Objective To evaluate the effect of intracarotid cold saline infusion (ICSI) on neurological outcomes in canines with cardiac arrest (CA) introduced by severe hypothermia.Methods Restoration of spontaneous circulation (ROSC) after hypothermic CA was induced in 10 Beagle dogs.These 10 dogs were randomly divided into 2 groups (5 each).Dogs in control group were rewarmed using warn water bath,and dogs in experimental group received the bath rewarming plus ICSI for 6 hours to maintain the brain temperature ＜36℃.In both groups,the Neurologic Disability Scores (NDS) were recorded at 24h after the ROSC,and their brains were removed for pathologic analysis using hematoxylin and eosin stain.The brain water content and sl00β of serum level were also measured.Results The water content (79.43％ ± 0.72％ vs.80.79％ ± 1.06％,P＜0.05) and serum level of s100β (119.83 ± 42.93pg/ml vs.329.82 ± 190.39pg/ml,P＜0.05) were significantly lower in experimental group than in the control group.Control group presented obvious pathological damage of the hippocampal pyramidal cells.There was no significant difference in NDS between the two groups.Conclusion ICSI could reduce the production of s100β and pathological brain damage in postarrest hypothermic canines.

Objective To observe the therapeutic effect and safety of lung recruitment combined with lower tidal ventilation on acute lung injury induced by seawater drowning in rabbits. Methods Eighteen New Zealand rabbits were used for the experiment. Acute lung injury model was developed with intractracheal instillation of seawater (2 ml/kg). Then, the animals had been lower tidal ventilation (6 ml/kg) for three hours. The animals with lung recruitment maneuvers were designated as the experimental group (or the RM group), while those without lung recruitment were designated as the control group. For the RM group,continuous positive airway pressure (CPAP) was applied at a pressure of 2.94 kPa for 20 seconds. Then,arterial blood gas analysis, pulmodynamics, and hemodynamic status were made at various time points. Tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) extracted from serum were also measured following treatment. Pulmonary high - resolution chromatography (HRCT) was also made after three hours of ventilation.Results For the RM group, the partial pressure of arterial oxygen (PaO2) at various time points increased significantly after lung recruitment within two hours(P < 0.05). When a comparison was made between the two groups, PaCO2 for the RM group was higher than that of the control group following lung recruitment at 15 min and 30 min respectively with statistical significance (P < 0.05). Airway plateau pressure (Pplat) decreased markedly(P <0.05)and static compliance increased with statistical significance (P < 0.05). Mean arterial pressure for the RM group decreased in 15 min (P < 0. 05), but almost restored to basic level within 1 h.During recruitment, mean arterial pressure decreased and heart rate increased, but came to normal levels after 30- 90 s. Heart rate at 15 min for the RM group was lower than that of the control group (P < 0.05). However,for both groups, no significant differences could be noted in TN F-α and IL-6 (P > 0.05). Conclusions Lung recruitment combined with lower tidal ventilation could significantly improve gas exchange and lung compliance,and alleviate lung injury, which might be a safe and effective method for the treatment of animals with acute lung injury induced by seawater drowning.

Objective To observe the therapeutic effect and safety of lung recruitment combined with lower tidal ventilation on acute lung injury induced by seawater drowning in rabbits. Methods Eighteen New Zealand rabbits were used for the experiment. Acute lung injury model was developed with intractracheal instillation of seawater (2 ml/kg). Then, the animals had been lower tidal ventilation (6 ml/kg) for three hours. The animals with lung recruitment maneuvers were designated as the experimental group (or the RM group), while those without lung recruitment were designated as the control group. For the RM group,continuous positive airway pressure (CPAP) was applied at a pressure of 2.94 kPa for 20 seconds. Then,arterial blood gas analysis, pulmodynamics, and hemodynamic status were made at various time points. Tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) extracted from serum were also measured following treatment. Pulmonary high - resolution chromatography (HRCT) was also made after three hours of ventilation.Results For the RM group, the partial pressure of arterial oxygen (PaO2) at various time points increased significantly after lung recruitment within two hours(P < 0.05). When a comparison was made between the two groups, PaCO2 for the RM group was higher than that of the control group following lung recruitment at 15 min and 30 min respectively with statistical significance (P < 0.05). Airway plateau pressure (Pplat) decreased markedly(P <0.05)and static compliance increased with statistical significance (P < 0.05). Mean arterial pressure for the RM group decreased in 15 min (P < 0. 05), but almost restored to basic level within 1 h.During recruitment, mean arterial pressure decreased and heart rate increased, but came to normal levels after 30- 90 s. Heart rate at 15 min for the RM group was lower than that of the control group (P < 0.05). However,for both groups, no significant differences could be noted in TN F-α and IL-6 (P > 0.05). Conclusions Lung recruitment combined with lower tidal ventilation could significantly improve gas exchange and lung compliance,and alleviate lung injury, which might be a safe and effective method for the treatment of animals with acute lung injury induced by seawater drowning.