Objective To evaluate the predictive and diagnostic value of plasma interleukin-6 (IL-6) and soluble suppression of tumorigenicity 2 (sST2) for acute respiratory distress syndrome (ARDS) in patients with abdominal sepsis. Methods A retrospective study method was adopted,clinical data from patients with abdominal sepsis admitted to the department of critical care medicine of China-Japan Friendship Hospital,between January 2022 and December 2023 were collected. These data included gender,age,sST2,IL-6,procalcitonin (PCT),C-reactive protein (CRP),white blood cell count (WBC),neutrophil ratio,hemoglobin (Hb),platelet count (PLT),lactate dehydrogenase (LDH),pH value,arterial partial pressure of oxygen (PaO2),fraction of inspired oxygen (FiO2),arterial partial pressure of carbon dioxide (PaCO2),oxygenation index (PaO2/FiO2),arterial blood lactate acid (Lac),prothrombin time (PT),activated partial thromboplastin time (APTT),D-dimer,acute physiology and chronic health evaluationⅡ(APACHEⅡ) score,and sequential organ failure assessment (SOFA) score. Patients were divided into two groups based on the presence or absence of ARDS. The differences in the above indices between the two groups were analyzed. Spearman correlation analysis was used to examine the relationship between plasma IL-6 and sST2 levels and clinical indicators in the ARDS group. Multivariate Logistic regression was conducted to identify risk factors for ARDS in patients with abdominal sepsis,and receiver operator characteristic curve (ROC curve) were used to assess the predictive value of IL-6 and sST2 for ARDS. Results A total of 89 patients with abdominal sepsis were included,with 41 in the ARDS group and 48 in the non-ARDS group. Compared with the non-ARDS group,the ARDS group showed significantly higher levels of sST2,IL-6,PaCO2,APACHEⅡ score,SOFA score,and FiO2[sST2 (μg/L):733.7 (370.1,1609.1) vs. 86.1 (64.1,129.1),IL-6 (ng/L):1106.79 (405.37,3848.00) vs. 101.00 (44.00,396.00),PaCO2 (mmHg,1 mmHg≈0.133 kPa):40.00 (35.50,45.50) vs. 36.00 (31.85,40.73),APACHEⅡ score:31.7±1.0 vs. 28.6±0.9,SOFA score:10.30±0.50 vs. 8.17±0.70,FiO2:0.600 (0.500,1.000) vs. 0.400 (0.400,0.575),all P<0.05],APTT was significantly prolonged in the ARDS group[s:49.70 (41.95,56.43) vs. 43.30 (39.40,49.60)],pH value,PaO2 and PaO2/FiO2 were significantly decreased[pH value:7.37 (7.30,7.43) vs. 7.41 (7.35,7.47),PaO2 (mmHg):82.00 (68.00,107.00) vs. 119.50 (101.25,154.00),PaO2/FiO2 (mmHg):157.00 (99.10,200.00) vs. 297.56 (228.00,386.00),all P<0.05]. Spearman correlation analysis showed in the sepsis-related ARDS group,plasma IL-6 was positively correlated with PCT,SOFA score,and Lac (r were 0.437,0.470,and 0.374,respectively;P value were 0.005,0.002,and 0.016,respectively),plasma sST2 levels were also positively correlated with PCT,SOFA score,and Lac (r were 0.492,0.454,and 0.441,respectively;P value were 0.009,0.013,and 0.015,respectively). Multivariate Logistic regression analysis indicated that plasma sST2 and IL-6 had diagnostic value for ARDS in patients with abdominal sepsis,with area under the curve (AUC) values of 0.978 and 0.719,respectively,and optimal cut-off values of 246.9 μg/L and 397.8 ng/L,both P<0.05,sensitivities were 86.2％ and 79.3％,and specificities were 100.0％ and 85.0％ for sST2 and IL-6,respectively. Conclusion Plasma sST2 and IL-6 are closely associated with ARDS in patients with abdominal sepsis and have high diagnostic value for identifying ARDS in this population.

