[Objective]To quantify lesion parameters in different retinal regions on ultra-widefield fluorescein angiography(UWFA)images of patients with diabetic retinopathy(DR)and to explore their association with center-involved diabetic macular edema(CI-DME).[Methods]We retrospectively analyzed UWFA and optical coherence tomography(OCT)images from 76 DR patients(101 eyes)initially diagnosed at Guangdong Provincial People's Hospital between June 2022 and June 2024.All images were captured on the same day.Eyes were classified into CI-DME and non-CI-DME groups based on central subfield thickness(CST)measured by OCT.Manual annotations of microaneurysms,non-perfusion areas,leakage areas,optic discs,and foveae were performed on UWFA images.Lesion parameters,including microaneurysm count,ischemic index,and leakage index,were quantified in the macular area,posterior area,mid-periphery,peripheral retina,and entire UWFA field.Differences in parameters between groups were compared,and their associations with CI-DME and diagnostic performance were assessed.[Results]The CI-DME group exhibited significantly higher microaneurysm count,ischemic index and leakage index in the macular area than those in the non-CI-DME eyes(all P<0.05).No significant differences were observed in these parameters in the peripheral retina between the two groups(all P＞0.05).ROC curve analysis showed that the leakage index could distinguish CI-DME better than the microaneurysm count and ischemia index.The macular leakage index had the highest discriminative ability,with an AUC of 0.80(95%CI:0.71,0.89).[Conclusion]This study found no significant association between peripheral lesion parameters on UWFA and CI-DME.However,macular lesion parameters,particularly the leakage index,were closely linked to CI-DME pathology.These findings highlight the importance of monitoring macular vascular stability to optimize clinical management for DME patients.

[Objective]To quantify lesion parameters in different retinal regions on ultra-widefield fluorescein angiography(UWFA)images of patients with diabetic retinopathy(DR)and to explore their association with center-involved diabetic macular edema(CI-DME).[Methods]We retrospectively analyzed UWFA and optical coherence tomography(OCT)images from 76 DR patients(101 eyes)initially diagnosed at Guangdong Provincial People's Hospital between June 2022 and June 2024.All images were captured on the same day.Eyes were classified into CI-DME and non-CI-DME groups based on central subfield thickness(CST)measured by OCT.Manual annotations of microaneurysms,non-perfusion areas,leakage areas,optic discs,and foveae were performed on UWFA images.Lesion parameters,including microaneurysm count,ischemic index,and leakage index,were quantified in the macular area,posterior area,mid-periphery,peripheral retina,and entire UWFA field.Differences in parameters between groups were compared,and their associations with CI-DME and diagnostic performance were assessed.[Results]The CI-DME group exhibited significantly higher microaneurysm count,ischemic index and leakage index in the macular area than those in the non-CI-DME eyes(all P<0.05).No significant differences were observed in these parameters in the peripheral retina between the two groups(all P＞0.05).ROC curve analysis showed that the leakage index could distinguish CI-DME better than the microaneurysm count and ischemia index.The macular leakage index had the highest discriminative ability,with an AUC of 0.80(95%CI:0.71,0.89).[Conclusion]This study found no significant association between peripheral lesion parameters on UWFA and CI-DME.However,macular lesion parameters,particularly the leakage index,were closely linked to CI-DME pathology.These findings highlight the importance of monitoring macular vascular stability to optimize clinical management for DME patients.

MTUS1 (microtubule-associated tumor suppressor 1) has been identified that can function as a tumor suppressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b (miR-19a/b) promote proliferation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell proliferation and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues. Third, MTUS1 was appraised as a direct target of miR-19a/b by bioinformatics analysis. Fourth, direct MTUS1 regulation by miR-19a/b in lung cancer cells was experimentally affirmed by cell transfection assay and luciferase reporter assay. Finally, miR-19a/b were shown to cooperatively repress MTUS1 expression and synergistically regulate MTUS1 expression to promote lung cancer cell proliferation and migration. In conclusion, our findings have provided the first clues regarding the roles of miR-19a/b, which appear to function as oncomirs in lung cancer by downregulating MTUS1.
A549 Cells ; Cell Movement ; Cell Proliferation ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Male ; MicroRNAs ; genetics ; metabolism ; RNA, Neoplasm ; genetics ; metabolism ; Tumor Suppressor Proteins ; biosynthesis ; genetics

OBJECTIVETo document the investigation and control of an outbreak of gastroenteritis in City G, South China, and provide a reference for preventing future outbreaks.

METHODSAn ambispective cohort study was designed. Attack rate (AR) and relative risks (RR) were calculated to identify the causes of gastroenteritis. Investigations using questionnaires included personal interviews with patients and doctors, reviews of medical records, laboratory examinations of fecal specimens and continuous hygiene monitoring of water samples from the waterworks.

RESULTSOverall, 427/71534 (AR=5.97%) cases were identified between October 31 and November 12 2010. Geographic distribution was highly localized, with 80% of cases occurring in the areas supplied by waterworks-A. Consumption of water provided solely by waterworks-A was found to be associated with illness (RR=8.20, 95 CI%:6.12-10.99) compared with that from waterworks-B. Microbiological analyses confirmed the presence of Norovirus in six of eight fecal samples from symptomatic patients, two water samples from waterworks-A and two sewage samples. After taking effective measures, the hygienic indices of waterworks-A met health criteria again on November 9 and no cases were reported 3 days later.

CONCLUSIONThe outbreak reported here was caused by drinking tap water contaminated with sewage at the source. Early identification of possible contamination sources and awareness of changes that might negatively impact water quality are important preventive measures to protect public health.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Disease Outbreaks ; Female ; Gastroenteritis ; epidemiology ; etiology ; Humans ; Infant ; Male ; Middle Aged ; Water Pollution ; adverse effects ; Young Adult

Objective To explore the best treatment for hepatitis B by observing the efficacy d interferon and adefovir dipivoxil (ADV) according to different combination of various quantification of HBV DNA and HBeAg. Methods One hundred and sixty-five hepatitis B antigen-positive patients were divided into four groups (A, B, C, D group) according to different quantification of HBV DNA and HBeAg. Each group was randomly divided into two sub-groups with interferon and the others with ADV. Items were observed in 48 weeks and statistic analysis was made to observe if there was significant difference between two methods in each group. Results There was no difference in the rate of HBV DNA inhibitory activity, HBV DNA clearance and ALT normalization as well as HBV DNA decreasing (level≥2lg copy/ml). But there was significant difference in the rate of HBeAg clearance, HBeAg seroconversion and HBeAg decreasing level ≥500.00 COL/ml (P<0.05). The efficacy decreased following the increasing of HBV DNA and HBeAg quantification, especially in ADV groups. The efficacy of interferon was better in seroconversion rate. Conclusion Quantification of HBV DNA and HBeAg can be the guidance to select different anti-virus methods.