Objective:To study the clinical significance of plasma D-dimer for extended anticoagulation in the elderly patients with hip fracture after discharge.Methods:The data were retrospectively analyzed of the 355 elderly patients with hip fracture who had been admitted to Emergence Center of Trauma and Department of Orthopedic Surgery, The Third Hospital of Hebei Medical University from January 2016 to November 2020. There were 107 males and 248 females with a mean age of 79.0 years (from 65 to 102 years). There were 179 femoral neck fractures, 159 intertrochanteric fractures, and 17 subtrochanteric fractures. Plasma D-dimer concentrations were detected at admission, before surgery, and 35 days after surgery. Color Doppler ultrasonography of deep veins of lower extremities was performed at admission, before surgery, before discharge, and 35 days after surgery. The patients with deep vein thrombosis (DVT) at the time of discharge should were reexamined 15 days after discharge. Strategies for anticoagulation prevention and treatment were formulated according to the patients' plasma D-dimer, color Doppler ultrasonography results, symptoms and signs. Plasma D-dimer concentrations were compared between patients with thrombosis and without thrombosis at different time points.Results:The plasma D-dimer concentrations for the 355 patients at admission, before surgery and 35 days after surgery were 2.73 (1.72, 5.13) mg/L, 1.31 (0.72, 2.58) mg/L, and 0.49 (0.25, 0.80) mg/L, respectively. The level of D-dimer at 35 days after surgery was significantly lower than that at admission and before surgery ( P<0.05). There were, respectively, 105, 126,191 and 148 patients with DVT at admission, before surgery, before discharge and 35 days after surgery, and the DVT was distal in most cases. The D-dimer concentrations for patients with thrombosis and without thrombosis at 35 days after surgery was 0.64 (0.32, 1.00) mg/L and 0.37 (0.22, 0.68) mg/L respectively, showing a significant difference ( P<0.05). The area under the receiver operating characteristic curve of plasma D-dimer for the diagnosis of DVT at 35 days after surgery was 0.665. Conclusions:Extended anticoagulation and continuous monitoring of plasma D-dimer are very important for elderly patients with hip fracture after discharge. For patients with DVT at discharge, negative plasma D-dimer is a sign for termination of anticoagulation; for patients without DVT at discharge, it is reasonable to extend anticoagulation 35 days after surgery.

Objective: To investigate the association between the genetic polymorphism of ABCB1 C3435T and plasma concentration and adverse reactions of high-dose methotrexate(HD-MTX) chemotherapy in children with acute lymphoblastic leukemia(ALL).Methods: A total of 70 peripheral blood samples were obtained from the children with acute lymphoblastic leukemia for extracting genome DNA.The genetic polymorphism of ABCB1 C3435T locus was examined by using PCR technology and direct sequencing;enzyme-amplified immunoassay (EMIT) was employed to determine the plasma concentration of MTX in 48 h;the clinical data of patients were collected during the HD-MTX chemotherapy, the adverse reactions were statistically analyzed.The association between ABCB1 C3435T genotypes and MTX plasma concentration and adverse reactions were investigated.Results: Genetic polymorphism existed at the SNP of ABCB1 C3435T.At the SNP of ABCB1 C3435T, the percentage of CC, CT and TT genotype in the children with ALL was 31.43%, 47.14% and 21.43%, respectively.The order of C/D ratio of MTX from low to high was CC, CT and TT genotypes, there was a significant association between the gene polymorphism and the C/D ratio of MTX (P<0.05);the order of incidence of oral mucosa damage from low to high was CC, CT and TT genotypes, there was a significant association between the gene polymorphism and oral mucosa damage (P<0.05);the order of incidence of hepatotoxicity from low to high were CC, CT and TT genotypes, the gene polymorphism and hepatotoxicity had a significant association (P<0.05).Conclusion: ABCB1 C3435T genetic polymorphism and C/D ratio of MTX, gastrointestinal side effects and hepatotoxicity after HD-MTX chemotherapy in the children with ALL exhibit significant association.

Objective:To study the antihypertensive effect of the extract from compound Prunella vulgaris L. in spontaneous hy-pertension(SH)rats. Methods:Forty SH rats were randomly divided into the model group,high,middle and low dose groups of ex-tract from vompound Prunella vulgaris L. ,the compound kendir leaves tablets Ⅰ group with 8 ones in each. Non-invasive blood pres-sure measurement was used to detect the SBP and DBP of the SHR rats. Then the serum NO,AngⅡ ,ET-1 and ANP content were measured after the eight-week treatment. The pathological changes were observed after kidney HE staining in the SH rats. Results:Compared with that in the model group,the blood pressure in high-dose treatment group,middle-dose treatment group and the positive model group was significantly decreased(P < 0. 05). The AngⅡ and ET-1 levels in high-dose treatment group,middle-dose treatment group and the positive model group were decreased(P < 0. 01),and NO and ANP contents in serum were significantly increased when compared with those in the model group(P < 0. 01). The pathological examination showed that the pathological changes in the model group were faster than those in all the drug-treatment groups,the pathological changes included glomerular and renal tubular atrophy, glomerular vascular wall thickening and renal tubular epithelial cell degeneration or necrosis. Conclusion:The extract from compound Prunella vulgaris L. can reduce blood pressure of SH rats. The mechanism may be associated with the level reduction of AngII and ET-1 and content elevation of NO and ANP.