Objective To explore the effect of sorafenib on HeLa cell proliferation by inducing cell apoptosis and autophagy and its impact on drug resistance.Methods The drug-resistant cell strains were constructed through in-termittent induction method,with concentrations of 0,2.5,5.0,7.5,10.0,15.0,20.0 μmol/L.HeLa cells were incubated with increasing concentrations of sorafenib with each concentration for 1 week.The drug-resistant cell strains with stable passages were collected.MTT assay was used to detect the effect of sorafenib on cell prolifer-ation.Cell cycle distribution was analyzed by flow cytometry.The change in the expression of drug-resistant and ap-optotic genes in the parents and drug-resistant cell strains under different drug concentrations was examined by semi-quantitative PCR.The changes of apoptotic related marker proteins LC3-Ⅰ and LC3-Ⅱ were detected by Westernblot.Results Stable drug-resistant strains were successfully obtained;Drug-treated cells were more blocked in the G1 phase.In drug-resistant cells,the expression of apoptosis suppressor gene Bcl-2 was significantly decreased and the apoptotic gene Bax as well as the drug-resistant genes were all significantly increased(P＜0.05).The LC3-Ⅱ/LC3-Ⅰ ratio of drug-resistant cells was significantly higher than that of parent cells(P＜0.05).Conclusions Sorafenib may block the cell cycle,suppress malignant cell proliferation and promote autophage.On one hand,autophagy participates in the development of cell drug resistance and promotes cell survival.On the other hand,drug-induced autophagy may activate some of apoptotic signaling pathway in drug-resistant cells and promote the reversal of cell drug resistance.

Objective To evaluate the predictive and diagnostic value of plasma interleukin-6 (IL-6) and soluble suppression of tumorigenicity 2 (sST2) for acute respiratory distress syndrome (ARDS) in patients with abdominal sepsis. Methods A retrospective study method was adopted,clinical data from patients with abdominal sepsis admitted to the department of critical care medicine of China-Japan Friendship Hospital,between January 2022 and December 2023 were collected. These data included gender,age,sST2,IL-6,procalcitonin (PCT),C-reactive protein (CRP),white blood cell count (WBC),neutrophil ratio,hemoglobin (Hb),platelet count (PLT),lactate dehydrogenase (LDH),pH value,arterial partial pressure of oxygen (PaO2),fraction of inspired oxygen (FiO2),arterial partial pressure of carbon dioxide (PaCO2),oxygenation index (PaO2/FiO2),arterial blood lactate acid (Lac),prothrombin time (PT),activated partial thromboplastin time (APTT),D-dimer,acute physiology and chronic health evaluationⅡ(APACHEⅡ) score,and sequential organ failure assessment (SOFA) score. Patients were divided into two groups based on the presence or absence of ARDS. The differences in the above indices between the two groups were analyzed. Spearman correlation analysis was used to examine the relationship between plasma IL-6 and sST2 levels and clinical indicators in the ARDS group. Multivariate Logistic regression was conducted to identify risk factors for ARDS in patients with abdominal sepsis,and receiver operator characteristic curve (ROC curve) were used to assess the predictive value of IL-6 and sST2 for ARDS. Results A total of 89 patients with abdominal sepsis were included,with 41 in the ARDS group and 48 in the non-ARDS group. Compared with the non-ARDS group,the ARDS group showed significantly higher levels of sST2,IL-6,PaCO2,APACHEⅡ score,SOFA score,and FiO2[sST2 (μg/L):733.7 (370.1,1609.1) vs. 86.1 (64.1,129.1),IL-6 (ng/L):1106.79 (405.37,3848.00) vs. 101.00 (44.00,396.00),PaCO2 (mmHg,1 mmHg≈0.133 kPa):40.00 (35.50,45.50) vs. 36.00 (31.85,40.73),APACHEⅡ score:31.7±1.0 vs. 28.6±0.9,SOFA score:10.30±0.50 vs. 8.17±0.70,FiO2:0.600 (0.500,1.000) vs. 0.400 (0.400,0.575),all P<0.05],APTT was significantly prolonged in the ARDS group[s:49.70 (41.95,56.43) vs. 43.30 (39.40,49.60)],pH value,PaO2 and PaO2/FiO2 were significantly decreased[pH value:7.37 (7.30,7.43) vs. 7.41 (7.35,7.47),PaO2 (mmHg):82.00 (68.00,107.00) vs. 119.50 (101.25,154.00),PaO2/FiO2 (mmHg):157.00 (99.10,200.00) vs. 297.56 (228.00,386.00),all P<0.05]. Spearman correlation analysis showed in the sepsis-related ARDS group,plasma IL-6 was positively correlated with PCT,SOFA score,and Lac (r were 0.437,0.470,and 0.374,respectively;P value were 0.005,0.002,and 0.016,respectively),plasma sST2 levels were also positively correlated with PCT,SOFA score,and Lac (r were 0.492,0.454,and 0.441,respectively;P value were 0.009,0.013,and 0.015,respectively). Multivariate Logistic regression analysis indicated that plasma sST2 and IL-6 had diagnostic value for ARDS in patients with abdominal sepsis,with area under the curve (AUC) values of 0.978 and 0.719,respectively,and optimal cut-off values of 246.9 μg/L and 397.8 ng/L,both P<0.05,sensitivities were 86.2％ and 79.3％,and specificities were 100.0％ and 85.0％ for sST2 and IL-6,respectively. Conclusion Plasma sST2 and IL-6 are closely associated with ARDS in patients with abdominal sepsis and have high diagnostic value for identifying ARDS in this population.

