Objective:To investigate the risk factors for aggravation of type 2 diabetic foot ulcer and the correlation between the level of infection and the severity of the disease,as well as the association of the detection rate of bacteria and the types of bacteria with disease severity in patients with type 2 diabetic foot ulcer,and to provide individualized guidance for the prevention,diagnosis,and treatment of type 2 diabetic foot.Methods:A retrospective analysis was performed for the data of 150 patients with type 2 diabetic foot who were treated in Department of Endocrinology,The First Affiliated Hospital of Kunming Medical University,from October 2021 to June 2023,and according to the Wagner grade of diabetic foot,these patients were divided into mild group A(35 patients with Wagner grade 2),moderate group B(58 patients with Wagner grade 3),and severe group C(57 patients with Wagner grade 4).The clinical data of the three groups were analyzed.Results:The severity of type 2 diabetic foot ulcer was correlated with white blood cell count,neutrophil count,platelet count,monocyte count,globulin,alkaline phosphatase,neutrophil-lymphocyte ratio,monocyte-lymphocyte ratio,platelet-lymphocyte ratio,high-sensitivity C-reactive protein,procalcitonin,interleukin-6,erythrocyte sedimentation rate,hemoglo-bin,albumin/globulin ratio,lymphocyte count,cholinesterase,albumin,fasting blood glucose,gamma-glutamyl transferase,glycated he-moglobin,blood uric acid,high-density lipoprotein cholesterol,total cholesterol,triglycerides,and low-density lipoprotein cholesterol(P<0.05).The multivariate ordinal logistic regression analysis showed that the neutrophil-lymphocyte ratio and erythrocyte sedi-mentation rate were independent risk factors for the progression of Wagner grade of diabetic foot(P<0.05).For groups A,B,and C,the detection rate of bacteria in foot ulcer secretion tended to increase with the severity of diabetic foot,with the highest detection rate in group C,followed by groups B and A.A total of 31 types of bacteria were detected in the three groups,and the proportion of Gram-positive bacteria was higher than that of Gram-negative bacteria,with Staphylococcus aureus accounting for the highest proportion of 27.72%in all samples.Conclusion:High-sensitivity C-reactive pro-tein,procalcitonin,interleukin-6,and erythrocyte sedimentation rate increase with the progression of the Wagner grade of diabetic foot,and neutrophil-lymphocyte ratio and erythrocyte sedimentation rate are independent risk factors for the progression of the Wagner grade of diabetic foot.The detection rate of bacteria in foot ulcer secretion tends to increase with the Wagner grade of diabetic foot,and Gram-positive bacteria account for a higher proportion than Gram-negative bacteria,with Staphylococcus aureus accounting for the highest proportion.

Objective:To explore the factors influencing the supportive communication ability of medical undergraduates, and to propose strategies to improve supportive communication.Methods:By cluster sampling, we selected 388 medical undergraduates of grades 2017 and 2018 from Harbin Medical University for a questionnaire survey on supportive communication, general self-efficacy, and health education abilities. SPSS 22.0 was used for descriptive statistical analysis. AMOS 22.0 was used to construct a structural equation model to verify the relationship between the three variables. Mediating effects were also tested.Results:The students showed good supportive communication ability, with a total score of (74.28±10.84) points. The general self-efficacy score was (27.81±5.58) points, and the total score of health education ability was (25.50±4.76) points. General self-efficacy had direct positive effects on supportive communication and health education abilities ( β=0.75, 0.31, both P<0.001). Health education ability had a direct positive effect on supportive communication ability ( β=0.14, P<0.001). Health education ability played a significant mediating role in the influence of general self-efficacy on supportive communication ability (standardized mediating effect value=0.042, P<0.01), with the mediating effect accounting for 5.1%. Conclusions:The health education competency of medical undergraduates can mediate the effect of general self-efficacy on supportive communication ability. By strengthening medical humanities education to increase general self-efficacy and also emphasizing the cultivation of health education competency, the supportive communication ability of students can be improved.

