Objective To investigate the relationship between lumbosacral Modic changes(MCs)and their types and osteoporosis in patients with low back pain.Methods A total of 204 female patients with low back pain who were treated in Xuzhou City Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine from January 2021 to September 2024 were selected as study objects.According to magnetic resonance imaging results,the patients were divided into MCs group(139 cases)and non-MCs group(65 cases).MCs group was divided into type Ⅰ group(33 cases),type Ⅱgroup(90 cases)and type Ⅲ group(16 cases)according to MCs type.The age,hip bone mineral density(BMD)and T value of all groups were compared.Results There was no significant difference in age,hip BMD and T value between MCs group and non-MCs group(P＞0.05).Hip BMD and T values of four groups were type Ⅲ group＞non-MCs group＞type Ⅱ group＞typeⅠ group.Most of patients in type Ⅲ group had normal bone mass,and most of patients in other three groups had decreased bone mass or osteoporosis.There was no significant difference in hip BMD and T value between type Ⅱ group and non-MCs group(P＞0.05).The hip BMD and corresponding T values of patients in group Ⅲ significantly higher than the other three groups(P＜0.05).The hip BMD and corresponding T values of type Ⅰ patients were significantly lower than those of the other three groups(P＜0.05).Conclusion Patients with low back pain have different tendency of MCs with different bone mass.High bone mass(normal bone mass)is more likely to develop type Ⅲ MCs,and low bone mass or osteoporosis is more likely to develop type Ⅰ MCs.Anti-osteoporosis therapy for patients with type Ⅰ MCs should be emphasized.

Objective: To investigate the association between the parent-child relationship with depression among junior high school students, as well as the dual moderating effect of teacher support and gender, so as to provide a reference for depression interventions at family, school, and individual levels. Methods: In November 2024, a cluster sampling design was used to recruit 1 229 students from two junior high schools in Chengdu, Sichuan Province as participants. The survey employed the Interpersonal Relations Scale, the Patient Health Questionnaire-9 for depression, and the Students Perceived Teacher Support Behavior Questionnaire. Spearman s rank correlation analyses were used to examine the associations among the parent-child relationship, teacher support, gender, and depression. Through linear regression analysis, the dual moderating effects of teacher support and gender on the association between parent-child relationship and depression in middle school students were evaluated. Results: Scores for the parent-child relationship and for teacher support among middle school students were (4.03±1.00) and (4.23±0.86), respectively, and the depression score was 1.13 (1.00, 1.63). Parent-child relationships and teacher support showed positive correlations,while both the parent-child relationship and teacher support were negatively correlated with depression among junior high school students ( r =0.25, -0.55, -0.29, all P <0.01). Linear regression analyses showed that teacher support and gender jointly moderated the association between the parent-child relationship and depression. Further simple slopes analyses revealed that the negative impact of the parent-child relationship on depression was stronger in girls, peaking under low teacher support conditions ( β =-0.61); among boys, a negative effect was also present but weaker ( β =-0.48) (both P <0.05). Conclusion Teacher support and gender jointly moderated the association between the parent-child relationship and depression among junior high school students; girls depression are more closely linked to the quality of the parent-child relationship, while boys are more sensitive to changes in teacher support.

Objective To investigate the molecular mechanism of the induction of gastric cancer cell line AGS death by p97 inhibitor eeyarestatin Ⅰ(Eer Ⅰ).Methods AGS cells were treated with Eer Ⅰ.Then liquid chro-matography-mass spectrometry was used to perform proteome analysis for screening differentially expressed proteins and to find underlying signaling pathways.At the same time,the proteins of related pathway were investi-gated by protein immunoblotting.Cell proliferation was detected using the CCK-8 test kit;Cell apoptosis was detec-ted using TUNEL staining test kit;Liperfluo probe was used to detect ferroptosis-related lipid peroxides.Results In Eer Ⅰ treatment group,there were significant changes in proteins(fold change＞1.5 and P＜0.05),in which 125 proteins were increased and 132 proteins were decreased.The enrichment analysis of these DEPs showed that Eer Ⅰ might significantly affect cell apoptosis and ferroptosis-related signaling pathways.Furthermore,Eer Ⅰcould increase genomic DNA fragmentation related to cell apoptosis,increase of lipid peroxides in the ferroptosis pathway,causes changes in cell death related proteins,and inhibit the proliferation of gastric cancer cells.Conclusions p97 inhibitor Eer Ⅰ can induce cell apoptosis and ferroptosis in AGS cells,thereby inhibiting tumor cell proliferation.

