Objective To evaluate the efficacy and safety of modified pelvic floor reconstruction in non-nerve-sparing robot-assisted radical prostatectomy (NNS RARP) for improving postoperative urinary control. Methods A retrospective analysis was conducted on the clinical data of 79 prostate cancer patients who underwent NNS RARP at Tangdu Hospital during Jan.2020 and Dec.2023, including 29 in the reconstruction group, and 50 in the non-reconstruction group. The baseline characteristics including age, body mass index, prostate-specific antigen (PSA) level, clinical stage, prostate volume, and biopsy Gleason score, and perioperative indexes including operation time, intraoperative blood loss, catheter indwelling time, complication rate, and positive rate of surgical margins were compared between the two groups. Additionally, urinary continence function was assessed before operation and 1,3,6, and 12 months after operation using the international consultation on incontinence questionnaire-short form (ICIQ-SF) and the incontinence quality of life questionnaire score (I-QoL). Results No statistically significant differences were observed in the baseline characteristics between the two groups (P>0.05). The operation time was significantly longer in the reconstruction group than in the non-reconstruction group [ (110.24±15.08) min vs. (101.80±9.89) min, P=0.010]. There were no significant differences in intraoperative blood loss, catheter indwelling time, complication rate, and positive rate of surgical margins between the two groups (P>0.05). The reconstruction group demonstrated significantly lower ICIQ-SF scores at 1 month [ (10.17±2.16) vs. (11.56±1.66), P=0.002],3 months [ (7.62±1.29) vs. (9.52±1.80), P<0.001], and 6 months postoperatively [ (4.93±1.22) vs. (6.18± 1.67), P=0.001]compared to the non-reconstruction group (adjusted P<0.0125). Conversely, the I-QoL scores were significantly higher in the reconstruction group at 1 month [ (73.32±10.30) vs. (63.88±9.55), P<0.001]and 3 months postoperatively [ (78.91±4.82) vs. (75.66±5.17), P=0.007] (adjusted P<0.0125). However, no significant differences were found in ICIQ-SF or I-QoL scores between the two groups preoperatively and 12 months postoperatively (adjusted P>0.0125). Conclusion The application of modified pelvic floor reconstruction technique in NNS RARP is safe and feasible. Although it slightly prolongs the operation time, it does not increase surgical risks; instead, it effectively promotes early recovery of postoperative urinary continence, thereby significantly enhancing patients'quality of life.

Objective:To investigate the effect and mechanism of esophageal squamous cell carcinoma exosomal miR-181b-5p to the polarization of M2 macrophages.Methods:Extracted and identified exosomes from esophageal squamous cell carcinoma,and detected the expression of miR-181b-5p in esophageal squamous cell carcinoma cells and their exosomes by qRT-PCR.M0 type macro-phages were divided into PBS group,HEEC exo group,Eca-109 exo group,miR-NC exo group,miR-181b-5p exo group,miR-NC group,miR-181b-5p mimic group,si-NC group,si-PTEN group,miR-181b-5p exo+PTEN group.qRT-PCR was used to detect the expressions of CD163,CD206,iNOS and TNF-α in each group.The targeting relationship between miR-181b-5p and PTEN were veri-fied by double luciferase reporter gene experiment.Results:miR-181b-5p was significantly overexpressed in esophageal squamous cell carcinoma cells TE-13,TE-12,TE-10,Eca-109,KYSE30 and their exosomes(P<0.001).Compared with miR-NC group,the expression of CD163 and CD206 in cells were significantly upregulated in the miR-181b-5p mimic group,as well as the expressions of iNOS and TNF-α were significantly downregulated(P<0.001).The results of double luciferase reporter genes showed that PTEN was the target gene of miR-181b-5p.Compared with si-NC group,the expressions of CD163 and CD206 in cells were significantly upregu-lated in the si-PTEN group,as well as the expressions of iNOS and TNF-α were significantly downregulated(P<0.001).Compared with PBS group,the expressions of CD163 and CD206 in cells were significantly upregulated in the Eca-109 exo group,as well as the expressions of iNOS and TNF-α were significantly downregulated(P<0.001).Compared with miR-NC exo group,the expressions of CD163 and CD206 in cells were significantly upregulated in the miR-181b-5p exo group,as well as the expressions of iNOS and TNF-α were significantly downregulated(P<0.001).Compared with miR-181b-5p exo group,the expressions of CD163 and CD206 in cells were significantly downregulated in the miR-181b-5p exo+PTEN group,as well as the expressions of iNOS and TNF-α were significantly upregulated(P<0.001).Conclusion:Exosomal miR-181b-5p inhibits PTEN expressions to promote M2 macrophage polarization.

