BACKGROUND: Molecular subtyping is an essential complementarity after pathological analyses for targeted therapy. This study aimed to investigate the consistency of next-generation sequencing (NGS) results between circulating tumor DNA (ctDNA)-based and tissue-based in non-small cell lung cancer (NSCLC) and identify the patient characteristics that favor ctDNA testing. METHODS: Patients who diagnosed with NSCLC and received both ctDNA- and cancer tissue-based NGS before surgery or systemic treatment in Lung Cancer Center, Sichuan University West China Hospital between December 2017 and August 2022 were enrolled. A 425-cancer panel with a HiSeq 4000 NGS platform was used for NGS. The unweighted Cohen's kappa coefficient was employed to discriminate the high-concordance group from the low-concordance group with a cutoff value of 0.6. Six machine learning models were used to identify patient characteristics that relate to high concordance between ctDNA-based and tissue-based NGS. RESULTS: A total of 85 patients were enrolled, of which 22.4% (19/85) had stage III disease and 56.5% (48/85) had stage IV disease. Forty-four patients (51.8%) showed consistent gene mutation types between ctDNA-based and tissue-based NGS, while one patient (1.2%) tested negative in both approaches. Patients with advanced diseases and metastases to other organs would be suitable for the ctDNA-based NGS, and the generalized linear model showed that T stage, M stage, and tumor mutation burden were the critical discriminators to predict the consistency of results between ctDNA-based and tissue-based NGS. CONCLUSION ctDNA-based NGS showed comparable detection performance in the targeted gene mutations compared with tissue-based NGS, and it could be considered in advanced or metastatic NSCLC.
Humans ; Carcinoma, Non-Small-Cell Lung/pathology* ; Circulating Tumor DNA/blood* ; High-Throughput Nucleotide Sequencing/methods* ; Female ; Male ; Lung Neoplasms/pathology* ; Middle Aged ; Mutation/genetics* ; Aged ; Adult ; Aged, 80 and over

Objective By comprehensively applying the integrated technology acceptance model,we explore the accept-ability of"6S management work"among members within the medical group and analyze important influencing factors.This pro-vides theoretical support and practical guidance for promoting work progress and sustainable development.Methods In order to determine the specific items for the questionnaire,a combination of literature review and expert consultation methods was used.The entire hospital staff was stratified sampled proportionally,and an online survey was conducted to collect feedback on the ac-ceptability from 354 employees who had been employed and stably served in the hospital before 2024.The questionnaire results were statistically described using SPSS 27.0 to clarify the current satisfaction with management work and the relevant factors.Results The community influence was the highest in the independent variable with a score of(2.11±0.91),followed by per-formance expectation with a score of(2.11±0.75),and giving expectation scored the lowest with a score of only(1.99±0.46).The dependent variables of behavioural intention and satisfaction scored(1.96±0.75)and(1.97±0.85)respectively.Conclusion Using the key factors influencing the behavioural intentions and satisfaction of medical group members,such as co-operation,community influence,performance expectations,and payment expectations,recommendations are made for the contin-uous optimization of the"6S management"process.

Objective:To explore a new model of Total Quality Management in project management of National Natural Science Foundation of China (NSFC).Methods:The theory of Total Quality Management is applied to the management of NSFC funded project, that is, a complete and comprehensive process formed which covered the different stages of project cultivation, application, identification, implementation, completion and post-completion tracking; and the management concepts of whole process personnel engagement and comprehensive quality management are penetrated into those different stages, furthermore, corresponding key points should be focused are also analyzed.Results:The hospital has made great achievements in scientific research management, project quantity, outputs, and discipline construction.Conclusions:The introduction of Total Quality Management theory into NSFC management is conducive to continuously improve the level of fund management, and enhance the scientific and technological innovation capacity building of the hospital.

Antibody-mediated rejection (AMR) is a rare and serious complication after lung transplantation, with no characteristic of pathological manifestation, no systematic standard treatment, and the poor efficacy and prognosis. We reported a case of early AMR after lung transplantation and the relevant literature has been reviewed. A male patient presented with symptoms of cold 99 days after transplantation and resolved after symptomatic treatment. He admitted to the hospital 14 days later because of a sudden dyspnea and fever. Anti-bacteria, anti-fungi, anti-virus, and anti-pneumocystis carinii treatment were ineffective, and a dose of 1 000 mg methylprednisolone did not work too. The patient's condition deteriorated rapidly and tracheal intubation was done to maintain breathing. Serum panel reactive antibody and donor specific antibody showed postive in humen leukocyte antigen (HLA) II antibody. Pathological examination after transbronchial transplantation lung biopsy showed acute rejection. Clinical AMR was diagnosed combined the donor-specific antibody with the pathological result. The patient was functionally recovered after combined treatment with thymoglobuline, rituximab, plasmapheresis, and immunoglobulin. No chronic lung allograft dysfunction was found after 3 years follow up. We should alert the occurrence of AMR in lung transplantation recipient who admitted to hospital with a sudden dyspnea and fever while showed no effect after common anti-infection and anti-rejection treatment. Transbronchial transplantation lung biopsy and the presence of serum donor-specific antibody are helpful to the diagnosis. The treatment should be preemptive and a comprehensive approach should be adopted.
Graft Rejection ; Graft Survival ; HLA Antigens ; Humans ; Isoantibodies ; Lung Transplantation/adverse effects* ; Male

