1.Effect of individualized exercise intervention on weight loss and improvement of metabolic indexes in individuals with metabolic syndrome
Zhengfang WANG ; Fengxu ZHANG ; Xu ZOU ; Zhiying SUN ; Han ZHANG
Chinese Journal of Health Management 2024;18(5):361-365
Objective:To analyze the effect of individualized exercise intervention on weight loss and improvement of metabolic indexes in individuals with metabolic syndrome (MS).Methods:This study was a randomized controlled trial. A total of 1 200 MS patients who underwent health examinations in the Beijing Aerospace General Hospital Healthy Management Center from 2019 to 2022 were selected as the research subjects. The patients were randomly divided into the experimental group (600 cases) and the control group (600 cases) by random number table. Based on the patient′s physical fitness data, a 3-month personalized exercise intervention was implemented for the experimental group, which included aerobic exercise 3 to 4 times/week combined with resistance exercise≥2 times/week, and MS-related health examinations were given too. The control group only received physical examination. Paired t-test was used to compare the changes in weight and metabolic-related indicators before and after the intervention in the two groups. Two-sample t-test was used to compare the differences in intervention effects between groups. The effect of personalized exercise intervention on weight loss and improvement of metabolic indicators in the MS population was analyzed. Results:After the intervention, the weight, body mass index, waist-to-hip ratio, resting heart rate, systolic blood pressure, diastolic blood pressure, fasting glucose, low-density lipoprotein, total cholesterol esters, and triglyceride levels in the experimental group were all significantly lower than those before [(72.5±12.9) vs (74.2±13.6) kg, (27.3±3.5) vs (27.9±3.5) kg/m2, 0.87±0.08 vs 0.91±0.08, (71±7) vs (74±9) times/min, (131±11) vs (138±14) mmHg (1 mmHg=0.133 kPa), (80±8) vs (85±9) mmHg, (6.0±1.1) vs (6.9±1.6) mmol/L, (2.78±0.78) vs (3.12±0.77) mmol/L, (4.62±1.04) vs (5.22±0.97) mmol/L, (1.36±0.42) vs (2.59±2.01) mmol/L], but the high-density lipoprotein level was significantly higher than that before [(1.31±0.31) vs (1.27±0.29) mmol/L] (all P<0.05). The weight, body mass index, waist-to-hip ratio, systolic blood pressure, low-density lipoprotein, total cholesterol esters, and triglyceride levels after the intervention in the control group were all significantly higher than those before [(68.1±5.9) vs (67.1±5.9) kg, (25.3±2.4) vs (24.9±2.4) kg/m2, 0.83±0.07 vs 0.82±0.06, (127±12) vs (125±12) mmHg, (3.50±1.45) vs (3.20±1.21) mmol/L, (5.50±1.80) vs (5.30±1.52) mmol/L, (1.59±0.82) vs (1.40±0.65) mmol/L], but the high-density lipoprotein level was significantly lower than that before the intervention [(1.28±0.28) vs (1.38±0.28) mmol/L] (all P<0.05). The intervention effects on weight, body mass index, waist-to-hip ratio, heart rate, systolic blood pressure, diastolic blood pressure, glucose, high-density lipoprotein, low-density lipoprotein, total cholesterol and triglycerides levels in the experimental group were all significantly better than those in the control group [(-1.4±13.3) vs (1.0±5.9) kg, (-0.6±3.5) vs (0.4±2.4) kg/m2, -0.04±0.08 vs 0.01±0.06, (-3±8) vs (0±7) times/min, (-7±12) vs (2±12) mmHg, (-5±9) vs (0±8) mmHg, (-0.9±1.4) vs (0±0.5) mmol/L, (0.04±0.30) vs (-0.10±0.28) mmol/L, (-0.34±0.77) vs (0.30±1.34) mmol/L, (-0.60±1.00) vs (0.20±1.66) mmol/L, (-1.23±1.45) vs (0.19±0.74) mmol/L ] (all P<0.001). Conclusion:Individualized exercise intervention can effectively promote weight loss and improve metabolic-related indicators in MS patients.