Objective To investigate the correlation between the epidermal growth factor receptor(EGFR)gene polymorphisms and non-small cell lung cancer(NSCLC)in Yunnan Han population.Methods A total of 407 NSCLC patients and 526 healthy controls were included.Five SNPs(rs1050171,rs2072454,rs2227983,rs1140475 and rs2293347)in EGFR were genotyped using TaqMan assay.The association between SNPs and NSCLC,as well as pathological types,were analyzed.Results In dominant mode,rs2072454 C/T-T/T may be a risk factor for NSCLC(P=0.004;OR=1.50,95%CI 1.14～1.96).There was a significant difference in genotype frequency between the SCC group and the control group(P=0.007),but no difference in allele frequency after Bonferroni correction(P>0.01);In the dominant mode,C/T-T/T at this locus relative to C/C was a risk for AC(P=0.002;OR=1.86,95%CI 1.24～2.80).rs1050171 A/A had a significantly higher risk of SCC in recessive mode(P=0.006,OR=2.66,95%CI 1.33～5.33).In dominant mode,rs2227983 A/G-G/G/G was a risk factor for the development of SCC relative to A/A(P=0.007;OR=1.83,95%CI 1.15～2.89).Conclusion The SNPs rs2072454,rs1050171 and rs2227983 in EGFR gene may be associated with the risk of NSCLC development as well as the type of pathology in Yunnan Han population.

Objective To investigate the correlation between single nucleotide polymorphisms(SNPs)of the EGF gene,including rs11569017(A>T),rs2237051(A>G),rs3733625(C>T),and rs4444903(A>G),and the risk of non-small cell lung cancer(NSCLC)in a Han population of Yunnan.Methods A total of 439 patients with NSCLC and 520 healthy controls were recruited in Yunnan Province between January 2022 and December 2023.Genotyping of four SNP loci in the EGF gene was performed using TaqMan probes,followed by analysis of allele,genotype,genetic model,and haplotype distribution frequencies between NSCLC and control groups.Stratified analyses were further conducted based on NSCLC pathological types and clinical stages.Results The rs2237051 locus showed significant differences in allele(P=0.011)and genotype(P=0.042)frequencies between the squamous cell carcinoma(SCC)group and the control group.The frequency of the A allele was lower in the SCC group than in the control group(OR=0.71,95%CI 0.54～1.85),but there was no difference after Bonferroni correction(P>0.0125).Under the log-additive model,the rs2237051-2G/G+A/G genotype was associated with an increased risk of SCC(P=0.01;OR=1.42,95%CI 1.08～1.86).However,the association lost statistical significance after Bonferroni correction(P>0.0125).The haplotype rs11569017-rs2237051-rs3733625-rs4444903 has no difference between the two groups(P>0.0125).The stratified analysis revealed no significant associations between the genetic loci and different disease stages(P>0.0125).Conclusion In the Yunnan Han population,the individuals carrying the rs2237051-A allele of the EGF gene have a significantly lower risk of squamous lung cancer,but further functional experiments are needed to verify the protective mechanism.