Objective To investigate the changes of non-linear dynamics characteristics of electroencephalogram(EEG) in patients with the stagnation of Liver-Qi syndrome.Methods 15 Liver-Qi stagnation syndrome patients and 15 normal persons were paired by sex and age.The data of EEG under three states(eyes closed,eyes opened,and mental arithmetic with eyes opened) were analyzed by the parameter of point-wise correlation dimension(PD2).Results PD2 of the stagnation of Liver-Qi syndrome group increased significantly compared with that of the healthy control group and always maintain high level.The difference of PD2 between two groups was gradually reduced along with the increase of stimulated condition.Conclusion Non-linear dynamic analysis is more appropriate for the study of mental functions changes of stagnation of Liver-Qi syndrome and can be used to investigate the brain mechanism of the stagnation of Liver-Qi syndrome.

ObjectiveTo investigate the characteristics of personality in patients with depression. Methods24 patients with depression and 24 normal controls were measured with Minnesota Multiphasic Personality Inventory (MMPI) software. ResultsCompared with the control, patient with depression showed significantly difference in neuroticism, extraversionn-introversion, anxiety, repression, manifest anxiety scale, ego strength, dependency, dominance (P<0.05). the T score of N and repression is higher than the Chinese usual score. ConclusionThe depression patients show their personality as repression, inferiority, melancholy, anxiety, bad tolerance, overstrung and dependent.

Objective To explore the characteristics of patients with stagnation of liver-qi syndrome using brain functional imaging techniques.Methods10 patients with stagnation of liver-qi syndrome and 20 normal persons whom paired by sex and age were scanned with positron emission tomography(PET).Data analysis was carried out using SPM in MATLAB software.ResultsCompared with normal,some cerebral areas of the patients with stagnation of liver-qi syndrome showed glucose metabolism letdown(P<0.001),including right parietal lobe(postcentral gyrus,BA1/2),temporal lobe(middle temporal gyrus,BA37),frontal lobe(middle frontal gyrus,BA9/10)etc.,while some cerebral areas showed glucose metabolism heighten(P<0.001)including right frontal lobe(superior frontal gyrus,inferior fontal gyrus,BA22/28/44),right cingulated gyrus,double midbrain and cerebella,etc.ConclusionThe function of some cerebral areas can be changed undergoing stagnation of liver-qi syndrome.

Functional magnetic resonance imaging (fMRI) is an imaging method of function brain area by stimulate in magnetic resonance facility; it can exactitude reflect the nerve activity level of brain and confirm the function brain area in alive body; it is a new impersonality imagine method to evaluate the cognitive function of human. Cognitive function obstacle is a clinical syndrome of damaging cognitive function by manifold reasons and can be leaded by many diseases in clinic. Studying changed function brain area by fMRI can offer an impersonality and intuition imagine evidence to forepart prevent, diagnose and therapy of the diseases of cognitive function obstacle.

ObjectiveTo investigate the analgesic effect of TL-Ⅰ prescription.MethodsHealth ICR mouses were randomly divided into 6 groups: negative control group, positive control group, Tongtian group, large doses group,middle doses group and small doses group. The pain threshold of mouses were detected with hot plate and acetic acid writhing.ResultsTL-Ⅰ prescription can raise the threshold of pain induced by hot plate and reduce the numbers of writhing induced by acetic acid in mice, which was more significant in large doses group. ConclusionTL-Ⅰ prescription can be an effective analgesic.

ObjectiveTo investigate the sedative effect of TL-Ⅰ prescription.Methods72 healthy ICR mice were randomly divided into 6 groups: normal group, model group, positive control group, large doses Chinese materia medica group, middle doses Chinese materia medica group and small doses Chinese materia medica group. The active behavior of migraine-model-mouse was detected with tail suspension test and the analytical system of animal behavior-spontaneous movement.ResultsTL-Ⅰ prescription could prolong the quiescent time of migraine-model-mouse and shorten the active journey and time in the autonomic active box of migraine-model-mouse.ConclusionTL-Ⅰ prescription has sedative effect.

Objective:To explore the effect of Naoshuning on the protein expression of Matrix metalloproteinases(MMPs) in experimental injuried brain tissue of rats. Methods:Immunohistochemistry was used to determine the changes of protein expression of MMPs. Brain tissue water content,permeability and ultramicrostructure of blood-brain barrier(BBB) were also observed. Results:Compared with the sham group,the brain tissue water and EB content of injured side and the level of MMP-2 and MMP-9 protein expression in brain tissue around contusion in model group increased obviously(all P