Objective To explore the efficacy and underlying mechanism of Astragalus in the treatment of viral pancreatitis using network pharmacology,with confirmation of its efficacy and mechanism in cell experiments.Methods Astragalus and viral pancreatitis targets obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)and GeneCards databases were combined to obtain intersection targets.GO functional enrichment and KEGG signaling pathway enrichment analyses of therapeutic targets were conducted using the Database for Annotation,Visualization,and Integrated Discovery(DAVID)database.The interactions between therapeutic targets were analyzed using the STRING database and Cytoscape 3.10.2,and the core therapeutic targets were screened.Molecular docking between the most effective therapeutic components and the core targets was performed using PyMOL 3.0 and AutoDock Tools 1.5.7.CVB3 was used to construct a viral pancreatitis cell model for verification of the core targets.Results Seventy-eight therapeutic targets were identified.Enrichment analyses revealed the possible involvement of pathways related to cancer,lipids and atherosclerosis,and PI3K-AKT signaling.AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9 were identified as possible core targets.The result of cell experiments showed that the expression level of AMY was significantly increased in the model group(P＜0.05).The Astragalus injection group exhibited significantly decreased expression levels of AMY,AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9 compared with the model group(P＜0.05).Conclusions Astragalus injection effectively treated viral pancreatitis,and its therapeutic mechanism may involve reduced expression levels of AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9.

Objective To explore the efficacy and underlying mechanism of Astragalus in the treatment of viral pancreatitis using network pharmacology,with confirmation of its efficacy and mechanism in cell experiments.Methods Astragalus and viral pancreatitis targets obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)and GeneCards databases were combined to obtain intersection targets.GO functional enrichment and KEGG signaling pathway enrichment analyses of therapeutic targets were conducted using the Database for Annotation,Visualization,and Integrated Discovery(DAVID)database.The interactions between therapeutic targets were analyzed using the STRING database and Cytoscape 3.10.2,and the core therapeutic targets were screened.Molecular docking between the most effective therapeutic components and the core targets was performed using PyMOL 3.0 and AutoDock Tools 1.5.7.CVB3 was used to construct a viral pancreatitis cell model for verification of the core targets.Results Seventy-eight therapeutic targets were identified.Enrichment analyses revealed the possible involvement of pathways related to cancer,lipids and atherosclerosis,and PI3K-AKT signaling.AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9 were identified as possible core targets.The result of cell experiments showed that the expression level of AMY was significantly increased in the model group(P＜0.05).The Astragalus injection group exhibited significantly decreased expression levels of AMY,AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9 compared with the model group(P＜0.05).Conclusions Astragalus injection effectively treated viral pancreatitis,and its therapeutic mechanism may involve reduced expression levels of AKT1,TP53,HIF1A,CASP3,IL-6,and MMP9.

Objective To perform an analytical comparison of CT findings between sarcomatoid clear cell renal cell carcinoma(sccRCC)and grade 4 clear cell renal cell carcinoma(ccRCC),to improve preoperative diagnosis for sccRCC.Methods The pathology and imaging data of 18 patients with sccRCC and 23 patients with grade 4 ccRCC were retrospectively analyzed,the differences of non-contrast and contrast-enhanced CT features between the two groups were compared.Results(1)sccRCC and ccRCC demonstrated similar features regarding hemorrhage,calcification,retroperitoneal lymph node enlargement,distant metastasis,invasion of perinephric organs,vascular invasion,and neovascularization around and within the tumor,as well as CT values at different phases.No statistically significant differences were observed between the two groups(P＞0.05).(2)In the sccRCC,solid components were predominant in 6 cases,compared to 16 cases in the ccRCC,showed a significant difference(P=0.017).The ratio of cystic and necrotic areas in sccRCC was 0.66±0.20,and that in ccRCC was 0.51±0.17,with a statistically significant difference(P=0.015).(3)Using a cystic and necrotic area ratio of 0.63 as the diagnostic threshold yields the optimal discriminative performance,with an area under the curve(AUC)of 0.732,a sensitivity of 0.611,and a specificity of 0.826.Conclusion sccRCC is distinguished by a significantly higher proportion of cystic and necrotic elements as opposed to the grade 4 ccRCC.

Objective To perform an analytical comparison of CT findings between sarcomatoid clear cell renal cell carcinoma(sccRCC)and grade 4 clear cell renal cell carcinoma(ccRCC),to improve preoperative diagnosis for sccRCC.Methods The pathology and imaging data of 18 patients with sccRCC and 23 patients with grade 4 ccRCC were retrospectively analyzed,the differences of non-contrast and contrast-enhanced CT features between the two groups were compared.Results(1)sccRCC and ccRCC demonstrated similar features regarding hemorrhage,calcification,retroperitoneal lymph node enlargement,distant metastasis,invasion of perinephric organs,vascular invasion,and neovascularization around and within the tumor,as well as CT values at different phases.No statistically significant differences were observed between the two groups(P＞0.05).(2)In the sccRCC,solid components were predominant in 6 cases,compared to 16 cases in the ccRCC,showed a significant difference(P=0.017).The ratio of cystic and necrotic areas in sccRCC was 0.66±0.20,and that in ccRCC was 0.51±0.17,with a statistically significant difference(P=0.015).(3)Using a cystic and necrotic area ratio of 0.63 as the diagnostic threshold yields the optimal discriminative performance,with an area under the curve(AUC)of 0.732,a sensitivity of 0.611,and a specificity of 0.826.Conclusion sccRCC is distinguished by a significantly higher proportion of cystic and necrotic elements as opposed to the grade 4 ccRCC.