This study was aimed at investigating the prevalence of Dabie bandavirus(DBV)infection in suspected cases of se-vere fever with thrombocytopenia syndrome(SFTS)and tick specimens in Cangzhou City from 2023 to 2024,and providing scientific basis for the prevention,control and pathogen surveillance of SFTS in this area.Pathogen detection and virus isolation were performed on suspected SFTS cases and tick specimens collected in Cangzhou City from 2023 to 2024.The next-generation sequencing technol-ogy was used to conduct whole-genome sequencing of DBV.A phylogenetic tree was constructed using software such as MEGA,DNA Star,and homology comparison and amino acid variation site analysis were carried out.Among the 70 suspected SFTS cases,the DBV positive detection rate was 60%(42/70).The positive cases were mainly farmers aged 50-79,concentrated in Yanshan County and Haixing County.402 ticks were pooled into 31 sample tubes,among which 2 tubes tested positive for DBV nucleic acid,with a mini-mum infection rate of 0.50%.A total of 9 human-derived and 2 tick-derived DBV strains were isolated.The whole-genome sequencing results showed that all strains clustered within the F branch,with sequences homology exceeding 99%.There were a total of 10 amino acid variation sites in the RdRP,GP and NSs sequences.Therefore,the distribution of SFTS cases in Cangzhou City exhibited regional clustering.The overall level of DBV infection in ticks in this region was relatively low.It is recommended to further improve the disease early warning mechanism and continue to conduct pathogen surveillance.

Objectives:To investigate the correlation between lumbar intervertebral disc degeneration and paraspinal muscle fat infiltration,abdominal fat,vertebral bone quality(VBQ)score as well as body mass index(BMI).Methods:A retrospective analysis was conducted on 280 patients who underwent lumbar MRI exami-nation at the affiliated Su Bei People's Hospital of Yangzhou University from August 2022 to March 2023.The patients included 159 males and 121 females,with ages ranging from 24 to 87 years(51.4±15.1 years).The L4/5 and L5/S1 intervertebral discs were classified according to the Pfirrmann grading system:grade Ⅱwas included in the normal group,and grades Ⅲ-Ⅴ were included in the degeneration group(no grade Ⅰpatients).The degree of paraspinal muscle fat infiltration was evaluated based on the modified Goutallier grad-ing system using MRI T2-weighted images.Abdominal fat indicators were measured,including abdominal di-ameter(AD),sagittal diameter(SAD),ventral subcutaneous thickness(VST),dorsal subcutaneous thickness(DST)at the level of the posterior margin of the L5/S1 disc,and visceral fat ratio(VFR).VBQ score was assessed us-ing MRI T1-weighted images(the average signal intensity of L1-L4 vertebrae divided by the signal intensity of cerebrospinal fluid at L3 level).Chi-square tests and independent sample t-tests were used to compare the differences in paraspinal muscle fat infiltration,abdominal fat,VBQ scores,and BMI between the two groups.Pearson and Spearman correlation analyses were performed to examine the correlations between lumbar inter-vertebral disc degeneration and paraspinal muscle fat infiltration,abdominal fat,VBQ scores,and BMI.Logis-tic regression analysis was conducted to determine the odds ratios(OR)and 95％confidence intervals(CI)for factors related to lumbar disc degeneration.Receiver operating characteristic(ROC)curves were further used to analyze the correlation between lumbar disc degeneration and paraspinal muscle fat infiltration,abdominal fat,and VBQ scores.Results:For the L4/5 intervertebral disc,107 cases were classified as grade Ⅱ(normal group)and 173 cases as grades Ⅲ-Ⅴ(degeneration group)according to the Pfirrmann classification;For the L5/S1 intervertebral disc,101 cases were grade Ⅱ(normal group)and 179 cases were grades Ⅲ-Ⅴ(degenera-tion group).There were statistically significant differences between L4/5 intervertebral disc degeneration and the degree of paravertebral muscle fat infiltration,AD,SAD,VFR,and VBQ scores(P＜0.05).L4/5 interverte-bral disc degeneration was positively correlated with the degree of paravertebral muscle fat infiltration,AD.SAD.VFR,and VBQ scores(r=0.412,r=0.244,r=0.234,r=0.244,r=0.254).L4/5 intervertebral disc degenera-tion was negatively correlated with DST(r=-0.139,P＜0.05),and there was no correlation between L4/5 inter-vertebral disc degeneration and BMI(P＞0.05).There were statistically significant differences between L5/S1 in-tervertebral disc degeneration and the degree of paravertebral muscle fat infiltration,AD,SAD,VFR,and VBQ scores(P＜0.05).L5/S1 intervertebral disc degeneration was positively correlated with the degree of par-avertebral muscle fat infiltration,AD.SAD,VFR,and VBQ scores(r=0.424,r=0.201,r=0.150,r=0.201,r=0.205).L5/S1 intervertebral disc degeneration was negatively correlated with DST(r=-0.175,P＜0.05),and there was no correlation between L5/S1 intervertebral disc degeneration and BMI(P＞0.05).Logistic regression analy-sis showed that L4/5 disc degeneration was closely related to SAD(OR=1.065),DST(OR=0.904),VBQ score(OR=2.143),and paraspinal muscle fat infiltration(OR=5.110).L5/S1 disc degeneration was closely related to DST(OR=0.889)and paraspinal muscle fat infiltration(OR=4.739).Conclusions:Lumbar disc degeneration is significantly correlated with paraspinal muscle fat infiltration,abdominal fat,and VBQ score.