2.Analysis on the correlation between changes of waist circumference and blood lipids in military pilots and intervention suggestions
Zhiying LUO ; Jun LIU ; Weifang WANG ; Chao CHENG ; Jie ZOU ; Yanchao TANG ; Yanqiu GONG ; He ZHANG
Chinese Journal of Aerospace Medicine 2024;35(3):187-192
Objective:To provide basis for personalized health interventions for military pilots by analyzing the longitudinal variation trend of waist circumference and its correlation with BMI and lipid metabolism indicators among military pilots.Methods:A retrospective analysis was used on the base of collecting the physical examination data of 323 pilots who had been consecutively recuperated for 3 times and completed 3 health indicator examinations in Air Force Healthcare Center for Special Services Hangzhou from January of 2017 to January of 2022. The pilots were divided into waist circumference <85 cm group, ≥85-90 cm group, and ≥90 cm group. According to their waist circumference change trend, they were divided into increased group (difference of waist circumference >0 cm) and decreased group (difference of waist circumference ≤0 cm). According to the 5-year change rate of waist circumference (Δ5), they were divided into waist circumference relative increased group (Δ5>3%), relative stable group (-3%≤Δ5≤3%), and relative decreased group (Δ5<-3%). The stratification of different waist circumference, the 5-year change rate of waist circumference and BMI, blood lipid indexes and abnormal rate of hyperlipidemia were compared. Waist circumference was measured and determined according to the relevant standards for flying personnel and adults.Results:①The mean waist circumference of 323 pilots was close to the value of pre-central obesity. The mean BMI had reached the overweight standard, and the mean TG and TC were at high end of normal values.②During the 5-year period, the values of blood lipid metabolism indicators were repeatedly measured 3 times, and the mean values of TG, TC, and LDL-C showed an increasing trend, with significant differences ( F=7.39, 9.49, 3.67, P=0.001,<0.001, =0.026) and the mean values of TG and TC of the third measurement had approached to high limit. ③The results of BMI, TG in 3 measurements, LDL-C in the first and third measurements, TC in the first measurement and the incidence of hyperlipidemia were all increased with the increase of waist circumference and the differences were significant ( F=3.06-133.39, χ2=9.08-22.99, all P<0.05); HDL-C decreased with the increase of waist circumference, with significant differences ( F=6.32, 9.78, 10.86, P=0.002, <0.001, <0.001). The mean BMI of the ≥85-90 cm group reached the overweight level in the 3 measurements. The mean values of TG and TC in the first 2 measurements were at high end of normal value, but the mean values in the third measurement were higher than the normal range. The mean values of BMI, TG, and TC in ≥90 cm group were all within the abnormal range. ④ The 5-year change rate of BMI and LDL-C in the waist circumference increased group were higher than those in the waist circumference decreased group ( F=26.02, 14.95, both P<0.001). ⑤The waist circumference relative stable and relative increased group had a higher incidence of new hyperlipidemia, with 27 cases (21.77%) and 24 cases (20.00%) respectively. The waist circumference relative decreased group had the highest improvement rate of hyperlipidemia in 5 cases (6.33%), but the difference was not significant (all P>0.05). Conclusions:The abnormal rates of waist circumference, BMI, and blood lipids in military pilots are relatively high and show an increasing trend. The changes in waist circumference are closely related to BMI and blood lipids, and maintaining waist circumference within a normal range helps to maintain stability of blood lipid metabolism.
