DNA methylation is a prevalent epigenetic modification in vertebrates, and it has been shown to be involved the regulation of gene expression and embryo development. However, it remains unclear how DNA methylation regulates sexual development, especially in species without sex chromosomes. To determine this, we utilized zebrafish to investigate DNA methylation reprogramming during juvenile germ cell development and adult female-to-male sex transition. We reveal that primordial germ cells (PGCs) undergo significant DNA methylation reprogramming during germ cell development, and the methylome of PGCs is reset to an oocyte/ovary-like pattern at 9 days post fertilization (9 dpf). When DNA methyltransferase (DNMT) activity in juveniles was blocked after 9 dpf, the zebrafish developed into females. We also show that Tet3 is involved in PGC development. Notably, we find that DNA methylome reprogramming during adult zebrafish sex transition is similar to the reprogramming during the sex differentiation from 9 dpf PGCs to sperm. Furthermore, inhibiting DNMT activity can prevent the female-to-male sex transition, sug-gesting that methylation reprogramming is required for zebrafish sex transition. In summary, DNA methylation plays important roles in zebrafish germ cell development and sexual plasticity.

Objective To investigate the synergistic regulation of KDM3B and JMJD1C in leukemia. Methods The expression level of JMJD1C and KDM3B were analyzed in multiple acute myeloid leukemia (AML) cell lines. AML cell lines NB4 and HL-60 were treated with Daminozide, followed by determination of H3K9 mono-methylation and di-methylation. AML cell lines NB4 and HL-60 were treated with Daminozide, ATRA (retinoid acid All-trans), C Vitamin and the expression of KDM3B and JMJD1C were detected by real-time quantitative PCR. Results The expression level of KDM3B and JMJD1C in the AML cell lines was negatively correlated. In NB4 and HL-60 cells treated by daminozide, H3K9 mono-methylation and di- methylation level showed a rising trend in these two cell groups. After treatment of NB4 cells with the 3 reagents, the level of mRNA of KDM3B was down-regulated while the level of mRNA of JMJD1C was up-regulated. In HL-60 cells treated by daminozide, the mRNA level of KDM3B was up-regulated and the mRNA level of JMJD1C was down-regulated. Conclusion The expression of KDM3B and JMJD1C is negatively correlated in patients with AML.

Objective To study the prevalence and socio-demographic correlates of mental disorders in Beijing residents. Methods The multi-stage stratified cluster random sampling method was used,19 874 residents aged 18 or above who had lived for more than six months in Beijing were selected. Face-to-face assessment was conducted by trained investigators by using the Chinese version of the Structured Clinical Interview for DSM-ⅣAxisⅠDisorders-Patient Edition(SCID-Ⅰ/P)to find any mental disorders, and the Mini-Mental State Examination (MMSE) to screen for dementia and mental retardation. Those who were positive on MMSE(MMSE≤17 for those who completed elementary education or less,≤23 for those who completed middle school or above) were further assessed to confirm dementia and mental retardation by using the SCID. Results 16 032 (80.7%) out of 19 874 eligible residents completed the face-to-face assessment. Adjusted by age and gender, the lifetime prevalence of all mental disorders was 120.8‰ (1 937/16 032, 115.8‰-125.9‰), and the top three most common ones were major depressive disorder (527,32.9‰), alcohol dependence and abuse (311, 19.4‰), and anxiety disorder, NOS (270, 16.8‰). The point prevalence of all mental disorders was 75.3‰ (1 207/16 032,71.2‰-79.4‰), the top three were specific phobias (187, 11.7‰),anxiety disorder, NOS (186, 11.6‰), and major depressive disorder (162, 10.1‰).The prevalence of mental disorders was significantly higher in the elderly(OR=1.014),female(OR=1.428),unemployed(OR=1.096),people having poor rapports with family(OR=1.686) or others(OR=1.901), smoking(OR=1.129)or drinking(OR=1.262). The prevalence of mental disorders was significantly lower in the urban residents(OR=0.840), people in a higher level of education(OR=0.813), people who had got married/remarried or who had partner(OR=0.689), people who had no family history of any mental disorders(OR=0.405). Conclusions Approximately 12% of Beijing residents may meet at least one diagnosis of mental disorder in their lifetime; The prevalence of mental disorders is associated with older age,female gender,lower level of education,rural dwelling,positive family history of mental disorders,and poor social support system.

Objective To study the prevalence and socio-demographic correlates of mental disorders in Beijing residents. Methods The multi-stage stratified cluster random sampling method was used,19 874 residents aged 18 or above who had lived for more than six months in Beijing were selected. Face-to-face assessment was conducted by trained investigators by using the Chinese version of the Structured Clinical Interview for DSM-ⅣAxisⅠDisorders-Patient Edition(SCID-Ⅰ/P)to find any mental disorders, and the Mini-Mental State Examination (MMSE) to screen for dementia and mental retardation. Those who were positive on MMSE(MMSE≤17 for those who completed elementary education or less,≤23 for those who completed middle school or above) were further assessed to confirm dementia and mental retardation by using the SCID. Results 16 032 (80.7%) out of 19 874 eligible residents completed the face-to-face assessment. Adjusted by age and gender, the lifetime prevalence of all mental disorders was 120.8‰ (1 937/16 032, 115.8‰-125.9‰), and the top three most common ones were major depressive disorder (527,32.9‰), alcohol dependence and abuse (311, 19.4‰), and anxiety disorder, NOS (270, 16.8‰). The point prevalence of all mental disorders was 75.3‰ (1 207/16 032,71.2‰-79.4‰), the top three were specific phobias (187, 11.7‰),anxiety disorder, NOS (186, 11.6‰), and major depressive disorder (162, 10.1‰).The prevalence of mental disorders was significantly higher in the elderly(OR=1.014),female(OR=1.428),unemployed(OR=1.096),people having poor rapports with family(OR=1.686) or others(OR=1.901), smoking(OR=1.129)or drinking(OR=1.262). The prevalence of mental disorders was significantly lower in the urban residents(OR=0.840), people in a higher level of education(OR=0.813), people who had got married/remarried or who had partner(OR=0.689), people who had no family history of any mental disorders(OR=0.405). Conclusions Approximately 12% of Beijing residents may meet at least one diagnosis of mental disorder in their lifetime; The prevalence of mental disorders is associated with older age,female gender,lower level of education,rural dwelling,positive family history of mental disorders,and poor social support system.

Objective To explore the value of follow-up of serum TSH stimulating thyroglobulin (Tg) test and 131I whole body scan(WBS) in patients with differentiated thyroid cancer(DTC) who were treated by 131I.Methods By SPSS software,Kappa test was performed between results of serum Tg,TgAb test and WBS for diagnosis of persistent/recurrent DTC.Results Consistency test of TSH stimulating Tg,TgAb and 131I WBS for diagnostic persistent/recurrent or metastasis of DTC:kappa value =0.587,SE =0.076,P ＜ 0.01.Although the results showed that two measurements appeared definite consistency,it is dissatisfactory.Conclusion Serum TSH stimulating Tg test and 131I whole body scan(WBS) are important follow-up tools for patients with DTC.Because of Tg interfering it is necessary to performed TgAb test simultaneously,when Tg test was performed.Especially 131I WBS do is not absent because false negative would be able to appeared in single serum TSH stimulating Tg and TgAb test.