Objective To investigate the correlation between preoperative MR imaging features and the incidence of tumor metastasis in clear cell renal cell carcinoma.Methods The clinical and preoperative MR imaging data of 64 patients with clear cell renal cell carcinoma were analyzed retrospectively.According to the occurrence of metastasis,the patients were divided into non-metastasis group(n=42)and metastasis group(n=22).The clinical and imaging features of the two groups were analyzed with univariate analysis and multivariate logistic regression.Results The results of the univariate analysis showed that among the clinical and preoperative MR imaging data,there was no significant difference between the two groups in gender,tumor location and intra-tumoral cystic changes(P>0.05),but the patient's ages,clinical symptoms,tumor sizes,necrosis,capsule breakthrough,low signal nodules in T2WI,venous thrombosis,TNM stages,and Fuhrman grades were significantly different between two groups(P<0.05).Multivariate logistic regression analysis showed that low signal nodules in T2WI was an independent predictor of metastasis of clear cell renal cell carcinoma(P=0.028).Combined with diffusion weighted imaging(DWI)sequence,the average apparent diffusion coefficient(ADC)in related areas was measured.The ADC value of low signal nodules area was(0.541±0.101)×10-3 mm2/s in the metastasis group,and the ADC value of non-low signal nodules area was(0.972±0.113)×10-3 mm2/s(P<0.001).Conclusion The metastasis of clear cell renal cell carcinoma is often accompanied by low signal nodules in T2WI in tumors.Combined with the lower ADC value,they can be used as the characteristic imaging features to effectively evaluate the risk of metastasis of clear cell renal cell carcinoma.

Purpose To investigate the value of MRI multi-sequence-based radiomic nomogram in identifying clear cell renal cell carcinoma from fat-poor renal angiomyolipoma in small renal masses(≤4 cm).Materials and Methods A retrospective analysis was performed for 78 renal masses in 75 patients with pathologically confirmed cases in the First Affiliated Hospital of Nanjing Medical University from July 2017 to December 2022,including 56 cases of renal clear cell carcinoma and 22 cases of fat-deficient angiomyolipoma,and all participants were divided into a training set(n=55)and a validation set(n=23)in a ratio of 7∶3.Radiomics features were extracted from T2WI and diffusion-weighted imaging sequences,and the t-test and minimum absolute shrinkage and selection algorithm were used for feature selection,the radiomics model was constructed,and the radiomics score was calculated.The clinical characteristics and subjective characteristics of MRI were evaluated to establish a clinical model,and the radiomics nomogram was constructed based on the radiomics score and clinical features,and the calibration,discrimination and clinical practicability of the nomogram were evaluated.Results A total of2 632 radiomics features were extracted from each patient,and 4 features were used to construct a radiomics model.The radiomics model had good discrimination ability in the training set[area under the curve(AUC)=0.979,95％CI 0.937-1.000)]and the validation set(AUC=0.833,95％CI 0.626-1.000).The radiomics nomogram had good calibration and discrimination ability in the training set(AUC=0.988,95％CI 0.963-1.000)and validation set(AUC=0.867,95％CI 0.698-1.000),which was better than the clinical model(AUC=0.725,95％CI 0.478-0.972)and radiomics model(AUC=0.833,95％CI 0.626-1.000)in the test set.Decision curve analysis showed that the clinical utility of nomogram was better than that of clinical factor model and radiomics features.Conclusion MRI-based radiomics nomogram combined with radiomics scores and clinical factors can be used to non-invasively distinguish clear cell renal cell carcinoma from alipid-deficient angiomyolipoma before surgery.

Objective To study the relationship of TN-C,MMP-9 and TGF-β1 expression with aorta atherosclerotic plaue stability in mice on long-term high fat diet.Methods Fifty male apo E/ mice on high fat diet served as an experimental group and 50 male C57BL/6 mice on basic diet served as a control group.The morphology of plaques was observed with HE staining and the expression of TN-C,MMP-9 and TGF-β1 was detected with immunohistochemical staining.Results The serum TC and LDL-C levels were significantly higher in experimental group than in control group at weeks 16,24,32 and 40 (P＜0.05).The serum TG level was significantly higher in experimental group than in control group at week 16 (P＜0.05) and was significantly lower in experimental group than in control group at week 40 (P＜0.05).With the lengthening of the feeding time,the plaque area,the ratio of plaque to lumen area,and the expression of TN-C and MMP-9 increased gradually,but the expression of TGF-β1 decreased gradually (P＜0.05).Conclusion The expression of TN-C,MMP-9 and TGF-β1 can show the stability of atherosclerotic plaques.