3.Preliminary investigation on the risk of ischemic cardiovascular disease in special service personnel and its correlation with behavior and lifestyle
Zhiying LUO ; Jie ZOU ; He ZHANG ; Jun LIU ; Xiaoxia JIANG ; Weifang WANG
Journal of Navy Medicine 2024;45(8):800-805
Objective To study the correlation between the risk of ischemic cardiovascular disease(ICVD)and behavior and life style of special service personnel,and to provide targeted reference for health intervention of special service personnel,so as to reduce the incidence of ICVD and ensure the combat effectiveness.Methods A total of 1 205 special service personnel in a sanatorium in Hangzhou from January 2019 to December 2021 were enrolled in this retrospective study.Their health records were collected and sorted out.A self-made health behavior and lifestyle questionnaire survey was conducted among them under the guidance of professionals.Results There were significant differences in the prevalence of smoking,and the incidences of diabetes and hypercholesterolemia among different age groups(χ2=13.481,P=0.009;χ2=13.888,P=0.008;χ2=24.505,P<0.001).There were no significant differences in the proportion of people with BMI≥24 or systolic blood pressure≥140 mmHg among different age groups(χ2=9.247,P=0.055;χ2=2.400,P=0.663).The mean absolute risk of ICVD in the next 10 years was(1.44±1.30)%.In the next 10 years,there was 1 case(0.08%)with a medium absolute risk of ICVD,37 cases(3.07%)with low risk,and 1 167 cases(96.85%)with extremely low risk.The relative risk of ICVD significantly increased with age in the next 10 years(χ2=25.774,P<0.001).There were significant differences between the relative risk of ICVD and behavior and lifestyle among the special service personnel in the next 10 years in terms of average daily intake of fish and aquatic products,average daily intake of egg and egg products,breakfast days per week,average weekly exercise time,average weekly passive smoking,and years of alcohol consumption(F=9.794,P=0.002;F=7.523,P=0.006;F=5.622,P=0.018;F=5.955,P=0.015;F=4.777,P=0.029;F=18.946,P<0.001).The average daily intake of fish and aquatic products and years of alcohol consumption were risk factors for ICVD(OR=1.330,P=0.004;OR=1.482,P<0.001).Breakfast days per week was protective factor for ICVD(OR=0.772,P=0.024).Conclusion The development of healthy behavior and lifestyle of special service personnel is of great significance for the prevention of ICVD.With the increase of age,the risk of ICVD is relatively increased.Developing good living and eating habits,eating breakfast every day,reasonable diet,smoking cessation and alcohol restriction have a certain protective effect on the prevention of ICVD.
4.Analysis on the correlation between changes of waist circumference and blood lipids in military pilots and intervention suggestions
Zhiying LUO ; Jun LIU ; Weifang WANG ; Chao CHENG ; Jie ZOU ; Yanchao TANG ; Yanqiu GONG ; He ZHANG
Chinese Journal of Aerospace Medicine 2024;35(3):187-192
Objective:To provide basis for personalized health interventions for military pilots by analyzing the longitudinal variation trend of waist circumference and its correlation with BMI and lipid metabolism indicators among military pilots.Methods:A retrospective analysis was used on the base of collecting the physical examination data of 323 pilots who had been consecutively recuperated for 3 times and completed 3 health indicator examinations in Air Force Healthcare Center for Special Services Hangzhou from January of 2017 to January of 2022. The pilots were divided into waist circumference <85 cm group, ≥85-90 cm group, and ≥90 cm group. According to their waist circumference change trend, they were divided into increased group (difference of waist circumference >0 cm) and decreased group (difference of waist circumference ≤0 cm). According to the 5-year change rate of waist circumference (Δ5), they were divided into waist circumference relative increased group (Δ5>3%), relative stable group (-3%≤Δ5≤3%), and relative decreased group (Δ5<-3%). The stratification of different waist circumference, the 5-year change rate of waist circumference and BMI, blood lipid indexes and abnormal rate of hyperlipidemia were compared. Waist circumference was measured and determined according to the relevant standards for flying personnel and adults.Results:①The mean waist circumference of 323 pilots was close to the value of pre-central obesity. The mean BMI had reached the overweight standard, and the mean TG and TC were at high end of normal values.②During the 5-year period, the values of blood lipid metabolism indicators were repeatedly measured 3 times, and the mean values of TG, TC, and LDL-C showed an increasing trend, with significant differences ( F=7.39, 9.49, 3.67, P=0.001,<0.001, =0.026) and the mean values of TG and TC of the third measurement had approached to high limit. ③The results of BMI, TG in 3 measurements, LDL-C in the first and third measurements, TC in the first measurement and the incidence of hyperlipidemia were all increased with the increase of waist circumference and the differences were significant ( F=3.06-133.39, χ2=9.08-22.99, all P<0.05); HDL-C decreased with the increase of waist circumference, with significant differences ( F=6.32, 9.78, 10.86, P=0.002, <0.001, <0.001). The mean BMI of the ≥85-90 cm group reached the overweight level in the 3 measurements. The mean values of TG and TC in the first 2 measurements were at high end of normal value, but the mean values in the third measurement were higher than the normal range. The mean values of BMI, TG, and TC in ≥90 cm group were all within the abnormal range. ④ The 5-year change rate of BMI and LDL-C in the waist circumference increased group were higher than those in the waist circumference decreased group ( F=26.02, 14.95, both P<0.001). ⑤The waist circumference relative stable and relative increased group had a higher incidence of new hyperlipidemia, with 27 cases (21.77%) and 24 cases (20.00%) respectively. The waist circumference relative decreased group had the highest improvement rate of hyperlipidemia in 5 cases (6.33%), but the difference was not significant (all P>0.05). Conclusions:The abnormal rates of waist circumference, BMI, and blood lipids in military pilots are relatively high and show an increasing trend. The changes in waist circumference are closely related to BMI and blood lipids, and maintaining waist circumference within a normal range helps to maintain stability of blood lipid metabolism.
