Objective:To investigate the dosimetric influence of scattering dose mode of border distance on treatment plan when designed CyberKnife(CK)treatment plan for patients with metastatic spinal carcinoma.Methods:The located imaging data of 20 patients with metastatic carcinoma of thoracic vertebrae from radiotherapy of the 940th Hospital of People's Liberation Army Joint Service Support Force between January 2022 and March 2024 were retrospective selected.Using the G4 CyberKnife(CK)treatment plan system MultiPlan 4.0.3 to design respectively CK treatment plans for planning target volume(PTV)and gross tumor volume(GTV).They were divided into PTV group[20 cases who adopted conventional plan method on spinal cord of organs at risk(OAR)in limited dose],and GTV group(20 cases who adopted scattering dose mode of border distance on OAR spinal cord in limited dose).Finally,the prescription dose line was normalized to the PTV.The dosimetric parameters of the target volumes and normal tissues,as well as the distribution of dose curves,were compared between the two groups.Results:The GTV group showed a significant advantage in coverage rate(91.57%±8.73%)of target volume,which was significantly higher than(83.23%±16.05%)of PTV group,with a statistically significant difference(t=3.44,P<0.05).The conformity index(CI)values of GTV group and PTV group were respectively(1.07±0.35)and(1.14±0.04),and there was statistically significant difference between two groups(t=14.69,P<0.05),which indicated better conformity in the GTV group.The distance dose gradient index(DDGI)values per unit distance of GTV group and PTV group were respectively(89.43±46.12)mm and(152.07±109.81)mm,and the difference of that was significant between two groups(t=4.89,P<0.05),the gradient of dropping dose in normal tissue of GTV group had more advantages.The homogeneity index(HI)values of the dose within target volume in GTV group and PTV group were respectively(1.55±0.84)and(1.42±0.20),which also showed a statistically significant difference(t=24.37,P<0.05),and the dose gradient within target volume of GTV group was higher.At the same time,the differences of the parameters of absorbed dose within GTV target volume included D2,Dmean and D95 between two groups were significant(t=14.96,4.71,3.03,P<0.05),and the absorbed dose within GTV target volume in GTV group was higher.Additionally,there were significant differences in the parameters of absorbed dose within PTV target volume included D2,Dmean,D95(cGy)between two groups(t=8.27,5.51,2.50,P<0.05).Within the PTV target volume,the absorbed doses of GTV group was higher than that of PTV group.There were not statistically significant differences between the two groups in terms of the exposure dose of spinal cord,monitor units(MU),total treatment time(times),and total number of beams(beams)(P>0.05).Conclusion:When designing CK treatment plans,the plan that uses GTV as target to utilize scattering dose mode of border distance to design plan in GTV group is better than the plan in PTV group that uses PTV as target in coverage rate of target volume,CI,DDGI,HI and absorbed dose of target volume,which is better design mode of CK plan.