Objective:To explore the efficacy of subcutaneous injection of granulocyte-macrophage colony-stimulating factor (GM-CSF) in preventing invasive fungal disease (IFD) in patients with multiple myeloma (MM).Methods:The clinical data of 222 patients who were admitted to the Second Hospital of Harbin Medical University from January 2015 to June 2021 were retrospectively analyzed. The patients was given GM-CSF (3-5 μg·kg -1·d -1, GM-CSF group) or granulocyte colony-stimulating factor (G-CSF, 2-5 μg·kg -1·d -1, G-CSF group) when neutrophils (ANC) ≤1.5×10 9/L after induction chemotherapy. Patients were discontinued when white blood cell count (WBC) ≥10.0×10 9/L. The incidence of IFD (including confirmed, clinical and proposed diagnosis) and breakthrough invasive fungal infections was compared between the two groups. Results:The incidence of IFD was 8.1% (18/222) in all patients. The incidence of IFD was 3.5% (3/85) and 10.9% (15/137) in the GM-CSF and G-CSF groups, respectively, and the difference between the two groups was statistically significant ( χ2 = 3.88, P = 0.049). In 9 patients of GM-CSF group receiving fungal infection prophylaxis and in 15 patients of G-CSF group receiving fungal infection prophylaxis, the incidence of breakthrough invasive fungal infections was 0 and 7 cases, respectively, and the difference between the two groups was statistically significant ( P = 0.022). Conclusions:GM-CSF application in MM patients can reduce the incidence of IFD and breakthrough invasive fungal infections.

Four patients with coexistence of sarcoidosis and primary Sj(o)gren syndrome (pSS) were retrospectively analyzed.All patients were female,who were referred to our department mainly because of respiratory symptoms.Positive antinuclear antibody (ANA) was detected in 2 patients and anti-Sj(o)grens syndrome A (SSA) antibody positive in 1 patient.All patients presented specific histologic patterns of both sarcoidosis and pSS.Publications related to coexistence of these two diseases were reviewed.Forty-one patients were finally included in the analysis,among whom 37 confirmed patients were from literature search.There were 37 women and 4 men.The main clinical features presentation were xerophthalmia in 40,xerostomia in 38,hilaradenopathies in 28,interstitial lung disease in 15,respiratory symptoms in 13.The main immunologic data were positive ANA in 23,SSA antibody in 19,anti-Sj(o)grens syndrome B antibody in 10 and rheumatoid factor in 12.All patients presented specific histologic patterns of both diseases.Patients with both sarcoidosis and pSS of ten represent multisystemic involvement and positive immunologic parameters,as well as the dual expression of specific histologic characteristics.

Three female patients admitted with elevated creatine kinase,impaired muscle on electromyogram,and positive repetitive nerve stimulation and neostigmine tests were diagnosed as polymyositis (PM) with myasthenia gravis (MG).Twenty five more cases were retrieved by literature search,and the clinical data of total 28 cases were analyzed.There were 10 males and 18 females with an average age of 56 years.The clinical manifestations include dyspnea(43％),dysphagia(43％),ptosis (43％),dysarthria(29％),diplopia(18％),cough after drinking(14％),myalgia(11％).Thirteen out of 26 cases (50％) had positive results in repetitive nerve stimulation (RNS) and 10/11 showed positive reaction in neostigmine test.Serum positive anti-acetylcholine receptor was detected in 21 out 23 patients (91％).

To investigate the characteristics of traditional Chinese medicine (TCM) syndromes and their elements in people with subhealth fatigue.