5.Preliminary investigation on the risk of ischemic cardiovascular diseases in the military pilots examined in a sanatorium
Jie ZOU ; Chao CHENG ; Youqin ZHANG ; Xiangdong CHEN ; Jun LIU ; Weifang WANG ; He ZHANG ; Zhiying LUO
Chinese Journal of Aerospace Medicine 2022;33(4):199-204
Objective:To provide references for cardiovascular health management of military pilots by investigating the risk of ischemic cardiovascular diseases (ICVD) in military pilots.Methods:The physical examination data of military pilots in Air Force Healthcare Center for Special Services Hangzhou were retrospectively analyzed. The military pilots were divided into 2 groups by age (22-34 years, 35-56 years), and they were divided into 5 groups by flying hours (≤500 h, 500-≤1 000 h, 1 000-≤2 000 h, 2 000-≤3 000 h, >3 000 h). The 10-year ICVD incidence risk of pilots was evaluated according to the 10-year ICVD incidence risk assessment table of Chinese people. The distribution and influencing factors of absolute risk and relative risk of ICVD incidence in military pilots were analyzed, and the risk factors between different age groups were compared.Results:①A total of 337 military pilots were included, 194 in the 22-34 years group and 143 in the 35-56 years group. ②Absolute risk detection: there were 336 very low-risk military pilots and a low-risk military pilot. Relative risk detection: 87 (25.82%) military pilots were relatively high risk including 41 (21.13%) cases in 22-34 years group, 46 (32.17%) cases in 35-56 years group, the difference between 2 groups was statistically significant ( χ2=5.23, P=0.022); there were 250 (74.18%) relatively low-risk military pilots. ③Among the pilots aged 22-34 years, there was significant difference in relative high-risk ratio between flying hours ( χ2=17.00, P<0.001). The relative high-risk ratio of the pilots with flying hours 500-≤1 000 h was higher than that of pilots with flying hours ≤500 h, and the difference was statistically significant ( P<0.05); the proportion of pilots with elevated triglyceride and low density lipoprotein cholesterol indexes was higher than that of those with normal indicators (all P<0.05). The relative high-risk ratio of pilots with hypertension in 35-56 years group was higher than that of those with normal blood pressure ( χ2=23.70, P<0.001); the proportion of pilots with elevated triglyceride indicators was higher than that of high-risk groups ( P<0.05). ④The smoking rate, BMI and total cholesterol abnormality rate were higher in the 35-56 years group than in the 22-34 years group, and the differences were statistically significant ( χ2=9.71, 29.72, 19.17, P=0.002, <0.001,<0.001). ⑤The aggregation analysis of the risk factors of smoking, BMI, total cholesterol, systolic blood pressure and diabetes showed that the number of risk factor aggregates ≥2 species accounted for 51.04%, and the aggregation of risk factors in different age groups was statistically significant ( Z=6.38, P<0.001). Conclusions:The relative risk of ICVD in pilots is high, and the aggregation of risk factors is serious. Simply improving a single index is hardly to meet the demand of risk reduction. It needs a variety of joint interventions to improve the lifestyle and reduce the abnormal rate of total cholesterol, overweight rate, systolic blood pressure level and smoking rate. It is suggested that ICVD incidence risk prediction score should be routinely used in the risk assessment of chronic diseases in aviation medicine.
