Objective:To study the application value of vacuum drainage technology in radioactive wastewater discharge system of hospitals, and provide theoretical basis and reference for relevant institutes to design or renovate their radioactive wastewater discharge systems.Methods:Based on the composition of vacuum drainage system and gravity drainage system, the differences in water consumption and other factors of different systems were theoretically evaluated. To take the radioactive wastewater discharge system in a hospital as a research example, the storable periods of the fixed decay pool were calculated by using the two different drainage techniques.Results:Compared with the traditional gravity drainage technology, the vacuum drainage technology could save a lot of water and significantly reduce wastewater discharge. The water consumption was 16% of that of ordinary toilets with gravity drainage. By using the vacuum drainage technology to reform the radioactive wastewater discharge system of the hospital under the same amount of diagnosis and treatment workload, the storable period of radioactive wastewater could be extended by nearly three times, from 92.5 d before the renovation to 297 d after the renovation, without increasing the capacity of the decay pool. After the transformation, the total activity and volumetric activity concentration of 131I when discharging wastewater from each decay tank could be reduced to 4 096 Bq and 0.17 Bq/L, respectively, and the volumetric activity concentration was much lower than the national emission requirements. Conclusions:Vacuum drainage can significantly reduce wastewater production, and is worth promoting and applying in nuclear medicine departments.

Objective:To study the application value of vacuum drainage technology in radioactive wastewater discharge system of hospitals, and provide theoretical basis and reference for relevant institutes to design or renovate their radioactive wastewater discharge systems.Methods:Based on the composition of vacuum drainage system and gravity drainage system, the differences in water consumption and other factors of different systems were theoretically evaluated. To take the radioactive wastewater discharge system in a hospital as a research example, the storable periods of the fixed decay pool were calculated by using the two different drainage techniques.Results:Compared with the traditional gravity drainage technology, the vacuum drainage technology could save a lot of water and significantly reduce wastewater discharge. The water consumption was 16% of that of ordinary toilets with gravity drainage. By using the vacuum drainage technology to reform the radioactive wastewater discharge system of the hospital under the same amount of diagnosis and treatment workload, the storable period of radioactive wastewater could be extended by nearly three times, from 92.5 d before the renovation to 297 d after the renovation, without increasing the capacity of the decay pool. After the transformation, the total activity and volumetric activity concentration of 131I when discharging wastewater from each decay tank could be reduced to 4 096 Bq and 0.17 Bq/L, respectively, and the volumetric activity concentration was much lower than the national emission requirements. Conclusions:Vacuum drainage can significantly reduce wastewater production, and is worth promoting and applying in nuclear medicine departments.

Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including depression. Discs large homolog 1 (Dlg1), an adaptor protein, regulates cell polarization and the function of K

Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including depression. Discs large homolog 1 (Dlg1), an adaptor protein, regulates cell polarization and the function of K
Animals ; Depression/chemically induced* ; Inflammation ; Lipopolysaccharides/toxicity* ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microglia ; NF-kappa B ; Neuroinflammatory Diseases

The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.
Adjuvants, Immunologic ; Animals ; Antibodies, Neutralizing ; Antibody Formation ; Birds ; Bursa of Fabricius ; Cytokines ; Immunity, Cellular ; Immunity, Humoral ; Influenza A Virus, H9N2 Subtype ; Influenza in Birds ; Lung ; Mice ; Peptides ; T-Lymphocytes, Cytotoxic

BACKGROUNDAs a new member of inhibitor of apoptosis protein(IAP) family,Livin,especially Livin α,is known to be involved in occurrence and development of lung cancer.Livin is an important mechanism of chemotherapy resistance of lung cancer cell.The aim of this study is to set up Livin isoform(α & β)-specific gene silencing system in SPC-A1 cells by gene transfection and RNA interference(RNAi),and to explore the different functions and value of the isoforms in enhancing chemosensitivity of SPC-A1 cells.

METHODSLivinα+β,Livinα and Livinβ specific siRNA were expressed stably in SPC-A1 cells,respectively.MTT was performed to study sensitivity of the cells to chemotherapy drugs.In vivo experiment was performed to test sensitivity of mouse bearing tumor to cisplatin after gene silencing of Livin.

RESULTSAfter silencing of Livinα+β,Livinα and Livinβ genes,sensitivity of SPC-A1 cells to many chemotherapy drugs(including cisplatin,carboplatin,cyclophosphamide and adriblastine) was markedly increased(P < 0.05).Among them,gene silencing of Livinα+β showed the strongest enhancement effect on chemosensitivity of SPC-A1 cells(P < 0.01).Animal experiment showed that tumor inhibition rate of pSilencer-Livinα+β,pSilencer-Livinα and pSilencer-Livinβ groups was 146.1%,130.7% and 110.5%,respectively.

CONCLUSIONSThe results suggest that Livin isoform,especially Livinα+β is hopeful to be a molecular target for increasing sensitivity of lung cancer cell to chemotherapy.Gene silencing may be a new means of gene therapy for non-small cell lung cancer.

Object To study the glycosides from Swertia erythrosticta Maxim Methods The glycosides were isolated on silica gel column and purified by Sephadex LH20, their structures were identified by spectral data and chemical properties Results Seven compounds were obtained from aqueous extract and identified as swertianolin (Ⅰ), norswertianolin (Ⅱ), norswertiaglucoside (Ⅲ), isoorientin (Ⅳ), loganic acid (Ⅴ), gentiopicroside (Ⅵ) and ? gentiobiose (Ⅶ) Conclusion Compounds Ⅲ, Ⅳ, Ⅴ, and Ⅶ were first obtained from this plant

Object To study the chemical constituents from the whole plant of Swertia przewalskii Pissjauk Methods The constituents were isolated on silica gel column chromatography and their structures were identified by spectral and chemical analysis Results Fifteen compounds were isolated and identified as 1, 7 dihydroxyl 3, 8 dimethoxyxanthone (Ⅰ), 1, 8 dihydroxyl 3, 7 dimethoxyxanthone (Ⅱ), 1, 7, 8 trihydroxyl 3 methoxyxanthone (Ⅲ), 1, 3, 7, 8 tetrahydroxyxanthone (Ⅳ), 1 O ? D glucopyranosyl 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅴ), 1 O ? D glucopyranosyl 8 hydroxyl 3, 7 dime thoxyxanthone (Ⅵ), 7 O ? D glucopyranosyl 1, 8 dihydroxyl 3 methoxyxanthone (Ⅶ), 1 O D xylo pyranosyl (1→6) ? D glucopranosyl] 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 O D xylopyranos yl (1→6) ? D glucopytanosyl] 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 O D xylopyanosyl (1→6) ? D glucopyranosyl] 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( Ⅹ 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( ⅩⅡ ), oleanolic acid ( ⅩⅢ ), ursolic acid ( ⅩⅣ ) and getiopicroside ( ⅩⅤ ), respectively Conclusion Compounds Ⅳ～Ⅷ, Ⅺ, ⅩⅡ , ⅩⅣ and ⅩⅤ were obtained from this plant for the first time