Objective:To investigate the dosimetric influence of scattering dose mode of border distance on treatment plan when designed CyberKnife(CK)treatment plan for patients with metastatic spinal carcinoma.Methods:The located imaging data of 20 patients with metastatic carcinoma of thoracic vertebrae from radiotherapy of the 940th Hospital of People's Liberation Army Joint Service Support Force between January 2022 and March 2024 were retrospective selected.Using the G4 CyberKnife(CK)treatment plan system MultiPlan 4.0.3 to design respectively CK treatment plans for planning target volume(PTV)and gross tumor volume(GTV).They were divided into PTV group[20 cases who adopted conventional plan method on spinal cord of organs at risk(OAR)in limited dose],and GTV group(20 cases who adopted scattering dose mode of border distance on OAR spinal cord in limited dose).Finally,the prescription dose line was normalized to the PTV.The dosimetric parameters of the target volumes and normal tissues,as well as the distribution of dose curves,were compared between the two groups.Results:The GTV group showed a significant advantage in coverage rate(91.57%±8.73%)of target volume,which was significantly higher than(83.23%±16.05%)of PTV group,with a statistically significant difference(t=3.44,P<0.05).The conformity index(CI)values of GTV group and PTV group were respectively(1.07±0.35)and(1.14±0.04),and there was statistically significant difference between two groups(t=14.69,P<0.05),which indicated better conformity in the GTV group.The distance dose gradient index(DDGI)values per unit distance of GTV group and PTV group were respectively(89.43±46.12)mm and(152.07±109.81)mm,and the difference of that was significant between two groups(t=4.89,P<0.05),the gradient of dropping dose in normal tissue of GTV group had more advantages.The homogeneity index(HI)values of the dose within target volume in GTV group and PTV group were respectively(1.55±0.84)and(1.42±0.20),which also showed a statistically significant difference(t=24.37,P<0.05),and the dose gradient within target volume of GTV group was higher.At the same time,the differences of the parameters of absorbed dose within GTV target volume included D2,Dmean and D95 between two groups were significant(t=14.96,4.71,3.03,P<0.05),and the absorbed dose within GTV target volume in GTV group was higher.Additionally,there were significant differences in the parameters of absorbed dose within PTV target volume included D2,Dmean,D95(cGy)between two groups(t=8.27,5.51,2.50,P<0.05).Within the PTV target volume,the absorbed doses of GTV group was higher than that of PTV group.There were not statistically significant differences between the two groups in terms of the exposure dose of spinal cord,monitor units(MU),total treatment time(times),and total number of beams(beams)(P>0.05).Conclusion:When designing CK treatment plans,the plan that uses GTV as target to utilize scattering dose mode of border distance to design plan in GTV group is better than the plan in PTV group that uses PTV as target in coverage rate of target volume,CI,DDGI,HI and absorbed dose of target volume,which is better design mode of CK plan.

Rapid and accurate identification and effective non-drug intervention are the worldwide challenges in the field of depression. Electroencephalogram (EEG) signals contain rich quantitative markers of depression, but whole-brain EEG signals acquisition process is too complicated to be applied on a large-scale population. Based on the wearable frontal lobe EEG monitoring device developed by the authors' laboratory, this study discussed the application of wearable EEG signal in depression recognition and intervention. The technical principle of wearable EEG signals monitoring device and the commonly used wearable EEG devices were introduced. Key technologies for wearable EEG signals-based depression recognition and the existing technical limitations were reviewed and discussed. Finally, a closed-loop brain-computer music interface system for personalized depression intervention was proposed, and the technical challenges were further discussed. This review paper may contribute to the transformation of relevant theories and technologies from basic research to application, and further advance the process of depression screening and personalized intervention.
Humans ; Algorithms ; Depression/therapy* ; Music ; Music Therapy ; Electroencephalography ; Wearable Electronic Devices

Tigecycline is a novel glycylcycline antibacterial drug, which shows both antibiotic function and anti-tumor activity. This review summarizes the single and combined use of tigecycline for tumor treatment and the underpinning mechanisms. As an inhibitor for mitochondrial DNA translation, tigecycline affects the proliferation, migration, and invasion of tumor cells mainly through inhibiting mitochondrial protein synthesis and inducing mitochondrial dysfunction. Although the effect of tigecycline monotherapy is controversial, the efficacy of combined use of tigecycline is satisfactory. Therefore, it is important to explore the molecular mechanisms underpinning the anti-tumor activity of tigecycline, with the aim to use it as a cheap and effective new anti-tumor drug.
Anti-Bacterial Agents/pharmacology* ; Humans ; Minocycline/pharmacology* ; Mitochondria ; Neoplasms/drug therapy* ; Tigecycline/pharmacology*

The prevalence and mortality rate of lung cancer are high. Comprehensive treatment with surgical involvement is the mainstream approach for treating early-stage lung cancer. Video-assisted thoracic surgery has been adopted as the standard minimally invasive surgical option in recent years. However, difficulties in distinguishing cancer tissue, localizing draining lymph node and identifying intersegmental planes make complete removal of cancer tissue challenging. In recent years, near-infrared fluorescence imaging has been integrated with video-assisted thoracic surgery and enjoys broad application prospects. Now it plays an important role in the localization of pulmonary nodules, marking of sentinel lymph nodes and discrimination of intersegmental planes during surgeries.