6.Preliminary investigation on the risk of ischemic cardiovascular diseases in the military pilots examined in a sanatorium
Jie ZOU ; Chao CHENG ; Youqin ZHANG ; Xiangdong CHEN ; Jun LIU ; Weifang WANG ; He ZHANG ; Zhiying LUO
Chinese Journal of Aerospace Medicine 2022;33(4):199-204
Objective:To provide references for cardiovascular health management of military pilots by investigating the risk of ischemic cardiovascular diseases (ICVD) in military pilots.Methods:The physical examination data of military pilots in Air Force Healthcare Center for Special Services Hangzhou were retrospectively analyzed. The military pilots were divided into 2 groups by age (22-34 years, 35-56 years), and they were divided into 5 groups by flying hours (≤500 h, 500-≤1 000 h, 1 000-≤2 000 h, 2 000-≤3 000 h, >3 000 h). The 10-year ICVD incidence risk of pilots was evaluated according to the 10-year ICVD incidence risk assessment table of Chinese people. The distribution and influencing factors of absolute risk and relative risk of ICVD incidence in military pilots were analyzed, and the risk factors between different age groups were compared.Results:①A total of 337 military pilots were included, 194 in the 22-34 years group and 143 in the 35-56 years group. ②Absolute risk detection: there were 336 very low-risk military pilots and a low-risk military pilot. Relative risk detection: 87 (25.82%) military pilots were relatively high risk including 41 (21.13%) cases in 22-34 years group, 46 (32.17%) cases in 35-56 years group, the difference between 2 groups was statistically significant ( χ2=5.23, P=0.022); there were 250 (74.18%) relatively low-risk military pilots. ③Among the pilots aged 22-34 years, there was significant difference in relative high-risk ratio between flying hours ( χ2=17.00, P<0.001). The relative high-risk ratio of the pilots with flying hours 500-≤1 000 h was higher than that of pilots with flying hours ≤500 h, and the difference was statistically significant ( P<0.05); the proportion of pilots with elevated triglyceride and low density lipoprotein cholesterol indexes was higher than that of those with normal indicators (all P<0.05). The relative high-risk ratio of pilots with hypertension in 35-56 years group was higher than that of those with normal blood pressure ( χ2=23.70, P<0.001); the proportion of pilots with elevated triglyceride indicators was higher than that of high-risk groups ( P<0.05). ④The smoking rate, BMI and total cholesterol abnormality rate were higher in the 35-56 years group than in the 22-34 years group, and the differences were statistically significant ( χ2=9.71, 29.72, 19.17, P=0.002, <0.001,<0.001). ⑤The aggregation analysis of the risk factors of smoking, BMI, total cholesterol, systolic blood pressure and diabetes showed that the number of risk factor aggregates ≥2 species accounted for 51.04%, and the aggregation of risk factors in different age groups was statistically significant ( Z=6.38, P<0.001). Conclusions:The relative risk of ICVD in pilots is high, and the aggregation of risk factors is serious. Simply improving a single index is hardly to meet the demand of risk reduction. It needs a variety of joint interventions to improve the lifestyle and reduce the abnormal rate of total cholesterol, overweight rate, systolic blood pressure level and smoking rate. It is suggested that ICVD incidence risk prediction score should be routinely used in the risk assessment of chronic diseases in aviation medicine.
7.The phenotypic and genetic spectrum of colony-stimulating factor 1 receptor gene-related leukoencephalopathy in China
Jingying WU ; Zaiqiang ZHANG ; Qing LIU ; Jun XU ; Weihai XU ; Liyong WU ; Zhiying WU ; Kang WANG ; Jianjun WU ; Zhangyu ZOU ; Haishan JIANG ; Wei ZHANG ; Wei GE ; Yuhu ZHANG ; Tongxia ZHANG ; Lixia ZHANG ; Zhanhang WANG ; Li LING ; Chang ZHOU ; Yun LI ; Beisha TANG ; Jianguang TANG ; Ping ZHONG ; Liang SHANG ; Yimin SUN ; Guixian ZHAO ; Xiuhe ZHAO ; Hongfu LI ; Jiong HU ; Jieling JIANG ; Chao ZHANG ; Xinghua LUAN ; Yuwu ZHAO ; Wotu TIAN ; Feixia ZHAN ; Xiaohang QIAN ; Huidong TANG ; Yuyan TAN ; Chunkang CHANG ; Youshan ZHAO ; Li CAO
Chinese Journal of Neurology 2021;54(11):1109-1118
