Age-related ovarian hypofunction includes a decrease in follicle quantity and quality as well as alterations in the ovarian microenvironment，the mechanisms of which are mainly related to mitochondrial dysfunction，free radical and antioxidant systems，telomere and telomerase alterations，and apoptosis，and is one of the major factors contributing to infertility in advanced maternal age （AMA）. Despite the tremendous progress in assisted reproductive technology in recent decades，few breakthroughs have been made in alleviating age-related ovarian hypofunction and improving reproductive outcomes for AMA. In recent years，there has been an increasing number of studies on the multi-level and multi-targeted mechanisms of traditional Chinese medicine （TCM） to improve age-related ovarian hypofunction by modulating mitochondrial homeostasis，alleviating oxidative stress，and inhibiting apoptosis，while more high-quality randomized controlled trials have demonstrated the clinical efficacy of TCM in assisted reproductive technology. Given this，this article presented a systematic review of recent research and randomized controlled trials on the mechanism of Chinese medicine active ingredients，single Chinese medicine， and Chinese medicine compounds in delaying age-related ovarian hypofunction，to clarify the current status and shortcomings of the research. This paper provides medication management of TCM for effectively alleviating age-related ovarian hypofunction and improving reproductive outcomes for AMA.

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder in women of childbearing age. Patients with PCOS are more likely to have psychological disorders such as anxiety and depression.In recent years, researchers have explored the link between PCOS and depression and anxiety. The biochemical changes and psychological stress experienced by PCOS patients caused by different clinical and metabolic phenotypes and long-term complications may account for the high levels of depression and anxiety experienced by women with PCOS risk. The purpose of this review was to summarize the present findings on PCOS phenotype and long-term complications related to depression and anxiety, to clarify the factors that may contribute to depression and anxiety, and to formulate future study directions and clinical practice.

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder in women of childbearing age. Patients with PCOS are more likely to have psychological disorders such as anxiety and depression.In recent years, researchers have explored the link between PCOS and depression and anxiety. The biochemical changes and psychological stress experienced by PCOS patients caused by different clinical and metabolic phenotypes and long-term complications may account for the high levels of depression and anxiety experienced by women with PCOS risk. The purpose of this review was to summarize the present findings on PCOS phenotype and long-term complications related to depression and anxiety, to clarify the factors that may contribute to depression and anxiety, and to formulate future study directions and clinical practice.

Endometriosis is a chronic gynecological disease that affects women of childbearing age and may lead to infertility. With the progress of medical technology, there are more and more studies on endometriosis, but the technical methods that can be used to evaluate the disease are lack of sensitivity, specificity and accuracy, so they still face difficulties in diagnosis and treatment. Proteomics can not only study diseases from the perspective of physiology and pathology, but also play an important role in understanding the different proteins involved in the development of diseases and their changes. Follicular fluid is an important secretion containing many kinds of proteins produced by oocytes, granulosa cells and theca cells in human body. Therefore, this review summarizes the research progress of endometriosis from the study of proteomics in follicular fluid.

In vitro fertilization (IVF) is a routine treatment for infertility diseases, but there are low pregnancy success rates and corresponding adverse pregnancy outcomes. In the post-gene era, human follicular fluid studies based on proteomics can provide substantial information about the various steps of the IVF process and contribute to predict or improve IVF pregnancy outcomes. By searching the PubMed, Scopus, and Web of Science databases, this paper reviews recent advances in studies related to IVF pregnancy outcomes based on human follicular fluid proteomics. The aim is to summarize the potential markers that can be used to optimize IVF outcomes while providing insights into IVF failure mechanisms, which can facilitate basic research in reproductive medicine, thereby accelerating clinical transformation.

Endometriosis is a chronic gynecological disease that affects women of childbearing age and may lead to infertility. With the progress of medical technology, there are more and more studies on endometriosis, but the technical methods that can be used to evaluate the disease are lack of sensitivity, specificity and accuracy, so they still face difficulties in diagnosis and treatment. Proteomics can not only study diseases from the perspective of physiology and pathology, but also play an important role in understanding the different proteins involved in the development of diseases and their changes. Follicular fluid is an important secretion containing many kinds of proteins produced by oocytes, granulosa cells and theca cells in human body. Therefore, this review summarizes the research progress of endometriosis from the study of proteomics in follicular fluid.

In vitro fertilization (IVF) is a routine treatment for infertility diseases, but there are low pregnancy success rates and corresponding adverse pregnancy outcomes. In the post-gene era, human follicular fluid studies based on proteomics can provide substantial information about the various steps of the IVF process and contribute to predict or improve IVF pregnancy outcomes. By searching the PubMed, Scopus, and Web of Science databases, this paper reviews recent advances in studies related to IVF pregnancy outcomes based on human follicular fluid proteomics. The aim is to summarize the potential markers that can be used to optimize IVF outcomes while providing insights into IVF failure mechanisms, which can facilitate basic research in reproductive medicine, thereby accelerating clinical transformation.

Objective To observe the effects of herb-partition moxibustion at navel on the expressions of Bcl-2 and Bax in rats with premature ovarian failure (POF); To explore its mechanism underlying improvement of POF. Methods POF model was established by intraperitoneal injection of cyclophosphamide. Forty-eight Female Wistar rats were randomly divided into normal group, model group, moxibustion group and Western medicine group, with 12 rats in each group. Rats of moxibustion group were treated with herb-partition moxibustion at navel (Shenque, CV8), 30 min/d, once every other day; rats of Western medicine group were given intragastric dose of estradiol valerate, once a day, for 14 d. The protein and mRNA expressions of Bax and Bcl-2 in ovarian granulosa cells were detected by immunohistochemal method, Western blot and qRT-PCR, respectively.Results The weights of ovaries in the model group rats were significantly lower than those in the normal group (P<0.05). The expressions of Bcl-2 and Bax protein in ovarian tissue of each group were mainly expressed brown coloration and in the cytoplasm of cells. The range of Bax protein in granulosa cells of moxibustion group and Western medicine group was significantly less than that in model group. The range of Bcl-2 in moxibustion group and Western medicine group was significantly larger than that in model group. Compared with the normal group, the expressions of Bcl-2 protein and mRNA in the model group rats significantly decreased (P<0.05,P<0.01), and the expression of Bax protein and mRNA significantly increased (P<0.05,P<0.01). The expression level of Bcl-2 protein and mRNA was significantly higher in the moxibustion group and in the western medicine group than that in the model group (P<0.05,P<0.01). The expression level of Bax protein and mRNA in the moxibustion group was significantly lower than that in the model group (P<0.05,P<0.01). ConclusionThe herb-partition moxibustion at navel can improve the function of ovaries by up regulating the expression of Bcl-2 and down regulating the expression of Bax in POF rats model.