Objective The purpose of this study is to investigate the expression of histone lysine-specific demethylase 1 (KDM1A) and ubiquitin carboxyl-terminal hydrolase L3 (UCHL3) in thyroid cancer (TC) tissue and its relationship with clinical features and prognosis. Methods 94 TC patients diagnosed and treated at the Third Hospital of Heilongjiang Province from January 2017 to January 2019 were retrospectively selected as the study subjects. Immunohistochemistry was used to detect the expression of KDM1A and UCHL3 in tissues. Spearman correlation analysis was used to investigate the correlation between KDM1A and UCHL3. Kaplan-Meier survival curve was used to analyze the relationship between the levels of KDM1A and UCHL3 and the 5-year progression-free survival rate of TC patients. Multivariate COX regression model was used to analyze the prognostic factors of TC patients. Results The positive rates of KDM1A (68.09％) and UCHL3 (65.96％) in cancer tissues were higher than those in adjacent tissues(10.64％,8.51％),and the differences were statistically significant(x2=64.984,66.369,all P<0.001). The protein expression of KDM1A and UCHL3 was significant protein correlation (r=0.714,P<0.001). The positive rates of KDM1A (87.50％,90.91％) and UCHL3 (85.00％,87.88％) in TNM stage Ⅲ～Ⅳ and lymph node metastatic TC cancer tissues were higher than those in stage Ⅰ～Ⅱ(53.70％,53.85％)and non-lymph node metastatic(55.74％,54.10％) cancer tissues,and the differences were statistically significant(x2=9.985～12.191,all P<0.001). The 5-year progression-free survival rates of TC patients in the KDM1A positive and negative groups were 62.50％ (40/64) and 86.67％ (26/30),with significant differences,the 5-year progression-free survival rates of UCHL3 positive and negative patients were 58.06％ (36/62) and 90.63％ (29/32),with significant differences (Log-Rankx2=5.670,9.724,P=0.017,0.002). KDM1A positive,UCHL3 positive,TNM stage Ⅲ～Ⅳ,lymph node metastasis were risk factors affecting the prognosis of TC patients (Waldx2=1.315～1.697,all P<0.001). Conclusion KDM1A and UCHL3 are upregulated in TC,and played a pro-cancer role. They are new tumor markers for evaluating the prognosis of TC patients.

Objective The purpose of this study is to investigate the expression of histone lysine-specific demethylase 1 (KDM1A) and ubiquitin carboxyl-terminal hydrolase L3 (UCHL3) in thyroid cancer (TC) tissue and its relationship with clinical features and prognosis. Methods 94 TC patients diagnosed and treated at the Third Hospital of Heilongjiang Province from January 2017 to January 2019 were retrospectively selected as the study subjects. Immunohistochemistry was used to detect the expression of KDM1A and UCHL3 in tissues. Spearman correlation analysis was used to investigate the correlation between KDM1A and UCHL3. Kaplan-Meier survival curve was used to analyze the relationship between the levels of KDM1A and UCHL3 and the 5-year progression-free survival rate of TC patients. Multivariate COX regression model was used to analyze the prognostic factors of TC patients. Results The positive rates of KDM1A (68.09％) and UCHL3 (65.96％) in cancer tissues were higher than those in adjacent tissues(10.64％,8.51％),and the differences were statistically significant(x2=64.984,66.369,all P<0.001). The protein expression of KDM1A and UCHL3 was significant protein correlation (r=0.714,P<0.001). The positive rates of KDM1A (87.50％,90.91％) and UCHL3 (85.00％,87.88％) in TNM stage Ⅲ～Ⅳ and lymph node metastatic TC cancer tissues were higher than those in stage Ⅰ～Ⅱ(53.70％,53.85％)and non-lymph node metastatic(55.74％,54.10％) cancer tissues,and the differences were statistically significant(x2=9.985～12.191,all P<0.001). The 5-year progression-free survival rates of TC patients in the KDM1A positive and negative groups were 62.50％ (40/64) and 86.67％ (26/30),with significant differences,the 5-year progression-free survival rates of UCHL3 positive and negative patients were 58.06％ (36/62) and 90.63％ (29/32),with significant differences (Log-Rankx2=5.670,9.724,P=0.017,0.002). KDM1A positive,UCHL3 positive,TNM stage Ⅲ～Ⅳ,lymph node metastasis were risk factors affecting the prognosis of TC patients (Waldx2=1.315～1.697,all P<0.001). Conclusion KDM1A and UCHL3 are upregulated in TC,and played a pro-cancer role. They are new tumor markers for evaluating the prognosis of TC patients.