Objective:To summarize and analyze the clinical data of Chinese patients with colony-stimulating factor 1 receptor (CSF1R)-related leukoencephalopathy, and clarify the phenotypic and genetic characteristics of Chinese patients.Methods:Medical history of patients with CSF1R-related leukoencephalopathy diagnosed from April 1, 2018 to January 31, 2021 in the department of neurology of 22 hospitals in China was collected, and scores of Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment Scale (MoCA), magnetic resonance severity scale were evaluated. Group comparison was performed between male and female patients.Results:A total of 62 patients were included, and the male-female ratio was 1∶1.95. The age of onset was (40.35±8.42) years. Cognitive impairment (82.3%, 51/62) and motor symptoms (77.4%,48/62) were the most common symptoms. The MMSE and MoCA scores were 18.79±7.16 and 13.96±7.23, respectively, and the scores of two scales in male patients (22.06±5.31 and 18.08±5.60) were significantly higher than those in females (15.53±7.41 , t=2.954, P=0.006; 10.15±6.26, t=3.328 , P=0.003). The most common radiographic feature was bilateral asymmetric white matter changes (100.0%), and the magnetic resonance imaging severity scale score was 27.42±11.40, while the white matter lesion score of females (22.94±8.39) was significantly higher than that of males (17.62±8.74 , t=-2.221, P<0.05). A total of 36 CSF1R gene mutations were found in this study, among which c.2381T>C/p.I794T was the hotspot mutation that carried by 17.9% (10/56) of the probands. Conclusions:The core phenotypic characteristics of CSF1R-related leukoencephalopathy in China are progressive motor and cognitive impairment, with bilateral asymmetrical white matter changes. In addition, there exist gender differences clinically, with severer cognitive impairment and imaging changes in female patients. Thirty-six CSF1R gene mutations were found in this study, and c.2381T>C/p. I794T was the hotspot mutation.
8.A multicenter retrospective study on the etiology of necrotizing pneumonia in children
Yunlian ZHOU ; Jinrong LIU ; Qiuwei YI ; Lina CHEN ; Zhiying HAN ; Changdi XU ; Suyan LIU ; Chuangli HAO ; Jing LIU ; Qiaoling LI ; Lijun WANG ; Chao WANG ; Guanghua CHE ; Yuanyuan ZHANG ; Lin TONG ; Yeqing LIU ; Shunying ZHAO ; Yuejie ZHENG ; Shu LI ; Hanmin LIU ; Jie CHANG ; Deyu ZHAO ; Yingxue ZOU ; Xinxing ZHANG ; Guangmin NONG ; Hailin ZHANG ; Jianli PAN ; Yanni CHEN ; Xiaoyan DONG ; Yunfeng ZHANG ; Yingshuo WANG ; Dehua YANG ; Quan LU ; Zhimin CHEN
Chinese Journal of Pediatrics 2021;59(8):658-664
Objective:To investigate the etiology of necrotizing pneumonia (NP) in children and the clinical characteristics of NP caused by different pathogens in China.Methods:A retrospective, case-control study was performed in children with NP who were admitted to 13 hospitals in China from January 2008 to December 2019. The demographic and clinical information, laboratory data, etiological and radiological findings were analyzed. The data were divided into three groups based on the following years: 2008-2011, 2012-2015 and 2016-2019, and the distribution characteristics of the pathogens in different period were compared. Meanwhile, the pathogens of pediatric NP in the southern and northern China were compared. And the clinical characteristics of the Mycoplasma pneumoniae (MP) NP and the bacterial NP were also compared. T-test or Mann-Whitney nonparametric test was used for comparison of numerical variables, and χ 2 test was used for categorical variables. Results:A total of 494 children with NP were enrolled, the median ages were 4.7 (0.1-15.3) years, including 272 boys and 222 girls. Among these patients, pathogens were identified in 347 cases and the pathogen was unclear in the remaining 147 cases. The main pathogens were MP (238 cases), Streptococcus pneumoniae (SP) (61 cases), Staphylococcus aureus (SA) (51 cases), Pseudomonas aeruginosa (13 cases), Haemophilus influenzae (10 cases), adenovirus (10 cases), and influenza virus A (7 cases), respectively. MP was the most common pathogen in all three periods and the proportion increased yearly. The proportion of MP in 2016-2019 was significantly higher than that in 2012-2015 (52.1% (197/378) vs. 36.8% (32/87), χ 2= 6.654, P=0.010), while there was no significant difference in the proportion of MP in 2012-2015 and that in 2008-2011 (36.8% (32/87) vs. 31.0% (9/29), χ2=0.314, P=0.575).Regarding the regional distribution, 342 cases were in the southern China and 152 in the northern China. Also, MP was the most common pathogen in both regions, but the proportion of MP was higher and the proportion of SP was lower in the north than those