This study was aimed at investigating the prevalence of Dabie bandavirus(DBV)infection in suspected cases of se-vere fever with thrombocytopenia syndrome(SFTS)and tick specimens in Cangzhou City from 2023 to 2024,and providing scientific basis for the prevention,control and pathogen surveillance of SFTS in this area.Pathogen detection and virus isolation were performed on suspected SFTS cases and tick specimens collected in Cangzhou City from 2023 to 2024.The next-generation sequencing technol-ogy was used to conduct whole-genome sequencing of DBV.A phylogenetic tree was constructed using software such as MEGA,DNA Star,and homology comparison and amino acid variation site analysis were carried out.Among the 70 suspected SFTS cases,the DBV positive detection rate was 60%(42/70).The positive cases were mainly farmers aged 50-79,concentrated in Yanshan County and Haixing County.402 ticks were pooled into 31 sample tubes,among which 2 tubes tested positive for DBV nucleic acid,with a mini-mum infection rate of 0.50%.A total of 9 human-derived and 2 tick-derived DBV strains were isolated.The whole-genome sequencing results showed that all strains clustered within the F branch,with sequences homology exceeding 99%.There were a total of 10 amino acid variation sites in the RdRP,GP and NSs sequences.Therefore,the distribution of SFTS cases in Cangzhou City exhibited regional clustering.The overall level of DBV infection in ticks in this region was relatively low.It is recommended to further improve the disease early warning mechanism and continue to conduct pathogen surveillance.