Objective To provide a system for warning, preventing and controlling emerging infectious diseases from a macroscopic perspective, using the COVID-19 epidemic data and effective distance model. Methods The dates of hospitalization/isolation treatment of the first confirmed cases of COVID-19 and the cumulative numbers of confirmed cases in different provinces in China reported as of 23 February, 2020 were collected. The Location Based Service (LBS) big data platform of 'Baidu Migration' was employed to obtain the data of the proportion of the floating population from Wuhan to all parts of the country. Effective distance models and linear regression models were established to analyze the relationship between the effective distance and the arrival time of the epidemic as well as the number of cumulative confirmed cases at provincial and municipal levels. Results The arrival time of the epidemic and the cumulative number of confirmed cases of COVID-19 had significant linear relationship at both provincial and municipal levels in China, and the regression coefficients of each linear model were significant ( P <0.001). At the provincial level, the effective distance could explain about 71% of the variation of the model with arrival time along with around 90% of the variation for the model in the cumulative confirmed case magnitude; at the municipal level, the effective distance could explain about 66% of the variation for the model in arrival time, and about 85% of the variation of the model with the cumulative confirmed case magnitude. Conclusions The fitting degree of the models are good. The LBS big data and effective distance model can be used to estimate the track, time and extent of epidemic spread to provide useful reference for early warning, prevention and control of emerging infectious diseases.

Capillary electrochromatography (CEC) is a micro-scale separation technique which is a hybrid between capillary electrophoresis (CE) and liquid chromatography (LC). CEC can be performed in packed, monolithic and open-tubular columns. In recent three years (from 2016 to 2018), enormous attention for CEC has been the development of novel stationary phases. This review mainly covers the development of novel stationary phases for open-tubular and monolithic columns. In particular, some biomaterials attracted increasing interest. There are no significant breakthroughs in technology and principles in CEC. The typical CEC applications, especially chiral separations are described.

To analyze the components of tumor infiltrating T lymphocyte (TIL) cells in malignant pleural effusion of lung adenocarcinoma, and evaluate their killing activities to autologous tumor cells. 
 Methods: Malignant pleural effusions were collected from 17 patients with lung adenocarcinoma. Mononuclear cells were isolated by Ficoll density gradient centrifugation and flow cytometer was used to analyze TIL cell components. TIL and tumor cells were separated through adherent culture. The tumor cells were identified via intramuscular injection of adherent cells into nude mice and the killing effect of cultured lymphocytes on autologous tumor cells was studied.
 Results: Of the TIL in malignant pleural effusions, T cells accounted for 60.6%-79.3%, while T helper cells were significantly higher than T killer cells (36.63%±1.90% vs 24.64%±2.32%, P<0.001). There were also natural killer (NK) cells and NK T cells in the effusions. Tumor cells were successfully isolated and cultured. The killing activity of cultured TIL to autologous tumor cells was 39.14%±12.04%, and the killing activity of TIL with high proliferation rate to autologous tumor cells was higher than that of low proliferation group (50.51%±3.80% vs 29.04%±5.77%, P<0.001).
 Conclusion: T lymphocytes are the major components of TIL in malignant pleural effusions derived from lung adenocarcinoma, and T helper cells are more than T killer cells. The killing activity of TIL with strong proliferation ability to autologous tumor cells is higher than that of TIL with weak proliferation ability. Therefore, cells from malignant pleural effusions could be used for cellular immunotherapy against tumor.
Adenocarcinoma of Lung ; Animals ; Cytotoxicity, Immunologic ; Humans ; Interleukin-2 ; Lung Neoplasms ; Mice ; Mice, Nude ; Pleural Effusion, Malignant ; T-Lymphocytes

Objective To explore the effect of micro-lectures withadvanced simulation man in improving the practical skills teaching of nursing students, so as to promote the students' post competency. Methods Totally the 186 nursing internswere divided into control group and observation group by random number method with 93 people in each group. The control group used the traditional teaching modein the teaching of practical skills.The observation group used the micro-lectures with high simulation teaching.Comparing the two groups of nursing students comprehensive assessment test simulation results in the theory, skills, scenarios, and nursing students the evaluation of the curriculum. Results The scores of the two groups were all above the qualification line, but the scores of the observation group were significantly higher than those of the control group (P<0.05). The theoretical examinations and the situation simulation comprehensive testswas(77.89 ± 7.79), (75.60 ± 7.92)points in control group, and (93.87 ± 3.90),(92.87 ± 4.08)points in observation group, there was significant difference between two groups (t=17.67, 18.70,all P=0.000). The curriculum evaluation results of improving learning initiative, active curriculum atmosphere, clear operation demonstration, exercise clinical thinking, improve the clinical interest were 81.72％(76/93), 72.04％(67/93), 93.55％(87/93), 60.22％(56/93), 67.74％(63/93)in control group, and 96.77％(90/93), 95.70％(89/93), 100.00％(93/93), 92.47％(86/93), 98.92％(92/93)in observation group, there was significant difference between two groups(χ2=20.39, P=0.016).Conclusions The effect is significant of micro-lectures combined with high simulation of human using in clinical skills teaching. Thismold can conducive to the cultivation of clinical thinking of nursing students, and improve clinical comprehensive ability, and promote the promotion of nursing students post competency.

