Objective To elucidate the molecular mechanisms underlying the effects of Kanggan Mixture(KGM)on key targets in rats with acute lung injury,network pharmacology and in vivo micro-CT experiments were employed.Methods Network pharmacology was utilized to forecast the target genes and principal pathways involved in the intervention of KGM in acute lung injury(ALI).Lipopolysaccharide(LPS)-induced ALI rat models were utilized,and micro-computed tomography(micro-CT)was employed to evaluate the extent of lung injury in vivo.Experiments were conducted to verify the intervention mechanism of KGM on ALI rats.Results The findings revealed that 190 chemical constituents were identified from KGM,and 579 potential targets and 204 pathways associated with KGM's impact on ALI were predicted.The principal components of KGM,such as quercetin,luteolin,kaempferol,betulin,and lupenone,exhibit anti-viral,anti-inflammatory,and immunomodulatory properties by targeting TP53,AKT1,SRC,EP300,and STAT3,and modulating the FoxO signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,and MAPK signaling pathway,demonstrating an influence on acute lung injury.Micro-CT results suggest that KGM can improve lung texture enhancement and lung injury in ALI rats,with an increase in end-expiratory lung volume(inspiratory phase-expiratory phase).The HE and W/D ratio results indicate that KGM can improve lung tissue injury and reduce the lung tissue wet/dry weight ratio(P<0.01).Blood cell analysis results show that the anti-inflammatory agent can decrease the WBC(white blood cell count)and N%(neutrophil percentage)in ALI rats'blood(P<0.01),and increase lymphocytes(P<0.05).Real-time quantitative PCR,WES,and immunohistochemistry results suggest that KGM can decrease the mRNA expression,protein distribution,and protein expression levels of TP53,AKT1,SRC,EP300,and STAT3 in lung tissue of ALI rats(P<0.05).Conclusion KGM has a certain intervention effect on acute lung injury,mainly achieved through the core targets STAT3,EP300,SRC,AKT1,and TP53.

To summarize the nursing experience of a case of adolescent type 1 diabetes with anorexia nervosa.The key points of nursing:to strengthen blood glucose management and be vigilant against hypoglycemia and ketoacidosis;to establish a regular eating pattem to improve nutritional status;to implement personalized graded exercise programs to reduce compulsive exercise behaviors;to carry out phased family therapy to enhance treatment effectiveness;to implement family centered health education to enhance disease management capabilities.After careful treatment and care by a multidisciplinary team,the patient recovered well and was discharged smoothly after 89 days of hospitalization.

Objective To explore the effect of a multicomponent exercise and cognitive stimulation program in elderly patients with mental disorders and sarcopenia,so as to provide references for reducing the risk of falls,preventing and improving sarcopenia,and enhancing cognitive function in patients.Methods The multi-component exercise and cognitive stimulation program was formulated through literature review and expert meeting.In this quasi-experimental study,76 elderly patients with mental disorders and sarcopenia who were hospitalized in a tertiary mental health center in Shanghai from January 2023 to February 2024 were selected as research subjects.They were randomly divided into an experimental group and a control group according to their hospitalization building number(38 cases in each group).The experimental group was treated with multicomponent exercise combined with cognitive stimulation program based on routine nursing,and the control group was treated with routine nursing.The risk of falls,skeletal muscle mass,muscle strength,physical function,cognitive function,and incidence of adverse events were compared between 2 groups after 12 weeks of intervention.Results A total of 75 patients with 37 in the control group and 38 in the experimental group completed the study.The TUG time,6M walking speed and the score of Short Physical Performance Bettery of the experimental group were all lower than those of the control group(P＜0.05),and the scores of skeletal muscle mass,muscle strength,calf circumference,physical function and cognitive function of the experimental group were all higher than those of the control group(P＜0.05).Neither group experienced any adverse events.Conclusion The application of this multicomponent exercise combined cognitive stimulation program developed for elderly patients with mental disorders and sarcopenia can effectively reduce the risk of falls,enhance the skeletal muscle mass and muscle strength and improve the cognitive function in elderly patients with mental disorders and sarcopenia.