Objective The main purpose of this study is to compare the embryo development and clinical outcomes of women in different age groups undergoing in vitro fertilization(IVF)processes using gonadotrophin-releasing hormone(GnRH)antagonist protocol,GnRH agonist long protocol,and early follicular phase protocol.We aim to provide reliable reference for future clinical treatments.Methods We conducted a detailed analysis of patients who underwent treatment between January 2021 and February 2023.1)In the overall patient population,we comprehensively compared the basic characteristics,the embryo development,and the clinical outcomes of patients treated with three different ovarian stimulation protocols,including the GnRH antagonist protocol group(n=4 173),the agonist long protocol group(n=2 410),and the early follicular phase long protocol group(n=341).2)We divided the overall population into three age groups,one group for patients under 30 years old(n=2 576),one for patients aged 30-35(n=3 249),and one for patients older than 35 years old(n=1 099).Then,we compared the three stimulation protocols based on the group division.We separately compared the embryo development and clinical outcomes of patients using the three stimulation protocols in the under 30 years old,the 30-35 years old,and the over 35 years old age groups.With this analysis,we aimed to explore the response of different age groups to different stimulation protocols and their impact on the success rate of IVF.Results 1)In the overall population,we found that the average number of oocytes retrieved in the GnRH agonist long protocol group was significantly higher than that in the GnRH antagonist protocol group([13.85±7.162]vs.[13.36±7.862],P=0.022 4),as well as the early follicular phase long protocol group([13.85±7.162]vs.[11.86±6.802],P<0.000 1).Patients in the GnRH antagonist protocol group not only had a significantly lower starting dose of gonadotrophin(Gn)compared to the other two groups(P<0.05)but also had a significantly lower number of days of Gn use(P<0.05).The blastocyst formation rate in the GnRH antagonist protocol group was the highest among the three groups,significantly higher compared to the GnRH agonist long protocol group(64.91% vs.62.35%,P<0.000 1)and the early follicular phase long protocol group(64.91% vs.61.18%,P=0.000 1).However,there were no significant differences in the clinical pregnancy rates or the live birth rates among the three groups treated with different ovarian stimulation protocols(P>0.05).2)In the<30 age group,the blastocyst formation rate in the GnRH antagonist protocol group was the highest among the three groups,significantly higher compared to the GnRH agonist long protocol group(66.12% vs.63.33%,P<0.000 1)and the early follicular phase long protocol group(66.12% vs.62.13%,P=0.009 4).In the 30-35 age group,the blastocyst formation rate in the GnRH antagonist protocol group was the highest among the three groups,significantly higher compared to the GnRH agonist long protocol group(64.88% vs.62.93%,P=0.000 9)and the early follicular phase long protocol group(64.88% vs.60.39%,P=0.001 1).In the>35 age group,the blastocyst formation rate in the GnRH antagonist protocol group was significantly higher than that in the GnRH agonist long protocol group(59.83% vs.56.51%,P=0.009 3),while there was no significant difference compared to that of the early follicular phase long protocol group(P>0.05).In the three age groups,we found that there were no significant differences in clinical pregnancy rate,live birth rate,and neonatal outcome indicators(fetal weight and Apgar score)among the three stimulation protocols(antagonist protocol,GnRH agonist long protocol,and early follicular phase long protocol)(P>0.05).The findings showed no significant differences between clinical and neonatal outcomes in patients of all ages,regardless of the ovarian stimulation protocol,suggesting that the three ovarian stimulation protocols have similar therapeutic effects in patients of different ages.The results of this study have important implications for the selection of an appropriate ovarian stimulation protocol and the prediction of treatment outcomes.Conclusion In the younger than 30 and 30-35 age groups,the GnRH antagonist protocol showed a more significant advantage over the GnRH agonist long protocol and the early follicular phase long protocol.This suggests that for younger and middle-aged patients,the antagonist protocol may lead to better outcomes during ovarian stimulation.In the older than 35 age group,while the antagonist protocol still outperformed the GnRH agonist long protocol,there was no significant difference compared to the early follicular phase long protocol.This may imply that with increasing age,the early follicular phase long protocol may have effects similar to the antagonist protocol to some extent.The advantages of the antagonist protocol lie in its ability to reduce stimulation duration and the dosage of GnRH,while enhancing patient compliance with treatment.This means that patients may find it easier to accept and adhere to this treatment protocol,thereby improving treatment success rates.Particularly for older patients,the use of the antagonist protocol may significantly increase the blastocyst formation rate,which is crucial for improving the success rates.Although there were no significant differences in the clinical outcomes of patients treated with the three protocols in each age group,further research is still needed to validate these findings.Future multicenter studies and increased sample sizes may help comprehensively assess the efficacy of different stimulation protocols.Additionally,prospective studies are needed to further validate these findings and determine the optimal treatment strategies.