Objective: To assess the anti-inflammatory and immunomodulatory activities of schisandrin B in mice. Methods: Kun-ming mice were randomly divided into 6 groups: the blank control group, the model control group, the positive control group( dexam-ethasone hydrochloride 5 mg·kg-1,levamisole hydrochloride 30 mg·kg-1), schisandrin B low (100 mg·kg-1), medium (200 mg ·kg-1) and high (400 mg·kg-1) dose groups. The inflammatory mice were induced by xylene and the immunosuppressed mice were induced by cyclophosphamide. The ear edema experiment, carbon clearance experiment and serum hemolysin experiment were per-formed with the swelling rate, swelling inhibition rate, carbon clearance index, phagocytic index, half hemolytic value, spleen index and thymus index as the investigation factors. Results: High dose and medium dose schisandrin B groups had significant inhibitory effect on mice ear edema when compared with the model control group(P<0. 05);There were significant differences between the high dose group and the positive group and there were significant differences in clearance index and phayxyitc index between high dose group and low dose group(P<0. 05), and high dose schisandrin B group had increased clearance index and phagocytic index in low immune function mice when compared with the model control group (P<0.05). Schisandrin B significantly increased HC50in low immune function mice when compared with the model control group (P<0. 01). Schisandrin B significantly increased the spleen index and thy-mus index in low immune function mice when compared with the model control group ( P<0. 01,and in a dose-dependent manner. Conclusion: Schisandrin B has significant anti-inflammatory and immune function enhancing effects.

Objective To evaluate the effect of resveratrol on inhibiting inflammatory responses of astrocytes and protecting neurons from injury in cerebral ischemic mice.Methods (1) Sixty adult male C57/BL6 mice were randomly assigned to sham-operated group,ischemic group (mice were subjected to transient global ischemia by bilateral common carotid artery occlusion after injection of 1.5％ DMSO),and resveratrol treatment group (mice were subjected to transient global ischemia after injection of 20 mg/kg resveratrol dissolved in 1.5％ DMSO),with 20 mice in each group;the neuronal damage in the ischemic mouse brains was analyzed by Cresyl Violet (CV) staining.(2) In vitro,the primary cultured astrocytes were divided into oxygen-glucose deprivation (OGD)-treated astrocytes group (DMSO was added into the culture medium of astrocytes followed by OGD treatment) and resveratrol-treated astrocytes group (DMSO and 25 μmol/L resveratrol were added into the culture medium of astrocytes followed by OGD treatment);the expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were analyzed by ELISA at re-oxygenation 12 h after 4 h-OGD treatment;meanwhile,the primary cultured neurons were injured by OGD treatment,and subsequently were divided into OGD-treated neurons group (neurons were incubated with culture medium of OGD-treated astrocytes) and resveratrol-treated neurons group (neurons were incubated with culture medium of resveratrol-trcated astrocytes).The neurite outgrowth and expressions of TNF-α and IL-6 in astrocytes of each group were analyzed by immunofluoresent staining,and the lactic dehydrogenase (LDH) release of OGD injured neurons was detected by LDH Kit.Results (1) CV staining showed that the degrees of hippocampal neurons injury in the resveratrol treatment group (1.50±0.34) were significantly decreased as compared with those in the ischemic group (3.17±0.31,P＜0.05).(2) ELISA showed that the expressions of TNF-α and IL-6 in the resveratrol-treated astrocytes group ([160.2±18.41] pg/mL,[241.25±42.22] pg/mL) were significantly decreased as compared with those in the OGD-treated astrocytes group ([282.4 ±17.01] pg/mL,[550.5 ±59.02] pg/mL,P＜0.05);LDH detection showed the release of LDH from the resveratrol-treated neurons (0.30±0.05) was dramatically decreased as compared with that from the OGD-treated neurons (0.62±0.08,P＜0.05);immunofluorescence staining showed that the TNF-α and lL-6 positive expressions in the resveratrol-treated astrocytes were obviously decreased as compared with those in the OGD-treated astrocytes,and the expressions of neurites of the resveratrol-treated neurons were obviously increased as compared with those in the OGD-treated neurons.Conclusion Resveratrol has neuroprotective effect on neurons in global cerebral ischemic mice,probably via down-regulating the expressions and secretion of inflammatory cytokines TNF-α and IL-6 from the astrocytes.