Objective:To examine the efficacy and experience of staged and segmented two hybrid surgeries for total repair of Debakey type Ⅰ aortic dissection (TIAD).Methods:This study was a retrospective case series. The clinic data of 10 patients with acute TIAD who were admitted to the Department of Cardiac Surgery, Second Hospital of Lanzhou University or the First People′s Hospital of Lanzhou, between January 2016 and August 2022, were retrospectively studied. Ten patients underwent hybrid surgeries in two hospitalizations (stages), including 7 males and 3 females with an age of (60±7) years (range: 49 to 71 years). In stage 1, the first type Ⅱ hybrid arch repair was performed to treat the ascending, total arch, and descending thoracic aorta for acute TIAD without circulatory arrest. In stage 2, the second hybrid surgery including infrarenal abdominal aorta replacement, visceral arteries bypass and endovascular thoracoabdominal aortic repair was performed to treat residual thoracoabdominal aortic dissection after the first hybrid operation (segmented). Basic data, preoperative concomitant diseases, high-risk factors, surgical approaches and postoperative complications of all important organs, as well as CT imaging were analyzed.Results:There was no death in the 20 hybrid surgical procedures. In stage 1 type Ⅱ hybrid surgery, 4 cases underwent reconstruction of the aortic sinutubular junction, while Bentall and David surgery was performed for 3 cases, respectively. A patient received coronary artery bypass grafting. Then all patients were sequentially treated with arch debranching and thoracic aortic endovascular repair. Postoperative complications included renal insufficiency (4/10), hemofiltration (1/10), hypoxemia (4/10), neurologic event (1/10) and type Ⅱ endoleak (1/10). Complete false lumen thrombosis occurred in 9/10 of the patients. All complications recovered successfully at discharge and the average hospital stay was (21±4) days (range: 16 to 28 days) in the first hospitalization. At stage 2, the second hybrid surgery was successfully performed in all patients. No paraplegia, hepatic or renal insufficiency, or endoleak occurred. However, branch graft embolism of the left renal artery was found in one patient 3 days after laparotomy, as well as of superior mesenteric artery in another. Superior mesenteric artery occlusion was successfully treated by endovascular recanalization. Complete false lumen thrombosis occurred in all patients. Although all patients had different degrees of intestinal dysfunction, they were gradually relieved at discharge, and the average hospital stay was (19±2)days (range:16 to 21 days) in the second hospitalization. During follow-up, CT angiography showed aortic remodeling in all patients.Conclusion:Staged and segmented two hybrid surgeries are safe and feasible for total repair of Debakey type Ⅰ aortic dissection and are associated with acceptable early and midterm outcomes.

【Objective】 To explore the relationship between the methylation level of CNR1 and autism spectrum disorder (ASD), in order to provide a theoretical basis for the etiology of ASD. 【Methods】 A case-control study was conducted, recruiting 30 children with ASD from the Child Development and Behavior Research Center of Harbin Medical University and a rehabilitation facility, and 30 matched typically developed children from June 2017 to December 2018. The methylation levels of CNR1 in peripheral blood were measured by the Agena MassArray^® Mass Spectrometry System. A univariate conditional Logistic regression model was used to analyze the potential association between the methylation level of CNR1 and the risk of ASD with adjustment for age, BMI, body fat percentage and body fat. The correlations between the methylation level of CNR1 and the score of Social Responsiveness Scale (SRS) were evaluated by Pearson/Spearman correlation analysis. 【Results】 The methylation levels of the average methylation (t=2.224), CpG_3.4 (Z=2.187), CpG_9.10.11 (t=2.308), and CpG_28.29 (t=2.943) of the CNR1 promoter region in ASD children were significantly higher than controls (P<0.05). The methylation levels of the average methylation (OR=1.117, 95%CI: 1.003 - 1.245), CpG_9.10.11 (OR= 1.072, 95%CI:1.006 - 1.142), and CpG_28.29 (OR=1.078, 95%CI: 1.018 - 1.141) of the CNR1 promoter region were positively correlated with the risk of ASD (P<0.05). The methylation level of CpG_28.29 in ASD children was positively correlated with the scores of social motivation in SRS (r=0.421, P<0.05). 【Conclusions】 The methylation levels of CNR1 in peripheral blood are abnormal in ASD children and might be correlated with the risk of ASD and social function. The underlying mechanism needs to be further explored.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.

Objective:From the perspective of behavioral economics,it analyzes the reasons for the low utilization rate of palliative care and propose intervention measures,to promote the acceptance and application of palliative care by terminal cancer patients and their families.Methods:Using the theories and methods of behavioral economics to explore how cognitive and behavioral biases lead to irrational medical decisions in the choice of palliative care;based on the dual-process theory and nudge method,it aims to construct an intervention model for"palliative"care decision-making and propose intervention strategies.Results:Terminal cancer patients and their families with bounded rationality tend to prefer short-term aggressive treatment plans while making decisions,and use the extension of survival as the decision anchor point,weakening the influence of quality of life factors.The lack of sound mechanisms related to palliative care will further increase the cognitive bias of cancer patients and their families,putting them in a negative choice framework and risking losses,leading to the abandonment of more rational palliative care plans.Conclusion:By adopting the"perceptual-rational"thinking from the dual-process theory,transforming inter-temporal selection into the most cost-effective option,anchoring treatment expectations to patients'quality of life,increasing the value of palliative care benefits,and constructing a supportive selection framework as a target for intervention,it can help promote the use of palliative care.