Objectives:To investigate the correlation between lumbar intervertebral disc degeneration and paraspinal muscle fat infiltration,abdominal fat,vertebral bone quality(VBQ)score as well as body mass index(BMI).Methods:A retrospective analysis was conducted on 280 patients who underwent lumbar MRI exami-nation at the affiliated Su Bei People's Hospital of Yangzhou University from August 2022 to March 2023.The patients included 159 males and 121 females,with ages ranging from 24 to 87 years(51.4±15.1 years).The L4/5 and L5/S1 intervertebral discs were classified according to the Pfirrmann grading system:grade Ⅱwas included in the normal group,and grades Ⅲ-Ⅴ were included in the degeneration group(no grade Ⅰpatients).The degree of paraspinal muscle fat infiltration was evaluated based on the modified Goutallier grad-ing system using MRI T2-weighted images.Abdominal fat indicators were measured,including abdominal di-ameter(AD),sagittal diameter(SAD),ventral subcutaneous thickness(VST),dorsal subcutaneous thickness(DST)at the level of the posterior margin of the L5/S1 disc,and visceral fat ratio(VFR).VBQ score was assessed us-ing MRI T1-weighted images(the average signal intensity of L1-L4 vertebrae divided by the signal intensity of cerebrospinal fluid at L3 level).Chi-square tests and independent sample t-tests were used to compare the differences in paraspinal muscle fat infiltration,abdominal fat,VBQ scores,and BMI between the two groups.Pearson and Spearman correlation analyses were performed to examine the correlations between lumbar inter-vertebral disc degeneration and paraspinal muscle fat infiltration,abdominal fat,VBQ scores,and BMI.Logis-tic regression analysis was conducted to determine the odds ratios(OR)and 95％confidence intervals(CI)for factors related to lumbar disc degeneration.Receiver operating characteristic(ROC)curves were further used to analyze the correlation between lumbar disc degeneration and paraspinal muscle fat infiltration,abdominal fat,and VBQ scores.Results:For the L4/5 intervertebral disc,107 cases were classified as grade Ⅱ(normal group)and 173 cases as grades Ⅲ-Ⅴ(degeneration group)according to the Pfirrmann classification;For the L5/S1 intervertebral disc,101 cases were grade Ⅱ(normal group)and 179 cases were grades Ⅲ-Ⅴ(degenera-tion group).There were statistically significant differences between L4/5 intervertebral disc degeneration and the degree of paravertebral muscle fat infiltration,AD,SAD,VFR,and VBQ scores(P＜0.05).L4/5 interverte-bral disc degeneration was positively correlated with the degree of paravertebral muscle fat infiltration,AD.SAD.VFR,and VBQ scores(r=0.412,r=0.244,r=0.234,r=0.244,r=0.254).L4/5 intervertebral disc degenera-tion was negatively correlated with DST(r=-0.139,P＜0.05),and there was no correlation between L4/5 inter-vertebral disc degeneration and BMI(P＞0.05).There were statistically significant differences between L5/S1 in-tervertebral disc degeneration and the degree of paravertebral muscle fat infiltration,AD,SAD,VFR,and VBQ scores(P＜0.05).L5/S1 intervertebral disc degeneration was positively correlated with the degree of par-avertebral muscle fat infiltration,AD.SAD,VFR,and VBQ scores(r=0.424,r=0.201,r=0.150,r=0.201,r=0.205).L5/S1 intervertebral disc degeneration was negatively correlated with DST(r=-0.175,P＜0.05),and there was no correlation between L5/S1 intervertebral disc degeneration and BMI(P＞0.05).Logistic regression analy-sis showed that L4/5 disc degeneration was closely related to SAD(OR=1.065),DST(OR=0.904),VBQ score(OR=2.143),and paraspinal muscle fat infiltration(OR=5.110).L5/S1 disc degeneration was closely related to DST(OR=0.889)and paraspinal muscle fat infiltration(OR=4.739).Conclusions:Lumbar disc degeneration is significantly correlated with paraspinal muscle fat infiltration,abdominal fat,and VBQ score.

Objective To investigate the relationship between lumbosacral Modic changes(MCs)and their types and osteoporosis in patients with low back pain.Methods A total of 204 female patients with low back pain who were treated in Xuzhou City Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine from January 2021 to September 2024 were selected as study objects.According to magnetic resonance imaging results,the patients were divided into MCs group(139 cases)and non-MCs group(65 cases).MCs group was divided into type Ⅰ group(33 cases),type Ⅱgroup(90 cases)and type Ⅲ group(16 cases)according to MCs type.The age,hip bone mineral density(BMD)and T value of all groups were compared.Results There was no significant difference in age,hip BMD and T value between MCs group and non-MCs group(P＞0.05).Hip BMD and T values of four groups were type Ⅲ group＞non-MCs group＞type Ⅱ group＞typeⅠ group.Most of patients in type Ⅲ group had normal bone mass,and most of patients in other three groups had decreased bone mass or osteoporosis.There was no significant difference in hip BMD and T value between type Ⅱ group and non-MCs group(P＞0.05).The hip BMD and corresponding T values of patients in group Ⅲ significantly higher than the other three groups(P＜0.05).The hip BMD and corresponding T values of type Ⅰ patients were significantly lower than those of the other three groups(P＜0.05).Conclusion Patients with low back pain have different tendency of MCs with different bone mass.High bone mass(normal bone mass)is more likely to develop type Ⅲ MCs,and low bone mass or osteoporosis is more likely to develop type Ⅰ MCs.Anti-osteoporosis therapy for patients with type Ⅰ MCs should be emphasized.