Objective To explore the effect of a multicomponent exercise and cognitive stimulation program in elderly patients with mental disorders and sarcopenia,so as to provide references for reducing the risk of falls,preventing and improving sarcopenia,and enhancing cognitive function in patients.Methods The multi-component exercise and cognitive stimulation program was formulated through literature review and expert meeting.In this quasi-experimental study,76 elderly patients with mental disorders and sarcopenia who were hospitalized in a tertiary mental health center in Shanghai from January 2023 to February 2024 were selected as research subjects.They were randomly divided into an experimental group and a control group according to their hospitalization building number(38 cases in each group).The experimental group was treated with multicomponent exercise combined with cognitive stimulation program based on routine nursing,and the control group was treated with routine nursing.The risk of falls,skeletal muscle mass,muscle strength,physical function,cognitive function,and incidence of adverse events were compared between 2 groups after 12 weeks of intervention.Results A total of 75 patients with 37 in the control group and 38 in the experimental group completed the study.The TUG time,6M walking speed and the score of Short Physical Performance Bettery of the experimental group were all lower than those of the control group(P＜0.05),and the scores of skeletal muscle mass,muscle strength,calf circumference,physical function and cognitive function of the experimental group were all higher than those of the control group(P＜0.05).Neither group experienced any adverse events.Conclusion The application of this multicomponent exercise combined cognitive stimulation program developed for elderly patients with mental disorders and sarcopenia can effectively reduce the risk of falls,enhance the skeletal muscle mass and muscle strength and improve the cognitive function in elderly patients with mental disorders and sarcopenia.

To summarize the nursing experience of a case of adolescent type 1 diabetes with anorexia nervosa.The key points of nursing:to strengthen blood glucose management and be vigilant against hypoglycemia and ketoacidosis;to establish a regular eating pattem to improve nutritional status;to implement personalized graded exercise programs to reduce compulsive exercise behaviors;to carry out phased family therapy to enhance treatment effectiveness;to implement family centered health education to enhance disease management capabilities.After careful treatment and care by a multidisciplinary team,the patient recovered well and was discharged smoothly after 89 days of hospitalization.

Objective To elucidate the molecular mechanisms underlying the effects of Kanggan Mixture(KGM)on key targets in rats with acute lung injury,network pharmacology and in vivo micro-CT experiments were employed.Methods Network pharmacology was utilized to forecast the target genes and principal pathways involved in the intervention of KGM in acute lung injury(ALI).Lipopolysaccharide(LPS)-induced ALI rat models were utilized,and micro-computed tomography(micro-CT)was employed to evaluate the extent of lung injury in vivo.Experiments were conducted to verify the intervention mechanism of KGM on ALI rats.Results The findings revealed that 190 chemical constituents were identified from KGM,and 579 potential targets and 204 pathways associated with KGM's impact on ALI were predicted.The principal components of KGM,such as quercetin,luteolin,kaempferol,betulin,and lupenone,exhibit anti-viral,anti-inflammatory,and immunomodulatory properties by targeting TP53,AKT1,SRC,EP300,and STAT3,and modulating the FoxO signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,and MAPK signaling pathway,demonstrating an influence on acute lung injury.Micro-CT results suggest that KGM can improve lung texture enhancement and lung injury in ALI rats,with an increase in end-expiratory lung volume(inspiratory phase-expiratory phase).The HE and W/D ratio results indicate that KGM can improve lung tissue injury and reduce the lung tissue wet/dry weight ratio(P<0.01).Blood cell analysis results show that the anti-inflammatory agent can decrease the WBC(white blood cell count)and N%(neutrophil percentage)in ALI rats'blood(P<0.01),and increase lymphocytes(P<0.05).Real-time quantitative PCR,WES,and immunohistochemistry results suggest that KGM can decrease the mRNA expression,protein distribution,and protein expression levels of TP53,AKT1,SRC,EP300,and STAT3 in lung tissue of ALI rats(P<0.05).Conclusion KGM has a certain intervention effect on acute lung injury,mainly achieved through the core targets STAT3,EP300,SRC,AKT1,and TP53.

Objective:To develop and test the reliability and validity of the questionnaire on knowledge, attitude and practice of hereditary tumor management in nurses, so as to provide a basis for the evaluation of nurses′ knowledge, attitude and practice of genetic tumor managementMethods:This study was a cross-sectional study. According to the theory of knowledge, attitude and practice, the preliminary draft of knowledge, attitude and practice questionnaire for the management of hereditary tumors in nurses was prepared by means of literature review, group discussion, expert correspondence and pre-investigation. From December 2022 to January 2023, 173 nurses from 27 hospitals in Guangdong Province were selected as survey objects by convenience sampling method, and the reliability and validity of the questionnaire were tested to form the final questionnaire.Results:A total of 145 valid questionnaires were collected finally, including 6 males and 139 females aged (31.35 ± 7.23) years old. There were 50 items in the questionnaire of knowledge, attitude and behavior. The Cronbachαcoefficients of the total questionnaire and each dimension was 0.965, 0.938, 0.967, 0.956, and the split half reliability coefficient was 0.780. The total content validity of the questionnaire was 0.960, and the content validity of each item was 0.857 to 1.000. Seven common factors were selected by exploratory factor analysis, and the cumulative variance contribution was 68.72%.Conclusions:The questionnaire on knowledge, attitude and practice of hereditary tumor management in nurses has good reliability and validity. It can be used as a survey tool to study nurses′ knowledge, attitude and behavior of hereditary tumor management.