in the south (60.5% (92/152) vs. 42.7% (146/342), χ 2=13.409, P<0.010; 7.9% (12/152) vs. 14.3% (49/342), χ 2= 4.023, P=0.045). Comparing the clinical characteristics of different pathogens, we found that fever and cough were the common symptoms in both single MP and single bacterial groups, but chest pain was more common (17.0% (34/200) vs. 6.1% (6/98), χ 2=6.697, P=0.010) while shortness of breath and wheezing were less common in MP group (16.0% (32/200) vs. 60.2% (59/98), χ 2=60.688, P<0.01; 4.5% (9/200) vs. 21.4% (21/98), χ 2=20.819, P<0.01, respectively). The white blood cell count, C-reactive protein and procalcitonin in the bacterial group were significantly higher than those in the MP group (14.7 (1.0-67.1)×10 9/L vs. 10.5 (2.5-32.2)×10 9/L, 122.5 (0.5-277.3) mg/L vs. 51.4 (0.5-200.0) g/L, 2.13 (0.05-100.00) μg/L vs. 0.24 (0.01-18.85) μg/L, Z=-3.719, -5.901 and -7.765, all P<0.01). Conclusions:The prevalence of pediatric NP in China shows an increasing trend during the past years. MP, SP and SA are the main pathogens of NP, and the most common clinical symptoms are fever and cough. The WBC count, C-reactive protein and procalcitonin in bacterial NP are significantly higher than those caused by MP.
9.MicroRNA differential expression in liver cirrhosis rats undergoing human umbilical cord mesenchymal stem cell transplantation
Xiangzhong LIU ; Zhiqiang ZOU ; Guiqiang WANG ; Dong LI ; Zhiying SHAO
Chinese Journal of Tissue Engineering Research 2015;(23):3674-3680
BACKGROUND:Human umbilical cord mesenchymal stem cels (hUC-MSCs) can obviously relieve liver cirrhosis, and thereby repair liver injury. However, the molecular mechanism of hUC-MSCs therapy for liver cirrhosis is limited at present, and especialy the non-coding RNA regulation of hepatic gene changes has not been detailed. OBJECTIVE:To investigate the changes of microRNA after hUC-MSCs therapy in rats with liver cirrhosis. METHODS:Liver cirrhosis models were established in rats using carbon tetrachloride subcutaneous injection plus oral administration of alcohol. At 8 weeks after modeling, hUC-MSCs were injectedvia the tail vein once a week for 4 consecutive weeks. At 1 week after the last injection, rat liver tissues were colected for paraffin embedding. Liver RNA was extracted for gene chip analysis. Blood samples were colected and analyzed using an automatic biochemical analyzer to detect the changes of liver function. RESULTS AND CONCLUSION:Alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase were improved significantly after hUC-MSCs therapy. Fat lesions and necrosis of hepatocytes were significantly reduced. MicroRNA expression microarray hybridization analysis and PCR results showed that rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153* were down-regulated during modeling and increased after hUC-MSCs therapy. And rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a were firstly up-regulated in the process of modeling and then down-regulated obviously after hUC-MSCs therapy. These results suggest that hUC-MSCs may reverse liver cirrhosis and liver cel damage through up-regulation of rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153*, and down-regulation of rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a.
10.The Investigation of the Body Donation Will and Influence Factors of People in Yunnan Province
Wendong LUO ; Wenzhi JIN ; Hua ZOU ; Zhiying WAN ; Meng LI ; Jinde WANG ; Yanping DUAN
Chinese Medical Ethics 2015;(1):36-38
Objective:To investigate the mainstream view and statusof people on body donation in Yunnan province, and provide references. Methods:Random sampling questionnaire survey was used, the obtained data was statistically analyzed. Results:Only 48. 0% of the people understand the body donation, 20. 6% of people know body donation ways and procedures, 27. 8% of people have heard of body receiving station, 11. 4% of people know the body receiving station in yunnan province, 17. 2% of the people to understand body donation of relevant laws and regulations. 47. 4% of people that are willing to donate his body. Conclusion:Make perfect laws and reg-ulations, respect for the body, giving donors and their families allowance, body donation monument, to carry out education and publicity of the body donation form to the whole society support and participate in the good atmos-phere of body donation has great benefits.

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