In recent years, with the continuous studies on tumor metabonomics, more and more results have shown that changes of metabolism play important roles in the occurrence and development of malignant tumor. Carcinogenic factors can destroy the metabolic balance of human body, induce metabolic reprogramming, and then mediate a variety of biological behaviors to partici-pate in the proliferation and invasion of cancer cells. Lipids provide the body with the necessary energy and essential fatty acids, and a variety of lipid molecules and metabolites are involved in cell signal transduction. Lipid metabolism is an important link in the metabolic system of the body, and the relationship between the occurrence and development of pancreatic cancer and lipid metabo-lism is not clear. The purpose of this paper is to reveal the changes of lipid metabolism in pancreatic cancer, summarize some preclinical studies and clinical trials, and deeply explain the research status of abnormal lipid metabolism associated with pancreatic cancer, so as to provide new ideas for the study of pancreatic cancer pathogenesis and accurate treatment.

Objective To investigate and evaluate the correlation between brain natriuretic peptide (BNP) and gestational hypertension and preeclampsia of hypertensive disorder complicating pregnancy (HDCP).Methods Fifty cases with HDCP and 46 cases with mild and 83 cases with sever stage preeclampsia were selected as our subjects.And 33 cases with regular pregnancy and 31 with irregular pregnancy were served as control group.Plasma brain natriuretic peptide,urinary protein quantity(UBQ),24-hour urinary protein assay (UPA) were measured.The correlations of brain natriuretic peptide and UBQ,UPA,systolic pressure (SP),diastolic pressure (DP) were analyzed.Results The levels of brain natriuretic peptide in the group with gestational hypertension and mild,severe preeclampsia groups were (48.54± 18.27),(79.46± 32.18) and (292.24±213.08) ng/L,higher than that in normal pregnancy and non pregnant group ((27.84± 14.58) and (20.63± 8.28) n/L;F =49.583,P＜0.05).While no significant difference exists between normal pregnancy group and non pregnant group.Grouped on the median values (199) of brain natriuretic peptide of the severe preeclampsia group,the levels of 24-hour UPA,systolic pressure and diastolic pressure were (5.46±2.68) g,(174.55± 13.58) mmHg,(113.74±9.91) mmHg in patients with brain natriuretic peptide ≥ 199 ng/L(n=42),significant higher than those in patients with brain natriuretic peptide ＜ 199 ng/L(n =41;(4.34± 1.95)g,(165.31±11.12) mmHg,(106.05±8.02) mmHg;t=2.603,3.396,2.308;P=＞0.010,0.001,0.024).The levels of 24-hour UPA,systolic pressure and diastolic pressure of patients with brain natriuretic peptide ≥ 86ng/L(n=20) in mild preeclampsia were (1.68±0.27) g,(163.69±8.29) mmHg,(105.45±6.71) mmHg,significant higher than those in patients with brain natriuretic peptide ＜ 86 ng/L (n =26;(1.16 ± 0.31) g,(152.90±7.32) mmHg,(99.19 ± 5.25) mmHg;t =3.180,2.508,2.32;P =0.010,0.016,0.025).Brain natriuretic peptide was closely correlated with UPA,systolic pressure and diastolic pressure in hypertensive disorder complicating pregnancy (HDCP) (r =0.29,0.30;P ＜ 0.01).Brain natriuretic peptide was closely correlated with UPA systolic pressure and diastolic pressure in mild preeclampsia (r =0.39,0.37,0.40;P ＜0.01).And correlation efficacy of brain natriuretic peptide with UPA,systolic pressure and diastolic pressure were 0.44,0.42 and 0.53 (P＜0.01).Conclusion The level of brain natriuretic peptide is closely associated with the severity of gestational hypertension and preeclampsia of hypertensive disorder complicating pregnancy.Correlation of brain natriuretic peptide to the severity of gestational hypertension and preeclampsia is independent of urinary protein and hypertension.Brain natriuretic peptide is an important indicator for the severity of gestational hypertension and preeclampsia of hypertensive disorder complicating pregnancy.

