Objective:To investigate the diagnosis and treatment of pre-excitation syndrome in civil aircrews, and to provide data for aeromedical assessment of this disease.Methods:The clinical data of 20 civil aircrews was retrospectively analyzed who were diagnosed with pre-excitation syndrome during the annual health checkup at a civil aviation physical examination institution between April 2022 and June 2023. The pathogenesis, electrocardiographic manifestations, diagnosis and treatment, and results of aeromedical assessment of pre-excitation syndrome were summarized.Results:Among the 20 civil aircrews detected with pre-excitation syndrome, 1 case showed apical hypertrophic cardiomyopathy on echocardiography, which was identified as unqualified for flight missions by aeromedical assessment. Supraventricular tachycardia or ventricular tachycardia occurred in 3 cases, 2 of whom were determined as qualified after specially-permitted assessment, but their duration of flight and duties as a captain were restricted. One security guard was determined as qualified. One aircrew and one security guard developed symptoms of palpitation caused by paroxysmal supraventricular tachycardia, but were identified as qualified after radiofrequency ablation. The other 14 aircrews had no symptoms of palpitation, nor was arrhythmia revealed by Holter. Aeromedical assessment concluded that they were eligible for flight.Conclusions:The aeromedical assessment of pre-excitation syndrome among civil aircrews should be individualized and based on 24 h Holter findings, treadmill exercise test, echocardiography, cardiac electrophysiology and other examinations.

Objective:To investigate the diagnosis and treatment of pre-excitation syndrome in civil aircrews, and to provide data for aeromedical assessment of this disease.Methods:The clinical data of 20 civil aircrews was retrospectively analyzed who were diagnosed with pre-excitation syndrome during the annual health checkup at a civil aviation physical examination institution between April 2022 and June 2023. The pathogenesis, electrocardiographic manifestations, diagnosis and treatment, and results of aeromedical assessment of pre-excitation syndrome were summarized.Results:Among the 20 civil aircrews detected with pre-excitation syndrome, 1 case showed apical hypertrophic cardiomyopathy on echocardiography, which was identified as unqualified for flight missions by aeromedical assessment. Supraventricular tachycardia or ventricular tachycardia occurred in 3 cases, 2 of whom were determined as qualified after specially-permitted assessment, but their duration of flight and duties as a captain were restricted. One security guard was determined as qualified. One aircrew and one security guard developed symptoms of palpitation caused by paroxysmal supraventricular tachycardia, but were identified as qualified after radiofrequency ablation. The other 14 aircrews had no symptoms of palpitation, nor was arrhythmia revealed by Holter. Aeromedical assessment concluded that they were eligible for flight.Conclusions:The aeromedical assessment of pre-excitation syndrome among civil aircrews should be individualized and based on 24 h Holter findings, treadmill exercise test, echocardiography, cardiac electrophysiology and other examinations.

Objective:To investigate the different killing effects on human hilar cholangiocarinoma cells FRH with cytosine deaminase(CD) and herpes simplex virus thymidine kinase(HSV tk)double suicide genes coexpression compared with single gene mediated by retrovirus.To find a more efficient and low toxicity suicide gene therapy for hilar cholangiocarinoma.Methods:CD and HSV tk double suicide genes were transfected into PA317 cells using lipofectamine.The positive clones were picked out and cultured after G418 selected.The viral supernatant was collected.The FRH cells were infected with the virus containing the double suicide genes.After G418 selection,RT PCR was resorted to demonstrated the successful transcription of CD and HSV tk genes.The FRH/CD+tk and FRH cells in culture were respectively treated with 5 Fc and /or GCV.The cytoxicity efficacy was evaluated by microculture tetrajolium test (MTT) method.Results:The virus containing double suicide gene was produced in PA317 cells.Double suicide genes were stably expressed in RFH cells after being infected with the virus.The killing effect of combination 5 Fc with GCV on FRH/CD+tk cells is more effective than that of using 5 Fc or GCV alone.Conclusion:The CD+TK/5 Fc+GCV co expression system is more effective for killing effects on FRH cells than that of CD/5 Fc or tk/GCV system alone.

Objective: To investigate effect of exogenous wild-type p53 gene on the cell growth and tumorigenicity of human gallbladder cancer cell lines. Methods: After identification of the genetic status of p53 gene of GBC-SD cell lines with the immunocytochemistry staining and the direct sequencing technique of PCR products, eukaryotic expressing plasmid pCMV-p53 was introduced by lipofectamine-mediated into GBC-SD cell lines. Growing transfected cells were selected by G418. The presence and expression of exogenous p53 gene was detected by PCR, RT-PCR and Western blot. The cellular proliferating ability was assessed using the cell growth curve and cloning assay. The xenograft in nude mice was performed to examine the effect of tumorigenicity. Results: P53 protein overexpression was showed in GBC-SD cell lines. A transversion of TAC→AAC at codon 126 of exon 5 was confirmed. PCR, RT-PCR and Western blot showed exogenous p53 gene had successfully transfected into GBC-SD cells and obtained high expression. The growth and proliferation of the cells were greatly decreased, and the tumorigenicity was significantly inhibited after transfection wtp53. Conclusion: The expression of exogenous wild-type p53 gene could effectively inhibit the growth of gallbladder cancer GBC-SD cells in vitro and in vivo.

OBJECTIVE:To study the mechanism by which L-arginine regulates hypoxic pulmonary vascular structural remodeling.METHODS:Eighteen Wistar rats were randomly divided into control group,hypoxic group and hypoxic+L-arginine group.Pulmonary artery pressure was measured with right cardiac catheterization.Micro-structure and ultra-structure of pulmonary tissue were observed and collagen I expression was evaluated with immunohistochemistry.RESULTS:Mean pulmonary artery pressure(mPAP) was(2.7?0.3)kPa in hypoxic rats and(2.1?0.1)kPa in control rats(P