The pursuit of cosmetic effects in post-surgical wounds has led to the development of ultra-minimally invasive techniques in surgery. Minimal invasive surgery has replaced open surgery and has become the new gold-standard for treating diseases. One such technique is the single incision triangulated umbilicus surgery (SITUS), which offers several advantages over traditional laparoscopic and other scarless surgeries, including reduced trauma, faster recovery, and better cosmetic outcomes. SITUS also has a short learning curve, aligns with conventional instrumentation operating habits, and can be used for whole abdominal surgeries. Chinese scholars have made further improvements to the SITUS technology, including expanding its applicability in intra-abdominal surgery and refining its incision closure methods to achieve superior cosmetic results. Currently, SITUS technology is experiencing rapid development in urology applications and has demonstrated satisfactory results in both domestic and international reports. This review aims to discuss the effectiveness and development of the SITUS technique in urology.

Objective:To investigate the embryo development and clinical pregnancy outcomes in patients with ovarian hyperstimulation syndrome (OHSS) undergoing assisted reproductive technology (ART).Methods:A retrospective cohort study was conducted on data from 4 080 cycles of in vitro fertilization/intracytoplasmic sperm injection-embryo transfer treatments performed at the Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University from August 2019 to August 2021. Patients were divided into OHSS group ( n=524) and non-OHSS group (control group, n=3 556) based on whether OHSS occurred, and the OHSS group was further divided into OHSS combined with polycystic ovary syndrome (PCOS) subgroup ( n=231) and OHSS combined with non-PCOS subgroup ( n=293) based on the presence of PCOS. General information, embryo developmental data and clinical outcomes were compared between the two groups. Results:1) Patients in the OHSS group [(30.7±3.6) years] were younger than those in control group [(31.5±4.8) years, P<0.001], and the number of retrieved oocytes (28.2±5.7), rates of high-quality embryos [52.7% (4 982/9 463)], blastocyst formation [54.0% (5 059/9 371)], biochemical pregnancy [75.0% (393/524)], clinical pregnancy [69.5% (364/524)], and live birth [58.0% (304/524)] were significantly higher in the OHSS group than in control group [12.5±6.7, 49.8% (14 042/28 204), 51.4% (14 279/27 797), 59.5% (2 115/3 556), 54.1% (1 924/3 556), 43.6% (1 550/3 556), respectively; all P<0.001]. 2) Patients in the OHSS combined with PCOS subgroup [(30.2±3.1) years] were younger than those in the OHSS combined with non-PCOS subgroup [(31.1±4.0) years, P=0.009], and the estradiol level [165.0 (101.0, 222.5) pmol/L] was higher than that in the OHSS combined with non-PCOS subgroup [141.0 (81.0,202.0) pmol/L, P=0.005]; rates of high-quality embryos [56.3% (2 413/4 284)], blastocyst formation [67.1% (2 846/4 239)], and high-quality blastocysts [57.7% (2 445/4 239)] were also significantly higher in the OHSS combined with PCOS subgroup than in the OHSS combined with non-PCOS subgroup [49.6% (2 569/5 179), 60.3% (3 092/5 132), 50.9% (2 614/5 132), respectively; all P<0.001]. Conclusion:There is a certain correlation between OHSS and female age. The occurrence of OHSS does not affect embryo development and does not increase adverse pregnancy outcomes in infertile patients. The presence of PCOS does not affect the pregnancy outcomes of OHSS patients. However, in ART, we still strive to avoid the occurrence of adverse events such as OHSS as much as possible.