A 55-year-old man with hypertension,type 2 diabetes,abnormal liver function[ alanine aminotransferase(ALT)74 U/L,γ-glutamytransferase(γ-GT)712 U/L)]and hypokalemia(3. 40 mmol/L)received extended release nifedipine 30 mg once daily,benazepril 10 mg once daily,acarbose 25 mg three times a day before each meal,potassium chloride tablets 1. 0 g three time daily,and an IV infusion of compound glycyrrhizin 60 ml(120 mg)once daily. On day 14 after administration the patient developed lower legs weakness and myalgia. The result of examination of animal force of limbs wasⅣlevel. The serum potassium was 1. 1 mmol/L. Compound glycyrrhizin and benazepril were stopped. The patient was given potassium chloride tablets 2. 0 g orally and an IV infusion of 10% potassium chloride 40 ml dissolved in more than 1 000 ml of 0. 9% sodium chloride. The speed of IV infusion was 1. 0 g potassium chloride/hour. The serum potassium was 1. 5 mmol/L four hours after drug withdrawal. The spirolactone 40 mg three times daily was added. Twenty four hours later,the patient′s serum potassium increased to 2. 9 mmol/L,his symptom of myasthenia of limbs improved. Forty eight hours later,his serum potassium was 3. 1 mmol/L,but he developed whole body myalgia,distending pain in both lower extremities and choking sensation in chest. Laboratory test showed creatine kinase( CK)50 940 U/L,CK-MB 441 U/L,lactate dehydrogenase( LDH) 1 992 U/L,aspartate aminotransferase( AST)441 U/L,T( cTnT)0. 20 ng/ml,serum creatinine( SCr) 317 μmol/L,blood urine nitrogen( BUN)18. 2 mmol/L,uric acid( UA)533 mol/L,and carban dioxide-combining Power(CO2-CP)35. 5 mmol/L. Myoglobins in blood and urine did not tested. The color of urine was dark brown. The patient was diagnosed severe hypokalemic,rhabdomyolysis,and renal injury. He received the symptomatic treatments of potassium supplement, fluid infusion, nutritional support, and protection of renal function. On day 5 of compound glycyrrhizin withdrawal,the laboratory test showed the following values:serum potassium 3. 3 mmol/L,CK 10 292 U/L,Cr 252 μmo/L,BUN 16. 3 mmol/L,UA 472 μmo/L;on day 10 of compound glycyrrhizin withdrawal,the laboratory test showed the following values:serum potassium 4. 6 mmol/L,CK 107 U/L,Cr 116 μmo/L,BUN 6. 4 mmol/L,UA 189 μmol/L. His blood pressure was maintained at 130-160/70-90 mmHg,and the symptoms of myasthenia of limbs,myalgia in whole body disappeared completely.

A 55-year-old man with hypertension,type 2 diabetes,abnormal liver function[ alanine aminotransferase(ALT)74 U/L,γ-glutamytransferase(γ-GT)712 U/L)]and hypokalemia(3. 40 mmol/L)received extended release nifedipine 30 mg once daily,benazepril 10 mg once daily,acarbose 25 mg three times a day before each meal,potassium chloride tablets 1. 0 g three time daily,and an IV infusion of compound glycyrrhizin 60 ml(120 mg)once daily. On day 14 after administration the patient developed lower legs weakness and myalgia. The result of examination of animal force of limbs wasⅣlevel. The serum potassium was 1. 1 mmol/L. Compound glycyrrhizin and benazepril were stopped. The patient was given potassium chloride tablets 2. 0 g orally and an IV infusion of 10% potassium chloride 40 ml dissolved in more than 1 000 ml of 0. 9% sodium chloride. The speed of IV infusion was 1. 0 g potassium chloride/hour. The serum potassium was 1. 5 mmol/L four hours after drug withdrawal. The spirolactone 40 mg three times daily was added. Twenty four hours later,the patient′s serum potassium increased to 2. 9 mmol/L,his symptom of myasthenia of limbs improved. Forty eight hours later,his serum potassium was 3. 1 mmol/L,but he developed whole body myalgia,distending pain in both lower extremities and choking sensation in chest. Laboratory test showed creatine kinase( CK)50 940 U/L,CK-MB 441 U/L,lactate dehydrogenase( LDH) 1 992 U/L,aspartate aminotransferase( AST)441 U/L,T( cTnT)0. 20 ng/ml,serum creatinine( SCr) 317 μmol/L,blood urine nitrogen( BUN)18. 2 mmol/L,uric acid( UA)533 mol/L,and carban dioxide-combining Power(CO2-CP)35. 5 mmol/L. Myoglobins in blood and urine did not tested. The color of urine was dark brown. The patient was diagnosed severe hypokalemic,rhabdomyolysis,and renal injury. He received the symptomatic treatments of potassium supplement, fluid infusion, nutritional support, and protection of renal function. On day 5 of compound glycyrrhizin withdrawal,the laboratory test showed the following values:serum potassium 3. 3 mmol/L,CK 10 292 U/L,Cr 252 μmo/L,BUN 16. 3 mmol/L,UA 472 μmo/L;on day 10 of compound glycyrrhizin withdrawal,the laboratory test showed the following values:serum potassium 4. 6 mmol/L,CK 107 U/L,Cr 116 μmo/L,BUN 6. 4 mmol/L,UA 189 μmol/L. His blood pressure was maintained at 130-160/70-90 mmHg,and the symptoms of myasthenia of limbs,myalgia in whole body disappeared completely.