Objective To observe the formation process with 3.0 T MRI dynamically, and to discuss the feasibility of molecular imaging studies on restenosis. Methods The models were built with balloon (2.0 F) injury which were separated into restenosis group (n=48) and control group (n=48). Zero h, 24 h, 1 week, 2 week, 4 week and 8 week after surgery, 3.0 T MRI scanning (T1WI, T2WI, PDWI) was performed respectively, the vascular of injured side were obtained for HE staining to observe the pathological changes, to analyze the measurement of neointimal area (IA), intimal proliferation index (IHI), lumen area (LA) and stenosis rates, correlation between HE staining measurements and MR images were analyzed. Two weeks after the injury, the restenosis model of rats (n=8) and control rats (n=8) were injected ultrasmall superparamagntiec iron oxide (USPIO,1 mmol/kg) by tail vein, respectively. 3.0 T MRI scanning (T2WI) was underwent at 0 h and 24 h after injection, the change of the arterial wall T2 signal was quantitatively analyzed and the relative signal intensity (rSI) and relative change rate (rSIC) of the vessel wall were calculated. Reference to MRI images, corresponding line segments were taken for Perl's blue staining and immunohistochemically staining of macrophages. One-way ANOVA, Pearson and t test were used for statistical analysis. Results In the early?term (0 h,24 h), the wall and surrounding high signal organization boundary was not clear, there was no obvious morphological change of the lumen. In the medium?term (1, 2 week), signal of the injured wall increased with different extents, wall thickening and luminal narrowing was progressive, the inwall was coarse. In the later?term (4, 8 week) wall signal got slightly lower, wall thickness, lumen change were not significant, the wall area and LA were significantly associated with pathologic measurement result (r value were 0.978, 0.732; P<0.05). In the control group, signal of wall and lumen morphological change were not significant among the different time points. IA were (0.131 ± 0.011) mm2, (0.588 ± 0.017) mm2, (1.061 ± 0.033) mm2, (1.192 ± 0.034) mm2;1, 2, 4, 8 week after injury, respectively, IHI were 0.235 ± 0.022, 0.578 ± 0.013, 0.715 ± 0.011, 0.737 ± 0.009, respectively, stenosis rates were (5.586 ± 0.987)%, (25.395 ± 1.112)%, (40.019 ± 1.298)%, (41.890 ± 0.951)%, respectively, difference between groups were statistically (P<0.05). In the control group, there was no significant differences of medium area, luminal stenosis and neointimal formation respectively at different time points (P>0.05). rSI was 1.582±0.051 after the injection of USPIO, then 24 h after injection of USPIO, T2 signal of the vessel wall was reduced significantly, rSI was 1.260 ± 0.088, rSIC was (-20.249 ± 6.489) % with statistical difference (t value was 8.924,P<0.05). But there was no statistical difference in control rats (P>0.05). Perl's staining combined with immunohistochemical staining confirmed that the iron particles were taken by the macrophage's phagocytosis just in the neointimal. Conclusion 3.0 T MRI is capable of demonstrating the vessel wall and lumen changes dynamically, and the measurements are correlated with pathological results. USPIO can be consumed by macrophages in the neointimal, resulting in T2 signal of the vessel wall decreased significantly.

Objective To explore the feasibility of 7.0T MR scanner in mouse aorta atherosclerosis models.Visualising the TN-C in atherosclerotic plaque by immunohistochemistry and its correlation with CD68 to provide experimental basis for the feasibility of TN-C in targeted MRI.Methods ApoE-/- mice and wild type C57 mice were fed on high fat diet to establish aorta atherosclerosis model (n=10),the aorta were observed by MRI after 14 weeks.The aorta specimens were taken to stain with HE to observe the pathological changes.The plaque was stained with oil red O,anti-TNC and TN-C antibody respectively to observe the fat,CD68 and TN-C in plaque.Results 7.0 MRI showed the aortic wall of the experimental group was thicker,high signal on T1 WI and PDWI,and low signal on T2 WI after 14 weeks.The histopathlogic examination showed the intima was obviously thicker,and the lumen was ir-regulary narrow.Both of CD68 and TN-C were highly expressed in plaque,and the distribution of TN-C correlated with CD68.In the control group,no case showed hyper-signal in the vessel wall of aorta or narrow lumen by MRI,and the histopathlogy showed no for-mation of atherosclerotic plaque in the aorta.Conclusion Aorta atherosclerotic plaque can be established through high fat diet on ApoE-/- mouse,and 7.0 MR can successfully detect it.TN-C is high expressed in AS plaque and the expression is correlated with CD68,which may suggest that they may collaborate in the development of AS.Detecting TN-C could be useful for the further study of atherosclerotic plaque.

The non-human primates have been profoundly being used to study the human reproductive models .It is the prerequisites to set up the operating rooms including hardware and software on reproductive experiments and transgenic manipulation.Thus, Combined with the practice of our center , both the construction and the associated problems about the IVF-ET operating rooms were discussed in this paper .It will provide some help for the related researches .