Objective To investigate and observe the morphology of subcutaneous induced membrane and osteogenic factor expres-sion of the novel material calcium hydroxide-bone cement.Methods 15 4-week-old Specific Pathogen Free(SPF)female Kunming mice were randomly divided into three groups(n=5):experimental group,control group and blank group.A cut running deep under the skin was made on the back side,calcium hydroxide-bone cement was placed subcutaneously in the experimental group,the Polymethyl Methacrylate(PMMA)was placed subcutaneously in the control group,the blank group only with operation.After 6 weeks,the subcuta-neous tissue surrounded the material was taken from each group to be observed the pathological structure,calculated the collagen volume fraction(CVF)and detected the positive expression and relative expression of osteogenic factors such as bone morphogenetic protein-2(BMP-2),transforming growth factor-β1(TGF-β1)and vascular endothelial growth factor(VEGF)in the subcutaneous tissues.Results Comparing with the pathological sections of the blank group,there were induced membrane structure rich in collagenous fiber,microvessel and cell in subcutaneous tissue of the control and experimental groups.Comparing with control group,there were decrease of inflammatory cells,collagen content,microvessels,and disorder of collagenous fiber in experimental group.Comparing the CVF,average optical density(AOD)of osteogenic factors and grey scale value of protein bands in each group,the blank group,experimental group and control group increased sequentially,and there was a significant difference between each group(P＜0.05).Conclusion The new mate-rial,calcium hydroxide-bone cement,can form an induced membrane structure subcutaneously,but the 6-week induced membrane was not as good as the PMMA induced membrane during the same period in terms of morphology and osteogenic factor levels.

Objective:To investigate the clinical value of dynamic AI ultrasonic intelligent assisted diagnosis system for preoperative evaluation of thyroid nodules with diameter ≤1.0 cm.Methods:From Apr. 1, 2023, to Dec. 30, 2023, 742 thyroid nodules with diameter ≤1.0 cm were removed from 532 patients with thyroid nodule disease who received surgical treatment in the Department of Thyroid (hernia) of the First Medical Center of the Chinese People’s Liberation Army General Hospital. Among them, 423 were d≤0.5 cm. 319 cases (235 males and 507 females) with 0.5

Objective:To investigate the clinical value of dynamic AI ultrasonic intelligent assisted diagnosis system for preoperative evaluation of thyroid nodules with diameter ≤1.0 cm.Methods:From Apr. 1, 2023, to Dec. 30, 2023, 742 thyroid nodules with diameter ≤1.0 cm were removed from 532 patients with thyroid nodule disease who received surgical treatment in the Department of Thyroid (hernia) of the First Medical Center of the Chinese People’s Liberation Army General Hospital. Among them, 423 were d≤0.5 cm. 319 cases (235 males and 507 females) with 0.5

Objective To investigate and observe the morphology of subcutaneous induced membrane and osteogenic factor expres-sion of the novel material calcium hydroxide-bone cement.Methods 15 4-week-old Specific Pathogen Free(SPF)female Kunming mice were randomly divided into three groups(n=5):experimental group,control group and blank group.A cut running deep under the skin was made on the back side,calcium hydroxide-bone cement was placed subcutaneously in the experimental group,the Polymethyl Methacrylate(PMMA)was placed subcutaneously in the control group,the blank group only with operation.After 6 weeks,the subcuta-neous tissue surrounded the material was taken from each group to be observed the pathological structure,calculated the collagen volume fraction(CVF)and detected the positive expression and relative expression of osteogenic factors such as bone morphogenetic protein-2(BMP-2),transforming growth factor-β1(TGF-β1)and vascular endothelial growth factor(VEGF)in the subcutaneous tissues.Results Comparing with the pathological sections of the blank group,there were induced membrane structure rich in collagenous fiber,microvessel and cell in subcutaneous tissue of the control and experimental groups.Comparing with control group,there were decrease of inflammatory cells,collagen content,microvessels,and disorder of collagenous fiber in experimental group.Comparing the CVF,average optical density(AOD)of osteogenic factors and grey scale value of protein bands in each group,the blank group,experimental group and control group increased sequentially,and there was a significant difference between each group(P＜0.05).Conclusion The new mate-rial,calcium hydroxide-bone cement,can form an induced membrane structure subcutaneously,but the 6-week induced membrane was not as good as the PMMA induced membrane during the same period in terms of morphology and osteogenic factor levels.

Objective:To investigate the effect of different drainage methods on pancreatic fistula after pancreaticoduodenectomy (PD).Methods:The data of all patients with PD in Xijing Hospital, the First Affiliated Hospital of the Fourth Military Medical University from January 2007 to December 2018 were retrospectively analyzed. A total of 670 patients were enrolled, including 415 males and 255 females, aged (58.4±7.3) years, ranging from 24 to 82 years. According to the different method of pancreatic duct drainage, the propensity score was matched, and the patients were divided into internal drainage group ( n=529) and external drainage group ( n=141). The pancreatic fistula rate was compared between the two groups. Factors influencing pancreatic fistula after PD were analyzed by univariate and multivariate logistic regression. Results:The incidence of pancreatic fistula in the internal drainage group was 12.5% (66/529), which was significantly higher than that in the external drainage group 6.4% (9/141) (χ 2= 4.16, P=0.041). Multivariate logistic regression analysis showed that age ≥65 years ( OR=2.004, 95% CI: 1.185-3.390), complicated with digestive diseases ( OR=3.087, 95% CI: 1.599-5.959), history of upper abdominal surgery ( OR=2.031, 95% CI: 1.104-3.734) increased the risk of pancreatic fistula after PD (all P<0.05), decreased the risk of pancreatic fistula after PD in patients with external drainage ( OR=0.470, 95% CI: 0.223-0.989, P=0.047), and decreased the risk of pancreatic fistula after PD with the tumor size ( OR=0.725, 95% CI: 0.556-0.944, P=0.017), tumor located in the common bile duct after PD increased the risk of pancreatic fistula ( OR=1.497, 95% CI: 1.192-1.880, P=0.001). Conclusions:Compared with pancreatic duct drainage, external pancreatic duct drainage is better because of preventing postoperative pancreatic fistula.

Objective To compare the long-term efficacies of anterior cervical decompression and fusion (ACDF)and conservative therapy in treatment of unisegmental cervical disc herniation and to analyze the degree of the long-term adjacent segment disc degeneration in ACDF.Methods 120 patients treated in our hospital from January 2006 to January 2009 were selected,60 patients underwent ACDF as operation group and 60 patients underwent physical therapy and drug (conservative therapy)as non-operation group. All the patients were recorded when they were diagnosed with cervical unisegmental cervical disc herniation, and followed-up for 60 months, and they were evaluated with American Spinal Injury Association (ASIA)score,Visual Analogue Scale (VAS),Japanese Orthopedics Association (JOA) score and imaging (Miyazaki grading system). Results 107 patients were followed up,55 patients treated by operation were followed up for an average 59.7 months (59.7±0.4),while 52 patients treated by conservative therapy were followed up for an average 58.8 months (58.8±1.5).Compared with before treatment, the ASIA, JOA, and VAS scores of the patients in two group after treatment were improved (P<0.05),and the scores of ASIA,VAS,and JOA in operation group were superior to that in non-operation group (P<0.05),and adjacent segment disc degeneration occurred more frequently in the upper adjacent segment than the lower adjacent segment (P<0.05).Conclusion ACDF is superior to conservative therapy in alleviating symptoms and improving prognosis of unisegmental cervical disc herniation. Long-term postoperative disc degeneration mainly occurrs in the upper segment.