18-29
BACKGROUND
Filipino farmers recorded the highest incidence of poverty in the last decade. In addition, a heightened awareness was observed within agriculture and different government agencies to better understand the link between agriculture and nutrition. However, limited studies have been conducted in the Philippines integrating nutritionsensitive agriculture into the Municipal Agricultural Development Plan (MADP).
OBJECTIVESThis study aims to determine the effect of capacity building on the knowledge, attitude, and self-efficacy of municipal agriculturists on Nutrition-Sensitive Agriculture (NSA) in CALABARZON.
METHODSThis study utilized a non-randomized controlled trial design to measure the change in knowledge, attitude, and self-efficacy of the municipal agriculturists on NSA and nutrition sensitivity of the MADP. A total of 57 municipal agriculturists from CALABARZON participated in the capacity building.
RESULTSLocal agriculture planners' knowledge, attitude, and self-efficacy significantly improved after capacity building. In addition, membership of municipal agriculturists to local nutrition committee was significantly associated with higher scores difference in knowledge and self-efficacy after the training. Meanwhile, the score difference in their attitude was significantly related to the tenure of employment in local agriculture planners.
CONCLUSIONCapacity building on the NSA can significantly improve local agriculture planners' knowledge, attitude, and self-efficacy. Thus, the government must support programs and initiatives concerning the NSA, like increasing the capacity of local agriculture planners. This will allow them to integrate nutrition-sensitive agriculture concepts positively into their agriculture development plan.
Human ; Agriculture ; Food Security ; Capacity Building
10.Factors associated with anxiety symptoms among Filipino farmers in Central Luzon: An analytical cross-sectional study.
Har-li T. YOUNG ; Dina Marie YALONG ; Vinace S. GUINGGUING ; Van Irish S. VENTILACION ; Merimae S. VILLAMAYOR ; Peter Verona G. VILLANGCA ; William M. MANENGYAO JR. ; Ma. Beatrice M. VEGA ; Alina Marea C. ZAÑO ; Maria Teresa SANCHEZ-TOLOSA
Acta Medica Philippina 2025;59(16):68-78
BACKGROUND AND OBJECTIVE
Filipino farmers face unique occupational challenges that increase the risk of mental health issues, particularly anxiety. This study aims to determine the different personal, environmental, socioeconomic, occupational, and psychosocial factors associated with anxiety symptoms among Filipino farmers in Central Luzon.
METHODSChain referral sampling method was used to recruit participants for the study, who underwent screening based on the eligibility criteria. Eligible participants were then asked about anxiety symptoms using the Generalized Anxiety Disorder-7 (GAD-7), while the validated, researcher-constructed Data Collection Tool for Factors Associated with Anxiety Symptoms (DCFAAS) was used to determine the farmers’ exposure to a variety of factors. Microsoft Excel was utilized in computing for frequency and percent distribution of participants, in each factor. Binary logistic regression was used to compute crude and adjusted odds ratio of each factor thru IBM SPSS Statistics®.
RESULTSAmong the 113 eligible farmers enrolled in the study, only 19 (16.8%) experienced anxiety symptoms, with excessive worrying, which was seen among 45 participants (39.8%). The mental health of Filipino farmers was significantly affected by the presence of physical illness (OR = 10.70 [95% CI 1.367, 83.773]) and having relatives affecting work completion (OR = 6.45 [95% CI 1.346, 30.896]).
CONCLUSIONDespite the low prevalence of anxiety symptoms in this study, the findings suggest government policies to improve mental health service access to farmers, to integrate psychosocial support into agricultural programs, and to address family-related work pressures. By addressing these factors, it can improve farmer productivity and promote overall well-being, putting emphasis on the mental health of the Filipino farmers.
Human ; Farmers ; Agriculture ; Generalized Anxiety Disorder ; Gad-7 ; Patient Health Questionnaire


Result Analysis
Print
Save
E-mail