Objective:To investigate the embryo development and clinical pregnancy outcomes in patients with ovarian hyperstimulation syndrome (OHSS) undergoing assisted reproductive technology (ART).Methods:A retrospective cohort study was conducted on data from 4 080 cycles of in vitro fertilization/intracytoplasmic sperm injection-embryo transfer treatments performed at the Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University from August 2019 to August 2021. Patients were divided into OHSS group ( n=524) and non-OHSS group (control group, n=3 556) based on whether OHSS occurred, and the OHSS group was further divided into OHSS combined with polycystic ovary syndrome (PCOS) subgroup ( n=231) and OHSS combined with non-PCOS subgroup ( n=293) based on the presence of PCOS. General information, embryo developmental data and clinical outcomes were compared between the two groups. Results:1) Patients in the OHSS group [(30.7±3.6) years] were younger than those in control group [(31.5±4.8) years, P<0.001], and the number of retrieved oocytes (28.2±5.7), rates of high-quality embryos [52.7% (4 982/9 463)], blastocyst formation [54.0% (5 059/9 371)], biochemical pregnancy [75.0% (393/524)], clinical pregnancy [69.5% (364/524)], and live birth [58.0% (304/524)] were significantly higher in the OHSS group than in control group [12.5±6.7, 49.8% (14 042/28 204), 51.4% (14 279/27 797), 59.5% (2 115/3 556), 54.1% (1 924/3 556), 43.6% (1 550/3 556), respectively; all P<0.001]. 2) Patients in the OHSS combined with PCOS subgroup [(30.2±3.1) years] were younger than those in the OHSS combined with non-PCOS subgroup [(31.1±4.0) years, P=0.009], and the estradiol level [165.0 (101.0, 222.5) pmol/L] was higher than that in the OHSS combined with non-PCOS subgroup [141.0 (81.0,202.0) pmol/L, P=0.005]; rates of high-quality embryos [56.3% (2 413/4 284)], blastocyst formation [67.1% (2 846/4 239)], and high-quality blastocysts [57.7% (2 445/4 239)] were also significantly higher in the OHSS combined with PCOS subgroup than in the OHSS combined with non-PCOS subgroup [49.6% (2 569/5 179), 60.3% (3 092/5 132), 50.9% (2 614/5 132), respectively; all P<0.001]. Conclusion:There is a certain correlation between OHSS and female age. The occurrence of OHSS does not affect embryo development and does not increase adverse pregnancy outcomes in infertile patients. The presence of PCOS does not affect the pregnancy outcomes of OHSS patients. However, in ART, we still strive to avoid the occurrence of adverse events such as OHSS as much as possible.

Objective To evaluate the application and effect of signature verification technology in children's vaccination clinics (CVC) of Jiangsu Province in 2020. Methods The signature verification data were derived from the Jiangsu Provincial Vaccination Integrated Service Management Information System, and the inquiry and registration, informed consent, vaccine traceability code scanning and observation information of children's vaccination clinics in different regions were analyzed. 210 doses of vaccination information were randomly selected from CVCs in each county, and the length of vaccination services in different regions was compared. Results During 2020, all of CVCs in Jiangsu were equipped with signature verification technology, and the signature verification rate of each vaccination sector was more than 99.90%. The length of outpatient vaccination service and overall length of stay in southern Jiangsu were slightly shorter than those in other regions. Conclusion The introduction of electronic signature verification technology in CVCs can effectively standardize the vaccination. It is necessary to expand the functions of electronic signature verification equipment, strengthen data analysis and utilization, and guide vaccination scientifically.

During cryopreservation of oocytes, the high toxicity and high permeability of cryoprotectants often result in the imbalance of oxidant-antioxidant system in oocytes especially the increase of intracellular reactive oxygen species (ROS) content. As an effective antioxidant and free radical scavenger, melatonin can significantly reduce the level of ROS in oocytes by affecting energy metabolism and signal transduction, and protect mitochondrial function. At the same time, it also plays the role of scavenging free radicals in oocytes, and realizes the suppression of intracellular oxidative stress levels during freezing-thawing process, thereby reducing the cryoinjury of oocytes during cryopreservation, ultimately, improving the blastocyst formation rate of frozen-thawed oocytes and the clinical pregnancy rate after embryo transfer. The application of melatonin in oocyte cryopreservation has a positive significance for the research of female fertility preservation. This article reviews the progress of melatonin in oocyte freezing.

During cryopreservation of oocytes, the high toxicity and high permeability of cryoprotectants often result in the imbalance of oxidant-antioxidant system in oocytes especially the increase of intracellular reactive oxygen species (ROS) content. As an effective antioxidant and free radical scavenger, melatonin can significantly reduce the level of ROS in oocytes by affecting energy metabolism and signal transduction, and protect mitochondrial function. At the same time, it also plays the role of scavenging free radicals in oocytes, and realizes the suppression of intracellular oxidative stress levels during freezing-thawing process, thereby reducing the cryoinjury of oocytes during cryopreservation, ultimately, improving the blastocyst formation rate of frozen-thawed oocytes and the clinical pregnancy rate after embryo transfer. The application of melatonin in oocyte cryopreservation has a positive significance for the research of female fertility preservation. This article reviews the progress of melatonin in oocyte freezing.

The etiology and pathogenesis of keloid are still not clear. It is believed that the formation of keloid is due to the joint action of many factors, during which process the external factor of fibroblast play an important role. Reprogramming of glucose metabolism in keloid fibroblasts is a new research direction. TGF-β 1 and its related TGF-β 1/Smad signalling pathway are considered to be the most closely related to the formation of keloid. This article reviews the research progress on extracellular factors, glucose metabolism and signaling pathways of keloids.