BACKGROUND: The protective effect of mesenchymal stem cells (MSCs) on cardiac ischemia-reperfusion (I/R) injury has been widely reported. Dental pulp-derived mesenchymal stem cells (DP-MSCs) have therapeutic effects on various diseases, including diabetes and cirrhosis. This study aimed to determine the therapeutic effects of DP-MSCs on I/R injury and elucidate the underlying mechanism. METHODS: Myocardial I/R injury model mice were treated with DP-MSCs or a miR-19a-3p mimic. The infarct volume, fibrotic area, pyroptosis, inflammation level, and cardiac function were measured. Cardiomyocytes exposed to hypoxia-reoxygenation were transfected with the miR-19a-3p mimic, miR-19a-3p inhibitor, or negative control. Pyroptosis and protein expression in the interferon regulatory factor 8/mitogen-activated protein kinase (IRF-8/MAPK) pathway were measured. RESULTS: DP-MSCs protected cardiac function in cardiac I/R-injured mice and inhibited cardiomyocyte pyroptosis. The upregulation of miR-19a-3p protected cardiac function, inhibited cardiomyocyte pyroptosis, and inhibited IRF-8/MAPK signaling in cardiac I/R-injured mice. DP-MSCs inhibited cardiomyocyte pyroptosis and the IRF-8/MAPK signaling by upregulating the miR-19a-3p levels in cardiomyocytes injured by I/R. CONCLUSION DP-MSCs protected cardiac function by inhibiting cardiomyocyte pyroptosis through miR-19a-3p under I/R conditions.
Animals ; MicroRNAs/metabolism* ; Pyroptosis/genetics* ; Mesenchymal Stem Cells/metabolism* ; Myocytes, Cardiac/cytology* ; Mice ; Male ; Mice, Inbred C57BL ; Dental Pulp/cytology* ; Myocardial Reperfusion Injury/therapy* ; MAP Kinase Signaling System/physiology*

Rivaroxaban is one of the new oral anticoagulants (NOAC) for preventing stroke and systemic embolism in patients with non-valvular atrial fibrillation. It has clear pharmacokinetic parameters, stable plasma concentration, less drug-drug interaction and higher compliance of patients. However, the discrepancy of pharmacokinetics between individuals and drug-induced hemorrhage events frequently occur clinically, therefore the association of gene polymorphism with drug metabolism has become a research hotspot. This article reviews the research progress on pharmacokinetic characteristics of rivaroxaban and its relationship with gene polymorphism, to provide a reference for the individualized rational use of rivaroxaban.

Objective To evaluate the effect of relaxation training on sudden deafness patients with mild or moderate grade.Methods 78 patients were divided into the observation group (38 cases) and the control group (40 cases).Besides usual care and nursing,the observation group was treated with relaxation training daily in the morning and evening.The pure tone audiometry scores before and after nursing intervention and treatment was compared between two groups.Results There was significant difference in threshold level between two groups.Conclusions Relaxation training was an effective nursing intervention for sudden deafness patients with mild-to-moderate grade.

Objective:To find out the data of the micturitions in healthy young people with the remote & mobile voiding diary monitoring system.Methods:Twenty healthy young people were studied and ten of them were female.The ages ranged from 22 to 35 years(the mean age:27.4 years).The females were 22-33 years old(the mean age:26.4 years) and the males 24-35 years old(the mean age:28.4 years).With the remote & mobile voiding diary monitoring system,their voiding information was collec-ted.Through bluetooth,the voiding information was sent to the patient's intelligent cell phone from the collector,then stored directly by intelligent cell phone and wirelessly transmitted to the workstation in the hospital.All of them completed the voiding diaries for 7 days and the data were analyzed.Results:The average micturition of the young healthy people was 5.6 times(3.4-7.4) per 24 hours,in which 5.3(3.4-7.3) times were in the daytime and 0.3(0-1.3)times in the night.The functional voiding vo-lume was 318 mL(66-642 mL).The mean voiding volume in 24 hours was 1 724 mL(1 152-2 415 mL),in which 1 289 mL(786-2 039 mL) was in the daytime and 435 mL(292-805 mL) in the night.The mean drinking volume was 1 022 mL(453-1 721 mL)in the daytime and 7 mL(0-43 mL) in the night.The nocturia index(Ni)was 1.03,the nocturnal polyuria index(NPi) 26%,and the nocturnal bladder capacity index(NBCi) 0.27.Conclusion:The remote & mobile voiding diary monitoring system can help us get the objective voiding information from young health people for the first time.It is reliable,maneuverable and can be widely used in clinical diagnosis.