Objective To study the changes of plasma brain natriuretic peptide(BNP)level in patients with atrial fibrillation(AF),and explore the relationship between plasma level of BNP and paroxysmal AF,persistent AF,rheumatic AF and non-rheumatic AF.Methods In total,158 outpatients and inpatients diagnosed as heart diseases were selected from the Affiliated Hospital of Inner Mongolia Medical College during October 2005 to January 2007,83 with AF and 75 without AF.Their cardiac function Was evaluated according to the classification system of the New York Heart Association(NYHA)in the United States.Plasma level of BNP was measured with bi-monoclonal antibody microparticulate enzyme immunoassay(MEIA).Results Plasma BNP level was significantly higher in AF group[(112±75)ng/L]than that in non-AF group[(39±26)ng/L,P＜0.05]with same ventricular function.No significant difference in plasma level of BNP between the groups with paroxysmal AF[(113±84)ng/L]and with persistent AF[(110±69)ng/L]was found.Plasma level of BNP Was(84±73)ng/L in the group with rheumatic valvular AF,not significantly different from that in non-valvular AF[(117±76)ng/L,P＞0.05].Conclusion Plasma BNP level is higher in AF group than that in non-AF group,but no significant difference in plasma BNP level between patients with paroxysmal AF,persistent AF,rheumatic valvular AF and non-rheumatic AF,under same ventricular function,was found.

Objective To observe the influence of the short effect antihypertension drugs- nifedipine and medial effect antihypertension drugs- extended release nifedipine on the blood pressure variability (BPV) in essential hypertension(EH). Methods Twenty-five EH patients were underwent 24-hour noninvasive ambulatory blood pressure monitoring (ABPM) and observed their BPV respectively before taking drugs, after taking nifedipine and extended release nifedipine. Meantime,25 normotensive controls (NC) were observed. Results (1)BPV in EH group was higher than that in controlled group and the severer the rise of blood pressure, the more obvious the increase of BPV (P 0.05). Conclusions Nifedipine could increase BPV but extended release nifedipine did not change BPV while they decreased blood pressure. Effect of extended release nifedipine was better than nifedipine in decreasing blood pressure.

Objective To investigate the associations between the left ventricular geometry and the insulin resistance (IR) in elderly patients with essential hypertension (EH) and the effect of the IR on the left ventricular geometry. Methods According to left ventricular mass index (LVMI)and relative wall thickness(RWT),72 EH patients were divided into normal geometry group (34 cases ),concentric remodeling group (18 cases ),concentric hypertrophy group (11 cases ),and eccentric hypertrophy group (9 cases ).The oral glucose tolerance test and insulin release test were performed in all patients and 35 healthy subjects as control.The area under curve of glucose tolerance (AG), area under curve of insulin release (AI), fasting serum insulin /fasting serum glucose (FIS/FSG) ratio, AI/AG ratio and insulin sensitivity index (ISI) were calculated.Correlation between RWT,LVMI and 7 insulin-sensitivity parameters were respectively obtained using linear regression analysis,and the stepwise regression analysis was used to assess the independent effect of each parameter. Results RWT was positively correlated with AG、 AI(r=0.160、0.227, respectively, all P0.05). Only ISI was independently correlated with RWT (r2=0.071,P