In order to study the biological function of pig BST-2 gene,the BST-2 gene was amplified with specific primers from porcine kidney tissue,and molecular characterization of BST-2 nuclectide and amino acid sequence were analyzed with bioinformatics tools and online server.Then the prokaryotic expression and tissue expression profile analysis was carried out.The results showed that the full length of pig BST-2 gene was 851 bp and contained 23 bp of 5'-UTR,294 bp of 3'-UTR and 534 bp of CDS and the gene encoded 177 aa.Amino acid sequence analysis of pig BST-2 protein showed 46.1％ identity with gorilla gorilla,41.7％ with cricetulus griseus,39.5％ with mus musculus,35.4％ with equus asinus,42.0％ with felis catus,40.5％ with bos mutus,44.4％ with macaca mulatta,38.7％ with ovis aries and 46.8％ with homo sapiens.BST-2 protein contained 2 transmembrane structure (27-49 aa and 154-176 aa),2 glycosylation sites and 14 potential phosphorylation sites including ATM,CK Ⅱ,PKA,PKC binding sites.The pig BST-2 protein was expressed in Vero cells after translated the recombinant plasmid FLAG-BST-2.Semiquantitative PCR results showed that BST-2 gene was expressed in all the tissues,especially in lymph nodes,thymus,tonsils,spleen,large intestine and small intestine.This study provide a foundation for further understanding the antiviral mechanism of pig BST-2 protein.

With the development of civil military integration,military scientific research institu tes are facing the challenge of constructing a new mode of translating scientific and technological achievements into practice and enhancing translational efficiency.This paper began with the evolution of translation mode in military institutes and discussed the flaws and insufficiency of current mode,then a triple helix translation mode,which encompass government,industry and research,was introduced and fully explained for future reference.

MicroRNA (miRNA for short) can induce mRNA cleavage or inhibit mRNA translation and regulate gene expression in the post-transcriptional level, which involves cell development, proliferation, differentiation, apoptosis, and a series of important biological processes. Recent studies have found that the abnormal regulation of miRNA's target genes may be involved in tumor resistance. And it is expected to become important tumor resistance-associat-ed molecular markers and therapeutic targets. Mechanism of traditional Chinese medicine (TCM) and its active ingre-dients can affect miRNA to regulate target proteins and target genes mediated tumor multidrug resistance. It can pro-vide new ideas for the mechanism of reversing multidrug resistance by TCM.

To investigate the effects of Jianpi Jiedu Formula (JPJDF), a compound Chinese herbal medicine, on vascular endothelial growth factor (VEGF) expression induced by Helicobacter pylori (Hp) in human gastric cancer cells, and to explore the possible mechanism.

Background and purpose: Vascular endothelial growth factor (VEGF) is an important proangiogenic factor, and Helicobacter pylori (H. pylori) infection-induced gastric over-expression of VEGF is an important factor of gastric cancer growth and metastasis, but its expression mechanism is not clear. Cyclooxygenase-2 (COX-2) is a rapid response protein, which is closely related to the occurrence and development of gastric cancer. Our study was to investigate the effect of COX-2 on H. pylori-induced VEGF expression in human gastric cancer cells, and to reveal part of the mechanism of gastric cancer growth and metastasis promoted by H. pylori infection. Methods:The expression ofVEGF mRNA in human gastric epithelial cells (MKN45) infected by standard H. pylori NCTC 11637 and the expression of COX-2 protein were evaluated by real-time fluorogenic quantitative polymerase chain reaction (RFQ-PCR) and assayed by Western blot. After inhibiting COX-2 expression with COX-2 specific inhibitor NS398 (50 μmol/L), VEGF mRNA expression induced by H. pylori in human gastric cancer MKN45 cells was evaluated by RFQ-PCR. Results: H. pylori significantly stimulated the expression ofVEGF mRNA in MKN45 cell line. Compared with control MKN45 cells; VEGF mRNA had 2.33 fold up-regulation after 6 h (P<0.05); and had 5.69 and 5.04 fold upregulation respectively after 12 and 24 h (P<0.01).When MKN45 cells were infected with H. pylori for 24 h, COX-2 protein expression also increased significantly (P<0.01), and after inhibiting the COX-2 expression with COX-2 specific inhibitor NS398, H. pylori-induced VEGF mRNA expression was significantly reduced. Conclusion: H. pylori could induce the expression of COX-2 and VEGF in human gastric cancer cells, and could enhance VEGF expression by COX-2 pathway, which might be one of the important mechanisms of gastric cancer growth and metastasis promoted by H. pylori infection.