Objective:To initially construct the index system of core competence for psychiatric mental health advanced practice registered nurses, and clarify the importance of indicators at all levels, so as to provide reference for further training of psychiatric mental health advanced practice registered nurses in China.Methods:A research group was established in October 2021 to determine the content of the index system of core competence for psychiatric mental health advanced practice registered nurses through literature search and discussion of the research group. From November 2021 to February 2022, 18 experts were consulted by Delphi method in the form of email and WeChat to adjust and modify the items.Results:The enthusiasm of experts in the two rounds of expert consultation was 100.0% (18/18) . After two rounds of expert consultation, the index coefficient of variation was less than 0.25. The Kendall 's coefficients of indicators at all levels in the first round of expert consultation were 0.117, 0.158, 0.100 ( P<0.01) , and the Kendall 's coefficients of indicators at all levels in the second round of expert consultation were 0.250, 0.213, 0.189 ( P<0.01) . An index system consisting of 6 first-level indicators, 16 second-level indicators and 56 third-level indicators were formed. Conclusions:The index system of core competence for psychiatric mental health advanced practice registered nurses has a high degree of enthusiasm, authority and coordination of opinions of consulting experts, which can provide a scientific basis for further training psychiatric mental health advanced practice registered nurses in China.

Objective: To describe the post competency level and standardized training of new nurses in Guangzhou tertiary hospitals, and to explore the correlation between them. Methods: By using the questionnaire method, 525 new nurses from 8 tertiary hospitals in Guangzhou were surveyed in June 2016 by convenience sampling, using the general information questionnaire, nursing competency questionnaire and standardized training status questionnaire of new nurses in Guangzhou tertiary hospitals. Results: The competency level of new nurses in Guangzhou tertiary hospitals was 333.53±46.96. The scores of standardized training level were 131.43±23.20. The scores of training management level, training content level and nurses′ belief level were 28.30±5.49, 59.07±14.80, 44.06±2.91. Competency level was positively correlated with standardized training level and each dimension with statistical significance (r=0.099-0.132, P<0.05). Conclusions The post competence of new nurses in Guangzhou tertiary hospitals is at the middle level, and the post competence of new nurses is positively correlated with the level of standardized training. Improving the level of standardized training of new nurses is helpful to enhance the post competence of new nurses. The level of standardized training management, management content and nurse belief can improve the post competence of new nurses.

Objective? To explore and evaluate the effectiveness of the subspecialized fixed tutor training model on the training of the new nurses in the operating room, in order to quickly improve the competency of the new nurses. Methods? A total of 20 new nurses from July 2013 to June 2015 in Sun Yat-sen University Cancer Hospital were selected as the control group. The new nurses from July 2015 to July 2017 were enrolled in the observation group. All the new nurses relied on head nurses and the head supervisory teacher to arrange their training goals and rotation plans. According to the training plan, the control group was randomly assigned to the tutors who were on duty to be responsible for the sub-specialist surgery tour and hand washing training. When the observation team entered a sub-specialty every rotation, the fixed tutor performed one-on-one surgery tour and hand washing training. The scores and comprehensive abilities of the two groups of nurses were compared. The evaluation of the training methods by the new nurses and the satisfaction of the doctors with the new nurses were also compared across the two groups. Results? After the training, the scores of the newly recruited nurses in the theoretical knowledge, operational skills, and surgical coordination were (92.77±3.31), (94.19±2.02), and (95.27±2.55), respectively, and the control group was (90.38±2.63), (88.84±2.44), (90.35±4.92) (t=2.379, 6.891, 3.521, respectively; P＜ 0.05); The observation group's pass rate was 100.0%, and the control group pass rate was 92.5%. The difference was statistically significant (χ2=4.955, P=0.034). After the training, the scores of the new nurses' comprehensive ability in the work initiative, emergency response ability, collaborative communication ability, and learning ability were (9.45±0.21), (9.29±0.33), (9.06±0.34), and (9.08±0.24) in the observation group. The scores were (9.04±0.23), (8.78±0.33), (8.53±0.29), and (8.61±0.39), respectively, in the control group (t=5.332, 4.549, 4.938, 4.185, respectively; P＜0.001). After the training, there was no statistical difference between the two groups in the teaching content of teaching evaluation (P＞0.05). The teaching attitude, teaching method, and training effectiveness scores were higher in the observation group than the control group (t=9.854, 4.758, 9.613, respectively; P＜0.001). After the training, the satisfaction rate of the new nurses in the observation group was 100.0%, and that in the control group was 95.0%. The difference was statistically significant (χ2=12.509, P＜0.05). Conclusions? The subspecialized fixed tutor training model for newly recruited nurses in the operating room is beneficial for the new nurses to grasp the theoretical specialized knowledge in the operating room in a more solid way, to strengthen the practical operation ability of new nurses. Therefore,its implementation is worthy of promoting in the operating room.